Cell culture in three dimensions
Krzysztof Wrzesinski from CelVivo gave us a detailed introduction to 3D cell culture methods
Our cell lab team was treated to a one-week crash course in the advantages and disadvatages of 3D cell culture as opposed to the traditional 2D well plate cell culture held by Krzysztof Wrzesinski from CelVivo. The theoretical course was accompanied by hands-on training on the practical and more technical details on the formation of 3D tissue mimetic cell constructs using a rotating bioreactor-clinostat from CelVivo. Krzysztof also gave a practical tutorial on histological preparation and imaging of the formed 3D tissue-like cell spheroids to our cell lab and histology team.
One of the CelVivo rotating bioreactors is currently running in our cell lab and is used to form 3D tissue-like bone spheres by co-culturing osteoblasts and osteoclasts. Such tissue mimetic cell constructs will be a useful tool for us to investigate biomolecular stimulation of tissue regeneration and the host tissue response towards biomaterials intended for tissue engineering applications.
Krzysztof was invited to visit our lab after meeting up with Professor Janne Reseland at a COST Action event focusing on 3D cell culture methods and their applications. Other activities within this programme has given two of our PhD students a chance to attend international training schools and workshops on 3D cell culture and bioprinting and an excellent opportunity to increase their own international research network.