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Liebert Parreiras Nogueira

Sjefingeniør - Klinisk forskningslaboratorium

E-post l.p.nogueira@odont.uio.no

Telefon +47 22 85 52 38

Mobiltelefon +47 48 15 98 53

Brukernavn

Besøksadresse Geitmyrsveien 71 0455 Oslo

Postadresse Postboks 1109 Blindern 0317 Oslo

Last ned visittkort

Next - Norwegian Centre for Nano-scale X-ray Tomography

Academic Interests

MicroCT, NanoCT, Synchrotron Radiation, X-ray imaging, Scaffolds, Image analysis, Histomorphometric quantification.

Higher education and employment history

Liebert P. Nogueira is a former Professor from Rio de Janeiro State University (Brazil), where he worked for 6 years, and got involved in many activities, such as teaching and supervising Master and Ph.D

* Ph.D (2011) also in Nuclear Engineer, from Rio de Janeiro Federal University, Brazil

* Master of Science (2008) in Nuclear Engineer, from Rio de Janeiro Federal University, Brazil

* Post-doctoral Research (2016) at the Physics Department of the State University of Londrina, Brazil.

Reppe, Sjur; Nogueira, Liebert Parreiras; Yu, Fangtang; Valland, Haldor; Gautvik, Vigdis Teig & Utheim, Tor Paaske [Vis alle 9 forfattere av denne artikkelen] (2026). Integrated analysis of human osteocyte lacunar ultrastructure and bone proteome profiling reveals coupling between immune and osteogenic signaling pathways. Bone. ISSN 8756-3282. 213. doi: 10.1016/j.bone.2026.118077.
Saiz, Augustine Mark; Jamous, Reem; Stötzel, Sabine; Rahmati, Maryam; Holmboe, Leonard Volrath & Johnson, Soren [Vis alle 16 forfattere av denne artikkelen] (2026). Limited Added Effect of Proline‐Rich P6 Peptide to Cross‐Linked Hyaluronic Acid After Partial Lateral Meniscectomy in Sheep. Smart Medicine. ISSN 2751-1863. 5(4). doi: 10.1002/smmd.70048. Fulltekst i vitenarkiv Vis sammendrag
This study evaluates whether a proline‐rich P6 peptide provides added benefit to a cross‐linked hyaluronic acid (HA) hydrogel after partial lateral meniscectomy‐associated osteoarthritis‐like change in sheep. The corrected four‐group framework includes Intact no‐surgery/no‐injection reference knees ( n = 7) and 16 surgically injured knees from eight sheep assigned to Mnx (meniscectomy only, n = 4), Mnx + HA ( n = 6), or Mnx + HA + P6 ( n = 6). Mnx + HA and Mnx + HA + P6 knees received intra‐articular injections at Weeks 0, 1, 2, and 4. Osteochondral tissues were evaluated at Week 12 by microCT, H&E scoring, and histochemical/immunohistochemical image analysis. Endpoint‐specific animal‐ or knee‐level models were used, with sheep identity included as a random effect when estimable. HA‐based treatment was associated with favorable trabecular microCT patterns after meniscectomy. However, the primary Mnx + HA + P6 versus Mnx + HA contrast was not significant for percent trabecular bone volume/BV/TV (+1.52 percentage points; 95% CI −2.22 to 5.26; p = 0.427). Histology and IHC/histochemical findings showed selected exploratory tissue‐pattern signals, including collagen‐associated matrix staining, but did not establish consistent peptide‐specific superiority. These findings support cross‐linked HA as an intra‐articular biomaterial platform while defining requirements for confirmatory HA‐peptide hydrogel studies.
Rosenqvist, Marija Plahter; Dunkel, Kristina; Nogueira, Liebert Parreiras; Planke, Sverre; Polteau, Stephane & Ardit, M. [Vis alle 8 forfattere av denne artikkelen] (2026). Natural CO<inf>2</inf>sequestration: mechanisms and conditions of carbonate precipitation in basalt. Geochimica et Cosmochimica Acta. ISSN 0016-7037. 427, s. 381–402. doi: 10.1016/j.gca.2026.07.013. Fulltekst i vitenarkiv
Rubenis, Kristaps; He, Xin; Demir, Öznur; Fu, Zhaoyue; Sigurjónsson, Ólafur Eysteinn & Hildebrand, Torben [Vis alle 13 forfattere av denne artikkelen] (2026). Eggshell-derived amorphous calcium phosphate for upcycled bone repair: An In-Vivo study. Materials Today Communications. 53. doi: 10.1016/j.mtcomm.2026.115391. Fulltekst i vitenarkiv Vis sammendrag
Critical-sized bone defects require grafting materials that support early osteogenesis and predictable defect bridging. In this study, we evaluated eggshell-derived amorphous calcium phosphate (ACP) granules in a rat calvarial defect model and compared them with ACP obtained from commercial hydroxyapatite (HAp) and with cross-linked hyaluronic acid (HA) gel alone. Eggshell-derived and HAp-derived ACP were synthesized by the same dissolution–precipitation route, using formulations previously characterized in detail for composition, amorphous phase stability, and in vitro bioactivity. Bilateral 5 mm calvarial defects in rats were filled with HA gel (INT/HA), HA + HAp-derived ACP (Control ACP), or HA + eggshell-derived ACP (Eggshell ACP). Grafts were mixed immediately before implantation using a dual-syringe system. Bone regeneration at 2 and 4 weeks was assessed by micro-computed tomography and histology. Both ACP-containing groups enhanced bone volume fraction and reduced trabecular separation compared with HA alone, particularly in the central region of the defect. Eggshell ACP promoted more consistent defect bridging, with a continuous lamellar bone-like layer and more mature collagenous and mineralized tissue at the bone–graft interface. Within the limitations of a non-load-bearing small-animal model, these findings indicate that eggshell-derived ACP combined with HA supports early bone regeneration and exhibits higher osteogenic activity than HA alone and HAp-derived ACP, warranting further investigation as a bone graft candidate.
Alzoubi, Fawaz M.; Altemimi, Aseel; Nogueira, Liebert Parreiras & Yilmaz, Burak (2026). Microcomputed tomography analysis of marginal adaptation and internal fit of zirconia frameworks with direct-to-abutment connections using a deep learning artificial intelligence model. The Journal of prosthetic dentistry (Print). ISSN 0022-3913. doi: 10.1016/j.prosdent.2026.04.032. Fulltekst i vitenarkiv Vis sammendrag
Statement of problem Despite the increased use of direct-to-abutment connections for implant-supported fixed complete dental prostheses (ISFCDPs), limited evidence exists regarding marginal adaptation and internal fit, 2 parameters critical for biomechanical performance. Purpose This study evaluated the marginal adaptation and internal fit of zirconia frameworks with direct-to-abutment connections compared to conventional titanium base (Ti-base) connections. A novel method for internal fit assessment using a deep learning artificial intelligence (AI) model was also introduced, and level of agreement with traditional measurements was assessed. Internal fit was compared across the 3 designs using both traditional and AI-derived measurements. Material and methods Zirconia specimens (n=24) were allocated to 3 groups: frameworks with Ti-base and regular screws (n=8), frameworks fabricated direct-to-abutment with regular screws (n=8), and frameworks fabricated direct-to-abutment with modified screws (n=8). Each specimen underwent microcomputed tomography scanning before and after thermomechanical aging (TMA) to assess internal fit and marginal adaptation. A deep learning-based AI segmentation model was developed using an image processing software program and achieved a final validation loss below 0.01, corresponding to a Dice Similarity Coefficient greater than 0.95. This metric indicates high spatial overlap and robust segmentation of the gap and abutment regions. Volumetric measurements were obtained for 1 straight and 1 angled transmucosal abutment per specimen. Specimens were subjected to 1.2 million loading cycles at 90 N, followed by repeated measurements. Ninety-six sites were evaluated before and after TMA. Two measurements each for straight and angled abutments for the 3 designs were averaged and difference scores were calculated as post-TMA minus pre-TMA values. Nonparametric statistical tests were applied because of heterogeneity of group variances. Post hoc Mann–Whitney U tests, intraclass correlation and Bland-Altman analyses were conducted (α=.05). Results Significant differences in marginal adaptation among the 3 groups were identified before and after TMA. The Ti-base group demonstrated significantly superior marginal adaptation compared to both direct-to-abutment groups at both time points (z=3.36, P<.001). Significant differences in internal fit were also observed among the 3 groups before and after TMA. Ti-base group showed significantly improved internal fit relative to both direct-to-abutment groups (standardized Mann–Whitney z values ranged from 3.15 to 3.36, P<.001). Intraclass correlations revealed a significant level of agreement between standardized measurements obtained using conventional and AI-based methods (P<.001), and Bland-Altman plots provided support for concurrent validity of the AI-derived measures of internal fit. Conclusions Ti-base connections in ISFCDPs demonstrated superior marginal adaptation and internal fit compared to direct-to-abutment connections. Both connection designs achieved clinically acceptable internal fit values. For marginal adaptation, the direct-to-abutment regular screw group exceeded the clinically acceptable threshold of 120 µm. A significant level of agreement was found between standardized traditional and AI-derived measurements of internal fit. Bland-Altman plots supported the concurrent validity of the deep learning AI model.
Procházková, Petra; Parobková, Viktória; Hildebrand, Torben; Nogueira, Liebert Parreiras; Vrlíková, Lucie & Buchtová, Marcela [Vis alle 8 forfattere av denne artikkelen] (2026). Contrast Agents and Sample Preparation Strategies for Soft Tissue Imaging in X-ray Computed Microtomography: Review and Experimental Validation in Mouse Tissues. Tomography of Materials and Structures (JTMS). 11. doi: 10.1016/j.tmater.2026.100086. Fulltekst i vitenarkiv Vis sammendrag
Micro-computed tomography (micro-CT) is an effective technique for non-destructive high-resolution imaging of mineralized tissues; however, imaging of soft tissues is challenging due to their inherently low X-ray attenuation and poor contrast. Therefore, their visualization in absorption micro-CT requires the use of contrast agents (CA) such as iodine-based solutions, osmium tetroxide, phosphotungstic acid, and other polyoxometalates. The aim of this review is to outline the individual steps of sample preparation before micro-CT measurement, such as fixation, dehydration, and contrast enhancement of soft tissues, and summarize the most commonly used CAs in ex vivo visualization. In addition to the literature overview, we conducted comparative staining experiments on mouse organs using several widely applied CAs, with the aim of illustrating practical outcomes and challenges. Based on both the data published and our test results, we recommend the most suitable CAs for different tissue types, with emphasis on minimizing tissue damage and deformation, and devote particular attention to applications in mouse embryos.
Altemimi, Aseel; Alzoubi, Fawaz; Nogueira, Liebert Parreiras & Yilmaz, Burak (2026). Screw preload analysis of direct-to-abutment connections in complete arch zirconia frameworks before and after thermomechanical aging. The Journal of prosthetic dentistry (Print). ISSN 0022-3913. doi: 10.1016/j.prosdent.2026.03.018. Fulltekst i vitenarkiv Vis sammendrag
Statement of problem Direct-to-abutment connections have been increasingly used for implant-supported fixed complete dentures (ISFCDs). However, the biomechanical performance of these connections and their screws under simulated clinical conditions has not been adequately investigated. Purpose The purpose of this in vitro study was to investigate the reverse torque values (RTVs) of ISFCDs connected to transmucosal abutments with and without the use of a Ti-base interface before and after thermomechanical aging (TMA). In addition, the study aimed to compare RTV according to the implant position and location (straight anterior and angled posterior) within each group. Material and methods Zirconia frameworks (N=24) for restorations supported by 4 implants were divided into 3 groups: frameworks with Ti bases and regular screws, direct-to-abutment frameworks with regular screws, and direct-to-abutment frameworks with modified screws. All frameworks were 3-dimensionally designed and subtractively manufactured. The RTVs of screws and preload efficiency (PE) were measured after tightening the screws. The frameworks were subjected to 1.2 million cycles at 90 N, 2 Hz with a dwell time of 30 seconds between 5 °C and 55 °C in distilled water with a total of 12 000 thermal cycles. RTV and PE measurements were repeated after TMA. Paired comparisons were performed using the Wilcoxon signed-rank test, and post hoc Mann-Whitney U tests with Bonferroni adjustment were used to evaluate RTV and PE loss after TMA. In addition, the Mann-Whitney U tests were used to compare the differences in preload loss between straight anterior and angled posterior abutments in each design. The Cohen r was used to estimate effect sizes for all group comparisons (α=.05). Results Before TMA, the median RTV was 13.3 Ncm, and the median PE was 88.3%. After TMA, the median RTV was 11.5, and the median PE was 76.7%, with significant loss of preload across the entire sample (z=6.06, P<.001). However, the Mann-Whitney U tests revealed that the losses were not significantly different between the standard Ti-base connection and either the direct-to-abutment connection with regular screws (P=.518) or the direct-to-abutment connection with modified screws (P=.664). Furthermore, no significant differences were observed between abutment positions in the Ti-Base connection with regular screws (P=.880) or between abutment positions in the direct-to-abutment connection with modified screws (P=.363). However, comparison of direct-to-abutment connections revealed significantly greater losses in angled posterior abutments with regular screws (z=−2.25, P=.025). Conclusions No significant differences in RTV and PE were observed between Ti-base and direct-to-abutment connections before and after TMA. Modified screw designs did not significantly affect preload before TMA in direct-to-abutment connections. However, they significantly minimized preload loss after TMA in angled posterior abutments only in the direct-to-abutment connection compared with that with regular screws in the Ti-base and the direct-to-abutment connections using regular screws.
Ma, Qianli; Staudigel, Katrin Anja; Rubenis, Kristaps; Fu, Zhaoyue; Hildebrand, Torben & Zaldenieks, Markuss [Vis alle 12 forfattere av denne artikkelen] (2026). Sustainable Eggshell-Based Amorphous Calcium Phosphate Scaffolds and Membrane Protein Hydrogel for Regeneration in Rabbit Femoral and Calvarial Defects. ACS Applied Materials & Interfaces. ISSN 1944-8244. doi: 10.1021/acsami.5c25453. Fulltekst i vitenarkiv Vis sammendrag
Critical-sized bone defects require grafts with ideal spatial supporting. We upcycled eggshell waste sustainably into porous amorphous calcium phosphate (ACP) scaffolds and evaluated their early bone-regeneration performance alone and in combination with flexible and bioactive adjuncts in vivo. ACP scaffolds with ∼60% porosity were fabricated by pressure-densification, using ice microspheres as space holders. Before implantation, ACP scaffolds were vacuum-infiltrated with a hyaluronic acid (HA) hydrogel to minimize internal voids. Bilateral critical-size femoral and calvarial defects were created in New Zealand White rabbits and reconstructed with one of four treatments: (1) Sham, (2) ACP scaffold (ACP), (3) ACP infused with HA hydrogel containing a proline-rich peptide P2 (ACP + HA/P2), or (4) ACP infused with HA/P2 supplemented with soluble eggshell membrane proteins (prepared by our lab, ACP + HA/P2 + SEPs). After 6 weeks, micro-CT and histology were performed. All ACP-containing groups showed greater defect filling and mineralization than the Sham group. Micro-CT analysis demonstrated that ACP + HA/P2 + SEPs achieved the highest bone volume and trabecular number and the lowest trabecular separation. Histology confirmed more extensive mineralized collagenous deposition on ACP scaffolds and a more integrated bone–material fusion zone in this group. In femoral defects with graft displacement, ACP scaffolds induced cortical–scaffold bone bridging within the marrow, most evident with SEPs. Eggshell-derived ACP scaffolds, particularly when paired with SEP-enriched HA hydrogel, enhance early regeneration of critical-sized bone defects. Upcycling the inorganic and organic constituents of eggshell waste yields a complementary, single-origin biomaterials package for sustainable bone repair.
Riordan, Claire; Cappellesso, Vanessa Giaretton; Kumar, Padmapriya Arul; Nogueira, Liebert Parreiras; Simonsen, Siri & Gruyaert, Elke [Vis alle 10 forfattere av denne artikkelen] (2026). Development of polyurethane-shelled microcapsules using membrane emulsification for self-sealing cementitious applications. Cement & Concrete Composites. ISSN 0958-9465. 167, s. 106440–106440. doi: 10.1016/j.cemconcomp.2025.106440. Fulltekst i vitenarkiv
Berdiell, Izar Capel; Cordero-Lanzac, Tomás; Junge, Nicolai Haaber; Nogueira, Liebert Parreiras; Vaughan, Gavin & Michiel, Marco Di [Vis alle 10 forfattere av denne artikkelen] (2025). X-ray absorption and diffraction computed tomography characterization of deactivation and coking in spray-dried ZSM-5/alumina catalysts. Catalysis Science & Technology. ISSN 2044-4753. doi: 10.1039/d5cy01023c. Fulltekst i vitenarkiv Vis sammendrag
One of the main drawbacks of acid-based heterogeneous catalytic processes involving hydrocarbons is coke formation. Still, research on shaped catalysts remains limited. The main objective of this study was to gain insight into the catalyst deactivation in the methanol-to-hydrocarbon (MTH) reaction. Diffraction and absorption computed tomography experiments were performed on spray dried, hollow semi-spherically shaped ZSM-5/alumina catalysts of approximately 250 microns in size. The catalysts were employed in the MTH reaction at two different pressures, resulting in varying degrees of coking. Absorption tomography (0.027 μm3 per voxel) revealed the structural features and sponginess of the shaped catalysts. These are not perfect spheres; they rather have openings as they burst during the spray drying process. Further, high resolution powder X-ray diffraction computed tomography slices (0.125 μm3 per voxel) were analyzed by parametric Rietveld refinement. The analysis showed that the catalyst and binder overall are rather homogeneously spatially distributed within each sphere, but bubbles and agglomerates of a single phase are frequent. In addition, it is demonstrated that there were no coking gradients across the sphere wall at both partial and full deactivation. This indicates that the binder and the catalyst shape and size were suitable for the reaction conditions. Indeed, the catalyst lifetime was almost doubled relative to the pure, powdered zeolite catalyst. A series of catalysts with varying degrees of deactivation have been fully characterized ex situ, suggesting significant spillover of coke from the zeolite to the alumina matrix. These findings demonstrate the need for greater efforts to understand the formulation of shaped catalyst objects, where the matrix should not only hold the components together but also support and enhance the overall catalytic process.
Santos, Mariana de S; Araujo-Lopes, Roberta; Alves, Isabele B P; Campideli-Santana, Ana C; Henriques, Patricia C & Santos, Diogo M M dos [Vis alle 11 forfattere av denne artikkelen] (2025). SAT-807 Kisspeptin Prevents Bone Loss Caused by the Lack of Estrogen in Ovariectomized Rodents. Journal of the Endocrine Society (JES). 9(Supplement_1). doi: 10.1210/jendso/bvaf149.658. Fulltekst i vitenarkiv Vis sammendrag
Abstract Disclosure: M.D. Santos: None. R. Araujo-Lopes: None. I.B. Alves: None. A.C. Campideli-Santana: None. P.C. Henriques: None. D.M. dos Santos: None. L.P. Nogueira: None. G.J. Dias: None. D.R. Grattan: None. S. Macari: None. R.E. Szawka: None. Kisspeptin (Kp) may exert direct action on bone. We tested the effect of Kp10 in preventing or reversing bone loss caused by ovariectomy in female rats and mice and determined the direct action of Kp10 on osteoblasts (OBL) and osteoclasts (OCL). Female Wistar rats and C57BL/6 mice were ovariectomized (OVX) or sham-operated. To characterize the temporal profile of bone loss, Sham and OVX rats were investigated at 1, 2, and 6 months (m) after ovariectomy. To evaluate the effects of Kp10, OVX and Sham rats were treated with s.c. Kp10 (1 nmol/day), saline (Veh), or E2. OVX mice received Kp10 at 0.5 or 1 nmol/day. The treatment duration was 30 days, starting on day 1 (prevention protocol, in rats and mice) or 30 (reversal protocol, in rats) after ovariectomy. Hormonal analyses were performed by ELISA, and the femur was analyzed by micro-CT, histology, and qPCR. Primary cultures of mouse OBL and OCL received 10-8, 10-7, or 10-6 M Kp10. In trabecular bone, ovariectomy decreased bone mineral density (BMD), bone volume ratio (BV/TV), and trabecular number (Tb.N), and increased trabecular separation (Tb.Sp) in all time points evaluated. Sham rats had reduced BMD and Tb.N at 6m. Ovariectomy gradually increased the quantity of bone marrow cells and adipocytes. In the cortical bone, ovariectomy increased the periosteal and endocortical perimeters and decreased the cortical/total area fraction after 1m and 2m. OVX rats exhibited increased body weight and luteinizing hormone levels and decreased uterine weight, effects restored by E2 but not Kp10. Ovariectomy also reduced E2 and testosterone and increased RANKL plasma levels. Kp10 treatment starting 1 day after ovariectomy was as effective as E2 in preventing the reductions in BV, BV/TV, and Tb.N and the increase in Tb.Sp, whereas only E2 prevented the decrease in BMD. When treatment started 30 days after ovariectomy, neither Kp10 nor E2 recovered bone loss. In OVX mice, 1 nmol Kp10 prevented the rise in plasma RANKL/OPG ratio and the reduction in BV, BV/TV, Tb.N, and Tb.Th. Kp10 also prevented the increases in Tnf-α and Il-6 and the decrease in Alp expression in the femur of OVX mice. In cell culture, all concentrations of Kp10 increased the deposition of OBL mineral nodules but did not affect OCL differentiation. In conclusion, Kp10 prevents bone loss caused by E2 withdrawal, enhancing OBL activity and mineralized matrix deposition while suppressing bone pro-inflammatory interleukins associated with osteoclastogenesis. Presentation: Saturday, July 12, 2025
Saiz, Augustine Mark; Rahmati, Maryam; Johnson, Soren; Bhat, Aneesh Satish; Baldini, Tony Daniel & Øvrebø, Øystein [Vis alle 13 forfattere av denne artikkelen] (2025). Systemic versus local delivery of mesenchymal stem cells to improve the early stages of fracture healing in a polytrauma model. Journal of Biological Engineering. 19(1). doi: 10.1186/s13036-025-00554-4. Fulltekst i vitenarkiv Vis sammendrag
Fracture healing complications, including nonunion, are common in polytrauma patients partly due to systemic inflammatory dysregulation. Although mesenchymal stem cells (MSCs) have been widely explored for their regenerative properties, their therapeutic potential in polytrauma patients remains uncertain. Given the clinical interest in both systemic and localized stem cell therapies, understanding how delivery route influences MSCs biodistribution, inflammatory modulation, and therapeutic efficacy is critical for optimizing treatment strategies in polytrauma. Hence, we compared systemic versus local MSCs delivery in a polytrauma model. We evaluated inflammatory responses and bone formation in a C57BL/6J murine model across four groups: (1) isolated fracture, (2) polytrauma (femur fracture + chest trauma), (3) polytrauma + systemic MSC delivery, and (4) polytrauma + local MSC delivery in hyaluronic acid-based hydrogels at the fracture site. Polytrauma induced a prolonged inflammatory response characterized by elevated interleukin 1 alpha and beta (IL-1α & β), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and monocyte chemoattractant protein-1 and − 5 (MCP-1 & MCP-5). Both delivery methods significantly reduced inflammation and proinflammatory cytokines, though local delivery yielded more consistent effects. IVIS imaging confirmed MSC retention at the fracture site in the local delivery group, while systemic administration of MSCs resulted in pulmonary entrapment. Although systemic MSCs failed to enhance fracture healing significantly, local MSC delivery promoted bone formation evidenced by CT and histological characterizations. These findings demonstrate that local MSC delivery in a hydrogel scaffold represents a superior strategy for improving fracture healing in polytrauma patients compared to systemic delivery.
Pizzetti, Fabio; Vanoli, Valeria; Magaletti, Roberta; Nogueira, Liebert Parreiras; Gelosa, Simone & Haugen, Håvard Jostein [Vis alle 10 forfattere av denne artikkelen] (2025). Multiple release of fertilizers and pesticides from biodegradable bicontinuous structures. Journal of Industrial and Engineering Chemistry. ISSN 1226-086X. doi: 10.1016/j.jiec.2025.02.053. Fulltekst i vitenarkiv Vis sammendrag
Bicontinuous bijel like structures have been of relevant importance in biomedical field in recent years thanks to their biocompatibility and ability to successfully load and release both hydrophilic and hydrophobic drugs. Their structure can also be tuned by changing the nanoparticles that stabilize the system constituted by organic and the aqueous phases in similar amount. These qualities can also promote their usage in many other fields. Agrochemical industry, for instance, is currently searching for some multiple fertilizer/pesticide release systems in order to increase the fertilizer efficiency when treating the soil on one side, since much of them are lost in the environment as contaminants. On the other the use of pesticides is, year by year, limited by very strict rules and so their smart delivery is pivotal to achieve agriculture goals without causing pollution. In this work, the single loading and release of a representative fertilizer (urea) and a representative pesticide (atrazine) through a bijel-based structure has been studied. In particular, their effect on the structure properties have been considered, as far as the release properties in a different media, simulating what is happening in crop production.
Giorgi, Zoe; Veneruso, Valeria; Petillo, Emilia; Vangosa, Francesco Briatico; Nogueira, Liebert Parreiras & Haugen, Håvard Jostein [Vis alle 9 forfattere av denne artikkelen] (2025). Exploring the Role of Aqueous Buffered Saline Solutions on the Macroscopic Properties of PEG/Carbomer/Agarose Hydrogels. Macromolecular Bioscience. ISSN 1616-5187. s. 2500073–2500073. doi: 10.1002/mabi.202500073. Fulltekst i vitenarkiv Vis sammendrag
Nowadays, during hydrogel formulation design, the focus is primarily on polymers and cross-linkers. It is crucial to ensure a high level of biocompatibility and degradability, along with appropriate physico-chemical properties. However, other ingredients can also influence hydrogel formation and performance. Therefore, this study examines the effect of different salt molarities in phosphate buffered saline (PBS) solutions, used as reaction solvents, on the properties of hydrogels mainly composed of polyethylene glycol, agarose, and carbomer. To conduct the study, two PBS solutions are prepared by varying the salt concentrations, and these mixtures are used as solvents in a bulk polycondensation reaction. Subsequently, the two hydrogel formulations obtained are characterized to analyze the effect of PBS concentration on the material properties. An increase in salt concentration reduced the swelling capacity and release ability of the hydrogel matrix, as well as decreased the porosity and interconnectivity of the material, contributing to non-homogeneity in the pore network. Consequently, these results highlight that the salinity and ionic strength of buffering solutions can affect various material properties, and therefore, careful consideration should be given during the preparation of these solutions.
Capotorto, Elena; Chiudaroli, Licia; Montrasio, Edoardo; Nogueira, Liebert Parreiras; Pitton, Matteo & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2025). Toward improved auricle reconstruction: The role of FDM 3D printing with PCL and TPU materials. Biomaterials Advances. ISSN 2772-9516. 169. doi: 10.1016/j.bioadv.2025.214175. Fulltekst i vitenarkiv Vis sammendrag
Microtia, along with trauma, represents one of the main causes of external ear malformation. Different clinical techniques were developed for the reconstruction of the auricle, but they all have some drawbacks. This work is focused on the development of an innovative 3D porous scaffold, printed by Fused Deposition Modelling (FDM) and based on laser-scanned images of the healthy contralateral ear of the patient. The scaffold was printed using polycaprolactone (PCL) and thermoplastic polyether urethane (TPU) to mimic the components of the cartilage and adipose tissue. After the optimization of the printing parameters, the 3D surface obtained as an output of the laser scan was elaborated, sliced and used as input for printing the layered structure. Morphological analysis confirmed the shape fidelity compared to the CAD model, and good pore interconnection was observed through microCT investigation. Mechanical compression and torsion tests were performed to verify that the mechanical properties of the 3D PCL/TPU structure were adequate. The 3D structure exhibited elastic moduluses comparable to those of the cartilaginous and adipose parts of the auricle. The torsion tests showed the adequacy of the structure without detachment between TPU and PCL printed layers. In vitro cell viability tests confirmed the absence of cytotoxicity in both materials. The PCL/TPU layered scaffold reproduced the anatomy of the patient's healthy contralateral ear, representing a good compromise between flexibility and strength. This, along with the other assessed properties, make this scaffold a valid alternative for the reconstruction of the external ear.
Santos, Lúcia F.; Mendes, Maria C; Pereira, Joao F; Dias, Isabel R; Viegas, Carlos A & Amorim, Carlos O [Vis alle 11 forfattere av denne artikkelen] (2025). Remote-Controlled Magnetic Stimulation of Cell-Based Bioengineered Tissues for In Situ Bone Regeneration. Advanced Materials. ISSN 0935-9648. doi: 10.1002/adma.202500657. Fulltekst i vitenarkiv Vis sammendrag
The native cell microenvironment activates signaling pathways through mechanotransduction mechanisms, influencing cells’ physiological and functional outcomes. Magnetic fields are explored to manipulate these environments, and magnetic nanoparticles (MNPs) are highlighted as nano-instructive agents capable of activating key signaling pathways, presenting exciting possibilities in tissue engineering. Still, the ability to precisely control the assembly and differentiation of stem cells within a dynamically responsive microenvironment, crucial for effective tissue regeneration, remains unexplored. This study showcases a novel method wherein MNPs facilitate the precise assembly of magnetically responsive cells into complex 3D tissue structures upon internalization and exposure to temporally defined cyclic magnetic fields. By remotely stimulating these constructs, it is demonstrated for the first time the possibility of remote-controlled modulation of stem cell fate in vivo without biochemical supplementation. Notably, this approach led to ectopic bone formation, highlighting the ability of magnetic actuation to drive osteogenesis in non-bone environments. MNP-driven mechanical stimulation of implanted tissues functions as a bioresponsive system guiding osteogenic differentiation of human adipose-derived stem cells. The in vivo model further illustrates accelerated construct integration, enhanced osteogenic differentiation, and minimal local inflammation, underscoring the potential of this less invasive, remotely controllable platform to advance regenerative strategies for bone
Palombo, David; Rivara, Federico; Nogueira, Liebert Parreiras; Vignoletti, Fabio; Esporrin, Javier Sanz & Haugen, Håvard Jostein [Vis alle 7 forfattere av denne artikkelen] (2025). Three-Dimensional Hard And Soft Tissue Healing At Implants With A Modified Neck Design: An In Vivo Preclinical Study. Clinical Implant Dentistry and Related Research. ISSN 1523-0899. doi: 10.1111/cid.70065. Fulltekst i vitenarkiv
Hildebrand, Torben; Haugen, Håvard Jostein; Romandini, Mario; Plotino, Gianluca & Nogueira, Liebert Parreiras (2025). From Pulp to Cementum: 3D Visualization of Soft and Hard Dental Tissues Using Different Micro-CT Contrast-Enhancement Techniques. International Endodontic Journal. ISSN 0143-2885. doi: 10.1111/iej.14260. Fulltekst i vitenarkiv Vis sammendrag
Aim: To determine the effect of two contrast-enhancement strategies in nano-computed tomography (nano-CT) imaging on the contrast-to-noise ratio (CNR) of various dental tissues, including pulp, dentine and cementum, with the goal of enhancing the visibility of dental soft tissues to a level not yet reported in laboratory nano-CT imaging. Methodology: Ten sound human third molars underwent decalcification and subsequent treatment with Lugol’s iodine (n=5, Group 1) or phosphotungstic acid (PTA) treatment without prior decalcification (n=5, Group 2) for contrast enhancement. Imaging was performed using the laboratory nano-CT system Skyscan 2211 and the synchrotron radiation for medical physics (SYRMEP) beamline. CNRs were measured for pulpal tissue, dentine and cementum and nano-CT images were compared with classical histology, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDXS). Results: Group 1 significantly enhanced the contrast of pulp tissue, resulting in a 168.2 % increase due to decalcification and an additional 148.7 % increase after Lugol’s iodine treatment. Dentine exhibited higher contrast in Group 2, whereas cementum showed similar contrast across both groups. Laboratory nano-CT enabled the visualisation of detailed soft tissue structures, including nerves, blood vessels and odontoblasts within the pulp, but cementocytes remained not visibleinvisible. Conclusions: Decalcification followed by Lugol’s iodine treatment was superior for enhancing soft tissue contrast, especially for pulp visualisation. PTA without decalcification yielded better contrast for dentine and facilitated the visualisation of attached soft tissues, such as periodontal ligament and predentine. These findings provide insights into selecting the most appropriate protocol to optimise nano-CT imaging for specific dental tissue analyses, including the pulp.
Sena, Gabriela; Alvarenga, Thaina; Feio, Ruan Ingliton; Mello, Cicero Brasileiro; Fidalgo, Gabriel & Paiva, Katrine [Vis alle 16 forfattere av denne artikkelen] (2025). Virtual dissection of Aedes aegypti mosquito using phase-contrast synchrotron microtomography. Journal of Microscopy. ISSN 0022-2720. doi: 10.1111/jmi.70004. Fulltekst i vitenarkiv
Øvrebø, Øystein; Orlando, Luca; Rubenis, Kristaps; Ciriello, Luca; Ma, Qianli & Giorgi, Zoe [Vis alle 13 forfattere av denne artikkelen] (2024). The role of collagen and crystallinity in the physicochemical properties of naturally derived bone grafts. Regenerative Biomaterials. 11. doi: 10.1093/rb/rbae093. Fulltekst i vitenarkiv Vis sammendrag
Xenografts are commonly used for bone regeneration in dental and orthopaedic domains to repair bone voids and other defects. The first-generation xenografts were made through sintering, which deproteinises them and alters their crystallinity, while later xenografts are produced using cold-temperature chemical treatments to maintain the structural collagen phase. However, the impact of collagen and the crystalline phase on physicochemical properties have not been elucidated. We hypothesized that understanding these factors could explain why the latter provides improved bone regeneration clinically. In this study, we compared two types of xenografts, one prepared through a low-temperature chemical process (Treated) and another subsequently sintered at 1100 °C (Sintered) using advanced microscopy, spectroscopy, x-ray analysis, and compressive testing. Our investigation showed that the Treated bone graft was free of residual blood, lipids, or cell debris, mitigating the risk of pathogen transmission. Meanwhile, the sintering process removed collagen and the carbonate phase of the Sintered graft, leaving only calcium phosphate and increased mineral crystallinity. Micro computed tomography revealed that the Treated graft exhibited an increased high porosity (81%) and pore size compared to untreated bone, whereas the Sintered graft exhibited shrinkage, which reduced the porosity (72%), pore size, and strut size. Additionally, scanning electron microscopy (SEM) displayed crack formation around the pores of the Sintered graft. The Treated graft displayed median mechanical properties comparable to native cancellous bone and clinically available solutions, with an apparent modulus of 166 MPa, yield stress of 5.5 MPa, and yield strain of 4.9%. In contrast, the Sintered graft exhibited a lower median apparent modulus of 57 MPa. It failed in a brittle manner at a median stress of 1.7 MPa and strain level of 2.9%, demonstrating the structural importance of the collagen phase. This indicates why bone grafts prepared through cold-temperature processes are clinically favourable.
Nooraiepour, Mohammad; Polański, Krzysztof; Masoudi, Mohammad; Kuczyński, Szymon; Derluyn, Hannelore & Nogueira, Liebert Parreiras [Vis alle 9 forfattere av denne artikkelen] (2024). Potential for 50% Mechanical Strength Decline in Sandstone Reservoirs Due to Salt Precipitation and CO2–Brine Interactions During Carbon Sequestration. Rock Mechanics and Rock Engineering. ISSN 0723-2632. 58, s. 1239–1269. doi: 10.1007/s00603-024-04223-8. Fulltekst i vitenarkiv Vis sammendrag
Predictive modeling of CO2 storage sites requires a detailed understanding of physico-chemical processes and scale-up challenges. Dramatic injectivity decline may occur due to salt precipitation pore clogging in high-salinity aquifers during subsurface CO2 injection. This study aims to elucidate the impact of CO2-induced salt crystallization in the porous medium on the geomechanical properties of reservoir sandstones. As the impact of salt precipitation cannot be isolated from the precursor interactions with CO2 and acidified brine, we present a comprehensive review and discuss CO2 chemo-mechanical interactions with sandstones. Laboratory geochemical CO2–brine–rock interactions at elevated pressures and temperatures were conducted on two sandstone sets with contrasting petrophysical qualities. Interaction paths comprised treatment with (a) CO2-acidified brine and (b) supercritical injection until brine dry-out, salt crystallization, and growth. Afterward, the core samples were tested in a triaxial apparatus at varying stresses and temperatures. The elastic moduli of intact, CO2-acidified brine treated, and salt-affected sandstones were juxtaposed to elucidate the geochemical–geomechanical-coupled impacts and identify the extent of crystallization damages. The salt-affected sandstones showed a maximum of 50% reduction in Young’s and shear moduli and twice an increase in Poisson’s ratio compared to intact condition. The deterioration was notably higher for the tighter reservoir sandstones, with higher initial stiffness and lower porosity–permeability. We propose two pore- and grain-scale mechanisms to explain how salt crystallization contributes to stress localization and mechanical damage. The results highlight the potential integrity risk imposed by salt crystallization in (hyper)saline aquifers besides injectivity, signaling mechanical failure exacerbated by pressure buildup.
Monteiro, Ana Carolina; Garcia, Diego de Andrade; Rocher, Barbara Du; Fontão, Ana Paula; Nogueira, Liebert Parreiras & Fidalgo, Gabriel [Vis alle 8 forfattere av denne artikkelen] (2024). Cooperation between T and B cells reinforce the establishment of bone metastases in a mouse model of breast cancer. Bone. ISSN 8756-3282. 178. doi: 10.1016/j.bone.2023.116932. Fulltekst i vitenarkiv
Hildebrand, Torben; Ma, Qianli; Loča, Dagnija; Rubenis, Kristaps; Locs, Janis & Nogueira, Liebert Parreiras [Vis alle 7 forfattere av denne artikkelen] (2024). Improved visualisation of ACP-engineered osteoblastic spheroids: a comparative study of contrast-enhanced micro-CT and traditional imaging techniques. Biofabrication. ISSN 1758-5082. 17(1). doi: 10.1088/1758-5090/ad8bf5. Fulltekst i vitenarkiv Vis sammendrag
This study investigates osteoblastic cell spheroid cultivation methods, exploring flat-bottom, U-bottom, and rotary flask techniques with and without amorphous calcium phosphate (ACP) supplementation to replicate the 3D bone tissue microenvironment. ACP particles derived from eggshell waste exhibit enhanced osteogenic activity in 3D models. However, representative imaging of intricate 3D tissue-engineered constructs poses challenges in conventional imaging techniques due to notable scattering and absorption effects in light microscopy, and hence limited penetration depth. We investigated contrast-enhanced micro-CT as a methodological approach for comprehensive morphological 3D-analysis of the in-vitro model and compared the technique with confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and classical histology. Phosphotungstic acid (PTA) and iodine-based contrast agents were employed for micro-CT imaging in laboratory and synchrotron micro-CT imaging. Results revealed spheroid shape variations and structural integrity influenced by cultivation methods and ACP particles. The study underscores the advantage of 3D spheroid models over traditional 2D cultures in mimicking bone tissue architecture and cellular interactions, emphasising the growing demand for novel imaging techniques to visualise 3D tissue-engineered models. Contrast-enhanced micro-CT emerges as a promising non-invasive imaging method for tissue-engineered constructs containing ACP particles, offering insights into sample morphology, enabling virtual histology before further analysis.
Randers, Engelke Marie; Kibsgård, Thomas Johan; Nogueira, Liebert Parreiras; Kjensjord, Trygve; Röhrl, Stephan Maximilian & Nordsletten, Lars [Vis alle 7 forfattere av denne artikkelen] (2024). Osseointegration of minimally invasive sacroiliac joint fixation implants—A human retrieval study. Journal of Orthopaedic Research. ISSN 0736-0266. 42, s. 1820–1830. doi: 10.1002/jor.25820. Fulltekst i vitenarkiv Vis sammendrag
Minimally invasive sacroiliac joint fusion has become increasingly prevalent and is described to reduce pain and improve function. In some patients, pain can recur several months after primary surgery. Lack of early implant osseointegration might be a cause of pain and hence an indication for revision surgery. Triangular titanium implants are the most documented implant for minimally invasive sacroiliac joint fusion. There is, however, no knowledge of how triangular titanium implants osseointegrate in humans and whether fusion is induced over the sacroiliac joint. During planned revision surgery due to recurrent pain, six triangular titanium implants were retrieved from six different patients at median 9 months from primary surgery. All six implants were scanned using microcomputed tomography. The presence or absence of bone in-growth, on-growth, and through-growth of the implants was evaluated as an indication of implant osseointegration. Three of six implants showed no or minor signs of osseointegration. Of the three remaining implants, one showed partial osseointegration and two implants showed high degrees of osseointegration. This study showed that triangular titanium implants can osseointegrate into host bone in humans. When osseointegration occurs, triangular titanium implants can give fusion across the sacroiliac joint.
Reppe, Sjur; Reseland, Janne Elin; Prijatelj,, Vid; Prediger, Michael; Nogueira, Liebert Parreiras & Utheim, Tor Paaske [Vis alle 9 forfattere av denne artikkelen] (2024). The DLEU2–miR-15a–16-1 Cluster Is a Determinant of Bone Microarchitecture and Strength in Postmenopausal Women and Mice. International Journal of Molecular Sciences. ISSN 1661-6596. 25(23), s. 12724–12724. doi: 10.3390/ijms252312724. Fulltekst i vitenarkiv Vis sammendrag
This study explores how select microRNAs (miRNAs) influence bone structure in humans and in transgenic mice. In trabecular bone biopsies from 84 postmenopausal women (healthy, osteopenic, and osteoporotic), we demonstrate that DLEU2 (deleted in lymphocytic leukemia 2)-encoded miR-15a-5p is strongly positively associated with bone mineral density (BMD) at different skeletal sites. In bone transcriptome analyses, miR-15a-5p levels correlated positively with the osteocyte characteristic transcripts SOST (encoding sclerostin) and MEPE (Matrix Extracellular Phosphoglycoprotein), while the related miR-15b-5p showed a negative association with BMD and osteoblast markers. The data imply that these miRNAs have opposite roles in bone remodeling with distinct actions on bone cells. Expression quantitative trait loci (eQTL) variants confirmed earlier DLEU2 associations. Furthermore, a novel variant (rs12585295) showed high localization with transcriptionally active chromatin states in osteoblast primary cell cultures. The supposition that DLEU2-encoded miRNAs have an important regulatory role in bone remodeling was further confirmed in a transgenic mice model showing that miR-15a/16-1-deleted mice had significantly higher percentage bone volume and trabecular number than the wild type, possibly due to prenatal actions. However, the three-point mechanical break force test of mice femurs showed a positive correlation between strength and miR-15a-5p/miR-16-5p levels, indicating differential effects on cortical and trabecular bone. Moreover, these miRNAs appear to have distinct and complex actions in mice prenatally and in adult humans, impacting BMD and microstructure by regulating bone cell transcription. However, detailed interactions between these miRNAs and their downstream mechanisms in health and disease need further clarification.
Nooraiepour, Mohammad; Polanski, Krzysztof; Masoudi, Mohammad; Kuczyński, Szymon; Derluyn, Hannelore & Nogueira, Liebert Parreiras [Vis alle 9 forfattere av denne artikkelen] (2024). Assessing salt precipitation and weak acid interaction in subsurface CO2 injection: Potential 50% strength decline in near-wellbore reservoir sandstones. arXiv. doi: 10.48550/arXiv.2401.14852. Fulltekst i vitenarkiv Vis sammendrag
Predictive modeling of CO2 storage sites requires a detailed understanding of physico-chemical processes and potential challenges for scale-up. Dramatic injectivity decline may occur due to salt precipitation pore clogging in high-salinity reservoirs, even over a short time frame. This study aims to elucidate the adverse impact of CO2-induced salt crystallization in porous media on the geomechanical properties of near-wellbore reservoir sandstones. As the impact of salt precipitation cannot be isolated from the precursor effects of interaction with CO2 and carbonic acid, we initiated our study by a comprehensive review of CO2 chemo-mechanical interactions with sandstones. We conducted laboratory geochemical CO2-brine-rock interactions at elevated pressures and temperatures on two sets of porous sandstone with contrasting petrophysical qualities. Two paths were followed: treatment with (a) CO2-acidified brine at 10 MPa fluid pressure and 60C for 7 days, and a second subset continuation with (b) supercritical injection until complete dry-out and salt precipitation. Afterward, the core samples were tested in a triaxial apparatus at varying stresses and temperatures. The elastic moduli of intact, CO2-reacted, and salt-damaged sandstones were juxtaposed to elucidate the extent of crystallization damages. The salt-affected specimens showed a maximum of 50 percent reduction in Young's and shear moduli and twice an increase in Poisson's ratio compared to intact condition. The deterioration was notably higher for the tighter rocks with higher initial stiffness.
Haugen, Håvard Jostein; Ma, Qianli; Linskens, Stefanie; Par, Matej; Mandic, Visnja Negovetic & Mensikova, Emile [Vis alle 13 forfattere av denne artikkelen] (2024). 3D micro-CT and O-PTIR spectroscopy bring new understanding of the influence of filler content in dental resin composites. Dental Materials. ISSN 0109-5641. doi: 10.1016/j.dental.2024.09.001. Fulltekst i vitenarkiv Vis sammendrag
Background Dental resin composites' performance is intricately linked to their polymerisation shrinkage characteristics. This study compares polymerisation shrinkage using advanced 3D micro-computed tomography (micro-CT) and traditional 2D linear assessments. It delves into the crucial role of filler content on shrinkage and the degree of conversion in dental resin composites, providing valuable insights for the field. Methods Five experimental dental composite materials were prepared with increasing filler contents (55–75 wt%) and analysed using either 3D micro-CT for volumetric shrinkage or a custom-designed linometer for 2D linear shrinkage. The degree of conversion was assessed using Optical Photothermal Infrared (O-PTIR) and Fourier-Transform Infrared (FTIR) spectroscopy. Light transmittance through a 2-mm layer was evaluated using a NIST-calibrated spectrometer. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDX) examined surface morphology and elemental distribution. Correlation between the investigated parameters was determined using Spearman correlation analyses. Results The study found significant differences in polymerisation-related properties among different filler content categories, with volumetric shrinkage consistently demonstrating higher mean values than linear shrinkage across most groups. Volumetric shrinkage decreased with increasing curing depth, showing no direct correlation between filler content and shrinkage levels at different curing depths. The results highlighted a strong negative correlation between filler content and degree of conversion, volumetric and linear shrinkage, as well as maximum shrinkage rate. Light transmittance showed a moderate correlation with the filler content and a weak correlation with other tested parameters. Conclusions This study underscores the importance of considering both volumetric and linear shrinkage in the design and analysis of dental composite materials. The findings advocate optimising filler content to minimise shrinkage and enhance material performance. Integrating micro-CT and O-PTIR techniques offers novel insights into dental composites' polymerisation behaviour, providing a foundation for future research to develop materials with improved clinical outcomes.
Marovic, Danijela; Haugen, Håvard Jostein; Par, Matej; Linskens, Stefanie; Mensikova, Emile & Mandic, Visnja Negovetic [Vis alle 10 forfattere av denne artikkelen] (2024). Emerging technologies for the evaluation of spatio-temporal polymerisation changes in flowable vs. sculptable dental resin-based composites. Dental Materials. ISSN 0109-5641. doi: 10.1016/j.dental.2024.09.002. Fulltekst i vitenarkiv Vis sammendrag
Background: This study presents a novel multi-technique approach that integrates micro-CT and optical photothermal infrared spectroscopy (O-PTIR) to evaluate polymerisation differences, so-called spatio-temporal polymerisation properties, between flowable and sculptable dental resin-based composites. Methods: Ten commercially available dental composites were investigated, including flowable and sculptable counterparts from the same manufacturer. Eight parameters were evaluated: short-term polymerisation characteristics (degree of conversion after 5 min, maximum polymerisation rate, time to reach maximum polymerisation rate) was measured using ATR-FTIR with real-time monitoring; changes in the degree of conversion with depth were evaluated with O-PTIR, 3D visualisation of shrinkage patterns, overall volumetric shrinkage, depth-specific shrinkage, and porosity were measured using micro-CT; surface morphology with detailed measurements of elemental composition was characterised using SEM/EDX; light transmittance was analysed with a NIST-referenced spectrometer. Results: The study found that the increase in filler weight and volume ratio reduced the degree of conversion and polymerisation shrinkage, while moderately influencing the maximum polymerisation rates. The time to reach maximum polymerisation rates and light transmittance were not dependent on the filler amount. OPTIR assessed a depth-dependent decrease in the degree of conversion for both composite types, with flowable composites generally showing a greater decrease in the degree of conversion than sculptable composites, except for bulk-fill composites. Micro-CT scans showed significantly higher flowable shrinkage values than their sculptable counterparts, highlighting the performance differences between the two types of composites. Conclusions: The findings of this study have practical implications for the selection and use of dental composites. Flowable composites, despite their higher degrees of conversion and polymerisation rates, also exhibit higher volumetric shrinkage, which can be detrimental for clinical applications. The new measurement methods used in this study provide a comprehensive overview of the polymerisation behaviour of commercially available dental composites, offering valuable insights for material optimisation.
Hildebrand, Torben; Ma, Qianli; Heyward, Catherine Anne; Haugen, Håvard Jostein & Nogueira, Liebert Parreiras (2024). Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT. Journal of Medical Imaging. ISSN 2329-4302. 11(6). doi: 10.1117/1.JMI.11.6.066001. Fulltekst i vitenarkiv Vis sammendrag
Purpose: Micro-CT analysis of soft tissues alongside bone remains challenging due to significant differences in X-ray absorption, preventing spatial inspection of bone remodeling including the cellular intricacies of mineralized tissues in developmental biology and pathology. The goal was to develop a protocol for contrast-enhanced micro-CT imaging that effectively visualizes soft tissues and cells in conjunction with bone while minimizing bone attenuation by decalcification. Approach: Murine femur samples were decalcified in EDTA and treated with three different contrast agents: i) iodine in ethanol, ii) PTA in water, and iii) Lugol’s iodine. Micro-CT scans were performed in the laboratory set-up SkyScan 1172 and at the SYRMEP beamline in ELETTRA. Soft- and hard-tissue contrast-to-noise ratio (CNR) and contrast efficiency after decalcification were measured. Results: In laboratory micro-CT, Lugol’s iodine demonstrated a three-times higher CNR in bone marrow, representing the soft tissue portion, compared to bone. Contrast efficiencies, measured in synchrotron micro-CT, were consistent with these findings. Higher resolutions and the specificity of Lugol’s iodine to cellular structures enabled detailed visualization of bone-forming cells in the epiphyseal plate. Conclusions: The combination of decalcification and the utilization of the contrast agent Lugol’s iodine facilitated an enhanced soft tissue visualization in conjunction with bone.
Hildebrand, Torben; Humphris, Yolanda; Haugen, Håvard Jostein & Nogueira, Liebert Parreiras (2024). Contrast-enhanced Micro-CT Imaging of Murine Mandibles: A Multimethod Approach for Simultaneous Hard and Soft Tissue Analysis. Journal of Clinical Periodontology. ISSN 0303-6979. s. 1–10. doi: 10.1111/jcpe.14077. Fulltekst i vitenarkiv Vis sammendrag
Aim To develop and evaluate a novel multi-method micro-computed tomography (μCT) imaging protocol for enhanced visualization of both hard and soft tissues in murine mandibles, addressing the limitations of traditional imaging techniques in dental research. Materials and Methods We employed a contrast-enhanced (CE) μCT imaging technique using Lugol's iodine as a contrast agent to visualize the intricate structures of murine mandibles. The protocol involved the combination of conventional μCT imaging as well as CE-μCT, including decalcification with EDTA, allowing for simultaneous assessment of hard and soft tissues. The method is compared with standard imaging modalities, and the ability to visualize detailed anatomical features is discussed. Results The CE-μCT imaging technique provided superior visualization of murine mandibular structures, including dental pulp, periodontal ligaments and the surrounding soft tissues, along with conventional μCT imaging of alveolar bone and teeth. This method revealed detailed anatomical features with high specificity and contrast, surpassing traditional imaging approaches. Conclusion Our findings demonstrate the potential of CE-μCT imaging with Lugol's iodine as a powerful tool for dental research. This technique offers a comprehensive view of the murine mandible, facilitating advanced studies in tissue engineering, dental pathology and the development of dental materials.
Alonso, Iván; Haugen, Håvard Jostein; Nogueira, Liebert Parreiras; López-Álvarez, Miriam; González, Pío & López-Peña, Mónica [Vis alle 8 forfattere av denne artikkelen] (2024). Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures. Polymers. 16(9). doi: 10.3390/polym16091243. Fulltekst i vitenarkiv Vis sammendrag
This study investigates the effect of scaffold architecture on bone regeneration, focusing on 3D-printed polylactic acid-bioceramic calcium phosphate (PLA-bioCaP) composite scaffolds in rabbit femoral condyle critical defects. We explored two distinct scaffold designs to assess their influence on bone healing and scaffold performance. Structures with alternate (0° /90°) and helical (0°/45°/90°/135°/180°) laydown patterns were manufactured with a 3D printer using a fused deposition modeling technique. The scaffolds were meticulously characterized for pore size, strut thickness, porosity, pore accessibility, and mechanical properties. The in vivo efficacy of these scaffolds was evaluated using a femoral condyle critical defect model in eight skeletally mature New Zealand White rabbits. Then, the results were analyzed micro-tomographically, histologically, and histomorphometrically. Our findings indicate that both scaffold architectures are biocompatible and support bone formation. The helical scaffolds, characterized by larger pore sizes and higher porosity, demonstrated significantly greater bone regeneration than the alternate structures. However, their lower mechanical strength presented limitations for use in load-bearing sites.
Daood, Umer; Al-Nabulsi, Mohammad; Qasim, Saad; Sidhu, Preena; Saxena, Kirti & Banavar, Spoorthi Ravi [Vis alle 11 forfattere av denne artikkelen] (2024). Novel GSK-3 inhibitor based universal dentin bonding system: A comprehensive analysis of dentinal repair and improved bond strength. International Journal of Adhesion and Adhesives. ISSN 0143-7496. 135. doi: 10.1016/j.ijadhadh.2024.103839. Fulltekst i vitenarkiv Vis sammendrag
The aim of the study was to describe the formulation of a novel universal adhesive system, based on a GSK-3 (glycogen synthetase kinase-3) inhibitor (Tideglusib®), and to evaluate its bond strength and dentinal repair capability. Tideglusib® was added to Ultradent Peak Universal Dentin Bonding Agent to make experimental adhesives (0.5 %, 1 %, 2 % final concentration). They were applied to teeth which were sectioned, stored for 24 h, or 2 years then bond strengths measured. Baseline stiffness of bonded dentin beams was tested using the three-point bending test. Animal experiments were performed to provide specimens for microcomputed tomography imaging after adhesive application. Collagen matrix was evaluated using transmission electron microscopy. The cytotoxicity of the modified adhesives was determined using fibroblastic cells. Molecular modelling evaluated binding of the Tideglusib® molecule to active sites on collagen and the adhesive components. TGFβ-1/and BMP-2 in human dentin matrices were measured and interfaces were scanned using SkyScan 2211. Adhesives’ stability tests were done. Results RMSD (root mean square deviation) analysis indicated simulation has equilibrated with changes for globular proteins (p < 0.05). Specimens from 0.5 %, and 1 % Tideglusib® groups displayed adhesive penetration with Micro-CT imaging and a significant increase in dentin density was seen. Specimens from the same groups showed cells with large masses of cytoplasm with focal adhesion points. Bond strength decreased significantly (p < 0.05) with increased storage time. Gradual release (p < 0.05) of both TGFβ-1 and BMP-2 from hTDMs with 0.5 % and 1 % Tideglusib® groups occurred and higher elastic moduli (p < 0.05). Collagen-based amide and proline Raman bands were seen in the Tideglusib® groups. Conclusion Incorporation of Tideglusib® in experimental Universal Adhesive System at 1 % improved adhesive bond strength and promoted dentinal repair. Application of Tideglusib® based adhesive conserve mechanical properties of the tooth structure and promote regenerative capability so can be expected to extend the service life of adhesive restorations in clinical situations.
Lemonche, Gerard Boix; Hildebrand, Torben; Haugen, Håvard Jostein; Petrovski, Goran & Nogueira, Liebert Parreiras (2024). Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea. Heliyon. 10(3). doi: 10.1016/j.heliyon.2024.e25828. Fulltekst i vitenarkiv Vis sammendrag
Background The cornea, a vital component of the human eye, plays a crucial role in maintaining visual clarity. Understanding its ultrastructural organization and cell distribution is fundamental for elucidating corneal physiology and pathology. This study comprehensively examines the microarchitecture of the hydrated human cornea using contrast-enhanced micro-computed tomography (micro-CT). Method Fresh human corneal specimens were carefully prepared and hydrated to mimic their in vivo state. Contrast enhancement with Lugol's iodine-enabled high-resolution Micro-CT imaging. The cells' three-dimensional (3D) distribution within the cornea was reconstructed and analyzed. Results The micro-CT imaging revealed exquisite details of the corneal ultrastructure, including the spatial arrangement of cells throughout its depth. This novel approach allowed for the visualization of cells' density and distribution in different corneal layers. Notably, our findings highlighted variations in cell distribution between non-hydrated and hydrated corneas. Conclusions This study demonstrates the potential of contrast-enhanced micro-CT as a valuable tool for non-destructive, 3D visualization and quantitative analysis of cell distribution in hydrated human corneas. These insights contribute to a better understanding of corneal physiology and may have implications for research in corneal diseases and tissue engineering.
Fernández, Iván Alonso; Haugen, Håvard Jostein; Nogueira, Liebert Parreiras; Álvarez, Miriam López; González, Pío & Peña, Mónica López [Vis alle 8 forfattere av denne artikkelen] (2024). Bone regeneration in rabbit cranial defects: 3D printed polylactic acid scaffolds gradually enriched with marine bioderived calcium phosphate. Materialia. 38. doi: 10.1016/j.mtla.2024.102240. Fulltekst i vitenarkiv Vis sammendrag
Objective This study aimed to evaluate the in vivo biocompatibility, mechanical performance and osteoconductive potential of 3D-printed polylactic acid (PLA) scaffolds enriched with marine bioderived calcium phosphate (bioCaP) for bone tissue engineering. Materials and methods PLA-bioCaP composite scaffolds were specifically designed for the rabbit cranial defect model by 3D printing, with a uniform distribution of open square-shaped pores and contributions in bioCaP. Physicochemical and mechanical characterization and the evaluation of biological response are presented. Results The scaffolds demonstrated mechanical properties comparable to human bones, integration with the host bone, and osteoconductive behavior promoting cell ingrowth from the defect edge. Strong mineralized tissue ingrowth through the scaffolds’ pores was observed, providing notable support to the host bone. In quantitative terms, micro-CT and histomorphometry analysis post-implantation revealed no significant differences in bone regeneration across all groups. Conclusion The 3D-printed scaffolds with perpendicular patterning, open porosity, and proposed composition displayed satisfactory mechanical properties, biocompatibility, and osteoconductive response. The scaffolds promoted bone regeneration at similar levels as the PLA. The highest contribution of bioCaP promoted a positive influence in certain histomorphometric parameters; however, it did not significantly improve their osteogenic capability. Further research is required to optimize scaffold composition and enhance their osteogenic potential. Clinical relevance This study presents a significant advancement in bone tissue engineering through the development of personalized composite scaffolds for bone-related applications. The clinical implications of this research are profound, especially considering the increasing demand for functional bone regeneration technologies capable of producing cost-effective producing cost-effective customized scaffolds.
Schot, Maik; Becker, Malin; Paggi, Carlo Alberto; Gomes, Francisca; Koch, Timo & Gensheimer, Tarek [Vis alle 12 forfattere av denne artikkelen] (2023). Photoannealing of Microtissues Creates High-Density Capillary Network Containing Living Matter in a Volumetric-Independent Manner. Advanced Materials. ISSN 0935-9648. doi: 10.1002/adma.202308949. Fulltekst i vitenarkiv Vis sammendrag
The vascular tree is crucial for the survival and function of large living tissues. Despite breakthroughs in 3D bioprinting to endow engineered tissues with large blood vessels, there is currently no approach to engineer high-density capillary networks into living tissues in a scalable manner. We here present photo-annealing of living microtissues(PALM) as a scalable strategy to engineer capillary-rich tissues. Specifically, in-air microfluidics was used to produce living microtissues composed of cell-laden microgels in ultra-high throughput, which could be photo-annealed into a monolithic living matter. Annealed microtissues inherently give rise to an open and interconnected pore network within the resulting living matter. Interestingly, utilizing soft microgels enables microgel deformation, which leads to the uniform formation of capillary-sized pores. Importantly, the ultra-high throughput nature underlying the microtissue formation uniquely facilitates scalable production of living tissues of clinically relevant sizes (>1 cm3 ) with an integrated high-density capillary network. In short, PALM generates monolithic, microporous, modular tissues that meet the previously unsolved need for large engineered tissues containing high-density vascular networks, which is anticipated to advance the fields of engineered organs, regenerative medicine, and drug screening
Moore, Axel C; Hennessy, Matthew G; Nogueira, Liebert Parreiras; Franks, Susan J; Taffetani, Matteo & Seong, Hyejeong [Vis alle 17 forfattere av denne artikkelen] (2023). Fiber Reinforced Hydrated Networks Recapitulate the Poroelastic Mechanics of Articular Cartilage. Acta Biomaterialia. ISSN 1742-7061. doi: 10.1016/j.actbio.2023.06.015. Fulltekst i vitenarkiv Vis sammendrag
The role of poroelasticity on the functional performance of articular cartilage has been established in the scientific literature since the 1960s. Despite the extensive knowledge on this topic there remain few attempts to design for poroelasticity and to our knowledge no demonstration of an engineered poroelastic material that approaches the physiological performance. In this paper, we report on the development of an engineered material that begins to approach physiological poroelasticity. We quantify poroelasticity using the fluid load fraction, apply mixture theory to model the material system, and determine cytocompatibility using primary human mesenchymal stem cells. The design approach is based on a fiber reinforced hydrated network and uses routine fabrication methods (electrohydrodynamic deposition) and materials (poly[ɛ-caprolactone] and gelatin) to develop the engineered poroelastic material. This composite material achieved a mean peak fluid load fraction of 68%, displayed consistency with mixture theory, and demonstrated cytocompatibility. This work creates a foundation for designing poroelastic cartilage implants and developing scaffold systems to study chondrocyte mechanobiology and tissue engineering.
Ning, Ke; Fang, Yang; Bronkhorst, Ewald M.; Ruben, Jan; Nogueira, Liebert Parreiras & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2023). Fatigue behaviour of a self-healing dental composite. Dental Materials. ISSN 0109-5641. 39(10), s. 913–921. doi: 10.1016/j.dental.2023.08.172. Fulltekst i vitenarkiv Vis sammendrag
Objective Novel self-healing resin-based composites containing microcapsules have been developed to improve the mechanical performance of dental restorations. However, the long-term fatigue behaviour of these self-healing composites has still been hardly investigated. Therefore, this manuscript studied the fatigue behaviour of self-healing composites containing microcapsules by subjecting the specimens to traditional staircase tests and ageing in a custom-designed chewing simulator (Rub&Roll) to simulate oral ageing physiologically relevant conditions. Methods To prepare self-healing composite, poly(urea-formaldehyde) microcapsules containing acrylic self-healing liquids were synthesized. Subsequently, these microcapsules (10 wt%) and initiator (benzoyl peroxide, BPO, 2 wt%) were incorporated into a commercial flowable resin-based composite. Microcapsule-free resin-based composites with and without BPO were also prepared as control specimens. A three-point flexural test was used to measure the initial flexural strength (Sinitial). Subsequently, half of the specimens were used for fatigue testing using a common staircase approach to measure the fatigue strengths (FS). In addition, the other specimens were aged in the Rub&Roll machine for four weeks where after the final flexural strength (Sfinal) was measured. Results Compared to Sinitial, FS of all tested specimens significantly decreased as measured through staircase testing. After 4 weeks of ageing in the Rub&Roll machine, Sfinal was significantly reduced compared to Sinitial for microcapsule-free resin-based composites, but not for the self-healing composites (p = 0.3658). However, the self-healing composites are still in the experimental phase characterized by a low mechanical strength, which still impedes further clinical translation. Significance Self-healing composites containing microcapsules exhibit improved fatigue resistance compared to microcapsule-free non-self-healing composites.
Hildebrand, Torben; Novak, Jan; Nogueira, Liebert Parreiras; Boccaccini, Aldo Roberto & Haugen, Håvard Jostein (2023). Durability assessment of hydrogel mountings for contrast-enhanced micro-CT. Micron. ISSN 0968-4328. 174. doi: 10.1016/j.micron.2023.103533. Fulltekst i vitenarkiv Vis sammendrag
Micro-computed tomography (micro-CT) provides valuable data for studying soft tissue, though it is often affected by sample movement during scans and low contrast in X-ray absorption. This can result in lower image quality and geometric inaccuracies, collectively known as 'artefacts'. To mitigate these issues, samples can be embedded in hydrogels and enriched with heavy metals for contrast enhancement. However, the long-term durability of these enhancements remains largely unexplored. In this study, we examine the effects of two contrast enhancement agents – iodine and phosphotungstic acid (PTA) – and two hydrogels – agarose and Poloxamer 407 – over a 14-day period. We used Drosophila melanogaster as a test model for our investigation. Our findings reveal that PTA and agarose are highly durable, while iodine and poloxamer hydrogel exhibits higher leakage rates. These observations lay the foundation for estimating contrast stabilities in contrast-enhanced micro-CT with hydrogel embedding and serve to inform future research in this field.
Sevgi, Usame; Johnsen, Gaute Floer; Hussain, Badra; Piasecki, Lucila; Nogueira, Liebert Parreiras & Haugen, Håvard Jostein (2023). Morphometric micro-CT study of contralateral mandibular incisors. Clinical Oral Investigations. ISSN 1432-6981. 28(1). doi: 10.1007/s00784-023-05419-y. Fulltekst i vitenarkiv Vis sammendrag
Objectives: This study aimed to determine the degree of similarity and symmetry in the anatomy of contralateral mandibular incisors. Three-dimensional (3D) models of extracted teeth were obtained from microtomography (micro-CT) scans. Qualitative and quantitative assessments of the morphology and comparison of contralateral pairs were made. The null hypothesis was that contralateral mandibular incisors could not be considered identical in simple morphometric measurements. Methods: Sixty pairs of mandibular incisors were extracted from 30 patients and scanned with micro-CT. Virtual models of the cemento-enamel junction to the root apex were rendered. Parameters such as length, canal width, dentinal thicknesses, tortuosity, centerline length, accessory canals, root canal configurations, and root canal orifice cross-sections were used to compare the teeth. Width and thickness comparisons between paired teeth in the same individual were made by paired t-test (Wilcoxon Signed Rank Test for variables not normally distributed). An online randomization tool generated randomized pairs (independent of the individual/patient). Subsequently, an unpaired t-test (or Mann-Whitney U test for non-normally distributed parameters) and a correlation analysis were conducted. Canal configurations were classified according to preexisting classification schemes. The number and location of accessory canals and apical foramina were registered and compared. Results: Utilizing advanced imaging techniques and quantitative analyses, our study establishes that contralateral mandibular incisors exhibit a remarkable degree of symmetry in multiple morphological parameters, including length, canal width, and dentinal thicknesses The apical third showed a high degree of inter-variability for the contralateral pairs. The rigorous statistical analysis of the normalized parameters by Z score showed no statistically significant differences between the contralateral mandibular incisors. Comparisons between central and lateral teeth revealed differences in root length but no significant disparity in the distribution of accessory canals. Central teeth, on average, were longer, while accessory canals were distributed relatively evenly between central and lateral teeth. Conclusions: The findings of this study further establish the significant similarities between contralateral mandibular incisors, reinforcing their suitability as a reliable substrate for root canal comparison studies. Clinical Relevance: The absence of statistically significant differences between contralateral pairs in normalized parameters underscores their potential as a reliable reference point for root canal comparison studies in clinical dentistry. Furthermore, our findings emphasize the importance of individualized treatment planning, considering the natural symmetry in mandibular incisors to enhance clinical decision-making. This research contributes valuable insights to the field of endodontics, offering a standardized approach to sample selection and enriching the understanding of dental anatomy
Abadpour, Shadab; Niemi, Essi Maria; Orrhult, Linnea Strid; Hermanns, Carolin; Vries, Rick de & Nogueira, Liebert Parreiras [Vis alle 12 forfattere av denne artikkelen] (2023). Adipose-Derived Stromal Cells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold. Advanced Healthcare Materials. ISSN 2192-2640. 12(32). doi: 10.1002/adhm.202300640. Fulltekst i vitenarkiv Vis sammendrag
Intra-portal islet transplantation is currently the only clinically approved beta cell replacement therapy, but its outcome is hindered by limited cell survival due to a multifactorial reaction against the allogeneic tissue in liver. Adipose-derived stromal cells (ASCs) can potentially improve the islet micro-environment by their immunomodulatory action. The challenge is to combine both islets and ASCs in a relatively easy and consistent long-term manner in a deliverable scaffold. W e report on manufacturing the Three-dimensional (3D) bioprinted double-layered scaffolds with primary islets and ASCs using a mix of alginate/nano-fibrillated cellulose (NFC) bioink. W e demonstrate the diffusion properties of the bioink and the supportive effect of human ASCs on islet viability, glucose sensing, insulin secretion and reducing the secretion of pro-inflammatory cytokines. Diabetic mice transplanted with islet-ASC scaffolds reached normoglycemia 7 days post transplantation with no significant difference between this group and the group received islets under the kidney capsules. In addition, animals transplanted with islet-ASC scaffolds stayed normoglycemic and showed elevated levels of C-peptide compared to mice transplanted with islet-only scaffolds. Our data presents a functional 3D bioprinted scaffold for islets and ASCs transplanted to the extrahepatic site and suggest a possible role of ASCs on improving the islet micro-environment.
Souza, Thiago Delgado de; Enrique-Leon, Alexis Jair; Aragão, Francisco Thiago Sacramento; Pereira, André Maués Brabo & Nogueira, Liebert Parreiras (2023). Advancements and applications of X-ray microcomputed tomography and digital image processing for the characterization of asphaltic materials. Transportes. ISSN 2237-1346. doi: 10.58922/transportes.v31i1.2854. Fulltekst i vitenarkiv Vis sammendrag
Este artigo apresenta os avanços recentes da aplicação da técnica de microtomografia computadorizada de raios X (micro-TC) na caracterização de materiais asfálticos. São discutidas as características de aquisição das imagens para realizar ensaios de micro-TC de concretos asfálticos e matrizes de agregados finos. Um procedimento desenvolvido para realizar o processamento digital das imagens dos materiais asfálticos também é apresentado. As principais conclusões deste artigo são: (1) resoluções espaciais entre 10 µm/pixel e 13 µm/pixel são adequadas para realizar a avaliação da volumetria dos materiais asfálticos; (2) em vez da limiarização, a arquitetura U-Net pode ser utilizada para otimizar o processamento digital de imagens; (3) um elemento de volume representativo considerando 33% do volume total das amostras pode ser adotado para avaliações da volumetria de materiais asfálticos; (4) a volumetria da matriz de agregados finos é dependente da volumetria da mistura asfáltica correspondente.
Pinelli, Filippo; Ponti, Maddalena; Delleani, Sara; Pizzetti, Fabio; Vanoli, Valeria & Vangosa, Francesco Briatico [Vis alle 12 forfattere av denne artikkelen] (2023). β-Cyclodextrin functionalized agarose-based hydrogels for multiple controlled drug delivery of ibuprofen. International Journal of Biological Macromolecules. ISSN 0141-8130. 252. doi: 10.1016/j.ijbiomac.2023.126284. Fulltekst i vitenarkiv
Larsen, Bjarke Strøm; Kissi, Eric Ofosu; Nogueira, Liebert Parreiras; Genina, Natalja & Tho, Ingunn (2023). Impact of drug load and polymer molecular weight on the 3D microstructure of printed tablets. Journal of Pharmaceutical Sciences. ISSN 0022-3549. 192. doi: 10.1016/j.ejps.2023.106619. Fulltekst i vitenarkiv Vis sammendrag
<p>This study investigates the influence of drug load and polymer molecular weight on the structure of tablets three-dimensionally (3D) printed from the binary mixture of prednisolone and hydroxypropyl methylcellulose (HPMC). Three different HPMC grades, (AFFINISOLTM HPMC HME 15LV, 90 Da (HPMC 15LV); 100LV, 180 Da (HPMC 100LV); 4M, 500 Da (HPMC 4M)), which are suitable for hot-melt extrusion (HME), were used in this study. HME was used to fabricate feedstock material, i.e., filaments, at the lowest possible extrusion temperature. Filaments of the three HPMC grades were prepared to contain 2.5, 5, 10 and 20 % (w/w) prednisolone. The thermal degradation of the filaments was studied with thermogravimetric analysis, while solid-state properties of the drug-loaded filaments were assessed with the use of X-ray powder diffraction. Prednisolone in the freshly extruded filaments was determined to be amorphous for drug loads up to 10%. It remained physically stable for at least 6 months of storage, except for the filament containing 10% drug with HPMC 15LV, where recrystallization of prednisolone was detected. <p>Fused deposition modeling was utilized to print honeycomb-shaped tablets from the HME filaments of HPMC 15LV and 100LV. The structural characteristics of the tablets were evaluated using X-ray microcomputed tomography, specifically porosity and size of structural elements were investigated. The tablets printed from HPMC 15LV possessed in general lower total porosity and pores of smaller size than tablets printed from the HPMC 100LV. The studied drug loads were shown to have minor effect on the total porosity of the tablets, though the lower the drug load was, the higher the variance of porosity along the height of the tablet was observed. It was found that tablets printed with HPMC 15LV showed higher structural similarity with the virtually designed model than tablets printed from HPMC 100LV. These findings highlight the relevance of the drug load and polymer molecular weight on the microstructure and structural properties of 3D printed tablets.
Yang, Yang; Pullisaar, Helen; Stunes, Astrid Kamilla; Nogueira, Liebert Parreiras; Syversen, Unni & Reseland, Janne Elin (2023). Irisin reduces orthodontic tooth movement in rats by promoting the osteogenic potential in the periodontal ligament. European Journal of Orthodontics. ISSN 0141-5387. 45(6), s. 842–853. doi: 10.1093/ejo/cjad021. Fulltekst i vitenarkiv Vis sammendrag
Objectives Positive effects of irisin on osteogenic differentiation of periodontal ligament (PDL) cells have been identified previously, this study aims to examine its effect on orthodontic tooth movement (OTM) in vivo. Materials and methods The maxillary right first molars of male Wistar rats (n = 21) were moved mesially for 14 days, with submucosal injection of two dosages of irisin (0.1 or 1 μg) or phosphate-buffered saline (control) every third day. OTM was recorded by feeler gauge and micro-computed tomography (μCT). Alveolar bone and root volume were analysed using μCT, and plasma irisin levels by ELISA. Histological characteristics of PDL tissues were examined, and the expression of collagen type I, periostin, osteocalcin (OCN), von Willebrand factor (vWF) and fibronectin type III domain-containing protein 5 (FNDC5) in PDL was evaluated by immunofluorescence staining. Results Repeated 1 μg irisin injections suppressed OTM on days 6, 9, and 12. No significant differences were observed in OTM in the 0.1 μg irisin group, or in bone morphometric parameters, root volume or plasma irisin, compared to control. Resorption lacunae and hyalinization were found at the PDL-bone interface on the compression side in the control, whereas they were scarce after irisin administration. The expression of collagen type I, periostin, OCN, vWF, and FNDC5 in PDL was enhanced by irisin administration. Limitations The feeler gauge method may overestimate OTM. Conclusions Submucosal irisin injection reduced OTM by enhancing osteogenic potential of PDL, and this effect was more significant on the compression side.
Pinelli, Filippo; Piras, Chiara; Nogueira, Liebert Parreiras & Rossi, Filippo (2023). On the Sorbent Ability and Reusability of Graphene-Oxide–Chitosan Aerogels for the Removal of Dyes from Wastewater. Gels. 9(2). doi: 10.3390/gels9020110. Fulltekst i vitenarkiv Vis sammendrag
One of the most persistent issues affecting people worldwide is water contamination due to the indiscriminate disposal of pollutants, causing severe environmental problems. Dyes are among the most harmful contaminants because of their high chemical stability and consequently difficult degradation. To remove contaminants from water, adsorption is the most widely used and effective method. In this work, we recall the results already published about the synthesis, the characterization and the use of porous graphene-oxide–chitosan aerogels as a sorbent material. Those systems, prepared by mixing GO sheets and CS chains, using APS as a cross-linking agent, and by further lyophilization, were further characterized using nano-computed tomography, supplying more understanding about their micro and nano-structure. Their sorbent ability has been investigated also by the study of their isotherm of adsorption of two different anionic dyes: Indigo Carmine and Cibacron Brilliant Yellow. Those analyses confirmed the potentialities of the aerogels and their affinity for those anionic dyes. Moreover, the possibility of regenerating and reusing the material was evaluated as a key aspect for applications of this kind. The treatment with NaOH, to promote the desorption of adsorbed dyes, and subsequent washing with HCl, to re-protonate the system, ensured the regeneration of the gels and their use in multiple cycles of adsorption with the selected water contaminants.
Sena, Gabriela; Silva, Ademir X. da; Nogueira, Liebert Parreiras; Colaço, Marcos Vinicius; Metscher, Brian & Fidalgo, Gabriel [Vis alle 11 forfattere av denne artikkelen] (2023). Investigation of Rhodnius prolixus head anatomy using microtomography. Journal of Microscopy. ISSN 0022-2720. 290(3), s. 161–167. doi: 10.1111/jmi.13186. Fulltekst i vitenarkiv
Mosti, Mats Peder; Stafne, Signe; Sujan, Md Abu Jafar; Redalen, Kathrine Røe; Marthinsen, Anne Beate Langeland & Skallerud, Bjørn Helge [Vis alle 12 forfattere av denne artikkelen] (2022). Strength training reduces radiation-induced bone deterioration and maintains lean mass in a rat model. Bone Reports. 16, s. 101369–101369. doi: 10.1016/j.bonr.2022.101369. Fulltekst i vitenarkiv
Partenope, Alessandra; Pizzetti, Fabio; Vanoli, Valeria; Casalegno, Mosè; Cingolani, Alberto & Nogueira, Liebert Parreiras [Vis alle 9 forfattere av denne artikkelen] (2022). A facile surfactant-free strategy to construct porous structures with hydrophobic and hydrophilic domains from polymer/water mixtures. Materials Today Communications. 33. doi: 10.1016/j.mtcomm.2022.104290. Fulltekst i vitenarkiv
Luzon, Javier; Thorsen, Yngve; Nogueira, Liebert Parreiras; Andersen, Solveig Norheim; Edwin, Bjørn von Gohren & Haugen, Håvard Jostein [Vis alle 8 forfattere av denne artikkelen] (2022). Reconstructing topography and extent of injury to the superior mesenteric artery plexus in right colectomy with extended D3 mesenterectomy: a composite multimodal 3-dimensional analysis. Surgical Endoscopy. ISSN 0930-2794. 36(10), s. 7607–7618. doi: 10.1007/s00464-022-09200-2. Fulltekst i vitenarkiv Vis sammendrag
Background Superior mesenteric artery plexus (SMAP) injury is reported to cause postoperative intractable diarrhea after pancreatic/colonic surgery with extended lymphadenectomy. This study aims to describe the SMAP microanatomy and extent of injury after right colectomy with extended D3 mesenterectomy for cancer. Methods Three groups (I) anatomical dissection, (II) postmortem histology, and (III) surgical specimen histology were included. Nerve count and area were compared between groups II and III and paravascular sheath thickness between groups I and II. 3D models were generated through 3D histology, nanoCT scanning, and finally through 3D printing. Results A total of 21 specimens were included as follows: Group (I): 5 (3 females, 80–93 years), the SMAP is a complex mesh surrounding the superior mesenteric artery (SMA), branching out, following peripheral arteries and intertwining between them, (II): 7 (5 females, 71–86 years), nerve count: 53 ± 12.42 (38–68), and area: 1.84 ± 0.50 mm2 (1.16–2.29), and (III): 9 (5 females, 55–69 years), nerve count: 31.6 ± 6.74 (range 23–43), and area: 0.889 ± 0.45 mm2 (range 0.479–1.668). SMAP transection injury is 59% of nerve count and 48% of nerve area at middle colic artery origin level. The median values of paravascular sheath thickness decreased caudally from 2.05 to 1.04 mm (anatomical dissection) and from 2.65 to 1.17 mm (postmortem histology). 3D histology models present nerve fibers exclusively within the paravascular sheath, and lymph nodes were observed only outside. NanoCT-derived models reveal oblique nerve fiber trajectories with inclinations between 35° and 55°. Two 3D-printed models of the SMAP were also achieved in a 1:2 scale. Conclusion SMAP surrounds the SMA and branches within the paravascular sheath, while bowel lymph nodes and vessels lie outside. Extent of SMAP injury on histological slides (transection only) was 48% nerve area and 59% nerve count. The 35°–55° inclination range of SMAP nerves possibly imply an even larger injury when plexus excision is performed (lymphadenectomy). Reasons for later improvement of bowel function in these patients can lie in the interarterial nerve fibers between SMA branches.
Johnsen, Gaute Floer; Haugen, Håvard Jostein; Nogueira, Liebert Parreiras; Sevgi, Usame; Jimenez, Ashley Mae & DeLuca, Joseph T. [Vis alle 8 forfattere av denne artikkelen] (2022). Assessment of the Root Canal Similarity in Contralateral Mandibular Incisors. International Dental Journal. ISSN 0020-6539. doi: 10.1016/j.identj.2022.04.003. Fulltekst i vitenarkiv Vis sammendrag
Introduction The purpose of this study was to determine the degree of similarity between contralateral mandibular incisors utilising 3-dimensional (3D) models obtained from micro-computed tomographic (micro-CT) scans of extracted human teeth. The null hypothesis was that contralateral mandibular incisors do not exhibit matching symmetry. Methods Sixty pairs (n = 120) of extracted mandibular incisors were obtained from 30 patients and scanned with micro-CT with a voxel size of 15.0 μm. 3D virtual models of the pulpal cavities were rendered. Geometric morphometric deviation analysis was performed after mirroring, automatic alignment, and co-registration of the models of contralateral teeth root mean square (RMS) errors were calculated. The quantitative analysis of the 3D models included 6 different geometric parameters. Data sets were examined with a 2-sample Kolmogorov–Smirnov test. Post hoc retrospective power analysis was performed to find statistical power (α = 0.05). Results Contralateral pairs had a narrower distribution in deviation than random pairs. Also, contralateral pairs showed a statistically higher similarity coefficient (5 out of 6 geometric parameters) compared to random pairs (P < .001); no difference was found when comparing central to lateral pairs or between Vertucci type I configurations compared to non-type I. RMS errors had significantly lower Contralateral premolars (CPs) values than random pairs (P < .001). Conclusions A high degree of similarity was demonstrated for pairing contralateral mandibular incisors using 3D models. The similarity between contralateral central and lateral incisors suggests that when screened and matched, these 4 teeth might be used in endodontic research where similar root canal anatomy is crucial.
Elkolali, Moustafa; Nogueira, Liebert Parreiras; Rønning, Per Ola & Alcocer, Alex (2022). Void Content Determination of Carbon Fiber Reinforced Polymers: A Comparison between Destructive and Non-Destructive Methods. Polymers. 14(6), s. 1–16. doi: 10.3390/polym14061212. Fulltekst i vitenarkiv Vis sammendrag
The properties of composite materials are highly dependent on the fiber and matrix fraction and on the porosity resulting from micro voids. This paper addresses void content characterization and the constituent content of composite materials by resorting to a comparison of destructive and non-destructive methods. The work presents the detailed procedures of two destructive methods, using acid digestion of epoxy resins matrices, and compares their processes. It also presents the results of a non-destructive method, by means of Micro Computed Tomography (MicroCT). The results of both destructive and non-destructive methods are compared, and a recommendation is made based on the application and the type of composite being analyzed. The MicroCT showed better and more consistent results in detecting voids in the material, while the acid digestion tests provided better results about the fiber and matrix percentage. Exported results from the MicroCT scanning with actual locations of voids were used in numerical analysis, to examine the feasibility of using them, whether by developing models that map damage in the proximity of the void, or by developing models that predict the properties of the entire material with respect to the content, shape, and distribution in the material.
Ribeiro, João P.; Domingues, Rui M.A.; Babo, Pedro S.; Nogueira, Liebert Parreiras; Reseland, Janne Elin & Reis, Rui L. [Vis alle 8 forfattere av denne artikkelen] (2022). Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate and biomineralized cellulose nanocrystals. Carbohydrate Polymers. ISSN 0144-8617. 292. doi: 10.1016/j.carbpol.2022.119638. Fulltekst i vitenarkiv
Santos, Jefferson Ferreira; Ferreira-Machada, Samara Cristina; Salata, Camila; Nogueira, Liebert Parreiras; Lau, Claudio Sergio Correa & Appoloni, Carlos Roberto [Vis alle 7 forfattere av denne artikkelen] (2022). RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis. Brazilian Journal of Health Review. ISSN 2595-6825. doi: 10.34119/bjhrv5n1-199. Fulltekst i vitenarkiv Vis sammendrag
Objective: Osteoradionecrosis (ORN) of the jaws is an important side effect of head and neck cancer radiotherapy. The main objective of this work was to evaluate the expression of RANK, RANKL and OPG proteins, markers of bone remodeling, in rats submitted to radiotherapy. Material and Methods: Six-month-old male Wistar rats were divided into two groups: Irradiated group (IR), which received a single dose of 20 Gy in the jaw and after seven days had their three left mandibular molars extracted; Control group (C), which didn’t receive radiation, but underwent the same procedures. Twenty one days after the dental extractions, the animals were euthanized and their mandibles were removed for analysis. The volume density (Vv) of immunoexpression of RANK/RANKL/OPG was quantified by stereology. The bone volume ratio (BV/TV), the trabecular thickness (Tb.Th), the trabecular separation (Tb.Sp) and the trabecular number (Tb.N) were analyzed by microtomography (Micro-CT). Results: Vv of immunoexpression of RANKL and OPG were, respectively, 53% higher and 50% lower in IR animals compared to C group (p<0.05). Micro-CT showed that in the IR animals there was a reduction of the bone mass compared to the C animals. BV/TV and Tb.Th were 33% and 38% lower, respectively, in the IR animals (p<0.05), while Tb.Sp was 37.5% higher in these animals (p<0.05). Conclusions: Ionizing irradiation in a single high dose, followed by dental extractions promoted ORN in mandibular bone in rats, together with changes in the markers of bone remodeling RANK and OPG.
Munir, Arooj; Marovic, Danijela; Nogueira, Liebert Parreiras; Simm, Roger; Naemi, Ali-Oddin & Landrø, Sander Marius [Vis alle 14 forfattere av denne artikkelen] (2022). Using Copper-Doped Mesoporous Bioactive Glass Nanospheres to Impart Anti-Bacterial Properties to Dental Composites. Pharmaceutics. 14(10). doi: 10.3390/pharmaceutics14102241. Fulltekst i vitenarkiv Vis sammendrag
Experimental dental resin composites containing copper-doped mesoporous bioactive glass nan-ospheres (Cu-MBGN) were developed to impart anti-bacterial properties. Increasing amounts of Cu-MBGN (0, 1, 5 and 10 wt%) were added to the BisGMA/TEGDMA resin matrix containing micro- and nano-fillers of inert glass, keeping the resin/filler ratio constant. Surface micromor-phology and elemental analysis were performed to evaluate the homogeneous distribution of filler particles. The study investigated the effects of Cu-MBGN on the degree of conversion, polymeri-zation shrinkage, porosity, ion release and anti-bacterial activity on S. mutans and A. naeslundii. Experimental materials containing Cu-MBGN showed a dose-dependent Cu release with an initial burst and a further increase after 28 days. The composite containing 10% Cu-MBGN had the best anti-bacterial effect on S. mutans, as evidenced by the lowest adherence of free-floating bacteria and biofilm formation. In contrast, the 45S5-containing materials had the highest S. mutans ad-herence. Ca release was highest in the bioactive control containing 15% 45S5, which correlated with the highest number of open porosities on the surface. Polymerization shrinkage was similar for all tested materials, ranging from 3.8 to 4.2%, while the degree of conversion was lower for Cu-MBGN materials. Cu-MBGN composites showed better anti-bacterial properties than compo-sites with 45S5 BG.
Morimoto, Juliano; Barcellos, Renan; Schoborg, Todd A.; Nogueira, Liebert Parreiras & Colaço, Marcos Vinicius (2022). Assessing Anatomical Changes in Male Reproductive Organs in Response to Larval Crowding Using Micro-computed Tomography Imaging. Neotropical Entomology. ISSN 1519-566X. 51(4), s. 526–535. doi: 10.1007/s13744-022-00976-5. Fulltekst i vitenarkiv Vis sammendrag
Abstract Ecological conditions shape (adaptive) responses at the molecular, anatomical, and behavioral levels. Understanding these responses is key to predict the outcomes of intra- and inter-specific competitions and the evolutionary trajectory of populations. Recent technological advances have enabled large-scale molecular (e.g., RNAseq) and behavioral (e.g., computer vision) studies, but the study of anatomical responses to ecological conditions has lagged behind. Here, we highlight the role of X-ray micro-computed tomography (micro-CT) in generating in vivo and ex vivo 3D imaging of anatomical structures, which can enable insights into adaptive anatomical responses to ecological environments. To demonstrate the application of this method, we manipulated the larval density of Drosophila melanogaster Meigen flies and applied micro-CT to investigate the anatomical responses of the male reproductive organs to varying intraspecific competition levels during development. Our data is suggestive of two classes of anatomical responses which broadly agree with sexual selection theory: increasing larval density led to testes and ejaculatory duct to be overall larger (in volume), while the volume of accessory glands and, to a lesser extent, ejaculatory duct decreased. These two distinct classes of anatomical responses might reflect shared developmental regulation of the structures of the male reproductive system. Overall, we show that micro-CT can be an important tool to advance the study of anatomical (adaptive) responses to ecological environments.
Bjørge, Isabel M.; Sousa, Bárbara M. De; Patrício, Sónia G.; Silva, Ana Sofia; Nogueira, Liebert Parreiras & Santos, Lúcia F. [Vis alle 10 forfattere av denne artikkelen] (2022). Bioengineered Hierarchical Bonelike Compartmentalized Microconstructs Using Nanogrooved Microdiscs. ACS Applied Materials & Interfaces. ISSN 1944-8244. 14(17), s. 19116–19128. doi: 10.1021/acsami.2c01161. Fulltekst i vitenarkiv
Wang, Zhule; Nogueira, Liebert Parreiras; Haugen, Håvard Jostein; Geest, Ingrid C M Van Der; Ro, Patricia Caetano de Almeida & Janssen, Dennis [Vis alle 9 forfattere av denne artikkelen] (2021). Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells. Bioactive Materials. doi: 10.1016/j.bioactmat.2021.12.023. Fulltekst i vitenarkiv
Torben, Hildebrand; Nogueira, Liebert Parreiras; Sunde, Pia Titterud; Ørstavik, Dag; Glasmacher, Birgit & Haugen, Håvard Jostein (2021). Contrast-enhanced nano-CT reveals dental soft tissues and cellular layers. International Endodontic Journal. ISSN 0143-2885. 54(8), s. 1275–1288. doi: 10.1111/iej.13527. Fulltekst i vitenarkiv Vis sammendrag
Aim: Nano-CT technologies offer 3D imaging methods that allow high-resolution examination of bones and teeth, but soft tissue components have weak X-ray attenuation and are not easily visualised in CT images. We introduce a methodology designed to simultaneously visualise dental ultrastructure, including cellular and soft tissue components, by utilising phosphotungstic acid (PTA) as a contrast-enhancement agent. Methodology: Sound third molars were collected from healthy human adults and fixed in 4% buffered paraformaldehyde. To evaluate the impact of PTA in concentrations of 0.3%, 0.7% and 1% on dental soft and hard tissues for CT imaging, cementum and dentine-pulp sections were cut, dehydrated and stained with immersion periods of 12 hours, 24 hours, 2 days or 5 days. The samples were scanned with high-resolution nano-CT, where we examined both the cementum and pulpal regions with pixel sizes down to 0.5 µm for dental-pulp sections. Results: Dental cementum and periodontium as well as odontoblasts and predentine were made visible through PTA-staining in high-resolution three-dimensional nano-CT scans. Different segments of the tooth required different staining protocols. The thickness of the cementum could be computed over the height of the tooth once it was made visible by the PTA-enhanced contrast, and the attached soft tissue components of the interior of the tooth could be shown on the dentine-pulp interface in greater detail. Three-dimensional illustrations allowed a histology-like visualisation of the sections in all orientations with a single scan and easy sample preparation. The segmentation of the sigmoidal dentinal tubules and the surrounding dentine allowed a three-dimensional investigation and quantative of the dentine composition, such as the tubular lumen or the ratio of the tubular lumen area to the dentinal surface. Conclusion: The staining protocol made it possible to visualise hard tissues along with cellular layers and soft tissues using a laboratory-based nano-CT technique. The protocol depended on both tissue type and size. This methodology offers enhanced possibilities for the concomitant visualisation of soft and hard dental tissues.
Zhu, Hao; Haugen, Håvard Jostein; Perale, Giuseppe; Reseland, Janne Elin; Nogueira, Liebert Parreiras & Cantalapiedra, Antonio gonzalez [Vis alle 11 forfattere av denne artikkelen] (2021). Tailoring Resorption Rates and Osteogenic Response in Xeno-Hybrid Bone Grafts: The Effect of Added Gelatins. Engineering (Beijing). ISSN 2095-8099. 13, s. 197–208. doi: 10.1016/j.eng.2021.01.010. Fulltekst i vitenarkiv Vis sammendrag
Bone defects resulting from trauma, surgery, congenital malformations, and other factors are among the most common health problems nowadays. Although current strategies such as autografts and allografts are recognized as the most successful treatments for stimulating bone regeneration, limitations such as graft source and complications still exist. SmartBone® is a xeno-hybrid bone graft (made from bovine bone matrix, poly(L-lactic-co-ε-caprolactone), and gelatin) with a positive clinical record for bone regeneration. In this study, the formulation for designing xeno-hybrid bone grafts using gelatins from different sources (bovine- and porcine-derived gelatin, with bone grafts named SBN and SPK, respectively) was investigated, and the biological responses were evaluated in vitro and in vivo. The results demonstrate that gelatins from both bovine and porcine sources can be loaded onto SmartBone® successfully and safely, withstanding the aggressive manufacturing processes. Different bone cell responses were observed in vitro. SBN was found to enhance osteocalcin secretion while SPK was found to upregulate osteopontin from human osteoblasts. In vivo, both bone grafts promoted osteogenesis, but SPK degraded earlier than SBN. Our findings suggest that SBN and SPK provide different yet comparable solutions for optimizing the bone resorption and regeneration balance. These xeno-hybrid bone grafts possess ideal potential for bone defect repairing.
Enriquez, J. J. S.; Sena, Gabriela; Cataldi, Marco; Silveira, L.H.M. da; Braz, Delson & Barroso, Regina C. [Vis alle 12 forfattere av denne artikkelen] (2021). The Methodology to produce Rhodnius prolixus head structures made by 3D printing using Synchrotron Radiation Microtomography. Journal of Instrumentation (JINST). doi: 10.1088/1748-0221/16/02/P02014. Fulltekst i vitenarkiv
Colaço, Marcos; Camara, Alexandre Resende; Nogueira, Liebert Parreiras; Carvalho, Isabel C. S.; Margulis, Walter & Barroso, Regina C. (2021). Characterization of microstructures of specialty optical fibers for electric field sensing by propagation-based X-ray phase-contract tomography. Measurement Science and Technology. ISSN 0957-0233. doi: 10.1088/1361-6501/abd365. Fulltekst i vitenarkiv Vis sammendrag
In this work, we present a quantitative (statistical) 3D morphological characterization of optical fibers used in electric field sensing. The characterization technique employs propagation-based X-ray phase-contrast microcomputed tomography (micro-CT). Particularly, we investigate specialty optical fibers that contain microstructured holes that are electrooptically modified by thermal poling to induce second-order nonlinear effects (SONE). The efficiency of SONE is reflected in the characterization parameter Vπ, which is highly dependent on the dimensions of the fiber. The fiber microstructures must be uniform to allow the fabrication of reproducible devices. The results obtained using the micro-CT technique showed that an uncertainty of ±1.7% arises in the determination of the expected value of the voltage that causes a change in the phase of the electromagnetic wave equal to π rad (Vπ), showing a great advantage when it is compared with other techniques e.g. SEM, which would need at least 1000 images of the cross-section of the optical fiber taken in different points making the process more expensive and time consuming.
Azevedo, Pedro L.; Dias, Rhayara; Nogueira, Liebert Parreiras; Maradei, Simone; Bigni, Ricardo & Aragão, Jordana S. R. [Vis alle 8 forfattere av denne artikkelen] (2021). Reduced Osteogenic Differentiation Potential In Vivo in Acute Myeloid Leukaemia Patients Correlates with Decreased BMP4 Expression in Mesenchymal Stromal Cells. International Journal of Stem Cells. ISSN 2005-3606. 15(2), s. 227–232. doi: 10.15283/ijsc21138. Fulltekst i vitenarkiv Vis sammendrag
The osteogenic differentiation potential of mesenchymal stromal cells (hMSCs) is an essential process for the haematopoiesis and the maintenance of haematopoietic stem cells (HSCs). Therefore, the aim of this work was to evaluate this potential in hMSCs from AML patients (hMSCs-AML) and whether it is associated with BMP4 expression. The results showed that bone formation potential in vivo was reduced in hMSCs-AML compared to hMSCs from healthy donors (hMSCs-HD). Moreover, the fact that hMSCs-AML were not able to develop supportive haematopoietic cells or to differentiate into osteocytes suggests possible changes in the bone marrow microenvironment. Furthermore, the expression of BMP4 was decreased, indicating a lack of gene expression committed to the osteogenic lineage. Overall, these alterations could be associated with changes in the maintenance of HSCs, the leukaemic transformation process and the development of AML.
Léon, Alexis Jair Enríquez; Souza, Thiago Delgado; Aragão, Francisco thiago sacramento; Pereira, André Maués Brabo & Nogueira, Liebert Parreiras (2021). Characterization of the air void content of fine aggregate matrices within asphalt concrete mixtures. Construction and Building Materials. ISSN 0950-0618. doi: 10.1016/j.conbuildmat.2021.124214. Fulltekst i vitenarkiv
Kissi, Eric Ofosu; Nilsson, Robin; Nogueira, Liebert Parreiras; Larsson, Anette & Tho, Ingunn (2021). Influence of Drug Load on the Printability and Solid-State Properties of 3D-Printed Naproxen-Based Amorphous Solid Dispersion. Molecules. ISSN 1431-5157. 26(15). doi: 10.3390/molecules26154492. Fulltekst i vitenarkiv Vis sammendrag
Fused deposition modelling-based 3D printing of pharmaceutical products is facing challenges like brittleness and printability of the drug-loaded hot-melt extruded filament feedstock and stabilization of the solid-state form of the drug in the final product. The aim of this study was to investigate the influence of the drug load on printability and physical stability. The poor glass former naproxen (NAP) was hot-melt extruded with Kollidon® VA 64 at 10–30% w/w drug load. The extrudates (filaments) were characterised using differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), and thermogravimetric analysis (TGA). It was confirmed that an amorphous solid dispersion was formed. A temperature profile was developed based on the results from TGA, DSC, and DMA and temperatures used for 3D printing were selected from the profile. The 3D-printed tablets were characterised using DSC, X-ray computer microtomography (XµCT), and X-ray powder diffraction (XRPD). From the DSC and XRPD analysis, it was found that the drug in the 3D-printed tablets (20 and 30% NAP) was amorphous and remained amorphous after 23 weeks of storage (room temperature (RT), 37% relative humidity (RH)). This shows that adjusting the drug ratio can modulate the brittleness and improve printability without compromising the physical stability of the amorphous solid dispersion.
Léon, Alexis Jair Enríquez; Souza, Thiago Delgado; Aragão, Francisco thiago sacramento; Braz, Delson; Pereira, André Maués Brabo & Nogueira, Liebert Parreiras (2021). Determination of the air void content of asphalt concrete mixtures using artificial intelligence techniques to segment micro-CT images. International Journal of Pavement Engineering. ISSN 1029-8436. doi: 10.1080/10298436.2021.1931197. Fulltekst i vitenarkiv
Rocha, Elisson BD da; Souza, Ana Maria F; Silva, Ana Lúcia N; Furtado, Cristina RG; Colaço, Marcos Vinicius & Nogueira, Liebert Parreiras (2021). Quantitative micro-computed tomography analysis and rheological investigation of Nitrile rubber/Rockwool composites. Journal of composite materials. ISSN 0021-9983. doi: 10.1177/00219983211033886. Fulltekst i vitenarkiv
Zhu, Hao; Li, Meng; Huang, Xiaolong; Qi, Dahu; Nogueira, Liebert Parreiras & Yuan, Xi [Vis alle 11 forfattere av denne artikkelen] (2021). 3D printed tricalcium phosphate-bioglass scaffold with gyroid structure enhance bone ingrowth in challenging bone defect treatment. Applied Materials Today. ISSN 2352-9407. doi: 10.1016/j.apmt.2021.101166. Fulltekst i vitenarkiv
Salata, Camila; Almeida, Carlos Eduardo de; Ferreira-Machado, Samara C.; Barroso, Regina C.; Nogueira, Liebert Parreiras & Mantuano, Andrea [Vis alle 9 forfattere av denne artikkelen] (2021). Preliminary Pre-Clinical studies on the side effects of breast cancer treatment. International Journal of Radiation Biology. ISSN 0955-3002. doi: 10.1080/09553002.2021.1919782. Fulltekst i vitenarkiv Vis sammendrag
Technological advancement in the treatment of cancer together with early detection and diagnosis have considerably improved the survival of breast cancer patients. On the other hand, the potential of patients developing side effects from cancer treatment are not negligible. Despite the progress that has been made in terms of early diagnosis, therapy, and survival, including improvements in the chemotherapeutic agents, radiation and molecular targeted therapies, cardiotoxicity of cancer therapy is still cause for concern. Radiation therapy for breast cancer is associated with increased risk of heart disease and myocardial infarction. Furthermore, the association of radiation therapy to chemotherapy is an important aspect to be considered in the development of cardiac disease, as this could play an additional role as a risk factor. Besides the heart effect, other side effects can be observed in the bone, ovary, uteri, and other organs. This paper aims to review the recent literature to present the current understanding of side effects associated with breast cancer treatment. The focus is on recent preclinical studies that have assessed potential changes in different organs that may be injured after breast cancer treatment, both due to both radiation and chemotherapy agents. Conclusion: Radiation-induced heart disease is one important side effect that must be considered during the treatment planning and patient follow-up. The cardiac damage can be potentialized when chemotherapy is associated to radiotherapy, and the literature findings indicate that heart fibrosis plays an important role at the radio-chemotherapy induced cardiac damage. Literature findings also showed important side effects at the bone, that can lead to ospeoporosis, due to the decrease of calcium, after radio or chemotherapy treatments. This decrease could be explained by the ovarian failure observed at rats after chemotherapy treatment. It is of great importance to acknowledge the complications originating from the treatment, so that new strategies can be developed. In this way, it will be possible to minimize side effects and improve the patients’ quality of life.
Sena, Gabriela; Barroso, Regina C.; Braz, Delson; Nogueira, Liebert Parreiras; Colaço, Marcos & Kourousias, George [Vis alle 21 forfattere av denne artikkelen] (2021). Evaluation of the effects of Azadirachtin on internal structures of Rhodnius prolixus head using low-energy X-ray microfluorescence. Spectrochimica Acta Part B - Atomic Spectroscopy. ISSN 0584-8547. doi: 10.1016/j.sab.2020.106064. Fulltekst i vitenarkiv
Haugen, Håvard Jostein; Qasim, Syed Saad Bin; Matinlinna, Jukka P; Vallittu, Pekka K. & Nogueira, Liebert Parreiras (2020). Nano-CT as tool for characterization of dental resin composites. Scientific Reports. 10. doi: 10.1038/s41598-020-72599-y. Fulltekst i vitenarkiv Vis sammendrag
Technological advances have made it possible to examine dental resin composites using 3D nanometer resolution. This investigation aims to characterize existing dental nano-hybrid and micro-hybrid resin composites through comparing and contrasting nano-computed tomography (nano-CT) with micro-CT and high-resolution SEM images. Eight commercially available and widely used dental resin composites, 2 micro-hybrid and 6 nano-hybrid were researched. Cured samples were examined and characterized using nano-CT (resolution 450 nm) and compared with micro-CT images (resolution 2 µm). Acquired images were reconstructed and image analysis was carried out to determine porosity and pore morphology. A comprehensive comparison of scanning micrograph images unsurprisingly revealed that the nano-CT images displayed greater detail of the ultrastructure of cured dental resin composites. Filler particle diameters and its volumes were lower when measured using nano-CT, porosity being higher where analysed at higher resolution. There were large variations between the examined materials. Fewer voids were found in Tetric EvoCeram and IPS Empress Direct, the smallest pores being found in Universal XTE and Tetric EvoCeram. Nano-CT was successfully used to investigate the morphology of dental resin composites and showed that micro-CT gives a lower porosity and pore size but overestimates filler particle size. There were large discrepancies between the tested composites. Evidence of porosities and pores within a specimen is a critical finding and it might have a detrimental effect on a material’s clinical performance.
Daood, Umer; Omar, Hanan; Qasim, Saad B; Nogueira, Liebert Parreiras; Pichika, Malikarjuna & Mak, Kit-Kay [Vis alle 10 forfattere av denne artikkelen] (2020). New antimicrobial and collagen crosslinking formulated dentin adhesive with improved bond durability. Journal of The Mechanical Behavior of Biomedical Materials. ISSN 1751-6161. doi: 10.1016/j.jmbbm.2020.103927. Fulltekst i vitenarkiv Vis sammendrag
Objective Here we describe a novel formulation, based on quaternary ammonium (QA) and riboflavin (RF), which combines antimicrobial activities and protease inhibitory properties with collagen crosslinking without interference to bonding capabilities, was investigated. Methods Experimental adhesives modified with different fractions of dioctadecyldimethyl ammonium bromide quaternary ammonium and riboflavin (QARF) were formulated. Dentine specimens were bonded to resincomposites with control or the experimental adhesives to be evaluated for bond strength, interfacial morphology, micro-Raman analysis, nano-CT and nano-leakage expression. In addition, the antibacterial and biocompatibilities of the experimental adhesives were investigated. The endogenous proteases activities and their molecular binding-sites were studied. Results Modifying the experimental adhesives with QARF did not adversely affect micro-tensile bond strength or the degree of conversion along with the demonstration of anti-proteases and antibacterial abilities with acceptable biocompatibilities. In general, all experimental adhesives demonstrated favourable bond strength with increased and improved values in 1% QARF adhesive at 24 h (39.2 ± 3.0 MPa) and following thermocycling (34.8 ± 4.3 MPa). Significance It is possible to conclude that the use of QARF with defined concentration can maintain bond strength values when an appropriate protocol is used and have contributed in ensuring a significant decrease in microbial growth of biofilms. Incorporation of 1% QARF in the experimental adhesive lead to simultaneous antimicrobial and anti-proteolytic effects with low cytotoxic effects, acceptable bond strength and interfacial morphology.
Qasim, Saad B; Nogueira, Liebert Parreiras; S, fawzy Amr & Daood, Umer (2020). The Effect of Cross-linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regeneration. AAPS PharmSciTech. doi: 10.1208/s12249-020-01708-x. Fulltekst i vitenarkiv Vis sammendrag
Innovative strategies for periodontal regeneration have been the focus of research clusters across the globe for decades. In order to overcome the drawbacks of currently available options, investigators have suggested a novel concept of functionally graded membrane (FGM) templates with different structural and morphological gradients. Chitosan (CH) has been used in the past for similar purpose. However, the composite formulation of composite and tetracycline when cross-linked with glutaraldehyde have received little attention. Therefore, the purpose of the study was to investigate the drug loading and release characteristics of novel freeze gelated chitosan templates at different percentages of glutaraldehyde. These were cross-linked with 0.1 and 1% glutaraldehyde and loaded with doxycycline hyclate. The electron micrographs depicted porous morphology of neat templates. After cross-linking, these templates showed compressed ultrastructures. Computerized tomography analysis showed that the templates had 88 to 92% porosity with average pore diameter decreased from 78 to 44.9 μm with increasing concentration. Fourier transform infrared spectroscopy showed alterations in the glycosidic segment of chitosan fingerprint region which after drug loading showed a dominant doxycycline spectral composite profile. Interestingly, swelling profile was not affected by cross-linking either at 0.1 and 1% glutaraldehyde and template showed a swelling ratio of 80%, which gained equilibrium after 15 min. The drug release pattern also showed a 40 μg/mL of release after 24 h. These doxycycline-loaded templates show their tendency to be used in a functionally graded membrane facing the defect site.
Osmari, Patrícia Hennig; Costa, Rogério Ferreira da; Aragão, Francisco Thiago Sacramento; Braz, Delson; Barroso, Regina C. & Nogueira, Liebert Parreiras [Vis alle 7 forfattere av denne artikkelen] (2020). Determination of Volumetric Characteristics of FAM Mixtures using X-Ray Micro-Computed Tomography and Their Effects on the Rheological Behavior of the Material. Transportation Research Record. ISSN 0361-1981. doi: 10.1177/0361198120914607. Fulltekst i vitenarkiv
Fidalgo, Gabriel; Paiva, Katrine; Mendes, Gabriela; Barcellos, Renan; Colaço, Gustavo & Sena, Gabriela [Vis alle 14 forfattere av denne artikkelen] (2020). Synchrotron microtomography applied to the volumetric analysis of internal structures of Thoropa miliaris tadpoles. Scientific Reports. 10. doi: 10.1038/s41598-020-75993-8. Fulltekst i vitenarkiv Vis sammendrag
Amphibians are models for studying applied ecological issues such as habitat loss, pollution, disease, and global climate change due to their sensitivity and vulnerability to changes in the environment. Developmental series of amphibians are informative about their biology, and X-ray based 3D reconstruction holds promise for quantifying morphological changes during growth—some with a direct impact on the possibility of an experimental investigation on several of the ecological topics listed above. However, 3D resolution and discrimination of their soft tissues have been difficult with traditional X-ray computed tomography, without time-consuming contrast staining. Tomographic data were initially performed (pre-processing and reconstruction) using the open-source software tool SYRMEP Tomo Project. Data processing and analysis of the reconstructed tomography volumes were conducted using the segmentation semi-automatic settings of the software Avizo Fire 8, which provide information about each investigated tissues, organs or bone elements. Hence, volumetric analyses were carried out to quantify the development of structures in different tadpole developmental stages. Our work shows that synchrotron X-ray microtomography using phase-contrast mode resolves the edges of the internal tissues (as well as overall tadpole morphology), facilitating the segmentation of the investigated tissues. Reconstruction algorithms and segmentation software played an important role in the qualitative and quantitative analysis of each target structure of the Thoropa miliaris tadpole at different stages of development, providing information on volume, shape and length. The use of the synchrotron X-ray microtomography setup of the SYRMEP beamline of Elettra Synchrotron, in phase-contrast mode, allows access to volumetric data for bone formation, eye development, nervous system and notochordal changes during the development (ontogeny) of tadpoles of a cycloramphid frog Thoropa miliaris. As key elements in the normal development of these and any other frog tadpole, the application of such a comparative ontogenetic study, may hold interest to researchers in experimental and environmental disciplines.
Sena, Gabriela; Nogueira, Liebert Parreiras; Braz, Delson; Colaço, Marcos V.; Almeida, Andre P. & Azambuja, Patricia [Vis alle 9 forfattere av denne artikkelen] (2019). Improving Image Quality of Rhodnius prolixus head using different types of staining methods and Synchrotron Radiation Phase Contrast Microtomography. Radiation Physics and Chemistry. ISSN 0969-806X. 155, s. 26–30. doi: 10.1016/j.radphyschem.2018.06.039. Fulltekst i vitenarkiv
Dias, Rhayra B.; Guimaraes, Joao A. M.; Cury, Marco B.; Rocha, Leonardo R.; Costa, Elaine S. da & Nogueira, Liebert Parreiras [Vis alle 14 forfattere av denne artikkelen] (2019). The manufacture of GMP-grade bone marrow stromal cells with validated in vivo bone-forming potential in an orthopedic clinical center in Brazil. Stem Cells International. ISSN 1687-966X. 2019:2608482, s. 1–18. doi: 10.1155/2019/2608482. Fulltekst i vitenarkiv Vis sammendrag
In vitro-expanded bone marrow stromal cells (BMSCs) have long been proposed for the treatment of complex bone-related injuries because of their inherent potential to differentiate into multiple skeletal cell types, modulate inflammatory responses, and support angiogenesis. Although a wide variety of methods have been used to expand BMSCs on a large scale by using good manufacturing practice (GMP), little attention has been paid to whether the expansion procedures indeed allow the maintenance of critical cell characteristics and potency, which are crucial for therapeutic effectiveness. Here, we described standard procedures adopted in our facility for the manufacture of clinical-grade BMSC products with a preserved capacity to generate bone in vivo in compliance with the Brazilian regulatory guidelines for cells intended for use in humans. Bone marrow samples were obtained from trabecular bone. After cell isolation in standard monolayer flasks, BMSC expansion was subsequently performed in two cycles, in 2- and 10-layer cell factories, respectively. The average cell yield per cell factory at passage 1 was of 21.93±12.81×106 cells, while at passage 2, it was of 83.05±114.72×106 cells. All final cellular products were free from contamination with aerobic/anaerobic pathogens, mycoplasma, and bacterial endotoxins. The expanded BMSCs expressed CD73, CD90, CD105, and CD146 and were able to differentiate into osteogenic, chondrogenic, and adipogenic lineages in vitro. Most importantly, nine out of 10 of the cell products formed bone when transplanted in vivo. These validated procedures will serve as the basis for in-house BMSC manufacturing for use in clinical applications in our center.
Jussiani, Eduardo I.; Colaço, Marcos; Kuplish, Leticia; Carrerete, Fabricio; Pinto, R. & Barroso, R Cely [Vis alle 8 forfattere av denne artikkelen] (2018). Mineral composition of urinary stones by single energy desktop microtomography. Journal of Instrumentation (JINST). 13(06). doi: 10.1088/1748-0221/13/06/C06008. Fulltekst i vitenarkiv
Fidalgo, Gabriel; Colaço, Marcos; Nogueira, Liebert Parreiras; Braz, Delson; Silva, Helio & Colaço, Gustavo [Vis alle 7 forfattere av denne artikkelen] (2018). Virtual dissection of Thoropa miliaris tadpole using phase-contrast synchrotron microtomography. Journal of Instrumentation (JINST). 13(5), s. 1–7. doi: 10.1088/1748-0221/13/05/C05012. Fulltekst i vitenarkiv
Kamyar, Negin; Greenhalgh, Ryan; Nascimentoa, Tatiana R. L; Medeiros, Eliton S; Matthews, Peter D & Nogueira, Liebert Parreiras [Vis alle 9 forfattere av denne artikkelen] (2018). Exploiting Inherent Instability of 2D Black Phosphorus for Controlled Phosphate Release from Blow-Spun PLGA Nanofibers. ACS Applied Nano Materials. 1, s. 4190–4197. doi: 10.1021/acsanm.8b00938. Fulltekst i vitenarkiv Vis sammendrag
Efforts have been made to stabilize black phosphorus (BP) to utilize its tunable band gap and anisotropic mechanical properties. Here, the intrinsic instability of BP is exploited for controlled therapeutic ion release, namely phosphate. BP was incorporated into degradable poly (lactide-co-glycolide) fibers via solution blow spinning. Raman spectroscopy confirmed the incorporation of 2D-BP into the nanocomposite along with ICP-AES. It was also demonstrated that modifying the initial loading of 2D-BP in the PLGA fibers permitted tunable release rates of phosphate ions over an 8 weeks in vitro study.
Braz, Delson; Nogueira, Liebert Parreiras; Braz, Delson; Colaço, Marcos; Azambuja, Patricia & Gonzalez, Marcelo S. [Vis alle 10 forfattere av denne artikkelen] (2018). Application of synchrotron radiation phase-contrast microtomography with iodine staining to Rhodnius prolixus head during ecdysis period. Journal of Instrumentation (JINST). 13(05). doi: 10.1088/1748-0221/13/05/C05007. Fulltekst i vitenarkiv

Se alle arbeider i NVA

Boon, Annique van der; Boer, Rosa de; Groot, Bertwin de; Nakrem, Hans Arne; Nogueira, Liebert Parreiras & Ruggiu, Lisa Krämer [Vis alle 8 forfattere av denne artikkelen] (2026). A promising novel recorder of Earth’s ancient magnetic field: fossil micrometeorites as paleomagnetic archives. doi: https:/royalsociety.org/science-events-and-lectures/2026/09/unravelling-magnetic-histories/. Fulltekst i vitenarkiv Vis sammendrag
The paleomagnetic record from sedimentary rocks is often ambiguous, limiting the recovery of reliable paleomagnetic data, particularly when compared to paleomagnetic data obtained from igneous rocks. I will introduce a novel, unexplored recorder of Earth’s magnetic field: fossil micrometeorites. Micrometeorites (MM) are small cosmic particles (50 μm-2mm) that make up a substantial part of the ~40,000 tons of extraterrestrial material delivered to Earth each year. Many MMs melt during atmospheric entry at altitudes of 80-90 km, transforming into cosmic spherules (CS). During this process, particles oxidise and form new minerals, particularly wüstite and magnetite, which record thermoremanent magnetisations upon subsequent cooling. CS are strongly magnetic and can be extracted from sedimentary rocks. Diagnostic features such as metal beads and vesicles inside some CS allow the reconstruction of their flight trajectory, which can be combined with their magnetisations to reconstruct the polarity of Earth’s magnetic field. While modern CS from Antarctica are already known to be robust recorders of the geomagnetic field, their deep-time counterparts remain almost entirely unexplored. I will present the first results of a paleomagnetic study of anthropogenic magnetite spherules that resemble iron-type (I-type) CS, as well as several Paleozoic I-type CS. These 50-300 μm spherules are measurable with standard paleomagnetic tools and, together with flight trajectories obtained using NanoCT scanning, are capable of opening a new window on the ancient geomagnetic field.
Rosenqvist, Marija Plahter; Dunkel, Kristina; Planke, Sverre; Nogueira, Liebert Parreiras; Polteau, Stephane & Menegon, Luca (2025). Porosity structures and local compositional variations associated with natural CO2 sequestration in basalts offshore Norway. doi: 10.5194/egusphere-egu25-11073. Fulltekst i vitenarkiv Vis sammendrag
Permanent storage of CO2 as carbonates in basalt deposits utilises natural weathering reactions between silicate minerals and carbonic water. By studying how CO2-enriched water has altered basalt in 43 samples from the Vøring Margin, offshore Norway, we demonstrate the pathways and reactions of CO2 within a basalt reservoir.The pore spaces occupied by carbonates in the samples were studied through μ-CT (computer tomography) studies on three minicores and detailed microanalyses on 32 thin sections via scanning electron microscopy (SEM), electron microprobe analysis, and electron backscatter diffraction (EBSD). Single crystal X-ray diffraction on 15 samples determined the type of carbonate minerals and precipitation ages were determined for two samples through U/Pb-radiomeric dating. δ18O and δ13C-isotope analyses were used to determine the fluid origin in 11 samples.Three types of carbonate precipitation were characterised based on the pore structures they fill and the associated mineralogy. Type I carbonates, seen in 18 thin sections, fills vesicles, mainly along lava flow margins. SEM analyses showed that all vesicles are initially coated with a smectite layer which incorporates most of the Mg2+ and Fe2+ from dissolving silicate minerals. As pH and Ca concentrations increase, calcite precipitates in some vesicles. The secondary mineral assemblage and precipitation order fit with low-temperature alteration of basalt with pore water and atmospherically balanced CO2 levels (pH≥8). Type II carbonates appear in seven thin sections as
Hildebrand, Torben; Ma, Qianli; Loca, Dagnija; Rubenis, Kristaps; Locs, Janis & Nogueira, Liebert Parreiras [Vis alle 7 forfattere av denne artikkelen] (2025). Contrast-enhanced Micro-CT for improved visualization of ACP-Engineered Osteoblastic Spheroids. Fulltekst i vitenarkiv Vis sammendrag
Introduction: Bone tissue engineering (TE) aims to develop biomaterials that replicate the specific mechanical strength essential for the functional characteristics of the bone. Osteoblastic spheroids provide a three-dimensional (3D) culture model that better mimics in vivo conditions than traditional two-dimensional (2D) cultures. Amorphous calcium phosphate (ACP), derived from eggshell waste, has shown enhanced osteogenic potential, making it a promising biomaterial for TE applications. However, due to limited penetration depth and structural distortion, conventional imaging techniques such as confocal microscopy and histology pose challenges in visualizing 3D constructs. This study evaluates contrast-enhanced micro-computed tomography (micro-CT) as a non-invasive alternative for 3D morphological analysis of ACP-engineered osteoblastic spheroids. Methods: Osteoblastic spheroids were cultivated using flat-bottom, U-bottom, and rotary flask techniques. Spheroids were supplemented with ACP derived from eggshell waste and synthesized ‘control’ ACP to assess its effects on osteogenic differentiation. Contrast-enhanced micro-CT imaging was performed using phosphotungstic acid (PTA) and iodine-based contrast agents. Scans were performed with a laboratory nano-CT (SkyScan 2211 Multiscale x-ray Nano-CT System, Bruker) and at the Synchrotron Radiation for Medical Physics (SYRMEP) beamline in the synchrotron laboratory ELETTRA (Trieste, Italy). Imaging results were compared with confocal laser scanning microscopy, scanning electron microscopy, and classical histology. Results: The cultivation method significantly influenced spheroid morphology, with rotary flask cultivation producing the most structurally uniform spheroids. ACP incorporation enhanced osteogenic activity and spheroid integrity and altered the spheroids’ morphology in volume and roundness. Contrast-enhanced micro-CT provided superior overall visualization of the spheroid and of internal architecture, allowing detailed analysis of cell and ACP distribution and matrix deposition. Unlike traditional imaging techniques, micro-CT enabled virtual histology without sectioning artifacts or depth limitations. Discussion: This study underscores the need for advanced imaging modalities to evaluate engineered tissues effectively. Contrast-enhanced micro-CT offers a non-invasive, high-resolution approach for visualizing cellular interactions within biomaterials, addressing key limitations of conventional imaging techniques. The findings support the integration of ACP into bone TE strategies and demonstrate the potential of micro-CT for enhancing the assessment of biofabricated constructs, particularly for large cell models including a mineralized portion.
Hildebrand, Torben; Lemonche, Gerard Boix; Haugen, Håvard Jostein; Petrovski, Goran & Nogueira, Liebert Parreiras (2024). Contrast-enhanced micro-CT for visualization of cell distribution in hydrated human cornea. Fulltekst i vitenarkiv
Haugen, Håvard Jostein; Nogueira, Liebert Parreiras & Hildebrand, Torben (2023). Novel techniques to visualise cellular tissues using X-ray imaging. Fulltekst i vitenarkiv
Sena, Gabriela; Fidalgo, Gabriel; Paiva, Katrine; Barcellos, Renan; Nogueira, Liebert Parreiras & Colaço, Marcos Vinicius [Vis alle 12 forfattere av denne artikkelen] (2022). Synchrotron X-ray biosample imaging: opportunities and challenges. Biophysical Reviews. ISSN 1867-2450. 14(3), s. 625–633. doi: 10.1007/s12551-022-00964-4. Fulltekst i vitenarkiv
Haugen, Håvard Jostein; Nogueira, Liebert Parreiras & Sunde, Pia Titterud (2021). Use of novel contrast enhanced agent for tissue engineering. Fulltekst i vitenarkiv
Haugen, Håvard Jostein & Nogueira, Liebert Parreiras (2021). Novel contrast agent for visualisation of soft tissue with X-ray imaging. Fulltekst i vitenarkiv
Abadpour, Shadab; Niemi, Essi Maria; Orrhult, Linnea Strid; Nogueira, Liebert Parreiras; Haugen, Håvard Jostein & Josefsen, Dag [Vis alle 10 forfattere av denne artikkelen] (2021). 307.7: 3D Bioprinting of Functional Islets With Adipose-derived Stromal Cells in an Alginate/Nanocellulose Scaffold. Transplantation. ISSN 0041-1337. s. S25–S25. doi: 10.1097/01.tp.0000804424.50335.7e. Fulltekst i vitenarkiv
Qasim, Syed Saad Bin; Nogueira, Liebert Parreiras; Fawzy, Amr S. & Daood, Umer (2020). Correction to: the Effect of Cross-Linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regeneration (AAPS PharmSciTech, (2020), 21, 5, (173), 10.1208/s12249-020-01708-x). AAPS PharmSciTech. 21(7). doi: 10.1208/s12249-020-01778-x. Fulltekst i vitenarkiv

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Publisert 3. jan. 2018 10:36 - Sist endret 15. sep. 2023 14:08

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DIRE – Data Visualization Remote Access