Håvard Jostein Haugen

• Schröder, Maria Elisabeth; Reseland, Janne Elin & Haugen, Håvard Jostein (2022). Osteoblasts in a Perfusion Flow Bioreactor—Tissue Engineered Constructs of TiO2 Scaffolds and Cells for Improved Clinical Performance. Cells. ISSN 2073-4409. 11(13). doi: 10.3390/cells11131995. Fulltekst i vitenarkiv
• Hassan, Hafiz Wajahat; Rahmati, Maryam; Barrantes Bautista, Alejandro; Haugen, Håvard Jostein & Mirtaheri, Peyman (2022). In Vitro Monitoring of Magnesium-based Implants Degradation by Surface Analysis and Optical Spectroscopy". International Journal of Molecular Sciences. ISSN 1661-6596. 23(11). doi: 10.3390/ijms23116099. Fulltekst i vitenarkiv Vis sammendrag

  Magnesium (Mg)-based degradable alloys have attracted substantial attention for tissue engineering applications due to their biodegradability and potential for avoiding secondary removal surgeries. However, insufficient data in the existing literature regarding Mg’s corrosion and gas formation after implantation have delayed its wide clinical application. Since the surface properties of degradable materials constantly change after contact with body fluid, monitoring the behavior of Mg in phantoms or buffer solutions could provide some information about its physicochemical surface changes over time. Through surface analysis and spectroscopic analysis, we aimed to investigate the structural and functional properties of degradable disks. Since bubble formation may lead to inflammation and change pH, monitoring components related to acidosis near the cells is essential. To study the bubble formation in cell culture media, we used a newly developed Mg alloy (based on Mg, Zinc and Calcium), pure Mg and commercially available grade 2 Titanium (Ti) disks in Dulbecco's Modified Eagle Medium (DMEM) solution to observe their behavior over ten days of immersion. Using surface analysis, and the information from near-infrared spectroscopy (NIRS), we concluded conditions associated with the medical risks of Mg alloy disintegration. NIRS is used to investigate the degradation behaviour of Mg-based disks in the cell culture media, which is correlated with the surface analysis where possible.

• D. Tien, Nguyen; Geng, Tianxiang; Heyward, Catherine Anne; Reseland, Janne Elin; Lyngstadaas, Ståle Petter & Blaker, Jonathan James [Vis alle 7 forfattere av denne artikkelen] (2022). Solution blow spinning of highly deacetylated chitosan nanofiber scaffolds for dermal wound healing. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. doi: 10.1016/j.bioadv.2022.212871. Fulltekst i vitenarkiv
• Kallas, Pawel; Valen, Håkon; Hulander, Mats; Gadegaard, Nikolaj; Stormonth-Darling, John & O'Reilly, Padraic [Vis alle 9 forfattere av denne artikkelen] (2022). Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli. Nanoscale. ISSN 2040-3364. doi: 10.1039/D2NR00976E. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein & Chen, Hongyu (2022). Is There a Better Biomaterial for Dental Implants than Titanium?—A Review and Meta-Study Analysis. Journal of Functional Biomaterials. ISSN 2079-4983. 13(2). doi: 10.3390/jfb13020046. Fulltekst i vitenarkiv
• Bjørge, Isabel M.; de Sousa, Barbara M; Patricio, Sonia; Silva, Ana Sofia; Parreiras Nogueira, Liebert & Santos, Lucia [Vis alle 10 forfattere av denne artikkelen] (2022). Bioengineered hierarchical bone-like compartmentalized microconstructs using nanogrooved microdiscs. ACS Applied Materials & Interfaces. ISSN 1944-8244.
• Luzon, Javier; Thorsen, Yngve; Parreiras Nogueira, Liebert; Andersen, Solveig Norheim; Edwin, Bjørn von Gohren & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2022). Reconstructing topography and extent of injury to the superior mesenteric artery plexus in right colectomy with extended D3 mesenterectomy: a composite multimodal 3-dimensional analysis. Surgical Endoscopy. ISSN 0930-2794. doi: 10.1007/s00464-022-09200-2. Fulltekst i vitenarkiv
• Johnsen, Gaute Floer; Haugen, Håvard Jostein; Parreiras Nogueira, Liebert; Sevgi, Usame; Jimenez, Ashley Mae & DeLuca, Joseph T. [Vis alle 8 forfattere av denne artikkelen] (2022). Assessment of Root Canal Similarity in Contralateral Mandibular Incisors. International Dental Journal. ISSN 0020-6539. doi: 10.1016/j.identj.2022.04.003. Fulltekst i vitenarkiv
• Øvrebø, Øystein; Perale, Giuseppe; Wojciechowski, Jonathan P.; Echalier, Cécile Lucie; Jeffers, Jonathan R. T. & Stevens, Molly M. [Vis alle 8 forfattere av denne artikkelen] (2022). Design and clinical application of injectable hydrogels for musculoskeletal therapy. Bioengineering & Translational Medicine. ISSN 2380-6761. doi: 10.1002/btm2.10295. Fulltekst i vitenarkiv Vis sammendrag

  Musculoskeletal defects are an enormous healthcare burden and source of pain and disability for individuals. With an ageing population, the proportion living with these medical indications will increase. Simultaneously, there is pressure on healthcare providers to source efficient solutions, which are cheaper and less invasive than conventional technology. This has led to an increased research focus on hydrogels as highly biocompatible biomaterials that can be delivered through minimally invasive procedures. This review will discuss how hydrogels can be designed for clinical translation, particularly in the context of the new European Medical Device Regulation (MDR). We will then do a deep dive into the clinically used hydrogel solutions that have been commercially approved or have undergone clinical trials in Europe or the US. We will discuss the therapeutic mechanism and limitations of these products. Due to the vast application areas of hydrogels, this work focuses only on treatments of cartilage, bone, and the nucleus pulposus. Lastly, the main steps towards clinical translation of hydrogels as medical devices are outlined. We suggest a framework for how academics can assist small and medium MedTech enterprises conducting the initial clinical investigation and Post-Market Clinical Follow-up (PMCF) required in the MDR. It is evident that the successful translation of hydrogels is governed by acquiring high-quality pre-clinical and clinical data confirming the device mechanism of action and safety.

• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Mao, Chenyi; Ali, Adilijiang & Vaughan, Joshua C. [Vis alle 14 forfattere av denne artikkelen] (2021). Intrinsically disordered peptides enhance regenerative capacities of bone composite xenografts . Materials Today. ISSN 1369-7021. 52, s. 63–79. doi: 10.1016/j.mattod.2021.12.001. Fulltekst i vitenarkiv Vis sammendrag

  Biomaterial scientists design organic bone substitutes based on the biochemical properties of the mimicked tissue to achieve near native functionality. Several non-collagenous proteins in bone are known as intrinsically disordered proteins (IDPs), as they lack detectible ordered domains and a fixed 3D structure under physiological conditions. Many IDPs perform regulatory roles in a range of cellular functions, which motivated us to design two proline-rich disordered peptides (P2 and P6) and augmented them into the SmartBone® (SBN) biohybrid substitute. Recently we reported an improved proliferation and osteogensis of human osteoblasts and mesenchymal stem cells in the composite groups containing peptides (named here as SBN + P2 and SBN + P6) in vitro. To address the effects of these composites on bone formation and biomineralization, this in vivo study investigated their functions in critical size craniotomy defects in 16 domestic pigs after 8 and 16 weeks of healing. For this purpose, we used cone beam computed tomography (CBCT), microCT (µCT), histology, immunohistochemistry, fluorescent labeling of abundant reactive entities (FLARE), synchrotron SAXS/XRD, optical photothermal IR (O-PTIR) microscopy and nanoscale atomic force microscopy-infrared (AFM-IR) analyses. Our results represent new synthetic IDPs as potential candidates for directing bone formation and biomineralization. The SBN + P6 stimulated significantly higher bone formation and biomineralization after 8 weeks of healing compared to other groups indicating its potential in stimulating early biomineralization. After 16 weeks of healing, the SBN + P2 induced significantly higher bone formation and biomineralization compared to other groups indicating its effects on later bone formation and biomineralization processes. The vigorous stretching of amide primary and secondary IR absorbance peaks at 1660 and 1546 cm−1 in the SBN + P2 group verified that this peptide experienced more conformational changes after 16 weeks of implantation with a higher phosphate intensity at 1037 cm−1 compared to peptide 6. Overall, P2 and P6 are promising candidates for bone augmentation strategies in critical clinical applications. We concluded that FLARE and O-PTIR are promising tools in evaluating and diagnosing the biochemical structure of bone tissue and the bone-biomaterial interface.

• Wang, Zhule; Parreiras Nogueira, Liebert; Haugen, Håvard Jostein; Van Der Geest, Ingrid C M; Caetano de Almeida Ro, Patricia & Janssen, Dennis [Vis alle 9 forfattere av denne artikkelen] (2021). Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells. Bioactive Materials. ISSN 2452-199X. doi: 10.1016/j.bioactmat.2021.12.023. Fulltekst i vitenarkiv
• Bakkalci, Deniz; Jay, Amrita; Rezaei, Azadeh; Howard, Christopher A.; Haugen, Håvard Jostein & Pape, Judith [Vis alle 12 forfattere av denne artikkelen] (2021). Bioengineering the ameloblastoma tumour to study its efect on bone nodule formation. Scientific Reports. ISSN 2045-2322. 11(24088 ). doi: 10.1038/s41598-021-03484-5. Fulltekst i vitenarkiv Vis sammendrag

  Ameloblastoma is a benign, epithelial cancer of the jawbone, which causes bone resorption and disfigurement to patients affected. The interaction of ameloblastoma with its tumour stroma drives invasion and progression. We used stiff collagen matrices to engineer active bone forming stroma, to probe the interaction of ameloblastoma with its native tumour bone microenvironment. This bone-stroma was assessed by nano-CT, transmission electron microscopy (TEM), Raman spectroscopy and gene analysis. Furthermore, we investigated gene correlation between bone forming 3D bone stroma and ameloblastoma introduced 3D bone stroma. Ameloblastoma cells increased expression of MMP-2 and -9 and RANK temporally in 3D compared to 2D. Our 3D biomimetic model formed bone nodules of an average surface area of 0.1 mm2 and average height of 92.37 ± 7.96 μm over 21 days. We demonstrate a woven bone phenotype with distinct mineral and matrix components and increased expression of bone formation genes in our engineered bone. Introducing ameloblastoma to the bone stroma, completely inhibited bone formation, in a spatially specific manner. Multivariate gene analysis showed that ameloblastoma cells downregulate bone formation genes such as RUNX2. Through the development of a comprehensive bone stroma, we show that an ameloblastoma tumour mass prevents osteoblasts from forming new bone nodules and severely restricted the growth of existing bone nodules. We have identified potential pathways for this inhibition. More critically, we present novel findings on the interaction of stromal osteoblasts with ameloblastoma.

• Thieu, Minh Khai Le; Homayouni, Amin; Hæren, Lena Ringsby; Tiainen, Hanna; Verket, Anders & Ellingsen, Jan Eirik [Vis alle 13 forfattere av denne artikkelen] (2021). Impact of simultaneous placement of implant and block bone graft substitute: an in vivo peri-implant defect model. Biomaterials Research. ISSN 1226-4601. 25(43). doi: 10.1186/s40824-021-00245-3. Fulltekst i vitenarkiv
• Hussain, Badra; Karaca, Ebru Ozkan; Kuru, Bahar Eren; Gursoy, Hare; Haugen, Håvard Jostein & Wohlfahrt, Johan Caspar (2021). Treatment of residual pockets using an oscillating chitosan device versus regular curettes alone—a randomized, feasibility parallel-arm clinical trial. Journal of Periodontology. ISSN 0022-3492. doi: 10.1002/JPER.21-0496. Fulltekst i vitenarkiv
• Hussain, Badra; Haugen, Håvard Jostein; Aass, Anne Merete; Sanz Alonso, Mariano; Antonoglou, Georgios & Bouchard, Philippe [Vis alle 23 forfattere av denne artikkelen] (2021). Peri-Implant Health and the Knowing-Doing Gap—A Digital Survey on Procedures and Therapies. Frontiers in Dental Medicine. ISSN 2673-4915. 2(726607). doi: 10.3389/fdmed.2021.726607. Fulltekst i vitenarkiv
• Rahmati, Maryam; Stötzel, Sabine; El Khassawna, Thaqif; Iskhahova, Kamila; Wieland, D.C. Florian & Plumhoff, Berit Zeller [Vis alle 7 forfattere av denne artikkelen] (2021). Early Osteoimmunomodulatory Effects of Magnesium–Calcium–Zinc alloys. Journal of Tissue Engineering. ISSN 2041-7314. doi: 10.1177/20417314211047100. Fulltekst i vitenarkiv Vis sammendrag

  Today, substantial attention is given to biomaterial strategies for bone regeneration, and among them, there is a growing interest in using immunomodulatory biomaterials. The ability of a biomaterial to induce neo vascularization and macrophage polarization is a major factor in defining its success. Magnesium (Mg)-based degradable alloys have attracted significant attention for bone regeneration owing to their biodegradability and potential for avoiding secondary removal surgeries. However, there is insufficient evidence in the literature regarding the early inflammatory responses to these alloys in vivo. In this study, we investigated the early body responses to Mg 0.45 wt%Zn-0.45 wt%Ca pin-shaped alloy (known as ZX00 alloy) in rat femora 2, 5 and 10 days after implantation. We used 3D micro computed tomography (μCT), histological, immunohistochemical, histomorphometrical and small angle X-ray scattering (SAXS) analyses to study new bone formation, early macrophage polarization, neo vascularization and bone quality at the implant bone interface. The expression of macrophage type 2 biological markers increased significantly after 10 days of Mg alloy implantation, indicating its potential in stimulating macrophage polarization. Our biomineralization results using μCT as well as histological stained sections did not indicate any statistically significant differences between different time points for both groups. The activity of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (Runx 2) biological markers decreased significantly for Mg group, indicating less osteoblast activity. Generally, our results supported the potential of ZX00 alloy to enhance the expression of macrophage polarization in vivo; however, we could not observe any statistically significant changes regarding biomineralization.

• Palombo, David; Rahmati, Maryam; Vignoletti, Fabio; Esporrin, Javier Sanz; Haugen, Håvard Jostein & Sanz, Mariano (2021). Hard and soft tissue healing around implants with a modified implant neck configuration: an experimental in vivo preclinical investigation. Clinical Oral Implants Research. ISSN 0905-7161. doi: 10.1111/clr.13812. Fulltekst i vitenarkiv
• Marovic, Danijela; Haugen, Håvard Jostein; Mandic, Visnja Negovetic; Par, Matej; Zheng, Kai & Tarle, Zrinka [Vis alle 7 forfattere av denne artikkelen] (2021). Incorporation of Copper-Doped Mesoporous Bioactive Glass Nanospheres in Experimental Dental Composites: Chemical and Mechanical Characterization. Materials. ISSN 1996-1944. 14(2611), s. 1–12. doi: 10.3390/ma14102611. Fulltekst i vitenarkiv
• D. Tien, Nguyen; Lyngstadaas, Ståle Petter; Mano, Joao F.; Blaker, Jonathan James & Haugen, Håvard Jostein (2021). Recent Developments in Chitosan-Based Micro/Nanofibers for Sustainable Food Packaging, Smart Textiles, Cosmeceuticals, and Biomedical Applications. Molecules. ISSN 1420-3049. 26(2683), s. 1–24. doi: 10.3390/molecules26092683. Fulltekst i vitenarkiv
• Torben, Hildebrand; Nogueira, Liebert Parreiras; Sunde, Pia Titterud; Ørstavik, Dag; Glasmacher, Birgit & Haugen, Håvard Jostein (2021). Contrast-enhanced nano-CT reveals dental soft tissues and cellular layers. International Endodontic Journal. ISSN 0143-2885. doi: 10.1111/iej.13527. Fulltekst i vitenarkiv
• Zhu, Hao; Haugen, Håvard Jostein; Perale, Giuseppe; Reseland, Janne Elin; Nogueira, Liebert Parreiras & Cantalapiedra, Antonio gonzalez [Vis alle 11 forfattere av denne artikkelen] (2021). Tailoring Resorption Rates and Osteogenic Response in Xeno-Hybrid Bone Grafts: The Effect of Added Gelatins. Engineering (Beijing). ISSN 2095-8099. doi: 10.1016/j.eng.2021.01.010. Fulltekst i vitenarkiv
• Thieu, Minh Khai Le; Haugen, Håvard Jostein; Esporrin, Javier Sanz; Sanz, Mariano; Lyngstadaas, Ståle Petter & Verket, Anders (2021). Guided bone regeneration of chronic non-contained bone defects using a volume stable porous block TiO2 scaffold: An experimental in vivo study. Clinical Oral Implants Research. ISSN 0905-7161. doi: 10.1111/clr.13708.
• Di Raimondo, Ricardo; Sanz, Javier; Sanz Martin1, Ignacio; Vignoletti, Fabio; Nuñez, Javi & Muñoz, Fernando [Vis alle 8 forfattere av denne artikkelen] (2021). Hard tissue volumetric and soft tissue contour linear changes at implants with different surface characteristics after experimentally induced peri-implantitis: an experimental in vivo investigation. Clinical Oral Investigations. ISSN 1432-6981. doi: 10.1007/s00784-020-03720-8. Vis sammendrag

  Objective To evaluate the hard tissue volumetric and soft tissue contour linear changes in implants with two different implant surface characteristics after a ligature-induced peri-implantitis. Material andmethods In eight beagle dogs, implants with the same size and diameter but distinct surface characteristics were placed in the healed mandibular sites. Test implants had an external monolayer of multi-phosphonate molecules (B+), while control implants were identical but without the phosphonate-rich surface. Once the implants were osseointegrated, oral hygiene was interrupted and peri-implantitis was induced by placing subgingival ligatures. After 16 weeks, the ligatures were removed and peri-implantitis progressed spontaneously. Bone to implant contact (BIC) and bone loss (BL) were assessed three-dimensionally with Micro-Ct (μCT). Dental casts were optically scanned and the obtained digitalized standard tessellation language (STL) images were used to assess the soft tissue vertical and horizontal contour linear changes. Results Reduction of the three-dimensional BIC percentage during the induction and progression phases of the experimental peri-implantitis was similar for both the experimental and control implants, without statistically significant differences between them. Soft tissue analysis revealed for both implant groups an increase in horizontal dimension after the induction of peri-implantitis, followed by a decrease after the spontaneous progression period. In the vertical dimension, a soft tissue dehiscence was observed in both groups, being more pronounced at the buccal aspect. Conclusions The added phosphonate-rich surface did not provide a more resistant environment against experimental periimplantitis, when assessed by the changes in bone volume and soft tissue contours. Clinical relevance Ligature-induced peri-implantitis is a validated model to study the tissue changes occurring during peri-implantitis. It was hypothesized that a stronger osseointegration mediated by the chemical bond of a phosphonaterich implant surface would develop an environment more resistant to the inflammatory changes occurring after experimental peri-implantitis. These results, however, indicate that the hard and soft tissue destructive changes occurring at both the induction and progression phases of experimental peri-implantitis were not influenced by the quality of osseointegration.

• Vyas, Cian; Zhang, Jun; Øvrebø, Øystein; Huang, Boyang; Roberts, Iwan & Setty, Mohan [Vis alle 10 forfattere av denne artikkelen] (2021). 3D printing of silk microparticle reinforced polycaprolactone scaffolds for tissue engineering applications. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 118. doi: 10.1016/j.msec.2020.111433. Fulltekst i vitenarkiv
• Sukul, Mousumi; Sahariah, Priyanka; Lauzon, Hélène L.; Borges, João; Másson, Már & Mano, Joao F. [Vis alle 8 forfattere av denne artikkelen] (2021). In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight. Carbohydrate Polymers. ISSN 0144-8617. 254. doi: 10.1016/j.carbpol.2020.117434. Fulltekst i vitenarkiv
• Zhu, Hao; Blahnová, Veronika Hefka; Perale, Giuseppe; Xiao, Jun; Betge, Felice & Boniolo, Fabio [Vis alle 9 forfattere av denne artikkelen] (2020). Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs. Frontiers in Cell and Developmental Biology. ISSN 2296-634X. doi: 10.3389/fcell.2020.619111. Fulltekst i vitenarkiv
• Kallas, Pawel; Haugen, Håvard Jostein; Gadegaard, Nikolaj; Stormonth-Darling, John; Hulander, Mats & Andersson, Martin [Vis alle 7 forfattere av denne artikkelen] (2020). Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae. Nanomaterials. ISSN 2079-4991. 10(11). doi: 10.3390/nano10112247. Fulltekst i vitenarkiv
• Gross, Karlis Agris; Petzold, Christiane; Pluduma, Liene; Kumermanis, Maris & Haugen, Håvard Jostein (2020). Structural and Chemical Hierarchy in Hydroxyapatite Coatings . Materials. ISSN 1996-1944. 13(19), s. 1–15. doi: 10.3390/ma13194447. Fulltekst i vitenarkiv Vis sammendrag

  Hydroxyapatite coatings need similarly shaped splats as building blocks and then a homogeneous microstructure to unravel the structural and chemical hierarchy for more refined improvements to implant surfaces. Coatings were thermally sprayed with differently sized powders (20-40, 40-63 and 63-80 µm) to produce flattened homogeneous splats. The surface was characterized for splat shape by profilometry and AFM, crystal size by AFM, crystal orientation by X-ray diffraction (XRD) and structural variations by XRD. Chemical composition was assessed by phase analysis, but variations in chemistry were detected by XRD and Raman spectroscopy. The resulting surface electrical potential was measured by Kelvin probe AFM. Five levels of structural hierarchy were suggested: the coating, the splat, oriented crystals, alternate layers of oxyapatite and HAp, and the suggested anion orientation. Chemical hierarchy was present over a lower range of order for smaller splats. Coatings made from smaller splats exhibited a greater electrical potential, inferred to arise from oxyapatite, and supplemented by ordered OH- ions in a rehydroxylated surface layer. A model has been proposed to show the influence of structural hierarchy on the electrical surface potential. Structural hierarchy is proposed as a means to further refine the properties of implant surfaces.

• Haugen, Håvard Jostein; Qasim, Syed Saad Bin; Matinlinna, Jukka P; Vallittu, Pekka K. & Nogueira, Liebert Parreiras (2020). Nano-CT as tool for characterization of dental resin composites. Scientific Reports. ISSN 2045-2322. 10. doi: 10.1038/s41598-020-72599-y. Fulltekst i vitenarkiv Vis sammendrag

  Technological advances have made it possible to examine dental resin composites using 3D nanometer resolution. This investigation aims to characterize existing dental nano-hybrid and micro-hybrid resin composites through comparing and contrasting nano-computed tomography (nano-CT) with micro-CT and high-resolution SEM images. Eight commercially available and widely used dental resin composites, 2 micro-hybrid and 6 nano-hybrid were researched. Cured samples were examined and characterized using nano-CT (resolution 450 nm) and compared with micro-CT images (resolution 2 µm). Acquired images were reconstructed and image analysis was carried out to determine porosity and pore morphology. A comprehensive comparison of scanning micrograph images unsurprisingly revealed that the nano-CT images displayed greater detail of the ultrastructure of cured dental resin composites. Filler particle diameters and its volumes were lower when measured using nano-CT, porosity being higher where analysed at higher resolution. There were large variations between the examined materials. Fewer voids were found in Tetric EvoCeram and IPS Empress Direct, the smallest pores being found in Universal XTE and Tetric EvoCeram. Nano-CT was successfully used to investigate the morphology of dental resin composites and showed that micro-CT gives a lower porosity and pore size but overestimates filler particle size. There were large discrepancies between the tested composites. Evidence of porosities and pores within a specimen is a critical finding and it might have a detrimental effect on a material’s clinical performance.

• Kallas, Pawel; Kang, Hua; Valen, Håkon; Haugen, Håvard Jostein; Andersson, Martin & Hulander, Mats (2020). Effect of silica nano-spheres on adhesion of oral bacteria and human fibroblasts. Biomaterial Investigations in Dentistry. 7(1), s. 134–145. doi: 10.1080/26415275.2020.1816175. Fulltekst i vitenarkiv
• Borie, Manon; Lecloux, Geoffrey; Bosshardt, Dieter D.; Barrantes Bautista, Alejandro; Haugen, Håvard Jostein & Lambert, France [Vis alle 7 forfattere av denne artikkelen] (2020). Peri-Implant Soft Tissue Integration in Humans – Influence of Materials: A study protocol for a randomised controlled trial and a pilot study results. Contemporary Clinical Trials Communications. ISSN 2451-8654. doi: 10.1016/j.conctc.2020.100643. Fulltekst i vitenarkiv Vis sammendrag

  Background : Recently, there has been a growing interest in mucointegration as the formation of an early and long-standing soft tissue barrier seems essential for both the initial healing and long-term implant survival. Aim: To develop an experimental method to characterize the mucointegration of different transgingival materials (titanium (Ti), polyetheretherketone (PEEK), polymethylmethacrylate (PMMA), zirconia (Zi), polymer infiltrated ceramic network (PICN), cobalt-chrome (Co-Cr), and lithium disilicate (LD)) in a human model. Methods / Design : The study is designed as a multi-part randomized controlled clinical trial. Ninety bone level Straumann implants will randomly receive an experimental, custom-made abutment to allow for the removal of the abutment together with the surrounding soft tissues using a punch biopsy device at 8 weeks of healing (10 per material). The specimens will be further processed for non‐decalcified histology, followed by histomorphometric analysis. The same protocol will be used for additional 90 implants-abutments, but during harvesting, soft tissues will be separated from the abutment and processed for immunohistochemistry in order to study tissue inflammation and vascularization, while the abutments will undergo SEM analysis. Additionally, in vitro analyses, including SEM and profilometry, will be performed in order to characterize surface topography of all experimental materials. Conclusion : The limited number of pilot samples presented herein indicate that the use of custommade abutments in humans is a reproducible method to study peri-implant soft tissue integration. This further intensifies the rationale to compare different abutment materials, used as transgingival components in daily practice, under the same conditions.

• Mengjun, Xue; Sampath, Janani; Gebart, Rachel; Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter & Pfaendtner, Jim [Vis alle 7 forfattere av denne artikkelen] (2020). Studies of dynamic binding of amino acids to TiO2 nanoparticle surfaces by Solution NMR and Molecular Dynamics Simulations. Langmuir. ISSN 0743-7463. doi: 10.1021/acs.langmuir.0c01256. Fulltekst i vitenarkiv Vis sammendrag

  Adsorption of biomolecules onto material surfaces involves a potentially complex mechanism where molecular species interact to varying degrees with a heterogeneous material surface. Surface adsorption studies by atomic force microscopy (AFM), Sum Frequency Generation (SFG) spectroscopy, and solid state NMR (ssNMR), detect the structures and interactions of biomolecular species that are bound to material surfaces and which, in the absence of a solid liquid interface, do not exchange rapidly between surface-bound forms and free molecular species in bulk solution. Solution NMR has the potential to complement these techniques by detecting and studying transiently bound biomolecules at the liquid-solid interface. Herein we show that dark-state exchange saturation transfer (DEST) NMR experiments on gel-stabilized TiO2 nanoparticle (NP) samples detect several forms of biomolecular adsorption onto titanium (IV) oxide surfaces. Specifically, we use the DEST approach to study the interaction of amino acids arginine (Arg), lysine (Lys), leucine (Leu), alanine (Ala), and aspartic acid (Asp) with TiO2 rutile nanoparticle surfaces. Whereas Leu, Ala, and Asp display only a single weakly interacting form in the presence of TiO2 nanoparticles , Arg and Lys displayed at least two distinct bound forms: a species that is surface bound and retains a degree of reorientational motion, and a second more tightly bound form characterized by broadened DEST profiles upon addition of TiO2 nanoparticles. Molecular Dynamics simulations indicate different surface bound states for both Lys and Arg depending on the degree of TiO2 surface hydroxylation, but only a single bound state for Asp regardless of the degree of surface hydroxylation, in agreement with results obtained from analysis of DEST profiles.

• Haugen, Håvard Jostein; Marovic, Danijela; Par, Matej; Thieu, Minh Khai Le; Reseland, Janne Elin & Johnsen, Gaute Floer (2020). Bulk Fill Composites Have Similar Performance to Conventional Dental Composites. International Journal of Molecular Sciences. ISSN 1661-6596. 21(15). doi: 10.3390/ijms21145136. Fulltekst i vitenarkiv Vis sammendrag

  The aim of the study was to perform comprehensive characterization of two commonly used bulk fill composite materials (SDR Flow (SDR) and Filtek™ Bulk Fill Flowable Restorative (FBF) and one conventional composite material (Tetric EvoCeram; TEC). Eleven parameters were examined: flexural strength (FS), flexural modulus (FM), degree of conversion, depth of cure, polymerisation shrinkage (PS), filler particle morphology, filler mass fraction, Vickers hardness, surface roughness following simulated toothbrush abrasion, monomer elution, and cytotoxic reaction of human gingival fibroblasts, osteoblasts, and cancer cells. The degree of conversion and depth of cure were the highest for SDR, followed by FBF and TEC, but there was no difference in PS between them. FS was higher for bulk fill materials, while their FM and hardness were lower than those of TEC. Surface roughness decreased in the order TEC→SDR→FBF. Bisphenol A-glycidyl methacrylate (BisGMA) and urethane dimethacrylate were found in TEC and FBF eluates, while SDR released BisGMA and triethylene glycol dimethacrylate. Conditioned media accumulated for 24h from FBF and TEC were cytotoxic to primary human osteoblasts. Compared to the conventional composite, the tested bulk fill materials performed equally or better in most of the tests, except for their hardness, elastic modulus, and biocompatibility with osteoblasts.

• Rahmati, Maryam; Frank, Matthias Johannes; Walter, Martin Sebastian; Monjo Cabrer, Marta; Satué, María & Reseland, Janne Elin [Vis alle 8 forfattere av denne artikkelen] (2020). Osteoimmunomodulatory effects of enamel matrix derivate and strontium coating layers: A short- and long-term in vivo study. ACS Applied Bio Materials (AABM). ISSN 2576-6422. 3, s. 5169–5181. doi: 10.1021/acsabm.0c00608. Fulltekst i vitenarkiv
• Rahmati, Maryam; Silva, Eduardo A.; Reseland, Janne Elin; Heyward, Catherine Anne & Haugen, Håvard Jostein (2020). Biological responses to physicochemical properties of biomaterial surface. Chemical Society Reviews. ISSN 0306-0012. 49(15), s. 5178–5224. doi: 10.1039/d0cs00103a. Fulltekst i vitenarkiv
• Rahmati, Maryam; Lyngstadaas, Ståle Petter; Reseland, Janne Elin; Andersbakken, Ingrid; Haugland, Heidi Straume & López-peña, Mónica [Vis alle 9 forfattere av denne artikkelen] (2020). Coating doxycycline on titanium-based implants: two in vivo studies. Bioactive Materials. ISSN 2452-199X. 5(4), s. 787–797. doi: 10.1016/j.bioactmat.2020.05.007. Fulltekst i vitenarkiv Vis sammendrag

  Regardless of the substantial progress in designing titanium-based dental implants and aseptic techniques, infection remains as the most common complication after implantation surgeries. Although, having a weakened immune system or systematic diseases is not seen as contraindicated for dental implants anymore, controlling the immune system is required to avoid surgical site infections after implantation. These patients have to control the surgical site infections by taking a high daily dose of oral antibiotics after dental implantation. The antibiotics oral administration has many side effects such as gastrointestinal symptoms, skin rashes and thrush. Coating antibiotics on the biomaterials surface could be a promising solution to reduce these disadvantages through locally releasing antibiotics in a controlled manner. The aim of this study was to investigate the effects of doxycycline coating layer on titanium-zirconium alloy surfaces in vitro and in vivo. In our previous studies, we demonstrated the chemical presence of doxycycline layer in vitro. In this study, we examined its physical presence using field emission scanning electron microscope and confocal microscope. We also analyzed its controlled released manner using Nano-Drop UV Vis spectrometer. After in vitro characterization of the coating layer, we evaluated its effects on the implant osseointegration in dogs and rabbits. The histological and histomorphometrical results exhibited no significant difference between doxycycline coated and uncoated groups regarding the implants osseointegration and biocompatibility for dental applications. Therefore, coating a doxycycline layer on TiZr implants could be favorable for reducing or removing the antibiotics oral administration after the implantation surgery.

• Chahal, Aman S; Schweikle, Manuel; Lian, Aina-Mari; Reseland, Janne Elin; Haugen, Håvard Jostein & Tiainen, Hanna (2020). Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells. Journal of Tissue Engineering. ISSN 2041-7314. 11. doi: 10.1177/2041731420926840. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein; Basu, Poulami; Sukul, Mousumi; Mano, Joao F. & Reseland, Janne Elin (2020). Injectable biomaterials for Dental Tissue Regeneration. International Journal of Molecular Sciences. ISSN 1661-6596. 21. doi: 10.3390/ijms21103442. Fulltekst i vitenarkiv Vis sammendrag

  Injectable biomaterials scaffolds play a pivotal role for dental tissue regeneration, as such materials are more applicable in the dental field, particularly when compared to pre-formed scaffolds. The defects in maxilla-oral area are normally small, confined and sometimes hard to access. This narrative review described different types of biomaterials for dental tissue regeneration, also discussing the potential use of nanofibres for dental tissues. Various studies suggest that the tissue engineering approaches involving the use of injectable biomaterials have the potential of restoring not only dental tissue function but also their biological purposes

• Sukul, Mousumi; Cama, Giuseppe; Dubruel, Peter; Reseland, Janne Elin & Haugen, Håvard Jostein (2020). Methacrylation increase growth and differentiation of primary human osteoblasts for gelatin hydrogels . Emergent Materials. ISSN 2522-5731. 3, s. 559–566. doi: 10.1007/s42247-020-00101-5. Fulltekst i vitenarkiv Vis sammendrag

  The role of gelatin methacrylate hydrogels with varying degree of methacrylation (69% and 84%) was accessed with FTIR, NMR, microCT and subsequently exposure to human osteoblasts. The cells responded positively to the degree of methacrylation and showed attachment, growth and proliferated on both hydrogels. The cell reacted differently to the degree of methacrylation with higher proliferation on higher substitution; however, cell differentiation behavior was improved for less substitution. The secretion of late osteogenic markers (Osteoprotegerin (OPG), Osteopontin (OPN) and Osteocalcein (OCN)) and angiogenic factor Vascular endothelial growth factor (VEGF) was increased for gelatin methacrylate hydrogels with 69% degree of methacrylation and thus would be the better candidate for future bone regenerative applications amongst the three tested hydrogels

• Rumian, Lucja; Wolf-Brandstetter, Cornelia; Rößler, Sina; Reczyńska, Katarzyna; Tiainen, Hanna & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2020). Sodium alendronate loaded poly(L-lactide-co-glycolide) microparticles immobilized on ceramic scaffolds for local treatment of bone defects. Regenerative Biomaterials. ISSN 2056-3426. s. 1–10. doi: 10.1093/rb/rbaa012. Fulltekst i vitenarkiv Vis sammendrag

  Bone tissue regeneration in critical-size defects is possible after implantation of a three-dimensional scaffold and can be additionally enhanced once the scaffold is enriched with drugs or other factors supporting bone remodelling and healing. Sodium alendronate (Aln), a widely used anti-osteoporosis drug, exhibits strong inhibitory effect on bone resorption performed by osteoclasts. Thus we propose a new approach for the treatment of bone defects in craniofacial region combining biocompatible titanium dioxide (TiO2) scaffolds and poly(L-lactide-co-glycolide) (PLGA) microparticles (MPs) loaded with Aln. The MPs were effectively immobilized on the surface of the scaffolds’ pore walls by human recombinant collagen. Drug release from the scaffolds was characterised by initial burst (24 ± 6% of the drug released within first 24 h) followed by a sustained release phase (on average 5 µg of Aln released per day from day 3 to day 18). In vitro tests showed that Aln in concentrations of 5 µg/mL and 2.5 µg/mL was not cytotoxic for MG-63 osteoblast-like cells (viability between 81 ± 6% to 98 ± 3% of control) but it prevented RANKL-induced formation of osteoclast-like cells from macrophages derived from peripheral blood mononuclear cells (PBMCs), as shown by reduced fusion capability and decreased TRAP 5b activity (56 ± 5% reduction in comparison control after 8 days of culture). Results show that it is feasible to design the scaffolds providing required doses of Aln inhibiting osteoclastogenesis, reducing osteoclast activity, but not affecting osteoblast functions, which may be beneficial in the treatment of critical-size bone tissue defects.

• De-Deus, Gustavo; Simões-Carvalho, Marco; Belladonna, Felipe Goncalves; Versiani, Marcio; Silva, Emmanuel João Nogueira Leal & Cavalcante, Daniele Moreira [Vis alle 10 forfattere av denne artikkelen] (2020). Creation of well-balanced experimental groups for comparative endodontic laboratorial studies: a new proposal based on micro-CT and in silico methods . International Endodontic Journal. ISSN 0143-2885. doi: 10.1111/iej.13288. Fulltekst i vitenarkiv Vis sammendrag

  Aim To introduce a new method to select anatomically matched teeth using micro-computed tomographic (micro-CT) technology. Methodology Single-rooted mandibular incisors with a single root canal (n = 60) were selected and distributed into three experimental groups according to the method used for matching 10 pairs of teeth in each group. In group 1, the pairs of mandibular incisors were randomly selected from a pool of teeth. In group 2, teeth were paired based on the measurement of canal width 5 mm from the root apex using radiographs taken from buccolingual and mesiodistal directions. In group 3, teeth were scanned (pixel size of 14.25 μm) and pair-matched based on the anatomical aspects of the root canal, named aspect ratio (AR), volume and three-dimensional canal geometry. After allocating the specimens into groups 1 and 2, the teeth were scanned and the canal morphology evaluated as in group 3. A bivariate Pearson’s regression analysis was performed correlating the individual AR values of each pair and the correlation coefficient was used to estimate the strength of the pair-matching process. One-way ANOVA post hoc Tukey tests were applied for pair-wise comparisons at a significance level of 5%. Results The micro-CT method showed 100% of the samples having strong (80%) or very strong (20%) correlations with respect to AR values. Analysis of the radiographic method revealed strong correlation in two pairs (20%), but most of the samples had weak (30%) or neglectable (30%) correlation coefficients. The randomization method resulted in 3 pairs (30%) with very strong correlations, while 50% had weak or neglectable rates. A significant difference in correlation coefficients was observed in the micro-CT method compared to the other groups (p < 0.05), whilst no difference was detected between radiographic and randomized methods (p > 0.05). Eta squared (η 2 ) calculations demonstrated a very high effect size in the micro-CT group for selecting pairs (0.99), and lower effect sizes in the radiographic (0.67) and randomized (0.66) groups. Conclusions Micro-CT method was able to provide better control of the confounding effect that

• Strand, Onur Bülbül; Krockow, Christie Young Ae & Haugen, Håvard Jostein (2020). Hvilke kjeveortopediske metallkomponenter gir pasientreaksjoner og hvilke reaksjoner er registrert? En oversikt over metall-hypersensitivitet innen kjeveortopedien . Den norske tannlegeforenings tidende. ISSN 0029-2303. 130, s. 212–217. Vis sammendrag

  Kjeveortopedisk behandling innebærer som oftest behandling med apparaturer bestående av metaller og metallegeringer. Studier har vist at lekkasje av metallioner kan føre til allergiske reaksjoner. Målet med denne artikkelen er å gi en oversikt over forekomst av allergi, kliniske tegn og symptomer, hvilke metallioner som er allergifremkallende og behandlingsalternativer. En allergisk reaksjon skyldes en immunrespons, og de kliniske tegn og symptomer kan manifestere seg både intra- og ekstraoralt. Man bør være oppmerksom på at en allergisk pasient kan ha bare ekstraorale symptomer, selv om apparaturene befinner seg intraoralt. Dette skyldes trolig at mukosa og hud har ulike egenskaper. Nikkel forårsaker mest allergiske reaksjoner sammenlignet med andre metaller i kjeveortopedien og kan utgjøre opptil 70% av noen apparaturer. Til tross for den relativt høye prevalensen av nikkelallergi, er synlige bivirkninger på grunn av metalliske kjeveortopediske apparaturer sjeldne og oppdages hos 0,2 til 0,4 % av pasienter. De fleste allergiske pasienter kan behandles med apparaturer av rustfritt stål. Dersom pasienten fortsatt reagerer, finnes nikkelfrie alternativer. Dette kan i midlertid gi endring av behandlingstid og være lite gunstig i noen kasus

• Haugen, Håvard Jostein; Soltvedt, Brandon Michael; Nguyen, Peter Haitrieu; Rønold, Hans Jacob & Johnsen, Gaute Floer (2020). Discrepancy in alloy composition of imported and non-imported porcelain-fused-to-metal (PFM) crowns produced by Norwegian dental laboratories . Biomaterial Investigations in Dentistry. doi: 10.1080/26415275.2020.1724512. Fulltekst i vitenarkiv
• Pham, Thi Ngoc Maria Huong; Haugen, Håvard Jostein & Reseland, Janne Elin (2020). Fluoride Modification of Titanium Surfaces Enhance Complement Activation. Materials. ISSN 1996-1944. 13(3). doi: 10.3390/ma13030684. Fulltekst i vitenarkiv Vis sammendrag

  Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.

• Rahmati, Maryam; Blaker, Jonathan James; Lyngstadaas, Ståle Petter; Mano, Joao F. & Haugen, Håvard Jostein (2020). Designing multigradient biomaterials for skin regeneration. Materials Today Advances. 5. doi: 10.1016/j.mtadv.2019.100051. Fulltekst i vitenarkiv Vis sammendrag

  Skin defects are amongst the main causes of morbidity and mortality worldwide, which account for significantly high socioeconomic costs. Today, much attention is being paid to tissue engineering and biomaterials strategies for skin regeneration, and among them, there is increasing interest in using multigradient biomaterials. Gradient-based approaches are an emerging trend in tissue engineering for the homogeneous delivery of therapeutic agents by using biomaterials. Several studies have acknowledged that wound repair mechanisms could be enhanced through biomimicking physicochemical properties of different skin layers. In addition, in different layers of skin tissue, cells experience various physicochemical gradients, which potentially regulate their behaviors. Therefore, interface tissue engineering and biomaterials approaches are gaining increasing attention for skin regeneration through the incorporation of physicochemical gradients within the engineered constructs. This review first presents a necessary overview of the biological properties of skin tissue and its changes during repair in different tissue injuries. Fundamental issues and necessities of using different types of gradient scaffolds and interface tissue engineering strategies for skin regeneration are addressed. The focus of this review is on describing current progress in designing gradient scaffolds for controlling and directing cellular and molecular responses in skin tissue. The main used fabrication approaches, including both traditional and advanced methods for designing multigradient scaffolds, are also discussed.

• Tanzi, Maria Cristina; Gantz, Daniel; Bertoldi, Serena; negrini, Nicola Contessi & Haugen, Håvard Jostein (2019). Polymers and Scaffolds with Improved Blood Compatibility and Enhanced Cellular Response with Focus on Polyurethane Foams Functionalized with Amino-Amide Groups. Journal of Advanced Biotechnology and Bioengineering. ISSN 2311-1755. 7, s. 18–29. doi: 10.12970/2311-1755.2019.07.03. Vis sammendrag

  Biofunctionalization of biomaterial substrates has gained increasing interest in Tissue Engineering (TE) to create functional scaffolds with improved performances and capable of directing cellular behaviour. This work takes into account the functionalization of polyurethane (PU) foams with an active molecule, the diamino-diamide diol PIME, purposely designed to be inserted in the structure of linear PUs and previously found to be able to improve hemo-, cytocompatibility and bacterial resistance of these biomaterials. Here, for the first time, the gas foaming process previously set up for biocompatible PU foams was modified, and different methods were tested to obtain structurally stable foams containing PIME. The obtained foams and respective controls showed appropriate porosity (Ø = 407 ÷ 589 ± 30 m; > 90% open porosity), similar density ( 0.14 g/cm3) and hydrophilicity (water uptake  300%) and adequate compressive properties (E = 0.058 ÷ 0.170 Mpa; Hysteresis Area = 2.07 ÷ 5.59 x 10-3 J/cm3, < 5% residual deformation). PU foams containing PIME exhibited a more spherical pore geometry; the foam obtained by applying a higher stirring speed during synthesis (PU-3kP) exhibited a lower mean pore size and an elastic modulus higher than that of the other foams. FT-IR spectroscopy indicated the presence of new absorption bands and structural changes attributable to the presence of PIME. Cytotoxicity tests performed with the cell line L929 verified the absence of cytotoxic effects. All foams were able to support L929 fibroblasts proliferation and vitality for seven days; PIME-containing foams showed higher values of cell proliferation (p<0.05) that the respective controls. Keywords: Polyurethane foams, scaffold, functionalization, bioactive molecule, PIME, open porosity, compressive properties, cell interactions, L929, fibroblasts.

• Sanz, Mariano; Dahlin, Christer; Apatzidou, Danae; Artzi, Zvi; Bozic, Darco & Calciolari, Elena [Vis alle 19 forfattere av denne artikkelen] (2019). Biomaterials and regenerative technologies used in bone regeneration in the craniomaxillofacial region: Consensus report of group 2 of the 15th European Workshop on Periodontology on Bone Regeneration. Journal of Clinical Periodontology. ISSN 0303-6979. 46(21), s. 82–91. doi: 10.1111/jcpe.13123. Fulltekst i vitenarkiv
• Zhu, Hao; Xiongfa, Ji; Hanfeng, Guan; Liming, Zhao; Libo, Zhao & Changyu, Liu [Vis alle 12 forfattere av denne artikkelen] (2019). Tantalum Nanoparticles Reinforced Polyetheretherketone Shows Enhanced Bone Formation. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 101, s. 232–242. doi: 10.1016/j.msec.2019.03.091. Fulltekst i vitenarkiv
• Schweikle, Manuel; Bjørnøy, Sindre Hove; Van Helvoort, Antonius; Haugen, Håvard Jostein; Sikorski, Pawel & Tiainen, Hanna (2019). Stabilisation of amorphous calcium phosphate in polyethylene glycol hydrogels. Acta Biomaterialia. ISSN 1742-7061. 90, s. 132–145. doi: 10.1016/j.actbio.2019.03.044. Fulltekst i vitenarkiv
• Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter; Rossi, Filippo & Perale, Giuseppe (2019). Bone grafts: which is the ideal biomaterial? Journal of Clinical Periodontology. ISSN 0303-6979. 46(21), s. 92–102. doi: 10.1111/jcpe.13058. Fulltekst i vitenarkiv
• Pham, Thi Ngoc Maria Huong; Haugen, Håvard Jostein; Rinna, Alessandra; Ellingsen, Jan Eirik & Reseland, Janne Elin (2019). Hydrofluoric acid treatment of titanium surfaces enhance the proliferation of human gingival fibroblasts. Journal of Tissue Engineering. ISSN 2041-7314. 10. doi: 10.1177/2041731419828950. Fulltekst i vitenarkiv Vis sammendrag

  The attachment of implants rely on bone and soft tissue biocompatibility. The aim was to investigate the effect of fluoride modified metallic titanium (Ti) surfaces (Ti-F) on proliferation and differentiation of human gingival fibroblasts (HGF). HGF cells were exposed to hydrofluoric acid (HF) modified Ti coins (Ti-F) for 1, 3, 7, 14 and 21d, and untreated coins were used as controls. A 5-6 fold increase in the proliferation of HGF on Ti-F compared to Ti surfaces was observed. Enhanced gene expression of IL-6 and OPG was found at 7 d. Increased levels of sclerostin, IL- 6 and OPG in the media from HGF cultured on Ti-F coins were found compared to controls. Our results confirm that HF modified surface may indirectly enhance the firm attachment of implant surface to junction epithelium, soft tissue epithelium, which would give protection for underlying osseous structures making osseointegration of the dental implant possible.

• Klemm, Anne; Gomez-Florit, Manuel; Almeida Carvalho, Patricia; Wachendörfer, Mattis; Gomes, Manuela E. & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2019). Grain boundary corrosion in TiO2 bone scaffolds doped with group II cations. Journal of the European Ceramic Society. ISSN 0955-2219. 39, s. 1577–1585. doi: 10.1016/j.jeurceramsoc.2018.12.055. Fulltekst i vitenarkiv
• Zhang, Xianbin; Tiainen, Hanna & Haugen, Håvard Jostein (2019). Comparison of titanium dioxide scaffold with commercial bone graft materials through micro-finite element modelling in flow perfusion. Medical and Biological Engineering and Computing. ISSN 0140-0118. 57(1), s. 311–324. doi: 10.1007/s11517-018-1884-2. Fulltekst i vitenarkiv Vis sammendrag

  TiO2 scaffolds have previously shown to have promising osteoconductive properties in previous in vivo experiments. Appropriate mechanical stimuli can further promote this osteoconductive behaviour. However, the complex mechanical environment and the mechanical stimuli enhancing bone regeneration for porous bioceramics have not yet been fully elucidated. This paper aims to compare and evaluate mechanical environment of TiO2 scaffold with three commercial CaP biomaterials, i.e. Bio-Oss, Cerabone and Maxresorb under simulated perfusion culture conditions. The solid phase and fluid phase were modelled as linear elastic material and Newtonian fluid, respectively. The mechanical stimulus was analysed within these porous scaffolds quantitatively. The results showed that the TiO2 had nearly heterogeneous stress distributions, however lower effective Young’s modulus than Cerabone and Maxresorb. The permeability and wall shear stress (WSS) for the TiO2 scaffold was significantly higher than other commercial bone substitute materials. Maxresorb and Bio-Oss showed lowest permeability and local areas of very high WSS. The detailed description of the mechanical performance of these scaffolds could help researchers to predict cell behaviour and to select the most appropriate scaffold for different in vitro and in vivo performances.

• Virto, Leire; Cano, Pilar; Jiménez-ortega, Vanesa; Fernández-mateos, Pilar; González, Jerián & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2018). Melatonin as adjunctive therapy in the treatment of periodontitis associated with obesity. Journal of Clinical Periodontology. ISSN 0303-6979. 45(11), s. 1336–1346. doi: 10.1111/jcpe.13013. Fulltekst i vitenarkiv Vis sammendrag

  Aims To study the effect of adjunctive systemic administration of melatonin to standard mechanical periodontal therapy in obese rats with experimental periodontitis. Materials and methods In 42 Wistar rats with an initial body weight of 180 g., half (n=21) were fed with a high‐fat diet to induce obesity. In both obese and normal‐weight groups, experimental periodontitis was subsequently induced through oral gavages with a combination of Porphyromona gingivalis and Fusobacterium nucleatum. Both groups were randomly allocated to either, no treatment or periodontal treatment consisting on standard mechanical debridement, with either adjunctive chlorhexidine or melatonin. Outcomes were evaluated by the changes in clinical parameters (probing depth modified gingival index, plaque dental index and bleeding on probing), in bone resorption and in the levels of biomarkers in plasma and in gingival tissue (inflammatory cytokines, insulin, leptin, osteocalcin, osteopontin, plasminogen activator inhibitor‐1, intercellular adhesion molecule 1, E‐selectin and lipids). Results In the obese‐periodontitis group, adjunctive melatonin administration resulted in reduced gingival inflammation and bleeding on probing, with significant reductions in probing depth and enhanced bone repair demonstrated by Micro‐CT (15% reduction in alveolar bone destruction) when compared with the same group treated with adjunctive CHX or the normal‐weight rats with either melatonin or CHX. In this melatonin‐treated obese‐periodontitis group, a significant impact on biochemical biomarkers was also demonstrated in both gingival and plasma samples, when compared with the other groups, with significant reductions in pro‐inflammatory cytokines. Conclusions Adjunctive melatonin therapy significantly reduced alveolar bone loss and exerted a protective anti‐inflammatory effect mainly in those experimental animals affected by the co‐morbidity of periodontitis and obesity.

• Kamyar, Negin; Greenhalgh, Ryan; Nascimentoa, Tatiana R. L; Medeiros, Eliton S; Matthews, Peter D & Nogueira, Liebert Parreiras [Vis alle 9 forfattere av denne artikkelen] (2018). Exploiting Inherent Instability of 2D Black Phosphorus for Controlled Phosphate Release from Blow-Spun PLGA Nanofibers. ACS Applied Nano Materials. ISSN 2574-0970. 1, s. 4190–4197. doi: 10.1021/acsanm.8b00938. Vis sammendrag

  Efforts have been made to stabilize black phosphorus (BP) to utilize its tunable band gap and anisotropic mechanical properties. Here, the intrinsic instability of BP is exploited for controlled therapeutic ion release, namely phosphate. BP was incorporated into degradable poly (lactide-co-glycolide) fibers via solution blow spinning. Raman spectroscopy confirmed the incorporation of 2D-BP into the nanocomposite along with ICP-AES. It was also demonstrated that modifying the initial loading of 2D-BP in the PLGA fibers permitted tunable release rates of phosphate ions over an 8 weeks in vitro study.

• Virto , Leire; Haugen, Håvard Jostein; Fernández-mateos, Pilar; Cano, Pilar; González, Jerián & Jiménez-ortega, Vanesa [Vis alle 8 forfattere av denne artikkelen] (2018). Melatonin expression in periodontitis and obesity: An experimental in-vivo investigation. Journal of Periodontal Research. ISSN 0022-3484. 53(5), s. 825–831. doi: 10.1111/jre.12571. Fulltekst i vitenarkiv Vis sammendrag

  Background and Objective: Melatonin deficiency has been associated with obesity and systemic inflammation. This study aims to evaluate whether melatonin could interfere with the mechanisms of co-morbidity linking obesity and periodontitis. Material and Methods: Twenty-eight male Wistar rats were randomly divided in 4 groups: control group (Con) (fed with standard diet); high-fat diet group (HFD) (fed with a diet containing 35.2% fat); Con group with induced periodontitis (Con-Perio) and HFD group with induced periodontitis (HFD-Perio). To induce periodontitis, the method of oral gavages with Porphyromonas gingivalis ATCC W83K1 and Fusobacterium nucleatum DMSZ 20482 was used. Circulating melatonin levels were analyzed by multiplex immunoassays. Periodontitis was assessed by alveolar bone loss (microcomputed tomography and histology) and by surrogate inflammatory outcomes (periodontal pocket depth, modified gingival index and plaque dental index). Results: Plasma melatonin levels were significantly decreased (P < .05) in the obese rats with periodontitis when compared with controls or with either obese or periodontitis rats. Alveolar bone loss increased 27.71% (2.28 µm) in HFD-Perio group compared with the Con group. The histological analysis showed marked periodontal tissue destruction with osteoclast activity, particularly in the HFD-Perio group. A significant negative correlation (P < .05) was found between periodontal pocket depth, modified gingival index and circulating melatonin levels. Conclusion: Obese and periodontitis demonstrated significantly lower melatonin concentrations when compared with controls, but in obese rats with periodontitis these concentrations were even significantly lower when compared with either periodontitis or obese rats. These results may indicate that melatonin deficiency could be a key mechanism explaining the co-morbidity effect in the association between obesity and periodontitis.

• Johnsen, Gaute Floer; Sunde, Pia Titterud & Haugen, Håvard Jostein (2018). Validation of contralateral premolars as the substrate for endodontic comparison studies. International Endodontic Journal. ISSN 0143-2885. 51(8), s. 942–951. doi: 10.1111/iej.12902. Fulltekst i vitenarkiv Vis sammendrag

  AIM: To use micro-CT technology and metrology software to validate the use of contralateral premolars as samples in endodontic comparison studies by comparing them before and after canal instrumentation with one instrumentation system. Furthermore, to determine if contralateral premolar roots will yield non-significantly different outcomes regarding shaping ability (volume), degree of twisting, and three-dimensional shape changes. The null-hypothesis (H0 ) is that there are no differences between the contralateral premolar roots pre- or post-instrumentation. METHODOLOGY: Twenty-eight extracted human contralateral premolars (n=44 contralateral roots) from 12 donor patients were scanned with microcomputed tomography before and after instrumentation. Root canal lengths (RCL) were measured visually using a dental operating microscope, electronic apex locator, and micro-CT scans. Data were analyzed statistically for differences between pre- and post-instrumentation. RESULTS: Instrumentation increased the volume of the canals significantly (P<0.05). Degree of twisting for a majority (83%) of the contralateral roots pairs did not change significantly (P>0.05). There was no significant difference (P>0.05) in the shape deviation analysis between contralateral pairs. There was no significant difference (P>0.05) for root canal length between the contralateral pairs for any of the three endometric methods. CONCLUSION: Contralateral premolar root canals were associated with similar changes in terms of volume, three-dimensional shape, and degree of twisting from pre- to post-instrumentation. There was no difference between the contralateral premolar root pairs pre- and post-instrumentation, and the study validates contralateral premolars as samples for root canal comparison studies. The null-hypothesis (H0 ) could not be rejected. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

• Sike, Adam; Wengenroth, Jonas; Upite, Jolanta; Brüning, Thomas; Eiriz Delgado, Iván & Santha, Petra [Vis alle 11 forfattere av denne artikkelen] (2017). Improved method for cannula fixation for long-term intracerebral brain infusion. Journal of Neuroscience Methods. ISSN 0165-0270. 290, s. 145–150. doi: 10.1016/j.jneumeth.2017.07.026.
• Hussain, Badra; Thieu, Minh Khai Le; Johnsen, Gaute Floer; Reseland, Janne Elin & Haugen, Håvard Jostein (2017). Can CAD/CAM resin blocks be considered as substitute for conventional resins? Dental Materials. ISSN 0109-5641. 33(12), s. 1362–1370. doi: 10.1016/j.dental.2017.09.003. Vis sammendrag

  Objective Dentists are facing a myriad of new CAD/CAM product for dental filling therapies. Are the new materials any worthwhile using? Are they succeeding the standard filling materials? Here we compare for the first time the new resin-composite blocks (RCBs) with conventional materials (Filtek Z250 and Tetric EvoCeram). Methods The material were tested for residual monomer elution by HPLC, thermogravimetric analysis (TG) was used to determine the percentage of fillers by weight, hardness was evaluated by Vickers method, morphology of fillers and distribution in the matrix were examined by scanning electron microscopy (SEM), elemental analysis for elemental determination of the filler particles was performed by energy-dispersive X-ray spectroscopy (EDX) cytotoxicity using human gingival fibroblasts and an epithelial cell line. Results The RBC outperformed conventional composite regarding mechanical characteristics (hardness) and monomer eluation, but showed some worrisome results regarding cytotoxicity. Significance The cost benefit is not in favour of RBCs in comparison to conventional composites, as the cytotoxicity was found higher for RBCs.

• Cama, Giuseppe; Nkhwa, Shatani; Gharibi, Borzo; Lagazzo, Alberto; Cabella, Roberto & Carbone, Christina [Vis alle 10 forfattere av denne artikkelen] (2017). The role of new zinc incorporated monetite cements on osteogenic differentiation of human mesenchymal stem cells. Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 78, s. 485–494. doi: 10.1016/j.msec.2017.04.086. Vis sammendrag

  β-Tricalcium phosphate particles were sintered in the presence of different amounts (0–0.72 mol) of zinc oxide (ZnO) to prepare zinc doped β-TCP (Znβ-TCP) particles for further use in novel monetite (DCPA: CaHPO4) zinc incorporated bone cements with osteogenic differentiation potential towards human mesenchymal stem cells (hMSCs). XRD analysis of zinc incorporated cements prepared with β-TCP reagent particles doped with different amount of ZnO (i.e. 0.03, 0.09 and 0.18 mol ZnO) revealed the presence of unreacted Znβ-TCP and monetite. Furthermore, it was shown that zinc ions preferentially occupied the β-TCP crystal lattice rather than the monetite one. Release experiments indicated a burst release of ions from the different fabricated cements during the first 24 h of immersion with zinc concentrations ranging between 85 and 100% of the total concentration released over a period of 21 days. Cell proliferation significantly increased (P < 0.05) on zinc incorporated monetite respect to control samples (Zinc-free cement) at 7 and 14 days post seeding. The expression of Runx-2 was significantly up regulated (P < 0.05) in the case of cells seeded on monetite prepared with β-TCP doped with 0.03 moles of ZnO. On the other hand, the cell mineralization as well as the expression of osteogenic marker genes ALP and OSC decreased significantly (P < 0.05) at 14 days post cell seeding. In conclusion, these results suggest that the zinc ions released from the cements during the first 24 h of culture played a critical role in regulating the osteogenic differentiation of hMSCs

• Johnsen, Gaute Floer; Dara, Sazan; Asjad, Sameenah; Sunde, Pia Titterud & Haugen, Håvard Jostein (2017). Anatomic Comparison of Contralateral Premolars. Journal of Endodontics. ISSN 0099-2399. 43(6), s. 956–963. doi: 10.1016/j.joen.2017.01.012. Vis sammendrag

  Introduction The comparative anatomy of contralateral premolars has not been previously studied. The purpose of this micro–computed tomography investigation was to qualitatively and quantitatively assess and compare the morphology of contralateral premolars in terms of length, canal width, dentinal thicknesses, accessory canals, root canal configurations, isthmi, C-shapes, root canal orifices, and apical foramina. The null hypothesis (H0) is that contralateral premolars are more morphologically similar than randomly assigned pairs for simple morphometric measurements (lengths, canal widths, and dentinal thickness). Methods Forty-one intact premolar pairs (n = 82) extracted from 28 patients were scanned with micro–computed tomography and reconstructed. Quantitative comparative assessment of measurements was performed by pairwise statistical analysis of contralateral and random pairs with Student t test and one-sample t test. All measured parameters (lengths, widths, and thicknesses) were normalized by Z score so that they could all be compared on a common scale. A correlation study was also performed. Canal configurations and isthmi were classified according to preexisting classification schemes. The number and location of accessory canals and apical foramina were registered and compared. Results/Conclusions Contralateral premolar pairs demonstrated a high degree of similarity in terms of the linear measurements (lengths, widths, and thicknesses). The apical portion (foramina, C-shapes, and accessory canals) did not demonstrate bilateral symmetry. The comprehensive statistical analysis of the normalized parameters by Z score showed no statistically significant differences between the contralateral premolar pairs. The null hypothesis (H0) was accepted.

• Rumian, Lucja; Tiainen, Hanna; Cibor, Urszula; Krok-Borkowicz, Małgorzata; Brzychczy-Włoch, Monika & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2017). Ceramic scaffolds with immobilized vancomycin-loaded poly(lactide-co-glycolide) microparticles for bone defects treatment. Materials Letters. ISSN 0167-577X. 190, s. 67–70. doi: 10.1016/j.matlet.2016.12.113.
• Johnsen, Gaute Floer; Thieu, Minh Khai Le; Hussain, Badra; Pamula, Elzbieta; Reseland, Janne Elin & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2017). Own brand label restorative materials - A false bargain? Journal of Dentistry. ISSN 0300-5712. 56, s. 84–98. doi: 10.1016/j.jdent.2016.11.004. Vis sammendrag

  Objectives This study aims at evaluating and comparing mechanical, chemical, and cytotoxicological parameters of a commercial brand name composite material against two ‘own brand label' (OBL) composites. Methods Parameters included depth of cure, flexural strength, degree of conversion, polymerization shrinkage, filler particle morphology and elemental analyses, Vickers hardness, surface roughness parameters after abrasion, monomer elution, and cytotoxicity. Results The conventional composite outperformed the OBLS in terms of depth of cure (p < 0.001), degree of cure at the first and last time intervals (p < 0.001), hardness (p < 0.001), and post-abrasion roughness (p < 0.05). The polymerization volumetric shrinkage ranged from 2.86% to 4.13%, with the highest shrinkage seen among the OBLs. Both Monomer elution from the OBLs was statistically significantly higher (p<0.001). Statistically significantly higher cytotoxicity combined with altered morphology and loss of confluence was detected in the cells exposed to extracts from the OBLs. Conclusions The OBLs were in general outdone by the conventional composite. Clinical Significance: OBLs restorative materials have become pervasive in the dental market. Manufacturers often promise equal or better characteristics than existing brand-name composites, but at a lower price. Dentists are highly recommended to reconsider utilization of OBLs lacking sound scientific scrutiny, and our findings underscore this recommendation

• Barrantes Bautista, Alejandro; Wengenroth, Jonas; Arnebrant, Thomas & Haugen, Håvard Jostein (2017). Poly-L-lysine/heparin multilayer coatings prevent blood protein adsorption. Journal of Colloid and Interface Science. ISSN 0021-9797. 485, s. 288–295. doi: 10.1016/j.jcis.2016.09.046. Fulltekst i vitenarkiv
• Wiig, Elisabeth; Reseland, Janne Elin; Østbye, Kjartan; Haugen, Håvard Jostein & Vøllestad, Leif Asbjørn (2016). Variation in Lateral Plate Quality in Threespine Stickleback from Fresh, Brackish and Marine Water: A Micro-Computed Tomography Study. PLOS ONE. ISSN 1932-6203. 11(10). doi: 10.1371/journal.pone.0164578.
• Johnsen, Gaute Floer; Sundnes, Joakim; Wengenroth, Jonas & Haugen, Håvard Jostein (2016). Methodology for Morphometric Analysis of Modern Human contralateral Premolars. Journal of computer assisted tomography. ISSN 0363-8715. 40(4), s. 617–625. doi: 10.1097/RCT.0000000000000417.

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• Haugen, Håvard Jostein & Bertoldi, Serena (2017). 3D porous structures: porosity and other relevant morphological parameters. Woodhead Publishing Limited. ISBN 9780081007433. 500 s. Vis sammendrag

  Three-dimensional (3D) porous structures find applications in the biomedical field mainly as scaffolds for tissue engineering and regenerative medicine, as for most tissues (eg, bonetissue, bloodvessel, muscletissue) aporoussupportguidinggrowingtissueiscrucial. Their characterization appears to be fundamental, as the architectural parameters, porosity in particular, but also pore size and interconnectivity [1, 2], strongly affect the mechanical and biological performance of the 3D structures [2, 3], and thus their functionality. Effective techniques, able to provide information on scaffold morphological properties, are required at the initial stages of research and development, so to design scaffolds with the optimal properties for the specific application. In addition, since the biological performance of the scaffolds should be evaluated with respect to their ability during time to favor cell adhesion, proliferation, and extracellular matrix production, an analytical technique capable of monitoring the evolving steps of a cell/tissuebiomaterial interaction experiment, both in vitro and in vivo, can be very useful. The need for measurements and standard rules to be applied for scaffold characterization is well identified by scientists and regulators [4], and the debate about that is vivid. Among the morphological parameters to be analyzed (Table 2.1), porosity is the most often quoted [5], as it would influence scaffold mechanical properties and scaffold-cell interactions.

• Gordeladze, Jan Oxholm; Landin, Maria Augusta Dos S Silva; Johnsen, Gaute Floer; Haugen, Håvard Jostein & Osmundsen, Harald (2017). Vitamin K2 and its Impact on Tooth Epigenetics. IntechOpen. ISBN 978-953-51-3020-8. 27 s. Vis sammendrag

  The impact of nutritional signals plays an important role in systemic-based «models» of dental caries. Present hypotheses now focus both on the oral environment and other organs, like the nervous system and brain. The tooth is subjected to shear forces, nourishing and cleansing, and its present “support system” (the hypothalamus/parotid axis) relays endocrine signaling to the parotid gland. Sugar consumption enhances hypothalamic oxidative stress (ROS), reversing dentinal fluid flow, thus creating an enhanced vulnerability to the oral bacterial flora. The acid, produced by the oral bacterial flora, then leads to erosion of the dentine, and an irreversible loss of dental enamel layers. This attack brings about inflammatory responses, yielding metalloproteinase-based “dissolution”. However, vitamin K2 (i.e. MK-4/MK-7) may come to the rescue with its antioxidant property, locally (mouth cavity) or systemically (via the brain), thus sustaining/preserving hormone-induced dentinal fluid flow (encompassing oxidative stress) and boosting/magnifying bodily inflammatory responses. However, sugars may also reduce the tooth’s natural defences through endocrine signaling, thus enhancing acid-supported enamel dentine erosion. Vitamin K2 sustains and improves the salivary buffering capacity via its impact on the secretion/flow of calcium and inorganic phosphates. Interestingly, primitive cultures’ diets (low-sugar and high-K2 diets) preserve dental health.

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• Zhu, Hao; Ji, Xiongfa; Guan, Hanfeng; Zhao, Liming; Zhao, Libo & Liu, Changyu [Vis alle 12 forfattere av denne artikkelen] (2021). Corrigendum to “Tantalum nanoparticles reinforced polyetheretherketone shows enhanced bone formation” [Mater. Sci. Eng. C 101 (2019) 232–242] (Materials Science & Engineering C (2019) 101 (232–242), (S0928493118321453), (10.1016/j.msec.2019.03.091)). Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 128. doi: 10.1016/j.msec.2021.112332.
• Abadpour, Shadab; Niemi, Essi Maria; Strid Orrhult, Linnea; Parreiras Nogueira, Liebert; Haugen, Håvard Jostein & Josefsen, Dag [Vis alle 10 forfattere av denne artikkelen] (2021). 307.7: 3D Bioprinting of Functional Islets With Adipose-derived Stromal Cells in an Alginate/Nanocellulose Scaffold. Transplantation. ISSN 0041-1337. s. S25–S25. doi: 10.1097/01.tp.0000804424.50335.7e.
• Haugen, Håvard Jostein & Engqvist, Håkan (2021). first editorial for the new journal Scandinavian Journal of Materials Science. Scandinavian Journal of Materials Science. ISSN 2004-2124. 1(1). doi: 10.2340/scandjms.v1.883.
• Rahmati, Maryam; Johannes Frank, Matthias; Martin Walter, Sebastian; Cabrer Monjo, Marta; Satué, Maria & Reseland, Janne Elin [Vis alle 8 forfattere av denne artikkelen] (2021). Osteo-immune responses to the enamel matrix derivative coating layer on titanium-based implants .
• Rahmati, Maryam; Palombo, David; Vignoletti, Fabio; Javier, Sanz-Esporrin; Haugen, Håvard Jostein & Sanz, Mariano (2021). Tissue healing around PRAMA vs. PREMIUM implants .
• Johannes Frank, Matthias; Walter, Martin Sebastian; Cabrer Monjo, Marta; Satué, Maria; Reseland, Janne E & Ståle Petter, Lyngstadaas [Vis alle 7 forfattere av denne artikkelen] (2021). Osteo-immune responses to the enamel matrix derivative coating layer on titanium-based implants .
• Haugen, Håvard Jostein & Parreiras Nogueira, Liebert (2021). Novel contrast agent for visualisation of soft tissue with X-ray imaging.
• Haugen, Håvard Jostein; Parreiras Nogueira, Liebert & Sunde, Pia Titterud (2021). Use of novel contrast enhanced agent for tissue engineering.
• Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter; Monjo Cabrer, Marta & Perale, Giuseppe (2021). XENO-HYBRID BONE GRAFT RELEASING MATRIX-DERIVED INTRINSICALLY DISORDERED PROTEINS PROMOTES OSTEOGENIC DIFFERENTIATION OF HMSCS.
• Haugen, Håvard Jostein; Thieu, Minh Khai Le; Sanz-Esporrin, Javier; Sanz Alonso, Mariano & Verket, Anders (2021). GUIDED BONE REGENERATION OF CHRONIC NON-CONTAINED BONE DEFECTS USING A VOLUME STABLE POROUS TIO2 SCAFFOLD: AN EXPERIMENTAL IN VIVO STUDY .
• Linke, Dirk; Økstad, Ole Andreas; Haugen, Håvard Jostein & Winther-Larsen, Hanne Cecilie (2021). The exchange program MISC - Options for internships at the University of Oslo (with descriptions of current projects).
• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Wieland, D.C. Florian; Plumhoff, Berit Zeller & Iskhahova, Kamila [Vis alle 7 forfattere av denne artikkelen] (2021). 2&3D imaging of osteogenesis and macrophage polarization in magnesium-based alloys .
• Rahmati, Maryam; Stoetzel, Sabine; El Khassawna, Thaqif; Wieland, Florian; Iskhahova, Kamila & Plumhoff, Berit Zeller [Vis alle 7 forfattere av denne artikkelen] (2021). EARLY INFLAMMATORY RESPONSES TO MAGNESIUM-BASED ALLOYS.
• Haugen, Håvard Jostein; Hussain, Badra & Bjørkeng, Astrid Skiftesvik (2021). Er biomaterialer kroppens indre plaster? [Internett]. Universitetsplassen Podcast. Vis sammendrag

  Ting går i stykker. Også i kroppen. Men medisinsk forskning har gjort det mulig å erstatte noen ødelagte deler med biomaterialer. I denne podkasten forklarer forskerne Håvard Haugen og Badra Hussein hva biomaterialer er og hvordan de brukes - og ikke minst hvordan de forsker på nye biomaterialer for å lage nytt bein.

• Farè, Silvia; Torricelli, Paola; Giavaresi, Gianluca; Bertoldi, Serena; Alessandrino, Antonio & Villa, Tomaso [Vis alle 10 forfattere av denne artikkelen] (2020). Corrigendum to “In vitro study on silk fibroin textile structure for Anterior Cruciate Ligament regeneration” [Mater. Sci. Eng. C (2013) 3601–360] (Materials Science and Engineering C, (S0928493113002415), (10.1016/j.msec.2013.04.027)). Materials Science and Engineering C: Materials for Biological Applications. ISSN 0928-4931. 116. doi: 10.1016/j.msec.2020.111193.
• Pawlik, A; Sulka, G.D. & Haugen, Håvard Jostein (2019). Fabrication and characterization of doxycycline-coated nanoporous TiO2 layers.
• Haugen, Håvard Jostein (2019). Novel nano-computed tomography techniques applied to dental research.
• Esporrin, Javier Sanz; Haugen, Håvard Jostein & Sanz, Mariano (2019). Experimental Peri-implantitis around Titanium Implants with a Modified Titanium Surface with a Phosphonic Acid Monolayer. NanoCT Results of an Experimental In-vivo Investigation.
• Haugen, Håvard Jostein (2019). Micro-porosities Revealed in Hybrid Dental Composites by Nano X-ray Tomography.
• Haugen, Håvard Jostein (2019). Micro and nano-CT applied to dental research.
• Haugen, Håvard Jostein (2019). Structural and Bio-inspired Bioceramic Materials and Implants.
• Tanzi, Maria Cristina; gantz, daniel; Bertoldi, Serena; negrini, Nicola Contessi & Haugen, Håvard Jostein (2019). FUNCTIONALIZED POLYURETHANE FOAMS AS TISSUE SCAFFOLDS WITH ENHANCED CELLULAR RESPONSE. Vis sammendrag

  Recentadvancementsintissueengineeringrequirethedevelopmentoftailoredscaffoldswithphysicochemicalcuesabletopromotespecificandpositiveresponsesoncellsofthetargettissue • Biomedicalpolyurethanes(PU)exhibitgoodresilienceandfatiguestrength,goodcytoandhemocompatibilityandlowbacteriaadhesion;PUfoamscanbedesignedassuitablescaffoldsintissueengineering[1-3] • The diamino-diamide diolPIME (figure)canform stable complexes with heparin, due to the protonation of its tertiary amino groups at a physiological pH, has excellent cytocompatibility and can promote cells adhesion and proliferation on linear PUs containing

• Haugen, Håvard Jostein & Reseland, Janne Elin (2018). In vivo performance of a highly porous titanium dioxide bone scaffold and its way to the medical device market.
• Klemm, Anne; Haugen, Håvard Jostein & Tiainen, Hanna (2018). Grain boundary corrosion in Sr and Ca doped TiO2 bone scaffold. Vis sammendrag

  Porous TiO2 scaffolds as a bone support structure showed high porosity combined with high compressive strength1. Doping ceramic scaffolds with Ca or Sr leads to even higher compressive strength and formation of impurity rich grain boundaries2. Grain boundary dissolution can occur during bone healing, because of a local pH drop3. To improve the corrosion resistance of cationic doped TiO2 scaffolds, the objective of this study was to investigate the corrosion depending on doping concentration to evaluate corrosion mechanisms.

• Schweikle, Manuel; Bjørnøy, Sindre Hove; Lyngstadaas, Ståle Petter; Haugen, Håvard Jostein; Van Helvoort, Antonius & Sikorski, Pawel [Vis alle 7 forfattere av denne artikkelen] (2018). Self-mineralising synthetic injectable hydrogels for bone repair.
• de Lange, Thomas; Haugen, Håvard Jostein & Møystad, Anne (2018). Digital Prospects of Formative and Summative Assessment in Dental Education: Technological Opportunities and Challenges. Vis sammendrag

  Abstract: It is a well-known fact that assessment is an important driver for student learning. In the advancement of digital technologies, the question is how digital resources can be used to improve formative and summative assessment in ways that support student learning as well as ensuring knowledge standards for future dental professionals. Two experimental studies on digital assessment have been conducted at the Faculty of Dentistry, University of Oslo. Firstly, digitized formative assessment based on Multiple Choice questionnaires (MCQ) was implemented in testing students’ knowledge from prior parts of their education. Secondly, a pilot-study of a digitized summative assessment in oral radiology was conducted to identify benefits and challenges in applying entirely digital based exams. Results from these experiments are presented and discussed in relation to research on assessment and student learning.

• Haugen, Håvard Jostein; Reseland, Janne Elin & Gomez-Florit, Manuel (2018). The effect of IDP coated biomaterial.
• Haugen, Håvard Jostein & Ellingsen, Jan Eirik (2018). Her skapes fremtidens arbeidsplasser i bioteknologi. [Fagblad]. Apollon. Vis sammendrag

  I det nye laboratoriet Sharelab på Blindern møtes forskere, bedriftsutviklere og investorer. De håper å skape hundrevis av kommersielle arbeidsplasser innen bioteknologi.

• Schneider, Kenneth; Haugen, Håvard Jostein & Linke, Dirk (2017). Assay Development: Adhesin Interaction with Implant Materials.
• Gomez-Florit, Manuel; Reseland, Janne Elin & Haugen, Håvard Jostein (2017). Synthetic intrinsically-disordered-peptides for bone tissue engineering. Vis sammendrag

  DISCUSSION & CONCLUSIONS: Results show that synthetic IDPs are able to influence hOBs biomineralization. The earlier upregulation of osteogenic markers together with the decreased wound area induced by P2 indicate that it has potential to induce bone regeneration. Bone related proteins, cytokines and chemokines released to culture media are already being analysed to confirm these results. IDPs might be a reliable option to develop new biomaterials and tissue engineering strategies

• Haugen, Håvard Jostein; Johnsen, Gaute Floer; Reseland, Janne Elin; Hussain, Badra & Thieu, Minh Khai Le (2017). Can CAD/CAM Resin Blocks be Considered as a Substitute to Conventional Resins?
• Schneider, Kenneth; Saragliadis, Athanasios; Haugen, Håvard Jostein & Linke, Dirk (2016). Aggregatibacter Adhesins and their interaction with implant materials.

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