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Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Show all 10 contributors for this article]
(2022).
Orale soppinfeksjoner - mer enn Candida albicans: mikrobiologiske analyser av prøver fra alvorlig syke pasienter med kreft .
Show summary
Bakgrunn
Pasienter med alvorlig kreftsykdom er utsatte for orale soppinfeksjoner, grunnet sykdommen, kreftbehandling og/eller svekket immunforsvar. Orale soppinfeksjon påvirker pasienters matlyst, ernæringstilstand og livskvalitet. Candida albicans er en vanlig soppart i munnhulen, men forekomst av non-albicans Candida-arter, og i kombinasjon med C. albicans, er økende. Artene har varierende toleranse for antimykotiske midler, det kan gi behandlingsutfordringer.
Hensikten med studien var å identifisere og resistensteste Candida sopparter i munnhulen til alvorlig syke pasienter med kreft.
Metode
Sopprøver tatt under studien Oral Health in Advanced Cancer i perioden 2014 – 2016, ble analysert høsten 2020. Pasientprøvene ble re-dyrket ved Institutt for oral biologi, Universitetet i Oslo. Soppartene ble identifisert biokjemisk og ved DNA sekvensering, og resistenstestet for antimykotiske legemidler: fluconazole, amphotericin B, anidulafungin og nystatin.
Resultat
Vi analyserte totalt 56 isolater fra 45 pasienter og identifiserte syv Candida arter og en Saccharomyces. Til sammen hadde 36 pasienter oppvekst av én soppart, og 9 pasienter hadde 2 til 3 ulike sopparter i munnhulen. Av C. albicans artene var 39 av 40 følsomme for fluconazole. To Candida parapsilosis og en Candida tropicalis var resistent mot anidulafungin. En C. tropicalis var resistent mot anidulafungin. En Candida glabrata var resistent mot fluconazole.
Konklusjon
Studien viste at forskjellige Candida arter forekommer både i monoinfeksjoner og blandingsinfeksjoner. C. albicans var dominerende med høy følsomhet for antimykotiske midler. Blandingsinfeksjoner kan komplisere behandlingen og forårsake gjentagende infeksjoner. Mikrobiologiske prøver og analyser med identifisering og resistenstesting, bør være en del av utredningen hos pasienter med alvorlig og tilbakevendende orale soppinfeksjoner.
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Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Show all 10 contributors for this article]
(2022).
Oral candidiasis in advanced cancer – identification and susceptibility testing of detected Candida species.
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Introduction: Candida albicans is the most common fungal species in the oral cavity. Increased prevalence of non-C. albicans are detected, and co-infection with C. albicans may have implications for treatment, due to antifungal resistance. The aim of the study was to assess the diversity and susceptibility of oral yeasts in patients with advanced cancer.
Methods: Clinical fungal isolates were sampled in 2014-2016 from 45 patients in a hospice unit. Totally 56 isolates were grown on chromID Candida plates, prepared for biochemical identification, and controlled with DNA sequencing. E-test was performed with the antifungal agents fluconazole, amphotericin B, anidulafungin and nystatin were tested.
Results: A total of 56 isolates were identified. Candida species was dominated by C. albicans at 71%, followed by C. glabrata 9%, C. parapsilosis 5%, C. tropicalis 4%, C. krusei 2%, C. guilliermondii 2% and C. dubliniesis 2%. Nine patients had 2-3 different species. C. albicans strains were susceptible to fluconazole in 97.5%. One C. parapsilosis and one C. tropicalis strain were resistant to anidulafungin. One C. glabrata was resistant to fluconazole and another was just below the threshold for resistance. The sequencing confirmed the biochemical identification.
Conclusion: C. albicans was the most prevalent species. Variation in susceptibility was observed in patients with mixed colonies. Combinations of Candida species and the lack of identification and susceptibility testing may lead to inadequate treatment.
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Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Show all 10 contributors for this article]
(2022).
Identification and susceptibility testing of oral candidiasis in advanced cancer patients – an important diagnostic tool.
Show summary
Background: Candida albicans is the most common fungal species detected in the oral cavity. Studies of oral fungal samples have shown an increased prevalence of non-C. albicans species in mixed infection with C. albicans. Non-C. albicans and C. albicans are associated with varying degrees of resistance to azole. This may have implications for treatment, due to acquired antifungal resistance.
Aims: To assess the diversity and susceptibility of detected Candida species in the oral cavity in patients with advanced cancer.
Methods: Clinical fungal isolates sampled in 2014-2016 from 45 patients in a hospice unit were analyzed. A total of 56 isolates were grown on chromID Candida plates. After phenotypic identification by colour of the colonies, one single colony of each species was re-cultivated and prepared for identification on a VITEK2® system. Susceptibility testing with E-test was performed on RPMI agar, and the antifungal agents fluconazole, amphotericin B, anidulafungin and nystatin were tested.
Results: A total of 56 Candida isolates were identified. The diversity of Candida species was dominated by C. albicans at 89%, followed by C. glabrata 11%, C. parapsilosis 7%, C. tropicalis 4%, C. krusei 2%, C. guilliermondii 2% and C. dubliniesis 2%. Thirty-six patients had monoculture, and nine patients had 2-3 different species detected. Almost all C. albicans strains (97.5%) were susceptible to fluconazole. One C. parapsilosis and one C. tropicalis strain were resistant to anidulafungin. One C. glabrata was resistant to fluconazole and another was just below the threshold for resistance.
Conclusion: C. albicans was the most prevalent Candida species in our sample. Variation in susceptibility was observed in patients with both C. albicans and C. glabrata. Combinations of Candida species and the lack of identification and susceptibility testing may lead to inadequate treatment and accelerate the development of resistance.
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Rogala, Agnieszka; Kristoffersen, Anne Karin; Enersen, Morten; Sapkota, Dipak & Küntziger, Thomas M.
(2021).
Defining the role of Porphyromonas gingivalis’ peptidyl arginine deiminase (PPAD) in oral carcinoma.
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Lund, Bodil Kristina & Enersen, Morten
(2020).
Akutte odontogene infeksjoner. Marginal periodontitt.
Nasjonale Faglige Retningslinjer for Antibiotikabruk i Primærhelsetjenesten. Kapittel 6. Helsedirektoratet.
Nasjonale Faglige Retningslinjer for Antibiotikabruk i Primærhelsetjenesten.
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Enersen, Morten
(2019).
Antibiotika og smittevern i tannlegens hverdag.
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Rogala, Agnieszka; Follin-Arbelet, Benoit; Kristoffersen, Anne Karin; Enersen, Morten; Sapkota, Dipak & Küntziger, Thomas M.
(2019).
Defining the role of Porphyromonas gingivalis peptidyl arginine deiminase (PPAD) in oral carcinoma.
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Bentele, Horst; Enersen, Morten; Eriksen, Hanne-Merete; Hensten, Arne; Lochner, Vibeke & Lund, Bodil Kristina
[Show all 9 contributors for this article]
(2018).
Faglige anbefalinger for smittevern i klinisk odontologisk praksis.
Show summary
Dette dokumentet er en nasjonal anbefaling for smittevern i odontologisk praksis.
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Rusthen, Shermin; Kristoffersen, Anne Karin; Young, Alix; Galtung, Hilde; Enersen, Morten & Jensen, Janicke Liaaen
(2018).
The Salivary Microbiota Profile in Reduced and Normal Salivation.
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Handal, Trude & Enersen, Morten
(2017).
Antibiotikabruk i primærhelsetjenesten
Tannhelse, 1) Akutte odontogene infeksjoner;
2) Marginal periodontitt.
Antibiotikabruk i Primærhelsetjenesten.
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Enersen, Morten
(2017).
Superinfections; are they on the way?
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Håheim, Lise Lund; Rønningen, Kjersti Skjold; Enersen, Morten & Olsen, Ingar
(2017).
Tooth extractions, oral infections, and hs-C-reactive protein predict mortality in individuals with and without diabetes.
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Enersen, Morten
(2016).
Antibiotikaresistens og smittevern i klinisk odontologi.
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Enersen, Morten
(2016).
Nasjonal handlingsplan mot antibiotikaresistens 2015-2020. Ikke bare for medisinerne.
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Enersen, Morten
(2016).
Antibiotikaresistente mikrober i tannlegens hverdag.
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Enersen, Morten
(2016).
Antibiotika ved odontogene infeksjoner.
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Enersen, Morten
(2016).
Antibiotikabruk, antibiotikaresistens og smittevern med økende forekomst av resistente bakterier. Hvordan kan tannlegene bidra?
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Langbach, Odd; Kristoffersen, Anne Karin; Abesha-Belay, Emnet; Enersen, Morten & Olsen, Ingar
(2016).
Nett i bukveggen hos brokkopererte inneholder orale mikrober.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
126(8),
p. 610–611.
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Enersen, Morten
(2016).
Antibiotika ved odontogene infeksjoner. Teori og klinisk anvendelse i lys av økende resistensutvikling.
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Danielsen, Sigurd Haugan; Handal, Trude & Enersen, Morten
(2015).
Marginal periodontitt.
Antibiotikabruk i Primærhelsetjenesten.
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Danielsen, Sigurd Haugan; Handal, Trude & Enersen, Morten
(2015).
Akutte odontogene infeksjoner.
Antibiotikabruk i Primærhelsetjenesten.
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Hallmer, Fredrik; Bjørnland, Tore; Kristoffersen, Anne Karin; Anderson, Gunilla & Enersen, Morten
(2015).
THE ASSOCIATION BETWEEN ORAL MICROFLORA AND BISPHOSPHONATE OR DENOSUMAB INDUCED OSTEONECROSIS - abstract
ICOMS 2015 - International Conference on Oral and Maxillofacial Surgery, Melbourne October 2015.
ICOMS 2015 - International Conference on Oral and Maxillofacial Surgery.
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Enersen, Morten
(2015).
Microbial diagnosis in the treatment of periodontal disease and periimplant infections.
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Enersen, Morten
(2015).
Microbiological diagnosis in the treatment of periodontal diseases and peri-implant infections. Validity and reliability with increasing antibiotic resistance.
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Enersen, Morten
(2015).
Mikrobiologisk diagnostikk ved odontogene infeksjoner. Antimikrobiell resistenstesting; er det nødvendig?
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Enersen, Morten
(2015).
Mikrobiologisk diagnostikk og periodontal behandling, er dette nødvendig?
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Enersen, Morten
(2015).
Antibiotika ved odontogene infeksjoner.
Teori og klinisk anvendelse i lys av økende resistensutvikling.
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Bunaes, Dagmar; Enersen, Morten; Aastrøm, A.N.; Mustafa, Kamal Babikeir Eln; Lie, S.A. & Leknes, Knut N.
(2015).
The influence of cigarette smoking on clinical and microbiological outcomes following primary periodontal treatment.
Journal of Clinical Periodontology.
ISSN 0303-6979.
42,
p. 3–3.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Show all 7 contributors for this article]
(2014).
Phenotypic characterization of Candida glabrata from blood, respiratory and gastrointestinal tracts.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Show all 7 contributors for this article]
(2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts.
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Enersen, Morten
(2014).
Antibiotika ved odontogene infeksjoner. Teori og klinisk anvendelse i lys av økende resistensutvikling.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Show all 7 contributors for this article]
(2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts. Poster presentation.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Show all 7 contributors for this article]
(2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts. Oral Presentation.
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Midtervoll, Ingvild K.; Enersen, Morten; Kristoffersen, Anne Karin; Samuelsen, Sven Ove; Olsen, Ingar & Håheim, L.L.
(2014).
Detection of Periodontal Pathogens in Blood from Patients with Cardiovascular Disease. Poster presentation.
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Midtervoll, Ingvild K.; Enersen, Morten; Kristoffersen, Anne Karin; Samuelsen, Sven Ove & Lund Håheim, Lise
(2014).
Detection of Periodontal Pathogens in Blood from Patients With Cardiovascular Disease. Oral Presentation.
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Zandi, Homan; Rodrigues, Renata C. V.; Kristoffersen, Anne Karin; Ørstavik, Dag; Siqueira, Jose F. & Rocas, Isabela
[Show all 7 contributors for this article]
(2014).
Clinical effectiveness of two antimicrobial irrigants in endodontically treated teeth. Poster presentation.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Show all 7 contributors for this article]
(2014).
Phenotypic diversity among clinical Candida glabrata strains in Norwegian health care unit. Oral presentation.
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Zandi, Homan; Rodrigues, Renata C. V.; Kristoffersen, Anne Karin; Ørstavik, Dag; Siqueira, Jose F. & Rocas, Isabela
[Show all 7 contributors for this article]
(2014).
Clinical effectiveness of two antimicrobial irrigants in endodontically treated teeth. Oral presentation.
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Clinical Effectiveness of Two Antimicrobial Irrigants in Endodontically Treated Teeth
Homan Zandi, DDS, Renata C. V. Rodrigues, DDS, Anne-Karin Kristoffersen, PhD, Dag Ørstavik, DDS, PhD, José F. Siqueira Jr, DDS, PhD, Isabela Rôças, DDS, PhD, Morten Enersen, DDS, PhD
Abstract
Background: Endodontic treatment of root-filled teeth with post-treatment disease is especially challenging. The outcome of endodontic treatment is significantly influenced by the presence of bacteria in the root canals at the time of filling. Sodium hypochlorite (NaOCl) is widely used as a root canal irrigant in different concentrations because of its pronounced antimicrobial activity and the ability of dissolving necrotic tissue and organic matter. Chlorhexidine gluconate (CHX) is an effective substitute for NaOCl due to its broad-spectrum antimicrobial efficacy against bacteria and its high dentin substantivity. It is less toxic against vital tissues than NaOCl but lacks tissue dissolving property. No culture-independent molecular study has compared the antimicrobial effectiveness of 1% NaOCl and 2% CHX in root-filled teeth with apical periodontitis (AP). Objectives: The aim of the present study was to compare the antibacterial properties of 1% NaOCl and 2% CHX in endodontically treated teeth with AP. Methods: Sixty-eight root-filled teeth with AP were randomly distributed in two irrigant groups. Bacterial samples were taken at baseline (S1), after chemomechanical preparation using either 1% NaOCl or 2% CHX as irrigant (S2), and after intracanal medication with calcium hydroxide at the second visit (S3). The bacterial samples were analyzed by 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) for total bacteria, Enterococcus faecalis and Streptococcus spp.. Results: Preliminary results indicated bacterial DNA in all the samples at S1. E. faecalis was seldom found, but streptococci were frequent. The amount of bacterial DNA significantly declined following treatment in both groups (S2 and S3) (p<0.001). There was no significant difference between NaOCl and CHX groups in reducing total bacterial and streptococcal counts (p>0.05). Conclusion: Findings indicate that endodontic retreatment was equally effective in reducing the amount of bacteria in both irrigation groups.
Keywords:
Endodontics. Root canal irrigants.
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Enersen, Morten
(2013).
Laser i periodontien, Kliniske forsøk- mikrobiologiske data.
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Midtervoll, Ingvild K.; Kristoffersen, Anne Karin; Enersen, Morten; Samuelsen, Sven Ove & Lund Håheim, Lise
(2013).
PERIODONTAL PATHOGENS – A POSSIBLE ROLE IN CARDIOVASCULAR DISEASE?
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Objective: The aim of this ongoing study is to investigate a possible role for periodontal pathogens in relation to cardiovascular disease (CVD) through detection of bacterial DNA in bloodsamples from participants of the Oslo II study.
Materials and methods: The study design is a blinded case-cohort design where the cohort is men from Oslo, born between 1926 and 1932. The test group consists of 225 individuals who have died from CVD (myocardial infarction, aortic aneurysms or stroke), and the control group are 225 healthy men randomly picked from the cohort. After DNA extraction from blood samples in both groups, PCR amplification of the 16S rRNA bacterial gene will be performed, and PCR products of 650bp fragment will be verified on a 1,5% agarose gel. Bacterial species will be detected by high throughput sequencing of the PCR products, and results from test and control group will be correlated. Furthermore, results from three diagnoses in the test group will also be correlated separately to the controls. Correction for known risk factors such as smoking, physical inactivity, high blood pressure, total serum cholesterol and diabetes will be performed.
Results: Preliminary results from PCR amplification of 260 blinded bloodsamples indicate bacterial DNA detection with the expected fragment size in 124 (48%) of the samples investigated so far.
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Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne-Karin; Gaustad, Peter; Ørtengren, Ulf Thore & Olsen, Ingar
(2012).
Phenotypic and genotypic characterization of Candida glabrata.
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Midtervoll, Ingvild Kristin; Kristoffersen, Anne-Karin; Enersen, Morten; Samuelsen, S. O.; Olsen, Ingar & Lund Håheim, Lise
(2012).
Systemic effects of periodontal pathogens.
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Enersen, Morten; Narverud, Ingunn; Mdala, Ibrahimu; Nenseter, Marit Sofie; Holven, Kirsten Bjørklund & Olsen, Ingar
(2011).
Inflammatory and hematological markers in patients with advanced chronic periodontitis.
Journal of Oral Microbiology.
ISSN 2000-2297.
3,
p. 3–3.
doi:
10.3402/jom.v3i0.7144.
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Enersen, Morten
(2011).
Periodontitt: Undersøkelse, strategier for systematisk behandling og vedlikehold.
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Enersen, Morten & Vandevska-Radunovic, Vaska
(2010).
Kjeveortopedi og periodonti - molekylære og kliniske aspekter i det interdisiplinære samarbeidet mellom to spesialiteter.
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Enersen, Morten
(2009).
Oppdatering i periodonti.
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Enersen, Morten
(2009).
Oppdatering i Periodonti, fagkurs.
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Enersen, Morten
(2009).
Orale infeksjoner; revisjon av dette kapittel til utgave 4.
In Degre, Miklos; Hovig, Berit & Rollag, Halvor (Ed.),
Medisinsk Mikrobiologi, 3.utg.
Gyldendal Akademisk.
ISSN 978-82-05-31590-7.
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Enersen, Morten; Olsen, Ingar & Caugant, Dominique A
(2008).
Genetiske analyser av bakterien Porphyromonas gingivalis fra periodontittt.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
118.
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Enersen, Morten
(2008).
Periodontal mikroflora og populasjonsgenetikk.
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Enersen, Morten; Olsen, Ingar; Kvalheim, Øyvind & Caugant, Dominique A
(2007).
FimA genotypes and multilocus sequence types of Porphyromonas gingivalis from periodontitis.
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Enersen, Morten
(2007).
Mikrobiologisk diagnostikk og periodontal behandling.
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Sunde, Pia Titterud; Olsen, Ingar; Enersen, Morten; Beiske, Klaus & Grinde, Bjørn
(2007).
Cytomegalovirus and Epstein-Barr virus in periodontitis: a role in pathology?
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Sunde, PT; Enersen, Morten; Olsen, Ingar & Grinde, Bjørn
(2006).
Does CMV or EBV contribute to periodontitis?
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Enersen, Morten; Olsen, Ingar & Caugant, Dominique A
(2006).
FimA genotypes and multilocus sequence types in Porphyromonas gingivalis.
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Enersen, Morten; Olsen, Ingar & Caugant, Dominique A
(2006).
FimA Genotypes and Multilocus Sequence Types in Porphyromonas gingivalis.
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Enersen, Morten; Olsen, Ingar & Caugant, Dominique A
(2006).
FimA genotypes and multilocus sequence types in Porphyromonas gingivalis.
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Enersen, Morten; Olsen, Ingar; Caugant, Dominique A & Van Winkelhoff, Arie
(2005).
Multilocus sequence typing of Porphyromonas gingivalis strains.
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Blix, Hege Salvesen & Enersen, Morten
(2018).
Antibiotic prescribing in dentistry.
Folkehelseinstituttet.
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Blix, Hege Salvesen; Torheim, Sissel Marie & Enersen, Morten
(2017).
Antibiotic prescribing in dentistry in
Norm/norm-Vet 2016; Usage of AntimicrobialAgents and Occurrence of Antimicrobial Resistance in Norway.
Folkehelseinstituttet, Veterinærinstituttet.
ISSN 1502-2307.
2016(1).
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Vikholt, Katharina Johnsen; Andersen, Kari-Mette; Kristoffersen, Anne Karin & Enersen, Morten
(2014).
Oral candidiasisand molecular epidemiology in Candida glabrata.
Universitetet i Oslo.
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Solli, Silje Jeanette; Nguyen, Toan Duy; Kristoffersen, Anne Karin & Enersen, Morten
(2014).
RgpB-genet; en viktig virulensfaktor hos den periopatogene bakteriearten Porphyromonas gingivalis. Et molekylærbiologisk studium.
Universitetet i Oslo.