Qianli Ma

• Øvrebø, Øystein; De Lauretis, Angela; Ma, Qianli; Perale, Giuseppe; Nilsen, Ola & Rossi, Filippo [Show all 7 contributors for this article] (2024). Towards bone regeneration: Understanding the nucleating ability of proline-rich peptides in biomineralisation. Biomaterials Advances. ISSN 2772-9516. 159. doi: 10.1016/j.bioadv.2024.213801. Full text in Research Archive
• Goa, Hui; Jiang, Nan; Niu, Qiannan; Mei, Shenglin; Haugen, Håvard Jostein & Ma, Qianli (2023). Biocompatible nanostructured silver-incorporated implant surfaces show effective antibacterial, osteogenic, and anti-inflammatory effects in vitro and in rat model. International Journal of Nanomedicine. ISSN 1176-9114. 18, p. 7359–7378. doi: 10.2147/IJN.S435415. Full text in Research Archive
• Miri, Zahra; Haugen, Håvard Jostein; Loča, Dagnija; Rossi, Filippo; Perale, Giuseppe & Moghanian, Amirhossein [Show all 7 contributors for this article] (2023). Review on the strategies to improve the mechanical strength of highly porous bone bioceramic scaffolds. Journal of the European Ceramic Society. ISSN 0955-2219. doi: 10.1016/j.jeurceramsoc.2023.09.003. Full text in Research Archive
• Han, Jingyuan; Ma, Qianli; An, Yanxin; Wu, Fan; Zhao, Yuqing & Wu, Gaoyi [Show all 7 contributors for this article] (2023). The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces. Journal of Nanobiotechnology. ISSN 1477-3155. 21(1), p. 277–306. doi: 10.1186/s12951-023-02017-8. Full text in Research Archive
• Miri, Zahra; Farè, Silvia; Ma, Qianli & Haugen, Håvard Jostein (2023). Updates on polyurethane and its multifunctional applications in biomedical engineering. Progress in Biomedical Engineering. 5(4). doi: 10.1088/2516-1091/acef84. Full text in Research Archive
• Ødegaard, Kristin Sirnes; Westhrin, Marita; Bin Afif, Abdulla Shaikh Abdul Qader; Ma, Qianli; Mela, Petra & Standal, Therese [Show all 8 contributors for this article] (2023). The effects of surface treatments on electron beam melted Ti-6Al-4V disks on osteogenesis of human mesenchymal stromal cells. Biomaterials Advances. ISSN 2772-9516. 147. doi: 10.1016/j.bioadv.2023.213327.
• Ma, Qianli; Miri, Zahra; Haugen, Håvard Jostein; Moghanian, Amirhossein & Loca, Dagnija (2023). Significance of mechanical loading in bone fracture healing, bone regeneration, and vascularization. Journal of Tissue Engineering. ISSN 2041-7314. 14. doi: 10.1177/20417314231172573. Full text in Research Archive Show summary

  In 1892, J.L. Wolff proposed that bone could respond to mechanical and biophysical stimuli as a dynamic organ. This theory presents a unique opportunity for investigations on bone and its potential to aid in tissue repair. Routine activities such as exercise or machinery application can exert mechanical loads on bone. Previous research has demonstrated that mechanical loading can affect the differentiation and development of mesenchymal tissue. However, the extent to which mechanical stimulation can help repair or generate bone tissue and the related mechanisms remain unclear. Four key cell types in bone tissue, including osteoblasts, osteoclasts, bone lining cells, and osteocytes, play critical roles in responding to mechanical stimuli, while other cell lineages such as myocytes, platelets, fibroblasts, endothelial cells, and chondrocytes also exhibit mechanosensitivity. Mechanical loading can regulate the biological functions of bone tissue through the mechanosensor of bone cells intraosseously, making it a potential target for fracture healing and bone regeneration. This review aims to clarify these issues and explain bone remodeling, structure dynamics, and mechano-transduction processes in response to mechanical loading. Loading of different magnitudes, frequencies, and types, such as dynamic versus static loads, are analyzed to determine the effects of mechanical stimulation on bone tissue structure and cellular function. Finally, the importance of vascularization in nutrient supply for bone healing and regeneration was further discussed

• Ma, Qianli; Rubenis, Kristaps; Sigurjonsson, Olafur E.; Hildebrand, Torben; Standal, Therese & Zemjane, Signe [Show all 9 contributors for this article] (2023). Eggshell-derived amorphous calcium phosphate: Synthesis, characterization and bio-functions as bone graft materials in novel 3D osteoblastic spheroids model. Smart Materials in Medicine. ISSN 2590-1834. 4, p. 522–537. doi: 10.1016/j.smaim.2023.04.001. Full text in Research Archive
• Fu, Zhaoyue; Hou, Yongli; Haugen, Håvard Jostein; Chen, Xutao; Tang, Kang & Fang, Liang [Show all 10 contributors for this article] (2023). TiO2 nanostructured implant surface-mediated M2c polarization of inflammatory monocyte requiring intact cytoskeleton rearrangement. Journal of Nanobiotechnology. ISSN 1477-3155. 21. doi: 10.1186/s12951-022-01751-9. Full text in Research Archive Show summary

  Background Microgravity directly disturbs the reorganization of the cytoskeleton, exerting profound effects on the physiological process of macrophages. Although it has been established that macrophage M1/M2 polarization could be manipulated by the surface nanostructure of biomaterial in our previous study under normal gravity, how will inflammatory monocytes (iMos)-derived macrophages respond to diverse nanostructured Ti surfaces under normal gravity or microgravity remains unrevealed. Results In this study, Cytochalasin D, a cytoskeleton relaxant, was employed to establish the simulated microgravity (SMG) environment. Our results showed that human iMos polarized into M2c macrophages on NT5 surface but M1 type on NT20 surface with divergent inflammatory phenotypes according to the profile of macrophage polarization featured molecules under normal gravity. However, such manipulative effects of NTs surfaces on iMos-derived macrophages were strikingly weakened by SMG, characterized by the altered macrophage morphology, changed cytokine secretion profile, and decreased cell polarization capacity. Conclusions To our knowledge, this is the first metallic implantable material study focusing on the functions of specific monocyte subsets and its crucial role of the cytoskeleton in materials-mediated host immune response, which enriches our mechanism knowledge about the crosstalk between immunocytes and biomaterials. The results obtained in the present study may also provide potential targets and strategies for biomaterial development and clinical treatment via precise immune-regulation under normal gravity and microgravity.

• He, Yide; Gao, Yuanxue; Ma, Qianli; Zhang, Xige; Zhang, Yumei & Song, Wen (2022). Nanotopographical cues for regulation of macrophages and osteoclasts: emerging opportunities for osseointegration. Journal of Nanobiotechnology. ISSN 1477-3155. 20(1). doi: 10.1186/s12951-022-01721-1. Full text in Research Archive
• Kohtala, Sampsa; Nedal, Tonje Marie Vikene; Kriesi, Carlo Rene; Moen, Siv Helen; Ma, Qianli & Ødegaard, Kristin Sirnes [Show all 8 contributors for this article] (2022). Automated Quantification of Human Osteoclasts Using Object Detection. Frontiers in Cell and Developmental Biology. ISSN 2296-634X. 10(941542). doi: 10.3389/fcell.2022.941542. Full text in Research Archive Show summary

  A balanced skeletal remodeling process is paramount to staying healthy. The remodeling process can be studied by analyzing osteoclasts differentiated in vitro from mononuclear cells isolated from peripheral blood or from buffy coats. Osteoclasts are highly specialized, multinucleated cells that break down bone tissue. Identifying and correctly quantifying osteoclasts in culture are usually done by trained personnel using light microscopy, which is time-consuming and susceptible to operator biases. Using machine learning with 307 different well images from seven human PBMC donors containing a total of 94,974 marked osteoclasts, we present an efficient and reliable method to quantify human osteoclasts from microscopic images. An open-source, deep learning-based object detection framework called Darknet (YOLOv4) was used to train and test several models to analyze the applicability and generalizability of the proposed method. The trained model achieved a mean average precision of 85.26% with a correlation coefficient of 0.99 with human annotators on an independent test set and counted on average 2.1% more osteoclasts per culture than the humans. Additionally, the trained models agreed more than two independent human annotators, supporting a more reliable and less biased approach to quantifying osteoclasts while saving time and resources. We invite interested researchers to test their datasets on our models to further strengthen and validate the results.

• Aass, Kristin Roseth; Mjelle, Robin; Kastnes, Martin Haugrud; Tryggestad, Synne Stokke; van den Brink, Luca Mare & Roseth, Ingrid Aass [Show all 19 contributors for this article] (2021). Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells. iScience. ISSN 2589-0042. 25(1). doi: 10.1016/j.isci.2021.103605. Full text in Research Archive Show summary

  Interleukin-32 (IL-32) is a nonclassical cytokine expressed in cancers, inflammatory diseases, and infections. Its expression is regulated by two different oxygen sensing systems; HIF1α and cysteamine dioxygenase (ADO), indicating that IL-32 may be involved in the response to hypoxia. We here demonstrate that endogenously expressed, intracellular IL-32 interacts with components of the mitochondrial respiratory chain and promotes oxidative phosphorylation. Knocking out IL-32 in three myeloma cell lines reduced cell survival and proliferation in vitro and in vivo. High-throughput transcriptomic and MS-metabolomic profiling of IL-32 KO cells revealed that cells depleted of IL-32 had perturbations in metabolic pathways, with accumulation of lipids, pyruvate precursors, and citrate. IL-32 was expressed in a subgroup of myeloma patients with inferior survival, and primary myeloma cells expressing IL-32 had a gene signature associated with immaturity, proliferation, and oxidative phosphorylation. In conclusion, we demonstrate a previously unrecognized role of IL-32 in the regulation of plasma cell metabolism.

• Ødegaard, Kristin Sirnes; Ouyang, Lingzi; Ma, Qianli; Buene, Glenn; Wan, Di & Elverum, Christer Westum [Show all 9 contributors for this article] (2021). Revealing the influence of electron beam melted Ti-6Al-4V scaffolds on osteogenesis of human bone marrow-derived mesenchymal stromal cells. Journal of materials science. Materials in medicine. ISSN 0957-4530. 32(97). doi: 10.1007/s10856-021-06572-0. Full text in Research Archive Show summary

  Porous Titanium-6Aluminum-4Vanadium scaffolds made by electron beam-based additive manufacturing (AM) have emerged as state-of-the-art implant devices. However, there is still limited knowledge on how they influence the osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs). In this study, BMSCs are cultured on such porous scaffolds to determine how the scaffolds influence the osteogenic differentiation of the cells. The scaffolds are biocompatible, as revealed by the increasing cell viability. Cells are evenly distributed on the scaffolds after 3 days of culturing followed by an increase in bone matrix development after 21 days of culturing. qPCR analysis provides insight into the cells’ osteogenic differentiation, where RUNX2 expression indicate the onset of differentiation towards osteoblasts. The COL1A1 expression suggests that the differentiated osteoblasts can produce the osteoid. Alkaline phosphatase staining indicates an onset of mineralization at day 7 in OM. The even deposits of calcium at day 21 further supports a successful bone mineralization. This work shines light on the interplay between AM Ti64 scaffolds and bone growth, which may ultimately lead to a new way of creating long lasting bone implants with fast recovery times.

• Ma, Qianli; Jiang, Nan; Shuang, Liang; Fulin, Chen; Liang, Fang & Xian, Wang [Show all 8 contributors for this article] (2020). Functionalization of a clustered TiO2 nanotubular surface with platelet derived growth factor-BB covalent modification enhances osteogenic differentiation of bone marrow mesenchymal stem cells. Biomaterials. ISSN 0142-9612. 230, p. 119650–119650. doi: 10.1016/j.biomaterials.2019.119650.
• Ma, Qianli; Jiang, Nan; Liang, Shuang; Chen, Fulin; Fang, Liang & Wang, Xian [Show all 8 contributors for this article] (2019). Functionalization of a clustered TiO2 nanotubular surface with platelet derived growth factor-BB covalent modification enhances osteogenic differentiation of bone marrow mesenchymal stem cells. Biomaterials. ISSN 0142-9612. 230(119650). doi: 10.1016/j.biomaterials.2019.119650.

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• Han, Jingyuan; Ma, Qianli; An, Yanxin; Wu, Fan; Zhao, Yuqing & Wu, Gaoyi [Show all 7 contributors for this article] (2023). Correction: The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces (Journal of Nanobiotechnology, (2023), 21, 1, (277), 10.1186/s12951-023-02017-8). Journal of Nanobiotechnology. ISSN 1477-3155. 21(1). doi: 10.1186/s12951-023-02102-y.
• Alam, Anam; Haugen, Håvard Jostein; Ma, Qianli & Loča, Dagnija (2023). USING EGGSHELLS FOR BONE GRAFTS. Springwise. Show summary

  Bone grafts are considered to be the gold standard in terms of repairing and reconstructing damaged bones. These can either be autologous, where a patient’s own tissue is used, or allogeneic, where the tissue is taken from another person. But limited supply, donor-site complications, and risk of disease transmission can often prevent allogeneic grafts from being used. Xenogeneic bone grafts, where the graft is taken from another species, represent a feasible alternative. But, because xenograft materials come from mammal tissue, it raises ethical questions surrounding animal welfare. To find a safe, adaptable, and environmentally friendly alternative, a team of researchers has developed a method to convert eggshells into endotoxin-free and immunocompatible amorphous calcium phosphate (ACP) particles. First the shells were heated, then mixed with distilled water and phosphoric acid. The precipitate was then filtered and washed, before being submerged in liquid nitrogen. The resulting ACP particles are essential to forming hard and strong bone and can therefore be used as an ideal bone substitute. As well as containing plenty of calcium and phosphorous, Dr Qianli Ma – the study’s lead author – highlights that eggshells are also an ideal raw material because they contain traces of magnesium and strontium, which are associated with healthy bone regeneration. The team also created a novel 3D spheroid model, which allows the activity of eggshell ACP to be studied in vitro. With the model, the researchers could observe how the ACP materials would actually interact with osteoblasts, and they were found to be safe and effective in promoting bone regeneration. If scaled successfully, this new technique could enable an unlimited supply of sustainable bone graft materials, while reducing the volume of eggshells going to waste. The scientists hope their latest findings inspire additional research into the conversion of food waste into high-value biomaterials. Eggshells are not only being used in the medical field. Springwise has also spotted a design studio creating wall tiles from discarded eggshells, and another that has transformed the waste into car interiors

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