Elisabeth Aurstad Riksen

• Schröder, Maria; Riksen, Elisabeth Aurstad; He, Jianying; Skallerud, Bjørn Helge; Møller, Mona Elisabeth & Lian, Aina-Mari [Vis alle 8 forfattere av denne artikkelen] (2020). Vitamin K2 modulates vitamin D induced mechanical properties of human 3D bone spheroids in vitro. JBMR Plus. ISSN 2473-4039. 4(9). doi: 10.1002/jbm4.10394. Fulltekst i vitenarkiv
• Riksen, Elisabeth Aurstad; Landin, Maria Augusta Dos S Silva; Reppe, Sjur; Nakamura, Yoikio; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2014). Enamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralization. International Journal of Molecular Sciences. ISSN 1661-6596. 15(5), s. 7731–7749. doi: 10.3390/ijms15057731. Fulltekst i vitenarkiv
• Riksen, Elisabeth Aurstad; Petzold, Christiane; Brookes, Steven; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2011). Human osteoblastic cells discriminate between 20-kDa amelogenin isoforms. European Journal of Oral Sciences. ISSN 0909-8836. 119, s. 357–365. doi: 10.1111/j.1600-0722.2011.00912.x.
• Riksen, Elisabeth Aurstad; Kalvik, Anne; Brookes, Steven; Hynne, Astrid; Snead, Malcolm L. & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2011). Fluoride reduces the expression of enamel proteins and cytokines in an ameloblast-derived cell line. Archives of Oral Biology. ISSN 0003-9969. 56(4), s. 324–330. doi: 10.1016/j.archoralbio.2010.10.024. Vis sammendrag

  Abstract OBJECTIVE: To investigate the effects of two different fluoride concentrations on the expression of enamel proteins, alkaline phosphatase (ALP), cytokines and interleukins by an ameloblast-derived cell line. METHODS: Murine ameloblast-derived cells (LS-8), mouse odontogenic epithelia, were exposed to 1 or 5ppm sodium fluoride (NaF) (0.46 and 2.25ppm F, respectively) for 1, 3 and 7 days. The effect of NaF on the mRNA expression of enamel proteins was quantified; the secretion of cytokines, and interleukins, and the alkaline phosphatase (ALP) activity, into the cell culture medium was measured and compared to untreated controls. The effect on cell growth after 1- and 3-days in culture was measured using BrdU incorporation. RESULTS: Fluoride at 2.25ppm reduced mRNA expression of the structural enamel matrix proteins amelogenin (amel), ameloblastin (ambn), enamelin (enam), and the enamel protease matrix metallopeptidase-20 (MMP-20). Similarly several vascularisation factors (vascular endothelial growth factor (VEGF), monocyte chemoattractant proteins (MCP-1) and interferon inducible protein 10 (IP-10), was also reduced by 2.25ppm fluoride. ALP activity and proliferation were stimulated by 0.46ppm fluoride but inhibited by 2.25ppm fluoride. CONCLUSIONS: These results indicate that fluoride may impact on the expression of structural enamel proteins and the protease responsible for processing these proteins during the secretory stage of amelogenesis and go some way to explaining the mineralization defect that characterises fluorotic enamel.

• Riksen, Elisabeth Aurstad; Stunes, Astrid Kamilla; Kalvik, Anne; Gustafsson, Björn; Snead, Malcolm & Syversen, Unni [Vis alle 8 forfattere av denne artikkelen] (2010). Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells. European Journal of Oral Sciences. ISSN 0909-8836. 118(6), s. 566–573. doi: 10.1111/j.1600-0722.2010.00778.x.

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• Riksen, Elisabeth Aurstad (2024). KURS I SEDASJON MED LYSTGASS OG BENZODIAZEPINER.
• Riksen, Elisabeth Aurstad (2023). Kurs i sedasjon med lystgass og legemidler.
• Riksen, Elisabeth Aurstad (2011). Effects of epigenetic and environmental factors on biomineralization.
• Riksen, Elisabeth Aurstad; Petzold, Christiane; Brookes, Steven; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2011). Human Osteoblastic Cells Discriminate Between 20kDa Amelogenin-isoforms.
• Riksen, Elisabeth Aurstad (2010). Effekt av ameloblastin og amelogenin på osteoblaster på titan overflater både in vitro og in vivo.
• Riksen, Elisabeth Aurstad (2009). Fluoride reduces the expression of enamel proteins and vascular growth factors in ameloblasts in vitro”.
• Riksen, Elisabeth Aurstad (2008). Effects of temperature and fluoride on ameloblasts in vitro.
• Riksen, Elisabeth Aurstad (2008). ” Effect of enamel matrix proteins on mineralizing tissue”.
• Riksen, Elisabeth Aurstad (2008). ”Effekt av Serotonin-reopptakshemmere på ameloblaster”.
• Riksen, Elisabeth Aurstad (2008). “Effects of epigenetic and environmental factors on biomineralization”.
• Riksen, Elisabeth Aurstad (2008). Effekt av SSRI medikamenter på ameloblaster”.
• Riksen, Elisabeth Aurstad; Ellingsen, Jan Eirik; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2008). In vivo and in vitro effects of enamel matrix proteins on osteoblasts.
• Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Ellingsen, Jan Eirik; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2007). Comparing the effects of emdogain (EMD), amelogenin (AMEL) and ameloblastin (AMBN) on bone growth onto titanium implants. Calcified Tissue International. ISSN 0171-967X. 80.
• Kalvik, Anne; Riksen, Elisabeth Aurstad; Hynne, Astrid; Lian, Aina-Mari; Kvam, Britt-Mari & Lyngstadaas, Ståle Petter [Vis alle 7 forfattere av denne artikkelen] (2007). Effect of temperature and fluoride on ameloblasts in vitro. Calcified Tissue International. ISSN 0171-967X. 80.
• Kalvik, Anne; Riksen, Elisabeth Aurstad; Hynne, Astrid; Reseland, Janne Elin & Lyngstadaas, Ståle Petter (2007). Effect of temperature and fluoride on ameloblasts in vitro.
• Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Ellingsen, Jan Eirik; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2007). Comparing the effects of Emdogain (EMD), amelogenin (AMEL) and ameloblastin (AMBN) on bone growth onto titanium implants.
• Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Ellingsen, Jan Eirik; Lyngstadaas, Ståle Petter & Reseland, Janne Elin (2005). Comparing the effects of EMD, amelogenin and amelin on titanium implant attachment in rabbit tibia bone and osteoblasts in vitro.
• Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Reseland, Janne Elin; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2004). �Effects of different amelogenin-peptides on Saos and OHS osteoblasts.�. Vis sammendrag

  Objectives In the developing enamel matrix amelogenins are the main component, comprising approximately 90 % of the proteins secreted by the ameloblast cells. Amelogenins in the form of EmdogainÒ (Biora, Malmø, Sweden) EMD, a commercially available formulation of porcine proteins in polyglycol alginate (PGA), is currently successfully used to regain alveolar bone after degenerative periodontitis. The main component of EMD, are two 23 kD and 25 kD molecules that are immunoreactive with polyclonal anti-amelogenin antibodies. The aim of this study was to test the two ~20 kD molecules A1 and A2 on P5 generation cell cultures of SaOs and OHS human osteosarcoma osteoblasts to investigate proliferation and differentiation in in vitro assays. Experimental methods SaOs P3, OHS P3 is taken from liquid nitrogen and cultivated to confluence and seeded on 96 (proliferation) and 6 (differentiation) well plates. Concentration of test medium was 2mg/ml, 10mg/ml and 50 mg/ml of A1, A2 or EMD, and medium was used as control. All the tests were done on P5 generation in parallels, twice when confluent. Medium was harvested and cells lysated after 24h, 48h, 72h and 1 week and proteins and mRNA from the cells and proteins from the media were analyzed for Alkaline phosphatase activity (ALP), CD44, Osteocalcin (OC), lactate dehydrogenase activity (LDH), Cbfa-1 and IL-6 using ELISA and PCR. Results The results demonstrated that the osteoblastic cells responded differently to the two amelogenin-like proteins. Exposure to A1 resulted in a persistent doubling of ALP activity when compared to the control whereas A2 did not cause any significant change in this parameter. Moreover, A2 caused a significant increase in CD44 expression after 7 day in culture. CD44 expression was not observed in cells cultured with A1. The opposite effect was observed for osteoblast specific expression factor Cbfa-1 where A1 caused a significant increase whereas A2 caused a decrease. Both A1 and A2 caused significant increases in IL-6 expression; A2 gave a six-fold increase whereas A1 caused a doubling of the IL-6 expression. Conclusions The results suggest that both A1 and A2 participate in the observed effect of EMD. However, A1 appears to help in maintaining an osteblastic phenotype, with an up-regulation of Cbfa-1 and IL-6. A2 on the other hand might facilitate differentiation of osteoblastic cells into a more ostecyte-like phenotype with increased CD44 expression and a decrease in Cbfa-1. Alternatively, it can be speculated that the two molecules are recognized differently by two different subpopulations of the cultured osteoblasts. Further investigations are needed to elucidate if the combination of A1 and A2 is more efficient for bone and periodontal ligament regeneration than either of the components on their own. Moreover, a full sequence analysis is needed to characterize the molecules and further identify their role in bone growth, enamel formation and tooth development. EU Grant QLK3-CT-2001-00090 supported this study.

• Riksen, Elisabeth Aurstad; Reseland, Janne Elin; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2004). �Human Osteoblastic Cells Discriminate Between Amelogenins of Different Size�.
• Riksen, Elisabeth Aurstad; Reseland, Janne Elin; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2004). �Human Osteoblastic Cells Discriminate Between Amelogenin-like Proteins of Similar Size�.
• Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Reseland, Janne Elin & Lyngstadaas, Ståle Petter (2004). Effect of fractionated Enamel Matrix Derivate on SaOs and OHS osteosarcoma cells.
• Berntsen, Christian Skoe; Riksen, Elisabeth Aurstad; Bemer, Heidi Sire; Reseland, Janne Elin; Slaby, I & Dutch, D [Vis alle 8 forfattere av denne artikkelen] (2003). Effects of extracellular matrix proteins on bone differentiation around titan implants. Journal of Bone and Mineral Research. ISSN 0884-0431. 18, s. S229–S229.
• Riksen, Elisabeth Aurstad; Berntsen, Christian Skoe; Ellingsen, Jan Eirik; Deutsch, Dan; Rønold, Hans Jacob & Lyngstadaas, Ståle Petter (2003). The Effects of EMD and rhAmelogenin on Titanium Implant Attachment in Cortical Bone.
• Berntsen, Christian Skoe; Riksen, Elisabeth Aurstad; Ellingsen, Jan Eirik; Slaby, Ivan; Rønold, Hans Jacob & Lyngstadaas, Ståle Petter (2003). Improved Titanium Implant Attachment in Cortical Bone by Application of rAmelin.
• Berntsen, Christian Skoe; Riksen, Elisabeth Aurstad; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2003). �Improved Titanium Implant Attachment in Cortical Bone by Application of rAmelin�.
• Riksen, Elisabeth Aurstad; Rønold, Hans Jacob; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2003). �The effect of EMD and rAmelogenin on titanium implant attachment in cortical bone�.
• Bernsten, Christian Skoe; Riksen, Elisabeth Aurstad; Berner, Heidi Sire; Reseland, Janne Elin; Slaby, Ivan & Deutsch, Dan [Vis alle 8 forfattere av denne artikkelen] (2003). Effects of extracellular matrix proteins on bone differentiation around titanium implants.
• Riksen, Elisabeth Aurstad; Rønold, Hans Jacob & Ellingsen, Jan Eirik (2002). �An in vivo method for measuring bone attachment of bone- anchored implants�.
• Riksen, Elisabeth Aurstad; Berntsen, Christian Skoe; Rønold, Hans Jacob; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2002). An in vivo method for measuring extracellular matrix guided osseointegration.
• Berntsen, Christian Skoe; Riksen, Elisabeth Aurstad; Rønold, Hans Jacob; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2002). Molecular force probing of amelogenin-titanium interactions.
• Riksen, Elisabeth Aurstad; Berntsen, Christian Skoe; Ellingsen, Jan Eirik & Lyngstadaas, Ståle Petter (2002). An in-vivo method for measured extracellular matrix guided osseointegration.
• Riksen, Elisabeth Aurstad (2011). Effects of epigenetic and environmental factors on biomineralization. Unipub forlag. ISSN 978-82-91757-64-3.

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