dariaza

• Stahl, Margrethe Cecilie; Bergendahl, K.G.; Zaytseva-Zotova, Daria; Rytterager, K.R.; Nordgård, Catherine Taylor & Kjesbu, Joachim Sebastian [Vis alle 11 forfattere av denne artikkelen] (2026). RGD-Alginate beads as scaffolds for culturing primary human dermal and pulmonary fibroblasts. Carbohydrate Polymers. ISSN 0144-8617. 379, s. 1–10. doi: 10.1016/j.carbpol.2026.124913. Fulltekst i vitenarkiv Vis sammendrag

  The use of hydrogel microparticles as granular hydrogels is an emerging approach in tissue engineering, where microparticles offer printability, injectability, and serve as scaffolds for cell culture. While culturing dermal fibroblasts for tissue engineering applications is well reported, there is limited research on culturing primary human pulmonary fibroblasts on granular hydrogels. In this study, we grafted RGD-peptides to alginate, formed microbeads, and investigated the ability of granular hydrogels to support the adhesion and growth of primary normal human dermal fibroblasts (NHDFs) and human pulmonary fibroblasts (HPFa). NHDFs adhered to linear RGD (linRGD)-alginate microbeads and spread on the bead surfaces with increased adhesion with increased peptide concentration (0.3–1.3 mM). In contrast, HPFa did not adhere to the linRGD-alginate microbeads. However, HPFa adhered and spread on flat linRGD-alginate gels, indicating that HPFa do respond to linRGD as an adhesion ligand. Supplementation of resulted in cell adhesion to linRGD-alginate microbeads. Enhanced adhesion and spreading of HPFa to RGD-alginate microbeads were observed when using cyclic RGD. Hence, RGD-alginate microbeads is a promising material for structuring primary human dermal and pulmonary fibroblasts, showing the relevance of using alginate microbeads as scaffolds for 3D cultures with fibroblasts.

• Raveendran, Dhanya; Dalloul, Feras; Mietner, J. Benedikt; Barba, Enguerrand; Chen, Shouzheng & Zaytseva-Zotova, Daria [Vis alle 12 forfattere av denne artikkelen] (2025). 3D Printable Hydrogel Based on TEMPO-Oxidized Cellulose Nanofibrils and Fmoc-FF for Enhanced Biological Performance and Cell Adhesion. ACS Applied Nano Materials. 8(38), s. 18571–18583. doi: 10.1021/acsanm.5c03356. Fulltekst i vitenarkiv Vis sammendrag

  Herein, we report a 3D printable ink made of a peptide-polysaccharide hybrid hydrogel composed of fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF) peptide and TEMPO-oxidized cellulose nanofibrils (ToCNF), synthesized using a pH-dependent sol–gel transition method. The ToCNF suspension is synthesized through the mechanical breakdown of a cellulose pulp using a microfluidizer, followed by its oxidation mediated with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). The properties of the hybrid inks are compared in the presence (ToCNF/Fmoc-FF-Ca2+) and absence (ToCNF/Fmoc-FF) of the divalent cation Ca2+, which acts as the cross-linker, at two optimized weight ratios (r) of ToCNF and Fmoc-FF (r = 4.5 and 6.5). The rheological measurements show that the yield strength of the ToCNF/Fmoc-FF-Ca2+ gel is almost double that of the hydrogel composite without Ca2+ ions, especially at the concentration (C) of 10 mM CaCl2. This finding is further verified by 3D gel printing, which produced good quality prints with the cation cross-linked hydrogel. The structural analysis by Field Emission Scanning Electron Microscopy shows that the calcium ions can cross-link the ToCNF and also enhance the self-assembly of Fmoc-FF, which leads to the formation of rigid compact nanofibers even at physiological pH. The electrostatic interaction of the positively charged Ca2+ ions onto the negatively charged surface carboxylate groups of ToCNF and Fmoc-FF is analyzed by zeta potential (ζ) measurements. Small-angle X-ray scattering measurements give deeper structural insights into the interaction of Fmoc-FF with ToCNF. Cell responses to the hydrogels are studied in human dermal fibroblasts (NHDFs) in a direct contact test using a live/dead assay and in extract test using Alamar Blue and lactate dehydrogenase assays. The results show that high loading of Fmoc-FF decreases cell viability, while additional cross-linking with calcium reduces this cytotoxic effect.

• Remartinez, Enrique Oreja; Zaytseva-Zotova, Daria; Rogala, Agnes; Bautista, Alejandro Barrantes; Messersmith, Phillip B. & Tiainen, Hanna (2025). Multifunctional tannic acid and polyamino acid layer-by-layer coatings for tailored implant surfaces. ACS Biomaterials Science and Engineering. 11(9), s. 5376–5388. doi: 10.1021/acsbiomaterials.5c00832. Fulltekst i vitenarkiv
• Rogala, Agnes; Tiainen, Hanna & Zaytseva-Zotova, Daria (2024). Using QCM-D for Real-Time Analysis of Cell Adhesion Dynamics at Biointerfaces. Advanced Materials Interfaces. doi: 10.1002/admi.202400811. Fulltekst i vitenarkiv Vis sammendrag

  Efficient cell adhesion is crucial for host tissue attachment and improving implant integration. This study aims at investigating adhesion of human gingival fibroblasts to functionalized biomaterial surfaces using quartz crystal microbalance with dissipation (QCM-D) and analyzing the dynamics of focal adhesion (FA) formation during this process. The QCM-D responses are correlated with changes in cell morphology and activation of focal adhesion kinase (FAK) in the absence or presence of serum proteins. As model surfaces, this work uses polystyrene oxidized by UV/ozone treatment (PSox) and tannic acid nanocoating formed on polystyrene (TA60). Compared to PSox, reduced cell adhesion on TA60 with fibroblast establishing fewer FAs is observed in this work. The addition of serum promotes more stable cell adhesion, although the adsorbed protein corona slows initial cell spreading and FAK activation. Further, this work examines whether there is a relationship between QCM-D responses and FAK activation. The data shows a positive correlation between the energy dissipation and temporal changes in phosphorylated FAK (pY397FAK), although the absolute value of pY397FAK cannot be derived from the QCM-D response. Thus, this study demonstrates the usefulness of the QCM-D as a technique for quantitative non-invasive analysis of cell adhesion to functionalized surfaces.

• Rogala, Agnes; Zaytseva-Zotova, Daria; Remartinez, Enrique Oreja; Bautista, Alejandro Barrantes & Tiainen, Hanna (2024). Combining QCM-D with live-cell imaging reveals the impact of serum proteins on the dynamics of fibroblast adhesion on tannic acid-functionalised surfaces. Biomaterials Science. ISSN 2047-4830. 12, s. 3345–3359. doi: 10.1039/d4bm00184b. Fulltekst i vitenarkiv Vis sammendrag

  Nanocoatings based on plant polyphenols have been recently suggested as a potent strategy for modification of implant surfaces for enhancing host cell attachment and reducing bacterial colonisation. In this study we aimed to investigate how serum proteins impact the early adhesion dynamics of human gingival fibroblasts onto titanium surfaces coated with tannic acid (TA). Silicate-TA nanocoatings were formed on titanium and pre-conditioned in medium supplemented with 0, 0.1, 1 or 10% FBS for 1 hour. Dynamics of fibroblasts adhesion was studied using quartz crystal microbalance with dissipation (QCM-D). Time-lapse imaging was employed to assess cell area and motility, while immunofluorescence microscopy was used to examine cell morphology and focal adhesion formation. Our results showed that in serum-free medium, fibroblasts demonstrated enhanced and faster adhesion to TA coatings compared to uncoated titanium. Increasing the serum concentration reduced cell adhesion to nanocoatings, resulting in nearly complete inhibition at 10% FBS. This inhibition was not observed for uncoated titanium at 10% FBS, although cell adhesion was delayed and progressed slower compared to serum-free conditions. In addition, 1% FBS dramatically reduced cell adhesion on uncoated titanium. We revealed a positive relationship between changes in dissipation and changes in cell spreading area, and a negative relationship between dissipation and cell motility. In conclusion, our study demonstrated that serum decreases fibroblasts interaction with surfaces coated with TA in a concentration dependent manner. This suggests that controlling serum concentration can be used to regulate or potentially prevent fibroblasts adhesion onto TA-coated titanium surfaces.

• Kjesbu, Joachim Sebastian; Zaytseva-Zotova, Daria; Sämfors, Sanna; Gatenholm, Paul; Troedsson, Christofer & Thompson, Eric Malcolm [Vis alle 7 forfattere av denne artikkelen] (2022). Alginate and tunicate nanocellulose composite microbeads – Preparation, characterization and cell encapsulation. Carbohydrate Polymers. ISSN 0144-8617. 286. doi: 10.1016/j.carbpol.2022.119284. Fulltekst i vitenarkiv Vis sammendrag

  Alginate has been used for decades for cell encapsulation. Cellulose nanofibrils (CNF) from tunicates are desirable in biomedicine due to high molecular weight, purity, crystallinity, and sustainable production. We prepared microbeads of 400–600 μm of alginate and tunicate CNF. Greater size, dispersity and aspect ratio were observed in microbeads with higher fractions of CNF. CNF content in Ca-crosslinked alginate microbeads decreased stability upon saline exposure, whereas crosslinking with calcium (50 mM) and barium (1 mM) yielded stable microbeads. The Young's moduli of gel cylinders decreased when exchanging alginate with CNF, and slightly increased permeability to dextran was observed in microbeads containing CNF. Encapsulation of MC3T3 cells revealed high cell viability after encapsulation (83.6 ± 0.4%) in beads of alginate and CNF. NHDFs showed lower viability but optimizing mixing and production techniques of microbeads increased cell viability (from 66.2 ± 5.3% to 72.7 ± 7.5%).

• Weber, Florian; Figueira, Louise Morais Dornelas; Hafiane, Nora; Zaytseva-Zotova, Daria; Bautista, Alejandro Barrantes & Petersen, Fernanda Cristina [Vis alle 7 forfattere av denne artikkelen] (2022). Can polyphenolic surface modifications prevent fungal colonization of titanium dental implants? Colloids and Surfaces B: Biointerfaces. ISSN 0927-7765. 219. doi: 10.1016/j.colsurfb.2022.112813. Fulltekst i vitenarkiv Vis sammendrag

  Oral biofilms can be a major health problem causing infections and chronic inflammation of mucosal tissue. While much effort is put in the investigation of bacteria in biofilms, the role of fungi is often neglected, despite Candida albicans playing a key role in the formation of multispecies oral biofilms. With the rise of antibiotic resistance, new strategies to reduce microbial growth need to be found. Therefore, plant derived polyphenolic molecules have been suggested to reduce both adhesion and growth of pathogenic bacteria and fungi. In this study, we investigated the use of polyphenolic coatings to reduce adhesion and biofilm formation of C. albicans BWP17 on titanium implants. Tannic acid and pyrogallol coatings altered the hydrophobic and charge properties of titanium surfaces, and both compounds were gradually released as active molecules over time. Despite such effects, we found no significant inhibition on growth and biofilm formation of C. Albicans, indicating that the release of active molecules from the coatings did not reach relevant inhibitory concentrations. However, a potential antibiofilm effect was observed by the pH-dependent disassembly of the polyphenolic layer, which caused the biofilm to detach. Hence, further efforts are required to create tailored implant surfaces, which sustainably reduce microbial growth and adhesion.

• Omtvedt, Line Aanerud; Kristiansen, Kåre Andre; Strand, Wenche Iren; Aachmann, Finn Lillelund; Strand, Berit Løkensgard & Zaytseva-Zotova, Daria (2021). Alginate hydrogels functionalized with β-cyclodextrin as a local paclitaxel delivery system. Journal of Biomedical Materials Research. Part A. ISSN 1549-3296. 109(12), s. 2625–2639. doi: 10.1002/jbm.a.37255. Fulltekst i vitenarkiv Vis sammendrag

  Modification of drug delivery materials with beta-cyclodextrins (β-CyD) is known to increase solubility of poorly water-soluble drugs, protect drugs from degradation and sustain release. In this study, we developed a hydrogel drug delivery system for local paclitaxel delivery using the natural polysaccharide alginate functionalized with β-CyD-moieties. Paclitaxel was chosen due to its ability to form inclusion complexes with cyclodextrins. The rheological and mechanical properties of the prepared hydro- gels were characterized, as well as in vitro release of the paclitaxel and in vitro activ- ity on PC-3 prostate cancer cells. Introduction of β-CyD-moieties into the hydrogel reduces the mechanical properties of the gels compared to nonmodified gels. How- ever, gelation kinetics were not markedly different. Furthermore, the β-CyD-modified alginate helped to reduce undesired crystallization of the paclitaxel in the gel and facilitated paclitaxel diffusion out of the gel network. Remarkably, the β-CyD grafted alginate showed increased capacity to complex paclitaxel compared to free HPβ- CyD. Release of both paclitaxel and degradation products were measured from the gels and were shown to have cytotoxic effects on the PC-3 cells. The results indicate that functionalized alginate with β-CyDs has potential as a material for drug delivery systems.

• Akbarzadeh, Anita; Koernig, Andre; Kjesbu, Joachim Sebastian; Zaytseva-Zotova, Daria; Sletmoen, Marit & Strand, Berit Løkensgard (2021). High resolution imaging of soft alginate hydrogels by atomic force microscopy. Carbohydrate Polymers. ISSN 0144-8617. 276. doi: 10.1016/j.carbpol.2021.118804. Fulltekst i vitenarkiv
• Victoria, Moreno-Manzano; Zaytseva-Zotova, Daria; López-Mocholí, Eric; Briz-Redón, Álvaro; Strand, Berit Løkensgard & Serrano-Aroca, Angel (2020). Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro. International Journal of Molecular Sciences. ISSN 1661-6596. 21(8608), s. 1–14. doi: 10.3390/ijms21228608. Fulltekst i vitenarkiv Vis sammendrag

  Biologic scaffolds composed of extracellular matrix components have been proposed to repair and reconstruct a variety of tissues in clinical and pre-clinical studies. Injectable gels can fill and conform any three-dimensional shape and can be delivered to sites of interest by minimally invasive techniques. In this study, a biological gel was produced from a decellularized porcine urinary bladder by enzymatic digestion with pepsin. The enzymatic digestion was confirmed by visual inspection after dissolution in phosphate-buffered saline solution and Fourier-transform infrared spectroscopy. The rheological and biological properties of the gel were characterized and compared to those of the MatrigelTM chosen as a reference material. The storage modulus G’ reached 19.4 ± 3.7 Pa for the 30 mg/mL digested decellularized bladder gels after ca. 3 h at 37 °C. The results show that the gel formed of the porcine urinary bladder favored the spontaneous differentiation of human and rabbit adipose-derived stem cells in vitro into smooth muscle cells to the detriment of cell proliferation. The results support the potential of the developed injectable gel for tissue engineering applications to reconstruct for instance the detrusor muscle part of the human urinary bladder.

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• Remartinez, Enrique Oreja; Zaytseva-Zotova, Daria; Pal, Subhajit; Messersmith, Phillip B. & Tiainen, Hanna (2025). Development of a Novel Coating to Modulate Hypoxic Signaling for Enhanced Implant Tissue Regeneration. Fulltekst i vitenarkiv
• Ernø, Inger Thea Myklebust; Zaytseva-Zotova, Daria; Heyward, Catherine Anne & Tiainen, Hanna (2025). Tannic acid and arginine nanocoatings for soft tissue integration of titanium dental implants. Fulltekst i vitenarkiv
• Remartinez, Enrique Oreja; Tiainen, Hanna; Bautista, Alejandro Barrantes & Zaytseva-Zotova, Daria (2024). Tannic acid-protein interaction in coated titanium surfaces. Fulltekst i vitenarkiv
• Remartinez, Enrique Oreja; Tiainen, Hanna; Zaytseva-Zotova, Daria & Bautista, Alejandro Barrantes (2024). Interaction of tannic acid and proteins in titanium surfaces. Fulltekst i vitenarkiv
• Rogala, Agnes; Zaytseva-Zotova, Daria; Baran, Anna; Petersen, Fernanda Cristina & Tiainen, Hanna (2024). Inactivated probiotics on tannic acid nanocoating - enhancing bioactivity for improved bone and soft tissue integration. Fulltekst i vitenarkiv
• Rogala, Agnes; Tiainen, Hanna & Zaytseva-Zotova, Daria (2024). QCM-D-based analysis of cell adhesion: a relationship between QCM-D signal change and focal adhesion kinase activation. Fulltekst i vitenarkiv
• Rogala, Agnieszka; Zaytseva-Zotova, Daria; Bautista, Alejandro Barrantes & Tiainen, Hanna (2023). QCM-D combined with live cell imaging reveals inverse correlation between cell adhesion to tannic acid nanocoatings and serum concentration in medium. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Hoel, Andrea Draget; Mjelle, Robin & Strand, Wenche [Vis alle 13 forfattere av denne artikkelen] (2023). Alginate hydrogel characteristics regulate NHDF biology. Fulltekst i vitenarkiv
• Strand, Berit Løkensgard; Haslene-Hox, Hanne; Sætrom, Pål; Zaytseva-Zotova, Daria; Mjelle, Robin & Arlov, Øystein [Vis alle 12 forfattere av denne artikkelen] (2023). Alginate hydrogel characteristics regulate NHDF biology. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Singh, Simon; Haideri, Frozan; Elkafas, Nadin Abdelwahab Mohamed Abdou; Bautista, Alejandro Barrantes & Singh, Preet Bano [Vis alle 7 forfattere av denne artikkelen] (2023). Capsaicin-loaded microdroplets for the treatment of burning mouth syndrome. Fulltekst i vitenarkiv
• Remartínez, Enrique Oreja; Tiainen, Hanna; Rogala, Agnieszka; Bautista, Alejandro Barrantes & Zaytseva-Zotova, Daria (2023). Tannic Acid/Polyamino acid Layer-by-Layer coating and their interaction with human cells. Fulltekst i vitenarkiv
• Haslene-Hox, Hanne; Chahal, Aman Singh; Arlov, Øystein; Øynebråten, Ingrid Ryge; Zaytseva-Zotova, Daria & Vikenes, Anette [Vis alle 16 forfattere av denne artikkelen] (2023). Alginate hydrogel characteristics regulate fibroblast biology. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Vikenes, Anette; Hoel, Andrea Draget & Chahal, Aman S [Vis alle 15 forfattere av denne artikkelen] (2021). Alginate Hydrogel Characteristics Regulate Dermal Fibroblasts Biology. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria (2021). Successful use of 3DLife methodology across species. [Internet]. https://www.digitallifenorway.org/research/3dlife/news/succe. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Haslene-Hox, Hanne; Arlov, Øystein; Vikenes, Anette; Hoel, Andrea Draget & Chahal, Aman S [Vis alle 13 forfattere av denne artikkelen] (2020). Varying single parameters of the alginate hydrogels to design better microenvironments for fibroblasts cultivation in vitro. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Ruohola, Rita; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne & Arlov, Øystein [Vis alle 12 forfattere av denne artikkelen] (2019). Soft alginate hydrogels modified with bioactive peptides as extracellular matrices for 3D fibroblast culture. Fulltekst i vitenarkiv
• Kjesbu, Joachim Sebastian; Zaytseva-Zotova, Daria; Solheim, Ingeborg Elisabeth & Strand, Berit Løkensgard (2019). Alginate microspheres with polycationic coatings for in vitro cell cultivation​. Fulltekst i vitenarkiv
• Hoel, Andrea Draget; Haslene-Hox, Hanne; Arlov, Øystein; Klinkenberg, Geir; Vikenes, Anette & Åslund, Andreas [Vis alle 11 forfattere av denne artikkelen] (2019). 3DLife: Validating new high-throughput methods for 3D cell culture screening. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Ruohola, Rita; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne & Arlov, Øystein [Vis alle 14 forfattere av denne artikkelen] (2019). Alginate hydrogels with tailored mechanical and biological properties for 3D fibroblast culture. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Strand, Wenche Iren; Akbarzadeh, Anita; Rouhola, Rita; Haslene-Hox, Hanne & Arlov, Øystein [Vis alle 11 forfattere av denne artikkelen] (2019). Optimizing alginate gelation conditions for 3D cell culture. Fulltekst i vitenarkiv
• Zaytseva-Zotova, Daria; Strand, Wenche Iren; Akbarzadeh, Anita; Haslene-Hox, Hanne; Arlov, Øystein & Vikenes, Anette [Vis alle 10 forfattere av denne artikkelen] (2018). Alginate-based biomimetic matrices for 3D cell culture and high throughput screening. Fulltekst i vitenarkiv

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