Publications
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Young, Alix; Hove, Lene Hystad; Stevning, Karen; Løvik, Sara-Emilie & Hjortsjö, Carl
(2021).
Kan tannpasta
gjøre tennene mer
sårbare?
En in vitro pilotstudie om endring
i fukting av emalje uttrykt ved
kontaktvinkelmålinger.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
131(9),
p. 796–801.
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Brennhovd, Anders; Petrovski, Beata & Hjortsjö, Carl
(2020).
Benoppbygging
før implantatinnsetting – overlevelse og suksess.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
130(10),
p. 796–805.
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Hjortsjø, Carl
(2018).
Bittskinnebehandling.
In Julsvoll, Elisabeth Heggem (Eds.),
Temporomandibulær dysfunksjon: Teori, undersøkelse og behandling.
Cappelen Damm Akademisk.
ISSN 978-82-02-55779-9.
p. 224–236.
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Printzell, Lisa; Haseid, Carl Fredrik; Ekfeldt, Anders & Hjortsjø, Carl
(2016).
Clinical performance of enamel-dentine bonded all-ceramic restorations: retrospective evaluation in a postgraduate clinic.
Swedish Dental Journal.
ISSN 0347-9994.
40,
p. 203–212.
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Hjortsjø, Carl; Young, Alix; Kiesow, Andreas; Cismak, Andreas; Berthold, Lutz & Petzold, Matthias
(2015).
A Descriptive in vitro Electron Microscopic Study of Acidic Fluoride-Treated Enamel: Potential Anti-Erosion Effects.
Caries Research.
ISSN 0008-6568.
49(6),
p. 618–625.
doi:
10.1159/000441195.
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Hjortsjø, Carl; Mjølnerød, Jol Anders; Skaare, Anders; Jonski, Grazyna; Young, Alix & Ekfeldt, Anders
(2012).
A replica technique for studying the effect of fluoride solutions on enamel erosion.
Swedish Dental Journal.
ISSN 0347-9994.
36 ,
p. 169–178.
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Hjortsjø, Carl; Jonski, Grazyna; Young, Alix & Saxegaard, Erik
(2010).
Effect of acidic fluoride treatments on early enamel erosion lesions-A comparison of calcium and profilometric analyses.
Archives of Oral Biology.
ISSN 0003-9969.
55(3),
p. 229–234.
doi:
10.1016/j.archoralbio.2010.01.003.
Show summary
Objective: The protective effect of fluoride solutions against acidic challenge on enamel was tested in vitro using calcium- and profilometer analyses. Methods: HF-, SnF2-, TiF4- and NaF solutions (0.1M F-) and H2O as negative control were tested on a total of 60 enamel specimens divided into ten groups. Prior to and after F-treatment five groups were exposed for 1min to 0.01M citric acid, and the other five groups for 10min to 0.1M citric acid. Enamel specimens were measured by profilometry at baseline and before and after each acidic challenge. [Ca] in the collected citric acid samples was measured by atom absorption spectroscopy (AAS). ΔCa and Δ surface height were calculated for each specimen and the data were analysed using one way ANOVA, Tukeys test and Pearson r correlation. Results: AAS showed that F-treatments reduced enamel dissolution for both the 1min and 10min acidic challenge. Profilometry showed no significant differences between the F-solutions for the 1min groups. Significant differences could be seen between the fluorides for the 10min groups and there was a large correlation between the profilometric and AAS results. In conclusion, all fluoride solutions reduced enamel dissolution when specimens were exposed to a typical dietary acid. Conclusions: The profilometer was not sensitive enough to measure the effects of the different fluorides against a low acidic challenge as represented by this in vitro experiment. AAS was able to show these differences and for the 10 min acidic challenge there was a good correlation between the results from the two methods.
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Hjortsjø, Carl; Jonski, Grazyna; Thrane, Per Stanley; Saxegaard, Erik & Young, Alix
(2009).
The Effects of Acidic Fluoride Solutions on Early Enamel Erosion in vivo.
Caries Research.
ISSN 0008-6568.
43(2),
p. 126–131.
doi:
10.1159/000209345.
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Hjortsjø, Carl; Saxegaard, Erik; Young, Alix & Dahl, Jon Einar
(2009).
In vivo and in vitro irritation testing of low concentrations of hydrofluoric acid.
Acta Odontologica Scandinavica.
ISSN 0001-6357.
67(6),
p. 360–365.
doi:
10.1080/00016350903117118.
Show summary
Objective. Acidic fluorides are proposed for the treatment of dental erosion. The aim of this study was to examine the irritation properties of dilute hydrofluoric acid (HF) solutions for potential use in the oral cavity. Material and Methods. Hen’s Egg Test - chorioallantoic membrane (HET-CAM): The CAM was accessed by careful dissection through the egg shell (n=36, 6 eggs/test solution) and exposed to 300 µL of the HF solutions (0.05, 0.10, 0.20 and 1.0%) under macroscope examination over 5 min. Mean time-to-coagulation and average irritation score were recorded based on appearance of hemorrhage, coagulation and lysis of the blood vessels in the membrane. Mouse skin test: Sixty male mice were randomly divided into 10 groups of 6 animals (control, 0.05, 0.10, 0.20 and 1.0% HF), shaved on the back, exposed to test solution and sacrificed after 2h or 24h. Skin samples were evaluated by light microscopy, scoring epithelial leukocyte infiltration, vascular congestion, and edema. Results. HET-CAM: 0.05% HF was slightly irritant, 0.1% HF moderately irritant, 0.2% and 1% HF were strongly irritant. 0.1-1% HF solutions were severely eye irritating. Mouse skin test: HF concentration was significantly correlated with tissue response and 24-h exposure to 1% HF-solution showed focal erosion of the epithelium and marked localized subepithelial leukocyte infiltration. Conclusion. The results of these studies suggest that accidental exposure of soft tissues to solutions containing more than 0.2% HF may be harmful.
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Hjortsjø, Carl; Jonski, Grazyna; Thrane, Per Stanley; Saxegaard, Erik & Young, Alix
(2009).
Effect of Stannous Fluoride and Dilute Hydrofluoric Acid on Early Enamel Erosion over Time in vivo.
Caries Research.
ISSN 0008-6568.
43(6),
p. 449–454.
doi:
10.1159/000261415.
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Jokstad, Asbjørn; Meric, Gøkce & Hjortsjø, Carl
(2005).
A systematic review of the scientific documenttation of fixed partial dentures made form fiber-reinforced polymer to replace missing teeth.
International Journal of Prosthodontics.
ISSN 0893-2174.
View all works in Cristin
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Khan, Sadia Nazir; Koldsland, Odd Carsten & Hjortsjö, Carl
(2019).
Bacteriostatic effect of filling materials used in implant-abutment-screw-channel: A pilot study .
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Jokstad, Asbjørn; Bjørnland, Tore; Dahl, Bjørn L.; Hansen, Torbjørn Leif; Hjortsjö, Carl & Heggem Julsvoll, Elisabeth
[Show all 10 contributors for this article]
(2019).
Diagnostiske kriterier for temporomandibulære forstyrrelser (TMD): Vurderingsverktøy: Norwegian. Version 28Apr2019
.
International Network for Orofacial Pain and Related Disorders Methodology.
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Hove, Lene Hystad; Young, Alix; Hjortsjø, Carl; Stevning, Karen & Løvik, Sara Emilie
(2017).
Effect of Whitening Toothpastes on Dental Enamel
In Vitro
.
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Printzell, Lisa; Haseid, Carl Fredrik; Hjortsjø, Carl & Ekfeldt, Anders
(2016).
Kliniskt utfall av adhesivt cementerade helkeramiska restaureringar utförda på en specialistutbildningsklinik.
Den norske tannlegeforenings tidende.
ISSN 0029-2303.
126(11),
p. 882–888.
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Hjortsjø, Carl
(2015).
Bittskinner ved behandling av TMD
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Hjortsjø, Carl; Jonski, Grazyna & Young, Alix
(2015).
Effect of Mouth-rinsing with Two Fluoride Solutions on Enamel Solubility Using an in vivo Experimental Model.
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Hjortsjø, Carl; Young, Alix ; Kiesow, Andreas; Cismak, Andreas; Berthold, Lutz & Petzold, Matthias
(2013).
An electron microscopic study comparing effects of human enamel exposure to SnF2-, TiF4- and NaF-solutions.
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Hjortsjø, Carl; Jonski, Grazyna & Young, Alix
(2013).
A modified in vivo experimental model for evaluating fluoride treatment against initial erosion lesions.
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Hjortsjø, Carl; Jonski, Grazyna; Young, Alix & Saxegaard, Erik
(2011).
Erosive effect of acidic fluoride solutions on enamel in vitro.
Show summary
Acidic fluoride solutions have been shown to exhibit erosion-inhibiting properties on dental enamel. The aim of this in vitro study was to investigate the possible erosive effect of dilute hydrofluoric acid (HF) on enamel. A 0.2% HF solution (0.1 mol/l F-, pH 3.02) was compared with two acidic fluorides containing 0.5 mol/l F- (3.9% SnF2-pH 2.65 and 1.6% TiF4-pH 1.42) and 1.8% citric acid (acidic control, pH 2.27). 24 human third molar enamel specimens were randomly divided into 4 groups (6 specimen/solution). Enamel surfaces were ground flat by wet grinding with a grit 2500 abrasive paper and half of each flat surface was isolated using a A-silicon impression material. The enamel surfaces were exposed to 20ml of the test solutions for ten minutes in plastic containers. The isolation material was removed. and changes in the enamel surfaces were analysed by LED blue light profilometry (λ=470 nm, 50x magnification). The specimen area analysed included both treated and untreated ground enamel, a total area of 254.64 x 190.90 µm2 for each sample. The difference in surface height between treated and untreated enamel was calculated for each specimen and etching depth data were analysed using oneway ANOVA and Tukeys test. The mean etching depths (µm±SD) were 0.14(0.86), -2.00(0.52), -3.25(2.91) and 5.42(1.00) for, HF, SnF2, TiF4 and control respectively. HF had significantly less etching effect than the control, but was significantly more erosive than TiF4 (p<0.05). In conclusion, while both SnF2 and TiF4 treatments resulted in an average deposition on the enamel surface, HF showed an average etching effect close to zero. Further studies are needed to examine the mechanism of the previously described anti-erosive effect of HF.
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Young, Alix ; Hjortsjø, Carl; Berthold, Lutz; Cismak, Andreas & Petzold, Matthias
(2011).
An electron microscopic study of enamel treated with hydrofluoric acid.
Show summary
Previous studies have demonstrated the effectiveness of treating enamel with acidic fluorides as a means of reducing the effects of erosive challenges. The aim of the present in vitro study was to investigate the surface characteristics of dental enamel treated with hydrofluoric acid (HF). Natural surfaces of enamel specimens from extracted human third molars were treated for 10 min with either native solutions of HF (pH 3) or sodium fluoride (NaF, pH 7) containing both 0.1 and 0.2 mol/l F-. Untreated samples functioned as controls. All specimens were examined by SEM/EDX with respect to surface microstructure variation and fluoridation. For selected specimens, samples were prepared by FIB allowing cross-sectional investigations using TEM and nanospot-EDX. Following treatment with HF, SEM/EDX analyses did not show obvious erosive damage but clearly indicated surface fluoridation. Using TEM, an enamel surface microstructure modification to a depth of approximately 200 nm with a clear boundary to non-affected apatite was revealed. The fluoride content in this layer was above 40at% F with a F/Ca ratio of roughly 2 indicating the transformation of surface hydroxyapatite into CaF2-like material. In previous studies, isolated fluoride-rich globular precipitates were found if medium-acidulated solutions of NaF were applied. The atomic composition of the HF-induced CaF2-like material resembled that of these precipitates. In contrast, no fluoridation effects were found for the NaF at pH 7 and the controls in this study. These results suggest that the general reaction between fluoride solutions and enamel leading to a formation of CaF2-like material does not significantly depend on the dissociated fluoride cation. On the other hand, the reaction rate for the acidic HF treatments is higher since the amount of dissolved calcium increases with decreasing pH.
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Hjortsjø, Carl; Jonski, Grazyna; Saxegaard, Erik & Young, Alix
(2009).
Profilometric study of the effects of acidic fluoride solutions on early dental erosion.
Show summary
An in-vivo experimental model for dental erosion has been utilised to assess the effects of treating dental enamel with fluorides. This in-vitro study aimed to examine the effects of the same acidic fluoride solutions using profilometry. Crowns of intact, surgically extracted human third molar teeth were cut into 2-4 pieces, washed (deionised water, 50% ethanol then 96% ethanol), embedded in acrylic resin and polished (grit 4000). Nail varnish was applied in a line across the block separating the enamel surface from the acrylic resin surface. Specimens were exposed to 5 ml of 100 mmol/L citric acid for 10 min before (etch I) and after (etch II) fluoride treatments. Aqueous solutions of TiF4, SnF2, HF or NaF, (all 0.1 M F-) and deionised water (negative control) were tested (n=6 specimens/test solution). All solutions were applied in a cell culture well (Nunclon, Nunc, Roskilde, Denmark) and enamel specimens were exposed to all the fluids in these wells. Specimens were analysed at all stages in a high power LED blue light profilometer (PLµ2300 Optical Imaging Profiler, Sensofar Tech, Spain) at 150x magnification and λ=470 nm. Areas including one half of exposed enamel and the other half varnish-covered enamel (220.98 x 165.68µm) were analysed to a depth of 40µm. Data analysis compared the step-height differences between the exposed and the varnished reference areas. Paired t-test and Kruskal-Wallis ANOVA on Ranks were used in the statistical analysis. There was a significant reduction in step height difference following HF-, SnF2-, and TiF4-treatments (p ≤0.005), but not for the NaF and water. The acidic fluoride solutions were capable of reducing the erosive effect of the citric acid challenge as previously shown in the in vivo experimental model.
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Young, Alix ; Hjortsjø, Carl; Saxegaard, Erik & Jonski, Grazyna
(2009).
A study on the effect of age on enamel dissolution using a dental erosion model.
Show summary
The prevailing expert opinion suggests that there is little evidence to support the concept that pathological levels of dental erosion are age-dependant. However, some evidence seems to indicate that normal levels of erosion may be age-dependant. Many factors may contribute to this type of wear and presumably the enamel solubility is one of these factors. An in-vivo experimental model for dental erosion using volunteers of various ages has been utilised to assess the effects of treating dental enamel with fluorides to reduce damage due to erosion. The aim of the present study was to examine the possible relationship between the age of the test individuals and the degree of enamel dissolution upon acidic challenge. The in-vivo model has been described previously. In 176 test subjects (age range 18 - 69 years), labial surfaces of 342 healthy anterior teeth were exposed to 5 ml 0.18% citric acid, at 6 ml/min using a peristaltic pump. The acid was collected in coded test tubes for blind analysis of calcium content using atom absorption spectrometry. Mean calcium concentration was calculated separately for central incisors (0.924 mg/L, range 0.033-4.434) and lateral incisors (0.654 mg/L, range 0.051-3.048). The age of the subjects was not normally distributed with a mean age of 27.8 years. Statistical analysis using Spearman’s rho showed no correlation between the age of the test subjects and the calcium concentration in the acid etch (central incisors rs = 0.048, lateral incisors rs = 0.161, p<0.05). Despite the skewed age distribution these results do not support a correlation between age and degree of enamel dissolution following a brief acidic challenge.
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Hjortsjø, Carl; Jonski, Grazyna; Saxegaard, Erik & Young, Alix
(2009).
Validation of a dental erosion model I–chemical analysis in-vitro.
Show summary
An in vivo experimental model for dental erosion has been utilised to assess the effects of treating dental enamel with fluorides as a preventive and/or treatment measure. Objective: To validate the in-vivo experimental model results in-vitro using chemical analysis. Methods: Crowns of intact, surgically extracted human third molar teeth were cut into 2-4 pieces, washed (deionised water, 50% ethanol, 96% ethanol), embedded in acrylic resin and polished (grit 4000). Specimens were exposed to 5 ml of 10 mmol/L citric acid for 1 min before (etch I) and after (etch II) fluoride treatment. Aqueous solutions of TiF4, SnF2, HF and NaF (all 0.1 M F-) and deionised water (negative control) were tested (n = 6 tooth specimens/test solution). The citric acid and test solutions were applied using a peristaltic pump (flow: 6 ml/min, Gilson model Minipuls 3). Etch and fluoride solutions were collected in coded tubes and analysed blind for calcium content using atomic absorption spectrometry (Model 3300, Perkin Elmer Analytical Instruments, USA) using air-acetylene flame and λ = 422.7 nm. ∆ ppm calcium (etch I minus etch II) was calculated for each tooth and mean percentage reduction in calcium loss was determined for each test solution. Results were analysed using one way ANOVA and the Bonferroni t-test for multiple comparisons. Results: The HF- and SnF2-treatments were significantly more effective in reducing calcium dissolution than the TiF4 and NaF-treatments (p≤0.004). There was no statistical difference between TiF4 and NaF- treatments (p=0.138). The water control resulted in an increase in calcium dissolution. Conclusion: These results correlate well with results from the in vivo model and help validate the use of calcium analysis in the in-vivo dental erosion model.
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Hjortsjø, Carl; Dahl, Jon Einar; Saxegaard, Erik & Young, Alix
(2008).
HET-CAM testing of diluted hydrofluoric acid solutions.
Show summary
It has been suggested that diluted hydrofluoric acid (HF) solutions (≤ 0.2%) can reduce enamel dissolution in a clinical dental erosion model. The toxic effect of concentrated solutions of HF is well known. The possible effects of low concentrations of HF are less well known. This study aimed to examine the irritative effects of four HF solutions (1%, 0.2%, 0.1% and 0.05%) using the Hen’s Egg Test - chorioallantoic membrane (HET-CAM) (Kalweit et al., Toxicol in Vitro 1990;4:702-706). The hypothesis to be tested was that low concentrations of HF do not provoke any response on the blood vessels of the CAM of fertilized hens’ eggs. The CAM was accessed by careful dissection through the egg shell. 300 μl of the test solutions was dripped on the CAM under constant examination using a macroscope. The time for appearance of haemorrhage, vessel lysis and coagulation in the membrane was recorded. An average irritation score (IS) for each test solution (n=24 eggs in total) was calculated and a score was assigned: 0-0.9 = non irritant, 1-4.9 = slight irritant, 5-8.9 = moderate irritant and 9-21 = strong irritant. Mean time to coagulation (mtc100) was also calculated, values below 100 were classified as R41 - severely eye irritating. Results showed that 0.05% HF scored as slightly irritant (IS=3.26), 0.1% HF as moderately irritant (IS=7.49) while both 0.2% and 1% HF were recorded as strongly irritant (IS=9.92 and 12.22 respectively). 0.1%, 0.2% and 1% HF were classified as severe eye irritants (mtc100= 75, 80 and 50). In conclusion all four HF solutions provoked reactions on the chorioallantoic membrane of fertilized hen’s eggs. Care must therefore be taken when using diluted HF solutions under experimental clinical conditions.
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Hjortsjø, Carl; Jonski, Grazyna; Rølla, Gunnar; Thrane, Per Stanley; Saxegaard, Erik & Young, Alix
(2008).
Short term effect of NaF-, SnF2- and dilute HF-treatments on enamel erosion-like lesions in vivo.
Show summary
Dental erosion is a clinical problem in many countries today partly due to increased exposure to dietary acids. Citric acid for example is commonly found in fruit juices, mineral water, flavoured teas and bottled still water. Recent studies have shown that several acidic fluoride preparations hold promise in the prevention and treatment of dental erosion. This study aimed at comparing the effect of sodium fluoride (NaF, pH 7.3), stannous fluoride (SnF2, pH 2.9) and dilute hydrofluoric acid (HF, pH 2.0) solutions, on enamel dissolution in an experimental in vivo model as described previously (Young et al., Eur J Oral Sci 2006;114;180-183). Four healthy anterior teeth in each subject (n=29) were isolated using plastic strips and a light bodied impression material for complete palatal coverage. The labial surfaces of the test teeth were exposed to 5 ml citric acid (0.18%) using a peristaltic pump (6 ml/min), before (etch I) and 5 min after (etch II) application of test fluoride preparations. The acid was collected in a test tube for later analysis. NaF, SnF2 or HF solutions (all 0.1 M F-) were applied to the labial surfaces of the teeth for 1 min using a peristaltic pump (6 ml/min). Enamel dissolution was examined by measuring calcium content in the citric acid using atom absorption spectrometry. Mean values for etch I minus etch II were calculated and statistical analysis with the Bonferroni t-test for between group comparisons. HF and SnF2 gave mean calcium reductions of 0.37 ppm (76%) and 0.67 ppm (68%) respectively (NS). NaF gave no calcium reduction (-0.34 ppm (-52%). In conclusion, while neutral NaF had no protective effect 5 min after treatment, SnF2 and dilute HF markedly reduced calcium loss.
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Hjortsjø, Carl; Jonski, Grazyna; Saxegaard, Erik; Thrane, Per Stanley & Young, Alix
(2007).
Effect of time on dilute hydrofluoric acid-treatments of enamel in vivo.
Show summary
Dental erosion is currently considered to be one of the main reasons for tooth wear. It has been suggested in an in vivo study that hydrofluoric acid (HF) successfully reduces enamel dissolution and protects enamel against acidic challenges.
The aim of this study was to evaluate the protective effect of 0.2% HF solution over time, using a newly established indirect experimental in vivo model. Test subjects (n=28) took part in this study. Healthy anterior teeth (n=107) with intact pellicle were kept dry during the experiment using cotton rolls and saliva suction, and isolated using plastic strips and a light-bodied impression material for complete coverage of palatinal surfaces. The labial surfaces were exposed to diluted citric acid (10mmol/l) using a peristaltic pump (5ml, 6ml/min). The acid was collected in a test tube before (etch I) and after 24 h (n=54 teeth) or 7 days (n=53 teeth) (etch II), following application of aqueous 0.2% HF solution (6ml, 6ml/min). The teeth were rinsed for 5s with water after both etch I and II. Enamel dissolution was examined by assessment of calcium content in the citric acid of etch I and II using atom absorption spectroscopy. The reductions in calcium content in the citric acid between etch I and etch II were calculated for each tooth (p< 0.05). A significant reduction in post HF-treatment calcium loss was observed for both time periods, though the protective effect decreased with time. Mean percent calcium loss reduction was 49 (S.E. 3.6) for the 24-h group and 29 (S.E. 5.4) for the 7-d group. The results suggest that 0.2% HF has a protective effect on sound enamel that lasts for at least one week.
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Young, Alix ; Thrane, Per Stanley; Saxegaard, Erik; Hjortsjø, Carl; Jonski, Grazyna & Rølla, Gunnar
(2006).
Preventive effect of acidic fluoride treatments on erosion-like lesions.
Show summary
Dental erosion is an increasingly important clinical problem and treatment of serious cases often both difficult and expensive. Prevention and treatment involving fluoride application in various forms has been studied both in vitro and in situ. Objectives: To compare the effect of acidic fluoride against neutral fluoride on erosion-like lesions using an in vivo model. Methods: Four healthy anterior teeth in each subject (n=40) with intact pellicle were individually exposed to citric acid (100mmol/l or 10mmol/l) applied using a peristaltic pump (5ml, 7ml/min) and the acid collected in test tubes before (etch I) and after (etch II) applications of the respective fluoride preparations. Fluoride toothpastes (0.15% NaF and 0.4% SnF2) were applied to the labial surfaces with a soft brush (4 x 1min) with gentle water rinsing between applications. Fluoride solutions (0.15% HF, pH 2.5 and 0.35% NaF, pH 8.8) were dripped continuously on the labial surfaces of each tooth (1 min, 4 ml/min), followed by 5 s water rinse/tooth. Subjects rested 1 min prior to etch II. Enamel solubility was examined by assessment of calcium content (Ca) in etch I and II using atom absorption spectroscopy. Results: Toothpastes – SnF2 provided far better protection of the enamel than NaF reducing Ca-loss by 20% when the weakest acid was used. NaF resulted in large Ca-loss with both acid concentrations. Solutions – HF reduced Ca-loss by 60 % whereas the NaF resulted in large Ca-loss with both acid concentrations. Conclusions: The experiments showed that SnF2 and highly dilute HF are promising preventive treatment measures for dental erosion.
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Young, Alix ; Thrane, Per Stanley; Saxegaard, Erik; Hjortsjø, Carl; Jonski, Grazyna & Rølla, Gunnar
(2006).
Preventive effects of acidic fluoride treatments on erosion-like lesions.
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Saxegaard, Erik; Young, Alix ; Hjortsjø, Carl; Jonski, Grazyna & Rølla, Gunnar
(2006).
Dentin pre-treatment with diluted hydrofluoric acid reduces solubility.
Show summary
We have previously reported that enamel and cementum are protected against dissolution in citric acid by brief pre-treatments with highly diluted hydrofluoric acid (HF, pH=3). (Saxegaard et al IADR 2005). Deposition of calcium fluoride-like material (hereafter CaF2) was shown to account for the effect which was also concentration dependent. However, advanced cases of dental erosion may penetrate the enamel and reach the dentin. Objective: To examine the effect of similar pre-treatments on the solubility of dental dentin. Methods: In 42 intact human third molars the dentin was exposed by cutting off the tooth-enamel using a diamond bur. The roots were cut off and the exposed cervical surface covered with acid-resistant nail varnish. In 6 groups each specimen was treated with 0.1M citric acid (10ml, 30min) before (etch-I) and after (etch-II) treatment with the different HF-solutions ((0.1%-1.0%, 10ml, 10min). Samples were briefly rinsed in deionised water between treatments. Dissolution of dentin was assessed by comparing the calcium content in etch I and II using atomic absorption spectroscopy. Remaining dentin samples similarly underwent etch I and various HF-treatments before examination in EDXA and SEM (Phillips type XL30 ESEM).
Results: Treatment with 0.1%HF reduced the solubility of dentin by ~80%, whereas 0.2-1.0%HF gave reductions of 93-98%. This reduction was somewhat lower than for cementum but higher than for enamel. SEM images showed that dentin was covered with a CaF2, whereas results of EDXA analyses were inconsistent. Conclusion: As with enamel and cementum the CaF2 produced on dentin in the presence of HF appears to exert a protective effect against clinical erosion. It can be speculated that the effect may be due to the low pH at which the CaF2 was formed. It seems likely that the nature of the phosphate contamination of the CaF2 determines its rate of dissolution in erosive acids.
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Hjortsjø, Carl; Jonski, Grazyna; Thrane, Per Stanley; Rølla, Gunnar & Young, Alix
(2006).
Effect of dilute hydrofluoric acid-treatments on enamel in vivo.
Show summary
Dilute hydrofluoric acid (HF) solutions have in earlier tests been proven to reduce enamel solubility. Objectives: To examine the effect of different concentrations of dilute HF in reducing enamel solubility using an in vivo model. Methods: Four healthy anterior teeth in each subject (n=72) with intact pellicle were exposed to diluted citric acid (10mmol/l) applied using a peristaltic pump (5 ml, 6 ml/min) and the acid collected in a test tube before and after application of the respective HF preparations (etch I and etch II). Four different concentrations of HF solutions (0.20%, 0.15%, 0.10%, 0.05%) where dripped on the labial surfaces of each of 18 teeth using the peristaltic pump (1 min, 6 ml/min). The teeth where rinsed for 5s with water between applications and left for five minutes after the treatment with HF before etch II. Enamel solubility was examined by assessment of calcium content in the citric acid applied before and after the treatment with the HF solutions using atom absorption spectroscopy. The reductions in calcium content in the citric acid between etch I and etch II were calculated for each tooth. Results: A greater reduction in calcium loss was observed for the three most concentrated solutions (0.20% HF – 69.7%, 0.15% HF – 61.6%, 0.15% HF – 70.1%) compared to the least concentrated solution (0.05% HF – 41.2%). Conclusions: Even extremely diluted HF seems to provide a very promising level of protection against the effects of acid on enamel solubility. Further studies are planned to determine the clinical relevance of these findings.
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Hjortsjø, Carl
(2010).
Studies on the effect of hydrofluoric acid in prevention of early dental erosion.
Unipub forlag.
ISSN 978-82-91757-57-5.
View all works in Cristin
Published
Nov. 10, 2010 1:46 PM
- Last modified
Nov. 16, 2023 11:50 AM