Microbiological, clinical and 3-dimensional features of white spot lesions
White spot lesion (WSL) development in the enamel surface is by far the most important iatrogenic effect of fixed orthodontic therapy. One study found that 50% of individuals undergoing fixed orthodontic treatment had a WSL compared with 25% of controls. Little is known about the microflora, clinical variation and 3-dimensional structure of WSLs.
Aims of the present study
- Use molecular microbiological techniques such as the HOMIM microarray to characterize the bacterial flora present prior to, and after bonding of a fixed orthodontic appliance that produces WSLs
- Characterize the bacterial flora present prior to and during deliberate WSL formation in vivo by using specially designed orthodontic bands
- Quantify the color of WSLs and relate it to the bacteria found by HOMIM microarray
- Determine the 3D structure of WSLs and relate it to the bacteria found by HOMIM