Enrique Oreja Remartinez

• Remartinez, Enrique Oreja; Zaytseva-Zotova, Daria; Rogala, Agnes; Bautista, Alejandro Barrantes; Messersmith, Phillip B. & Tiainen, Hanna (2025). Multifunctional tannic acid and polyamino acid layer-by-layer coatings for tailored implant surfaces. ACS Biomaterials Science and Engineering. 11(9), s. 5376–5388. doi: 10.1021/acsbiomaterials.5c00832. Fulltekst i vitenarkiv
• Rogala, Agnes; Zaytseva-Zotova, Daria; Remartinez, Enrique Oreja; Bautista, Alejandro Barrantes & Tiainen, Hanna (2024). Combining QCM-D with live-cell imaging reveals the impact of serum proteins on the dynamics of fibroblast adhesion on tannic acid-functionalised surfaces. Biomaterials Science. ISSN 2047-4830. 12, s. 3345–3359. doi: 10.1039/d4bm00184b. Fulltekst i vitenarkiv Vis sammendrag

  Nanocoatings based on plant polyphenols have been recently suggested as a potent strategy for modification of implant surfaces for enhancing host cell attachment and reducing bacterial colonisation. In this study we aimed to investigate how serum proteins impact the early adhesion dynamics of human gingival fibroblasts onto titanium surfaces coated with tannic acid (TA). Silicate-TA nanocoatings were formed on titanium and pre-conditioned in medium supplemented with 0, 0.1, 1 or 10% FBS for 1 hour. Dynamics of fibroblasts adhesion was studied using quartz crystal microbalance with dissipation (QCM-D). Time-lapse imaging was employed to assess cell area and motility, while immunofluorescence microscopy was used to examine cell morphology and focal adhesion formation. Our results showed that in serum-free medium, fibroblasts demonstrated enhanced and faster adhesion to TA coatings compared to uncoated titanium. Increasing the serum concentration reduced cell adhesion to nanocoatings, resulting in nearly complete inhibition at 10% FBS. This inhibition was not observed for uncoated titanium at 10% FBS, although cell adhesion was delayed and progressed slower compared to serum-free conditions. In addition, 1% FBS dramatically reduced cell adhesion on uncoated titanium. We revealed a positive relationship between changes in dissipation and changes in cell spreading area, and a negative relationship between dissipation and cell motility. In conclusion, our study demonstrated that serum decreases fibroblasts interaction with surfaces coated with TA in a concentration dependent manner. This suggests that controlling serum concentration can be used to regulate or potentially prevent fibroblasts adhesion onto TA-coated titanium surfaces.

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• Remartinez, Enrique Oreja; Zaytseva-Zotova, Daria; Pal, Subhajit; Messersmith, Phillip B. & Tiainen, Hanna (2025). Development of a Novel Coating to Modulate Hypoxic Signaling for Enhanced Implant Tissue Regeneration. Fulltekst i vitenarkiv
• Remartinez, Enrique Oreja; Tiainen, Hanna; Bautista, Alejandro Barrantes & Zaytseva-Zotova, Daria (2024). Tannic acid-protein interaction in coated titanium surfaces. Fulltekst i vitenarkiv
• Remartinez, Enrique Oreja; Tiainen, Hanna; Zaytseva-Zotova, Daria & Bautista, Alejandro Barrantes (2024). Interaction of tannic acid and proteins in titanium surfaces. Fulltekst i vitenarkiv
• Remartínez, Enrique Oreja; Tiainen, Hanna; Rogala, Agnieszka; Bautista, Alejandro Barrantes & Zaytseva-Zotova, Daria (2023). Tannic Acid/Polyamino acid Layer-by-Layer coating and their interaction with human cells. Fulltekst i vitenarkiv

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