Haugsten, Helene Rygvold; Kristoffersen, Anne Karin; Haug, Trude Marie; Søland, Tine Merete; Øvstebø, Reidun & Aass, Hans Christian
[Vis alle 8 forfattere av denne artikkelen](2023).
Isolation, characterization, and fibroblast uptake of bacterial extracellular vesicles from Porphyromonas gingivalis strains.
MicrobiologyOpen.
ISSN 2045-8827.
12(5).
doi: 10.1002/mbo3.1388.
Fulltekst i vitenarkiv
Lawrence, Graeme; Midtervoll, Ingvild K.; Samuelsen, Sven Ove; Kristoffersen, Anne Karin; Enersen, Morten & Lund Håheim, Lise
(2022).
The blood microbiome and its association to cardiovascular disease mortality: case-cohort study.
BMC Cardiovascular Disorders.
ISSN 1471-2261.
22(1).
doi: 10.1186/s12872-022-02791-7.
Fulltekst i vitenarkiv
Zandi, Homan; Petronijevic, Nikola; Mdala, Ibrahimu; Kristoffersen, Anne Karin; Enersen, Morten & Rocas, Isabela N.
[Vis alle 8 forfattere av denne artikkelen](2019).
Outcome of endodontic retreatment Using 2 root canal irrigants and influence of infection on healing as determined by a molecular method: A Randomized Clinical Trial.
Journal of Endodontics.
ISSN 0099-2399.
45(9),
s. 1089–1098.e5.
doi: 10.1016/j.joen.2019.05.021.
ABSTRACT
Objective: The aim of this clinical quality study was to determine whether the aseptic working field is
maintained during the endodontic procedure.
Materials and methods: Bacterial samples were collected from the rubber dam of 27 patients during
endodontic treatment performed by postgraduate students at the Department of Endodontics,
University of Oslo. A bacterial sample was first obtained immediately after disinfection of the working
field (A), and the second sample was collected just before obturation or dressing with calcium hydroxide
cement (B). Aerobic cultivation technique and PCR were used for detection of bacterial growth
and species.
Results: All samples were negative on culturing except in one case, which showed positive results
with cultivation in both sample A and B. Specie detected with cultivation technique were
Streptococcus mitis. With PCR technique, 6 samples in 5 patients (11%), showed positive results.
Species detected with PCR technique were Bacteroidales spp. Propionibacterium spp., Bacteroidetes spp.,
Prevotella nigrescens, Haemophilus parainfluenzae, Neisseria elongata, Alloprevotella tannerae,
Capnocytophaga granulosa, Cardiobacterium hominis, Fusobacterium nucleatum and Streptococcus mitis.
Conclusion: The present study showed that an aseptic working field was maintained throughout the
endodontic procedure in 81% (22/27) of the cases after disinfection of the rubber dam.
Valegård, Karin; Andralojc, P. John; Haslam, Richard P.; Pearce, F. Grant; Eriksen, Gunilla Kristina & Madgwick, Pippa J.
[Vis alle 12 forfattere av denne artikkelen](2018).
Structural and functional analyses of Rubisco from arctic diatom species reveal unusual posttranslational modifications.
Journal of Biological Chemistry.
ISSN 0021-9258.
293(34),
s. 13033–13043.
doi: 10.1074/jbc.RA118.003518.
Fulltekst i vitenarkiv
Olsen-Bergem, Heming; Kristoffersen, Anne Karin; Bjørnland, Tore & Aas, Jørn Arne
(2018).
Is it possible to contaminate the temporomandibular joint by arthrocentesis? Oral Surgery.
ISSN 1752-2471.
11(3),
s. 183–188.
doi: 10.1111/ors.12331.
Fulltekst i vitenarkiv
Zandi, Homan; Kristoffersen, Anne Karin; Ørstavik, Dag; Rocas, Isabela; Siqueira, Jose F. & Enersen, Morten
(2018).
Microbial Analysis of Endodontic Infections in Root-filled Teeth with Apical Periodontitis before and after Irrigation using Pyrosequencing.
Journal of Endodontics.
ISSN 0099-2399.
44(3),
s. 372–378.
doi: 10.1016/j.joen.2017.11.019.
Rodrigues, Renata Costa Val; Zandi, Homan; Kristoffersen, Anne Karin; Enersen, Morten; Mdala, Ibrahimu & Ørstavik, Dag
[Vis alle 8 forfattere av denne artikkelen](2017).
Influence of the apical preparation size and the irrigant type on bacterial reduction in root canal - treated teeth with apical periodontitis.
Journal of Endodontics.
ISSN 0099-2399.
43(7),
s. 1058–1063.
doi: 10.1016/j.joen.2017.02.004.
Larsen, Olav Inge; Enersen, Morten; Kristoffersen, Anne Karin; Wennerberg, Ann; Bunæs, Dagmar Fosså & Lie, Stein Atle
[Vis alle 7 forfattere av denne artikkelen](2017).
Antimicrobial effects of three different treatment modalities on dental implant surfaces.
Journal of Oral Implantology.
ISSN 0160-6972.
43(6),
s. 429–436.
doi: 10.1563/aaid-joi-D-16-00147.
Zandi, Homan; Rodrigues, Renata C.V.; Kristoffersen, Anne Karin; Enersen, Morten; Mdala, Ibrahimu & Ørstavik, Dag
[Vis alle 8 forfattere av denne artikkelen](2016).
Antibacterial Effectiveness of 2 Root Canal Irrigants in Root-filled Teeth with Infection: A Randomized Clinical Trial.
Journal of Endodontics.
ISSN 0099-2399.
42(9),
s. 1307–1313.
doi: 10.1016/j.joen.2016.06.006.
Langbach, Odd; Kristoffersen, Anne Karin; Abesha-Belay, Emnet; Enersen, Morten; Røkke, Ola & Olsen, Ingar
(2016).
Oral, intestinal, and skin bacteria in ventral hernia mesh implants.
Journal of Oral Microbiology.
ISSN 2000-2297.
8:31854(1),
s. 1–15.
doi: 10.3402/jom.v8.31854.
Fulltekst i vitenarkivVis sammendrag
Background: In ventral hernia surgery, mesh implants are used to reduce recurrence. Infection after mesh implantation can be a problem and rates around 6–10% have been reported. Bacterial colonization of mesh implants in patients without clinical signs of infection has not been thoroughly investigated. Molecular techniques have proven effective in demonstrating bacterial diversity in various environments and are able to identify bacteria on a gene-specific level.
Objective: The purpose of this study was to detect bacterial biofilm in mesh implants, analyze its bacterial diversity, and look for possible resemblance with bacterial biofilm from the periodontal pocket.
Methods: Thirty patients referred to our hospital for recurrence after former ventral hernia mesh repair, were examined for periodontitis in advance of new surgical hernia repair. Oral examination included periapical radiographs, periodontal probing, and subgingival plaque collection. A piece of mesh (1×1 cm) from the abdominal wall was harvested during the new surgical hernia repair and analyzed for bacteria by PCR and 16S rRNA gene sequencing. From patients with positive PCR mesh samples, subgingival plaque samples were analyzed with the same techniques.
Results: A great variety of taxa were detected in 20 (66.7%) mesh samples, including typical oral commensals and periodontopathogens, enterics, and skin bacteria. Mesh and periodontal bacteria were further analyzed for similarity in 16S rRNA gene sequences. In 17 sequences, the level of resemblance between mesh and subgingival bacterial colonization was 98–100% suggesting, but not proving, a transfer of oral bacteria to the mesh.
Conclusion: The results show great bacterial diversity on mesh implants from the anterior abdominal wall including oral commensals and periodontopathogens. Mesh can be reached by bacteria in several ways including hematogenous spread from an oral site. However, other sites such as gut and skin may also serve as sources for the mesh biofilm.
Andersen, Kari-Mette; Kristoffersen, Anne Karin; Ingebretsen, André; Vikholt, Katharina Johnsen; Ørtengren, Ulf Thore & Olsen, Ingar
[Vis alle 8 forfattere av denne artikkelen](2016).
Diversity and anti-fungal susceptibility of Norwegian Candida glabrata clinical isolates.
Journal of Oral Microbiology.
ISSN 2000-2297.
8(1).
doi: 10.3402/jom.v8.29849.
Fulltekst i vitenarkiv
Olsen-Bergem, Heming; Kristoffersen, Anne Karin; Bjørnland, Tore; Reseland, Janne Elin & Aas, Jørn Arne
(2016).
Juvenile idiopathic arthritis and rheumatoid arthritis: Bacterial diversity in temporomandibular joint synovial fluid in comparison with immunological and clinical findings.
International Journal of Oral & Maxillofacial Surgery.
ISSN 0901-5027.
45(3),
s. 318–322.
doi: 10.1016/j.ijom.2015.08.986.
Armingohar, Zahra; Jørgensen, Jørgen J.; Kristoffersen, Anne Karin; Schenck, Karl & Dembic, Zlatko
(2015).
Polymorphisms in the interleukin-10 gene and chronic periodontitis in patients with atherosclerotic and aortic aneurysmal vascular diseases.
Journal of Oral Microbiology.
ISSN 2000-2297.
7(1),
s. 1–8.
doi: 10.3402/jom.v7.26051.
Fulltekst i vitenarkiv
Armingohar, Zahra; Jørgensen, Jørgen J.; Kristoffersen, Anne Karin; Schenck, Karl & Dembic, Zlatko
(2014).
Polymorphisms in the interleukin-1 gene locus and chronic periodontitis in patients with atherosclerotic and aortic aneurysmal vascular diseases.
Scandinavian Journal of Immunology.
ISSN 0300-9475.
79(5),
s. 338–345.
doi: 10.1111/sji.12166.
Vis sammendrag
Chronic periodontitis (CP) and atherosclerotic and aortic aneurysmal vascular diseases (VD) are inflammatory conditions that share a number of predisposing factors. They have a complex genetic heritability and may share genetic risk factors, but a well-defined relationship is still not determined. In addition, distinct genetic patterns of predisposition have been associated with these diseases. Here, we investigated the association of polymorphisms in the IL-1 gene locus with CP in a case-case study analysing VD patients with or without CP. Seventy-four patients with VD of whom 36 had CP were genotyped for single nucleotide polymorphisms in the IL1A -889 (rs1800587), IL1B +3954 (rs1143634) and IL1B at -511 (rs16944) genes and for VNTR polymorphisms in the IL1RN gene. A significantly higher frequency (17%) for allele 1 (four repeats) of the IL1RN VNTR gene was found among the VD patients with CP compared to those without CP. In addition, the frequency of the IL1RN VNTR genotypes 1/1 (4/4 repeats) and 2/2 (2/2 repeats) were significantly higher and lower, respectively, in VD patients with CP. These findings suggest an association of genetic polymorphisms in the IL1-gene locus with risk for CP in patients with VD. The carriage of the risk genotypes, the development and the subsequent influence of CP on systemic health may constitute an additional burden in the pathogenesis of VD. This emphasizes the importance of effective periodontal treatment in patients with VD.
Armingohar, Zahra; Jørgensen, Jørgen J.; Kristoffersen, Anne Karin; Abesha-Belay, Emnet & Olsen, Ingar
(2014).
Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis.
Journal of Oral Microbiology.
ISSN 2000-2297.
6,
s. 1–13.
doi: 10.3402/jom.v6.23408.
Fulltekst i vitenarkiv
Kristoffersen, Anne Karin
(2011).
Laboratory methods for analyzing correlation between periodontitis and cardiovascular disease.
I Håheim, Anne-Lise Lund (Red.),
Oral infections and cardiovascular disease.
Bentham Science Publishers Ltd.
ISSN 978-1-60805-232-5.s. 65–77.
Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Vis alle 10 forfattere av denne artikkelen](2022).
Orale soppinfeksjoner - mer enn Candida albicans: mikrobiologiske analyser av prøver fra alvorlig syke pasienter med kreft .
Vis sammendrag
Bakgrunn
Pasienter med alvorlig kreftsykdom er utsatte for orale soppinfeksjoner, grunnet sykdommen, kreftbehandling og/eller svekket immunforsvar. Orale soppinfeksjon påvirker pasienters matlyst, ernæringstilstand og livskvalitet. Candida albicans er en vanlig soppart i munnhulen, men forekomst av non-albicans Candida-arter, og i kombinasjon med C. albicans, er økende. Artene har varierende toleranse for antimykotiske midler, det kan gi behandlingsutfordringer.
Hensikten med studien var å identifisere og resistensteste Candida sopparter i munnhulen til alvorlig syke pasienter med kreft.
Metode
Sopprøver tatt under studien Oral Health in Advanced Cancer i perioden 2014 – 2016, ble analysert høsten 2020. Pasientprøvene ble re-dyrket ved Institutt for oral biologi, Universitetet i Oslo. Soppartene ble identifisert biokjemisk og ved DNA sekvensering, og resistenstestet for antimykotiske legemidler: fluconazole, amphotericin B, anidulafungin og nystatin.
Resultat
Vi analyserte totalt 56 isolater fra 45 pasienter og identifiserte syv Candida arter og en Saccharomyces. Til sammen hadde 36 pasienter oppvekst av én soppart, og 9 pasienter hadde 2 til 3 ulike sopparter i munnhulen. Av C. albicans artene var 39 av 40 følsomme for fluconazole. To Candida parapsilosis og en Candida tropicalis var resistent mot anidulafungin. En C. tropicalis var resistent mot anidulafungin. En Candida glabrata var resistent mot fluconazole.
Konklusjon
Studien viste at forskjellige Candida arter forekommer både i monoinfeksjoner og blandingsinfeksjoner. C. albicans var dominerende med høy følsomhet for antimykotiske midler. Blandingsinfeksjoner kan komplisere behandlingen og forårsake gjentagende infeksjoner. Mikrobiologiske prøver og analyser med identifisering og resistenstesting, bør være en del av utredningen hos pasienter med alvorlig og tilbakevendende orale soppinfeksjoner.
Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Vis alle 10 forfattere av denne artikkelen](2022).
Oral candidiasis in advanced cancer – identification and susceptibility testing of detected Candida species.
Vis sammendrag
Introduction: Candida albicans is the most common fungal species in the oral cavity. Increased prevalence of non-C. albicans are detected, and co-infection with C. albicans may have implications for treatment, due to antifungal resistance. The aim of the study was to assess the diversity and susceptibility of oral yeasts in patients with advanced cancer.
Methods: Clinical fungal isolates were sampled in 2014-2016 from 45 patients in a hospice unit. Totally 56 isolates were grown on chromID Candida plates, prepared for biochemical identification, and controlled with DNA sequencing. E-test was performed with the antifungal agents fluconazole, amphotericin B, anidulafungin and nystatin were tested.
Results: A total of 56 isolates were identified. Candida species was dominated by C. albicans at 71%, followed by C. glabrata 9%, C. parapsilosis 5%, C. tropicalis 4%, C. krusei 2%, C. guilliermondii 2% and C. dubliniesis 2%. Nine patients had 2-3 different species. C. albicans strains were susceptible to fluconazole in 97.5%. One C. parapsilosis and one C. tropicalis strain were resistant to anidulafungin. One C. glabrata was resistant to fluconazole and another was just below the threshold for resistance. The sequencing confirmed the biochemical identification.
Conclusion: C. albicans was the most prevalent species. Variation in susceptibility was observed in patients with mixed colonies. Combinations of Candida species and the lack of identification and susceptibility testing may lead to inadequate treatment.
Monsen, Ragnhild Elisabeth; Kristoffersen, Anne Karin; Gay, Caryl; Herlofson, Bente Brokstad; Fjeld, Katrine Gahre & Hove, Lene Hystad
[Vis alle 10 forfattere av denne artikkelen](2022).
Identification and susceptibility testing of oral candidiasis in advanced cancer patients – an important diagnostic tool.
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Background: Candida albicans is the most common fungal species detected in the oral cavity. Studies of oral fungal samples have shown an increased prevalence of non-C. albicans species in mixed infection with C. albicans. Non-C. albicans and C. albicans are associated with varying degrees of resistance to azole. This may have implications for treatment, due to acquired antifungal resistance.
Aims: To assess the diversity and susceptibility of detected Candida species in the oral cavity in patients with advanced cancer.
Methods: Clinical fungal isolates sampled in 2014-2016 from 45 patients in a hospice unit were analyzed. A total of 56 isolates were grown on chromID Candida plates. After phenotypic identification by colour of the colonies, one single colony of each species was re-cultivated and prepared for identification on a VITEK2® system. Susceptibility testing with E-test was performed on RPMI agar, and the antifungal agents fluconazole, amphotericin B, anidulafungin and nystatin were tested.
Results: A total of 56 Candida isolates were identified. The diversity of Candida species was dominated by C. albicans at 89%, followed by C. glabrata 11%, C. parapsilosis 7%, C. tropicalis 4%, C. krusei 2%, C. guilliermondii 2% and C. dubliniesis 2%. Thirty-six patients had monoculture, and nine patients had 2-3 different species detected. Almost all C. albicans strains (97.5%) were susceptible to fluconazole. One C. parapsilosis and one C. tropicalis strain were resistant to anidulafungin. One C. glabrata was resistant to fluconazole and another was just below the threshold for resistance.
Conclusion: C. albicans was the most prevalent Candida species in our sample. Variation in susceptibility was observed in patients with both C. albicans and C. glabrata. Combinations of Candida species and the lack of identification and susceptibility testing may lead to inadequate treatment and accelerate the development of resistance.
Rogala, Agnieszka; Kristoffersen, Anne Karin; Enersen, Morten; Sapkota, Dipak & Küntziger, Thomas M.
(2021).
Defining the role of Porphyromonas gingivalis’ peptidyl arginine deiminase (PPAD) in oral carcinoma.
Rogala, Agnieszka; Follin-Arbelet, Benoit; Kristoffersen, Anne Karin; Enersen, Morten; Sapkota, Dipak & Küntziger, Thomas M.
(2019).
Defining the role of Porphyromonas gingivalis peptidyl arginine deiminase (PPAD) in oral carcinoma.
Rusthen, Shermin; Kristoffersen, Anne Karin; Young, Alix; Galtung, Hilde; Enersen, Morten & Jensen, Janicke Liaaen
(2018).
The Salivary Microbiota Profile in Reduced and Normal Salivation.
Langbach, Odd; Kristoffersen, Anne Karin; Abesha-Belay, Emnet; Enersen, Morten & Olsen, Ingar
(2016).
Nett i bukveggen hos brokkopererte inneholder orale mikrober.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
126(8),
s. 610–611.
Hallmer, Fredrik; Bjørnland, Tore; Kristoffersen, Anne Karin; Anderson, Gunilla & Enersen, Morten
(2015).
THE ASSOCIATION BETWEEN ORAL MICROFLORA AND BISPHOSPHONATE OR DENOSUMAB INDUCED OSTEONECROSIS - abstract
ICOMS 2015 - International Conference on Oral and Maxillofacial Surgery, Melbourne October 2015.
ICOMS 2015 - International Conference on Oral and Maxillofacial Surgery.
Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Vis alle 7 forfattere av denne artikkelen](2014).
Phenotypic characterization of Candida glabrata from blood, respiratory and gastrointestinal tracts.
Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Vis alle 7 forfattere av denne artikkelen](2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts.
Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Vis alle 7 forfattere av denne artikkelen](2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts. Poster presentation.
Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Vis alle 7 forfattere av denne artikkelen](2014).
Phenotypic Characterization of Candida glabrata from Blood, Respiratory and Gastrointestinal Tracts. Oral Presentation.
Midtervoll, Ingvild K.; Enersen, Morten; Kristoffersen, Anne Karin; Samuelsen, Sven Ove; Olsen, Ingar & Håheim, L.L.
(2014).
Detection of Periodontal Pathogens in Blood from Patients with Cardiovascular Disease. Poster presentation.
Midtervoll, Ingvild K.; Enersen, Morten; Kristoffersen, Anne Karin; Samuelsen, Sven Ove & Lund Håheim, Lise
(2014).
Detection of Periodontal Pathogens in Blood from Patients With Cardiovascular Disease. Oral Presentation.
Zandi, Homan; Rodrigues, Renata C. V.; Kristoffersen, Anne Karin; Ørstavik, Dag; Siqueira, Jose F. & Rocas, Isabela
[Vis alle 7 forfattere av denne artikkelen](2014).
Clinical effectiveness of two antimicrobial irrigants in endodontically treated teeth. Poster presentation.
Andersen, Kari-Mette; Enersen, Morten; Kristoffersen, Anne Karin; Ingebretsen, André; Ørtengren, Ulf Thore & Gaustad, Peter
[Vis alle 7 forfattere av denne artikkelen](2014).
Phenotypic diversity among clinical Candida glabrata strains in Norwegian health care unit. Oral presentation.
Zandi, Homan; Rodrigues, Renata C. V.; Kristoffersen, Anne Karin; Ørstavik, Dag; Siqueira, Jose F. & Rocas, Isabela
[Vis alle 7 forfattere av denne artikkelen](2014).
Clinical effectiveness of two antimicrobial irrigants in endodontically treated teeth. Oral presentation.
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Clinical Effectiveness of Two Antimicrobial Irrigants in Endodontically Treated Teeth
Homan Zandi, DDS, Renata C. V. Rodrigues, DDS, Anne-Karin Kristoffersen, PhD, Dag Ørstavik, DDS, PhD, José F. Siqueira Jr, DDS, PhD, Isabela Rôças, DDS, PhD, Morten Enersen, DDS, PhD
Abstract
Background: Endodontic treatment of root-filled teeth with post-treatment disease is especially challenging. The outcome of endodontic treatment is significantly influenced by the presence of bacteria in the root canals at the time of filling. Sodium hypochlorite (NaOCl) is widely used as a root canal irrigant in different concentrations because of its pronounced antimicrobial activity and the ability of dissolving necrotic tissue and organic matter. Chlorhexidine gluconate (CHX) is an effective substitute for NaOCl due to its broad-spectrum antimicrobial efficacy against bacteria and its high dentin substantivity. It is less toxic against vital tissues than NaOCl but lacks tissue dissolving property. No culture-independent molecular study has compared the antimicrobial effectiveness of 1% NaOCl and 2% CHX in root-filled teeth with apical periodontitis (AP). Objectives: The aim of the present study was to compare the antibacterial properties of 1% NaOCl and 2% CHX in endodontically treated teeth with AP. Methods: Sixty-eight root-filled teeth with AP were randomly distributed in two irrigant groups. Bacterial samples were taken at baseline (S1), after chemomechanical preparation using either 1% NaOCl or 2% CHX as irrigant (S2), and after intracanal medication with calcium hydroxide at the second visit (S3). The bacterial samples were analyzed by 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) for total bacteria, Enterococcus faecalis and Streptococcus spp.. Results: Preliminary results indicated bacterial DNA in all the samples at S1. E. faecalis was seldom found, but streptococci were frequent. The amount of bacterial DNA significantly declined following treatment in both groups (S2 and S3) (p<0.001). There was no significant difference between NaOCl and CHX groups in reducing total bacterial and streptococcal counts (p>0.05). Conclusion: Findings indicate that endodontic retreatment was equally effective in reducing the amount of bacteria in both irrigation groups.
Keywords:
Endodontics. Root canal irrigants.
Midtervoll, Ingvild K.; Kristoffersen, Anne Karin; Enersen, Morten; Samuelsen, Sven Ove & Lund Håheim, Lise
(2013).
PERIODONTAL PATHOGENS – A POSSIBLE ROLE IN CARDIOVASCULAR DISEASE? Vis sammendrag
Objective: The aim of this ongoing study is to investigate a possible role for periodontal pathogens in relation to cardiovascular disease (CVD) through detection of bacterial DNA in bloodsamples from participants of the Oslo II study.
Materials and methods: The study design is a blinded case-cohort design where the cohort is men from Oslo, born between 1926 and 1932. The test group consists of 225 individuals who have died from CVD (myocardial infarction, aortic aneurysms or stroke), and the control group are 225 healthy men randomly picked from the cohort. After DNA extraction from blood samples in both groups, PCR amplification of the 16S rRNA bacterial gene will be performed, and PCR products of 650bp fragment will be verified on a 1,5% agarose gel. Bacterial species will be detected by high throughput sequencing of the PCR products, and results from test and control group will be correlated. Furthermore, results from three diagnoses in the test group will also be correlated separately to the controls. Correction for known risk factors such as smoking, physical inactivity, high blood pressure, total serum cholesterol and diabetes will be performed.
Results: Preliminary results from PCR amplification of 260 blinded bloodsamples indicate bacterial DNA detection with the expected fragment size in 124 (48%) of the samples investigated so far.
Vikholt, Katharina Johnsen; Andersen, Kari-Mette; Kristoffersen, Anne Karin & Enersen, Morten
(2014).
Oral candidiasisand molecular epidemiology in Candida glabrata.
Universitetet i Oslo.
Solli, Silje Jeanette; Nguyen, Toan Duy; Kristoffersen, Anne Karin & Enersen, Morten
(2014).
RgpB-genet; en viktig virulensfaktor hos den periopatogene bakteriearten Porphyromonas gingivalis. Et molekylærbiologisk studium.
Universitetet i Oslo.