Siste publikasjoner

• Vilaça, Adriana; Domingues, Rui M. A,; Tiainen, Hanna; Mendes, Bárbara B.; Barrantes Bautista, Alejandro; Reis, Rui L.; Gomes, Manuela E. & Gomez-Florit, Manuel (2021). Multifunctional surfaces for improving soft tissue integration. Advanced Healthcare Materials.  ISSN 2192-2640. . doi: 10.1002/adhm.202001985
• Skudutyte-Rysstad, Rasa; Sunde, Pia Titterud; Sen, Abhijit & Jordal, Kristin (2021). Effects of an individualised training course in endodontics on the knowledge and insights of dentists in Public Dental Service in Norway. Acta Odontologica Scandinavica.  ISSN 0001-6357. . doi: 10.1080/00016357.2021.1876915
• Enriquez, J. J. S.; Sena, Gabriela; Braz, Delson; Barroso, Regina C.; Colaço, Marcos; Fidalgo, Gabriel; Nogueira, Liebert Parreiras; Azambuja, Patricia & Gonzalez, Marcelo S. (2021). The Methodology to produce Rhodnius prolixus head structures made by 3D printing using Synchrotron Radiation Microtomography. Journal of Instrumentation (JINST).  ISSN 1748-0221. . doi: 10.1088/1748-0221/16/02/P02014
• Monsen, Ragnhild Elisabeth; Herlofson, Bente Brokstad; Gay, Caryl; Fjeld, Katrine Gahre; Hove, Lene Hystad; Malterud, Karl Egil; Saghaug, Elisabeth; Slaaen, Joran; Sundal, Tone; Tollisen, Anita & Lerdal, Anners (2021). A mouth rinse based on a tea solution of Salvia officinalis for oral discomfort in palliative cancer care: A randomized controlled trial. Supportive Care in Cancer.  ISSN 0941-4355. . doi: 10.1007/s00520-021-06021-2 Fulltekst i vitenarkiv. Vis sammendrag

  Background: Few clinical studies evaluate interventions to reduce oral discomfort among patients in palliative care. Aim: This study examines the efficacy of a Salvia officinalis (SO) based herbal mouth rinse compared to conventional normal saline (NS) in order to improve oral health. Design: A block-randomized controlled trial. Data were collected before and after a 4-day intervention with either SO (n=44) or NS (n=44). Numerical rating scales (NRS, 0-10) and 12 items from the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire-Oral Health 17 (EORTC QLQ-OH17) measured patient-reported oral symptoms. An oral examination was performed before and after the intervention. Setting/participants: This study included adult patients with late-stage cancer in an inpatient hospice unit. Results: Of the 88 patients included (mean age=63.9 years, SD=10.6), 73 (83%) completed the study. At baseline, 78% reported dry mouth on the EORTC QLQ-OH17, and 80% rated dry mouth ≥4 on the NRS. Total oral health scores based on the 12 EORTC QLQ-OH17 items improved similarly in both groups (p

• Gerkin, Richard C; Ohla, Kathrin; Veldhuizen, Marie G; Joseph, Paule V; Kelly, Christine E; Bakke, Alyssa J; Singh, Preet Bano; Ahuja, Gaurav; Hummel, Thomas; Reed, Danielle R; Niv, Masha Y; Munger, Steven D & Parma, Valentina (2021). Recent smell loss is the best predictor of COVID-19 among individuals with recent respiratory symptoms.. Chemical Senses.  ISSN 0379-864X. . doi: https://doi.org/10.1093/chemse/bjaa081
• Yang, Yang; Pullisaar, Helen; Landin, Maria Augusta Dos S Silva; Heyward, Catherine Anne; Schröder, Maria; Geng, Tianxiang; Grano, Maria & Reseland, Janne Elin (2021). FNDC5/irisin is expressed and regulated differently in human periodontal ligament cells, dental pulp stem cells and osteoblasts. Archives of Oral Biology.  ISSN 0003-9969. . doi: https://doi.org/10.1016/j.archoralbio.2021.105061 Fulltekst i vitenarkiv.
• Magaz, Andrew; Li, X; Gough, Julie e. & Blaker, Jonathan James (2021). Graphene oxide and electroactive reduced graphene oxide/silk composite fibrous scaffolds for engineering excitable nerve tissue. Materials Science and Engineering C: Materials for Biological Applications.  ISSN 0928-4931.  119 . doi: 1016/j.msec.2020.111632
• Papageorgiou, Spyridon; Tilen, Raphael; Vandevska-Radunovic, Vaska & Eliades, Theodore (2021). Occlusal outcome after orthodontic treatment with preadjusted straight-wire and standard edgewise appliances A retrospective cohort study. Journal of Orofacial Orthopedics.  ISSN 1434-5293. . doi: https://doi.org/10.1007/s00056-020-00273-z Fulltekst i vitenarkiv.
• Thieu, Minh Khai Le; Haugen, Håvard Jostein; Esporrin, Javier Sanz; Sanz, Mariano; Lyngstadaas, Ståle Petter & Verket, Anders (2021). Guided bone regeneration of chronic non-contained bone defects using a volume stable porous block TiO2 scaffold: An experimental in vivo study. Clinical Oral Implants Research.  ISSN 0905-7161. . doi: 10.1111/clr.13708 Vis sammendrag

  Objectives To evaluate new lateral bone formation and lateral volume augmentation by guided bone regeneration (GBR) in chronic non‐contained bone defects with the use of a non‐resorbable TiO2‐block. Materials and methods Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed to heal for eight weeks before treatment by GBR. Each hemimandible was randomly allocated to 4 or 12‐week healing time after GBR and three intervention groups were assigned by block randomization: TiO2 block: TiO2‐scaffold and a collagen membrane, DBBM particles: Deproteinized bovine bone mineral (DBBM) and a collagen membrane, Empty control: Collagen membrane only. Microcomputed tomography (microCT) was used to measure the lateral bone formation and width augmentation. Histological outcomes included descriptive analysis and histomorphometric measurements. Results MicroCT analysis demonstrated increasing new bone formation from four to twelve weeks of healing. The greatest width of mineralized bone was seen in the empty controls, and the largest lateral volume augmentation was observed in the TiO2 block sites. The DBBM particles demonstrated more mineralized bone in the grafted area than the TiO2 blocks, but small amounts and less than the empty control sites. Conclusion The TiO2 blocks rendered the largest lateral volume augmentation but also less new bone formation compared to the DBBM particles. The most new lateral bone formation outward from the bone defect margins was observed in the empty controls, indicating that the presence of either graft material leads to slow appositional bone growth.

• Di Raimondo, Ricardo; Sanz, Javier; Sanz Martin1, Ignacio; Vignoletti, Fabio; Nuñez, Javi; Muñoz, Fernando; Haugen, Håvard Jostein & Sanz, Mariano (2021). Hard tissue volumetric and soft tissue contour linear changes at implants with different surface characteristics after experimentally induced peri-implantitis: an experimental in vivo investigation. Clinical Oral Investigations.  ISSN 1432-6981. . doi: 10.1007/s00784-020-03720-8 Vis sammendrag

  Objective To evaluate the hard tissue volumetric and soft tissue contour linear changes in implants with two different implant surface characteristics after a ligature-induced peri-implantitis. Material andmethods In eight beagle dogs, implants with the same size and diameter but distinct surface characteristics were placed in the healed mandibular sites. Test implants had an external monolayer of multi-phosphonate molecules (B+), while control implants were identical but without the phosphonate-rich surface. Once the implants were osseointegrated, oral hygiene was interrupted and peri-implantitis was induced by placing subgingival ligatures. After 16 weeks, the ligatures were removed and peri-implantitis progressed spontaneously. Bone to implant contact (BIC) and bone loss (BL) were assessed three-dimensionally with Micro-Ct (μCT). Dental casts were optically scanned and the obtained digitalized standard tessellation language (STL) images were used to assess the soft tissue vertical and horizontal contour linear changes. Results Reduction of the three-dimensional BIC percentage during the induction and progression phases of the experimental peri-implantitis was similar for both the experimental and control implants, without statistically significant differences between them. Soft tissue analysis revealed for both implant groups an increase in horizontal dimension after the induction of peri-implantitis, followed by a decrease after the spontaneous progression period. In the vertical dimension, a soft tissue dehiscence was observed in both groups, being more pronounced at the buccal aspect. Conclusions The added phosphonate-rich surface did not provide a more resistant environment against experimental periimplantitis, when assessed by the changes in bone volume and soft tissue contours. Clinical relevance Ligature-induced peri-implantitis is a validated model to study the tissue changes occurring during peri-implantitis. It was hypothesized that a stronger osseointegration mediated by the chemical bond of a phosphonaterich implant surface would develop an environment more resistant to the inflammatory changes occurring after experimental peri-implantitis. These results, however, indicate that the hard and soft tissue destructive changes occurring at both the induction and progression phases of experimental peri-implantitis were not influenced by the quality of osseointegration.

• Sena, Gabriela; Barroso, Regina C.; Braz, Delson; Nogueira, Liebert Parreiras; Colaço, Marcos; Kourousias, George; Tromba, Giuliana; Azambuja, Patricia; Gonzales, Marcelo; Pickler, Arissa; Fidalgo, Gabriel; Paiva, Katrine & Barcellos, Renan (2021). Evaluation of the effects of Azadirachtin on internal structures of Rhodnius prolixus head using low-energy X-ray microfluorescence. Spectrochimica Acta Part B - Atomic Spectroscopy.  ISSN 0584-8547. . doi: 10.1016/j.sab.2020.106064
• Colaço, Marcos; Camara, Alexandre Resende; Nogueira, Liebert Parreiras; Carvalho, Isabel C. S.; Margulis, Walter & Barroso, Regina C. (2021). Characterization of microstructures of specialty optical fibers for electric field sensing by propagation-based X-ray phase-contract tomography. Measurement Science and Technology.  ISSN 0957-0233. . doi: 10.1088/1361-6501/abd365 Fulltekst i vitenarkiv.
• Vyas, Cian; Zhang, Jun; Øvrebø, Øystein; Huang, Boyang; Roberts, Iwan; Setty, Mohan; Allardyce, Benjamin J; Haugen, Håvard Jostein; Rajkhowa, Rangam & Bàrtolo, Paulo Jorge da Silva (2021). 3D printing of silk microparticle reinforced polycaprolactone scaffolds for tissue engineering applications. Materials Science and Engineering C: Materials for Biological Applications.  ISSN 0928-4931.  118, s 111433 . doi: 10.1016/j.msec.2020.111433 Fulltekst i vitenarkiv. Vis sammendrag

  Polycaprolactone (PCL) scaffolds have been widely investigated for tissue engineering applications, however, they exhibit poor cell adhesion and mechanical properties. Subsequently, PCL composites have been produced to improve the material properties. This study utilises a natural material, Bombyx mori silk microparticles (SMP) prepared by milling silk fibre, to produce a composite to enhance the scaffolds properties. Silk is biocompatible and biodegradable with excellent mechanical properties. However, there are no studies using SMPs as a reinforcing agent in a 3D printed thermoplastic polymer scaffold. PCL/SMP (10, 20, 30 wt. %) composites were prepared by melt blending. Rheological analysis showed that SMP loading increased the shear thinning and storage modulus of the material. Scaffolds were fabricated using a screw-assisted extrusion-based additive manufacturing system. Scanning electron microscopy and x-ray microtomography was used to determine scaffold morphology. The scaffolds had high interconnectivity with regular printed fibres and pore morphologies within the designed parameters. Compressive mechanical testing showed that the addition of SMP significantly improved the compressive Young’s modulus of the scaffolds. The scaffolds were more hydrophobic with the inclusion of SMP which was linked to a decrease in total protein adsorption. Cell behaviour was assessed using human adipose derived mesenchymal stem cells. A cytotoxic effect was observed at higher particle loading (30 wt. %) after 7 days of culture. By day 21, 10 wt. % loading showed significantly higher cell metabolic activity and proliferation, high cell viability, and cell migration throughout the scaffold. Calcium mineral deposition was observed on the scaffolds during cell culture. Large calcium mineral deposits were observed at 30 wt. % and smaller calcium deposits were observed at 10 wt. %. This study demonstrates that SMPs incorporated into a PCL scaffold provided effective mechanical reinforcement, improved the rate of degradation, and increased cell proliferation, demonstrating potential suitability for bone tissue engineering applications.

• Sukul, Mousumi; Sahariah, Priyanka; Lauzon, Hélène L.; Borges, João; Másson, Már; Mano, Joao F.; Haugen, Håvard Jostein & Reseland, Janne Elin (2021). In vitro biological response of human osteoblasts in 3D chitosan sponges with controlled degree of deacetylation and molecular weight. Carbohydrate Polymers.  ISSN 0144-8617.  254, s 117434 . doi: 10.1016/j.carbpol.2020.117434 Fulltekst i vitenarkiv. Vis sammendrag

  We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw. In summary, the variations in cellular responses to the different chitosan sponges indicate a potential for individual tailoring of desired responses in guided

• Chvartszaid, David; Ellingsen, Jan Eirik; Estafanous, Emad; Huynh-Ba, Guy; Oates, Thomas; Osswald, Martin & Stanford, Clark (2020). Dental student implant education. International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(6), s 1072- 1075
• Osswald, Martin; Chvartszaid, David; Ellingsen, Jan Eirik; Estafanous, Emad; Huynh-Ba, Guy; Oates, Thomas & Stanford, Clark (2020). "The lucky fibula 2.0". International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(5), s 875- 877
• Tellefsen, Sara Julie Maria; Badian, Reza; Utheim, Tor Paaske; Utheim, Øygunn Aass; Stojanovic, Aleksandar; Tashbayev, Behzod; Ræder, Sten; Dartt, Darlene Ann & Chen, Xiangjun (2020). Sex and age differences in symptoms and signs of dry eye disease in a Norwegian cohort of patients. The ocular surface.  ISSN 1542-0124.  19, s 68- 73 . doi: 10.1016/j.jtos.2020.11.009
• Wiechmann, Dirk; Bantleon, Hans-Peter; Melsen, Birte; Zachrisson, Bjørn Uddo; Hägg, Urban; Canal, Pierre; Garcia, Robert; Barthélemi, Stephane; Frapier, Laure; Grauer, Dan; Sander, Christian; Diedrich, Peter; Jacobs, Collin; Wehrbein, Heiner; Hohoff, Ariane; Helms, Hans-Joachim & Schwestka-Polly, Rainer (2020). Incorrect measurements and misleading conclusions in the article "comparison of the efficacy of tooth alignment among lingual and labial brackets: An in vitro study". Head & Face Medicine.  ISSN 1746-160X.  16(1) . doi: 10.1186/s13005-020-00221-7
• Staxrud, Frode (2020). Minimally Invasive Dentistry. Aktuell Nordisk odontologi.  ISSN 1902-3545.  45(01), s 225- 238 . doi: 10.18261/ISSN.2058-7538-2020-01-18 Vis sammendrag

  Begrepet «Minimally Invasive Dentistry» er etter hvert godt kjent, både i Norden og Europa for øvrig. Det er allikevel viktig å minne om oss selv om de grunnleggende prinsippene i dette konseptet. Med bakgrunn i oppdatert kunnskap om karies og tannslitasje, kombinert med nyere og bedre adhesive restaureringsteknikker, er det gode muligheter for å behandle tenner på en vevsbesparende måte slik at de holder livet ut. Pasienten må settes i fokus slik at den totale behandling individualiseres. Med kjennskap til pasienten og den enkeltes særlige vilkår kan vi skreddersy en plan for hver enkelt. Forebyggelse og kontroll på risikofaktorer forut for operativ behandling og en konservativ innstilling til original tannsubstans er gode grunnleggende prinsipper.

• Halvari, Anne Elisabeth M.; Halvari, Halgeir & Deci, Edward (2020). The roles of patients' authenticity and accepting external influence, and clinicians' treatment styles in predicting patients' dental anxiety and avoidance of dental appointments. Europe's Journal of Psychology.  ISSN 1841-0413.  16(1), s 45- 61 . doi: 10.5964/ejop.v16i1.1664 Fulltekst i vitenarkiv. Vis sammendrag

  A substantial proportion of adults suffer from high dental anxiety, which is related to poor oral health and functioning. Using authenticity theory and self-determination theory, we applied a model testing two moderated mediation hypotheses: (i) the negative indirect association between authenticity and avoiding dental appointments through dental anxiety would be more evident when clinicians provides higher levels of autonomy support; and (ii) the indirect positive association between accepting external influence and avoiding dental appointments through dental anxiety would be more evident when clinicians provides higher levels of controllingness. Participants (N = 208) responded to a survey with validated questionnaires. The model with hypotheses were tested using Structural Equation Modeling (SEM) in LISREL and Conditional Process Modeling (moderated mediation). The results supported our hypotheses. The SEM model tested was found to fit the data well. Patient’s personality and dental clinic treatment environments predicted 38% of the variance in dental anxiety, which explained 38% of avoidance of treatment.

• Zou, Lai-quan; Dworschak, Annika; Alizadeh, Rafieh; Kamrawa, Seyed; Alwashahi, Mohammed; Bock, Marie; Boesveldt, Sanne; Singh, Preet Bano; Brusevold, Ingvild Johnsen; Voznessenskaya, Vera; Hummel, Thomas & Schriever, Valentin A (2020). “U-Sniff ” - the international odor identification test for children: an extension of its normative database and study of global reliability.. Rhinology.  ISSN 0300-0729.  45(7) . doi: 10.4193/Rhin19.355
• Tashbayev, Behzod; Yazdani, Mazyar; Arita, Reiko; Fineide, Fredrik & Utheim, Tor Paaske (2020). Intense pulsed light treatment in meibomian gland dysfunction: A concise review. The ocular surface.  ISSN 1542-0124.  18(4), s 583- 594 . doi: 10.1016/j.jtos.2020.06.002
• Varvin, Therese; Søftestad, Siri; Kranstad, Vibeke & Willumsen, Tiril (2020). Preparing for attack and recovering from battle: Understanding child sexual abuse survivors' experiences of dental treatment. Community Dentistry and Oral Epidemiology.  ISSN 0301-5661.  48(4), s 317- 327 . doi: 10.1111/cdoe.12536
• Magaz, Andrew; Spencer, Ben F.; Hardy, John G.; Xu, Li; Gough, Julie e. & Blaker, Jonathan James (2020). Modulation of Neuronal Cell Affinity on PEDOT−PSS Nonwoven Silk Scaffolds for Neural Tissue Engineering. ACS Biomaterials Science and Engineering.  ISSN 2373-9878. . doi: 10.1021/acsbiomaterials.0c01239 Vis sammendrag

  Peripheral nerve injury is a common consequence of trauma with low regenerative potential. Electroconductive scaffolds can provide appropriate cell growth microenvironments and synergistic cell guidance cues for nerve tissue engineering. In the present study, electrically conductive scaffolds were prepared by conjugating poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT–PSS) or dimethyl sulfoxide (DMSO)-treated PEDOT–PSS on electrospun silk scaffolds. Conductance could be tuned by the coating concentration and was further boosted by DMSO treatment. Analogue NG108-15 neuronal cells were cultured on the scaffolds to evaluate neuronal cell growth, proliferation, and differentiation. Cellular viability was maintained on all scaffold groups while showing comparatively better metabolic activity and proliferation than neat silk. DMSO-treated PEDOT–PSS functionalized scaffolds partially outperformed their PEDOT–PSS counterparts. Differentiation assessments suggested that these PEDOT–PSS assembled silk scaffolds could support neurite sprouting, indicating that they show promise to be used as a future platform to restore electrochemical coupling at the site of injury and preserve normal nerve function.

• Rahmati, Maryam; Mills, David K.; Urbanska, Aleksandra M.; Saeb, Mohammad reza; Reddy Venugopal, Jayarama; RamaKrishna, Seeram & Mozafari, Masoud (2020). Electrospinning for tissue engineering applications. Progress in Materials Science.  ISSN 0079-6425.  s 1- 39 . doi: 10.1016/j.pmatsci.2020.100721 Vis sammendrag

  Tissue engineering makes use of the principles of medicine, biology and engineering and integrates them into the design of biological substitutes to restore, maintain and improve the functions of tissue. To fabricate a functional tissue, the engineered structures have to be able to mimic the extracellular matrix (ECM), provide the tissue with oxygen and nutrient circulation as well as remove metabolic wastes in the period of tissue regeneration. Continued efforts have been made in order to fabricate advanced functional three-dimensional scaffolds for tissue engineering. Electrospinning has been recognized and served as one of the most useful techniques based on the resemblance between electrospun fibers and the native tissues. Over the past few decades, a bewildering variety of nanofibrous scaffolds have been developed for various biomedical applications, such as tissue regeneration and therapeutic agent delivery. The present review aims to provide with researchers an in-depth understanding of the promising role and the practical region of applicability of electrospinning in tissue engineering and regenerative medicine by highlighting the outcomes of the most recent studies performed in this field. We address the current strategies used for improving the physicochemical interactions between the cells and the nanofibrous surface. We also discuss the progress and challenges associated with the use of electrospinning for tissue engineering and regenerative medicine applications.

• Preus, Hans Ragnar; Al-Lami, amar Saad Abdulraheem & Baelum, Vibeke (2020). Oral hygiene revisited. The clinical effect of a prolonged oral hygiene phase prior to periodontal therapy in periodontitis patients. A randomized clinical study. Journal of Clinical Periodontology.  ISSN 0303-6979.  47(1), s 36- 42 . doi: 10.1111/jcpe.13207
• Kapralos, Vasileios; Valen, Håkon; Ørstavik, Dag; Koutroulis, Andreas; Camilleri, Josette & Sunde, Pia Titterud (2020). Antimicrobial and physicochemical characterization of endodontic sealers after exposure to chlorhexidine digluconate. Dental Materials.  ISSN 0109-5641. . doi: 10.1016/j.dental.2020.11.011
• Mauland, Erik Klepsland; Preus, Hans Ragnar & Aass, Anne Merete (2020). Comparison of commercially available 0.2% chlorhexidine mouthwash with and without anti-discoloration system: A blinded, crossover clinical trial. Journal of Clinical Periodontology.  ISSN 0303-6979.  47(12), s 1522- 1527 . doi: doi.org/10.1111/jcpe.13382 Fulltekst i vitenarkiv.
• Rizk, Mumen Z.; Torgersbråten, Nina Ellen; Mohammed, Hisham; Franzen, Tanya Jeanette & Vandevska-Radunovic, Vaska (2020). Stability of single-jaw vs two-jaw surgery following the correction of skeletal class III malocclusion: A systematic review and meta-analysis. Orthodontics & craniofacial research.  ISSN 1601-6335. . doi: DOI: 10.1111/ocr.12456
• Parma, Valentina; Ohla, Kathrin; Veldhuizen, MK; Niv, Masha Y.; Kelly, Christine E.; Bakke, Alyssa J.; Cooper, Keiland W.; Bouysset, Cedric; Pirastu, Nicola; Dibattista, Michele; Kaur, Rishemjit; Liuzza, Marco Tullio; Pepino, Marta Y.; Schöpf, Veronika; Pereda-Loth, Veronica; Olsson, Shannon B.; Gerkin, Richard C.; Dominguez, Paloma Rohlfs; Albayay, Javier; Farruggia, Michael C.; Bhutani, Surabhi; Fjaeldstad, Alexander W.; Kumar, Ritesh; Menini, Anna; Bensafi, Moustafa; Sandell, Mari; Konstantinidis, Iordanis; Di Pizio, Antonella; Genovese, Federica; Ôztürk, Lina; Thomas-Danguin, Thierry; Frasnelli, Johannes; Boesveldt, Sanne; Saatci, Ôzlem; Saraiva, Luis R.; Lin, Cailu; Golebiowski, Jerome; Hwang, Liang-Dar; Ozdener, Mehmet Hakan; Guàrdia, Maria Dolors; Laudamiel, Christophe; Ritchie, Marina; Havlicek, Jan; Pierron, Denis; Roura, Eugeni; Navarro, Marta; Nolden, Alissa A.; Lim, Juyun; Whitcroft, Katherine L.; Colquitt, Lauren R.; Singh, Preet Bano & Hayes, John E. (2020). More than smell- Covid-19 is associated with severe impairment of smell, taste, and chemethesis.. Chemical Senses.  ISSN 0379-864X.  45(7), s 609- 622 . doi: https://doi.org/10.1093/chemse/bjaa041
• Grytten, Jostein Ivar; Pervaiz, Maryam; Rongen, Gunnar & Skau, Irene (2020). De unge voksnes tannhelse - hvor stort er deres behandlingsbehov? En analyse av studenter som var pasienter hos SiO Helse Tannlege i perioden 2007-2018. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130, s 904- 912
• Fardal, Øystein; Skau, Irene; Rongen, Gunnar; Heasman, Peter & Grytten, Jostein Ivar (2020). Treatment provision of periodontal diseases in Norway in 2013 - a national profile.. International Dental Journal.  ISSN 0020-6539.  70(4), s 266- 276 . doi: https://doi.org/10.1111/idj.12565 Fulltekst i vitenarkiv.
• Hvaring, Christina & Birkeland, Kari (2020). The long-term fate of persisting deciduous molars and canines in 42 patients with severe hypodontia: a 12-year follow-up. European Journal of Orthodontics.  ISSN 0141-5387.  42(6), s 581- 586 . doi: https://doi.org/10.1093/ejo/cjz090
• Hamran, Tord & Vandevska-Radunovic, Vaska (2020). Bruk av skinner innen tannregulering. Aktuell Nordisk odontologi.  ISSN 1902-3545.  45, s 153- 165 . doi: DOI: HTTPS://DOI.ORG/10.18261/ISSN.2058-7538-2020-01-13
• Bjering, Ragnar & Vandevska-Radunovic, Vaska (2020). Hvor stabil er kjeveortopedisk behandling på sikt, og hvilken betydning har retainere?. Aktuell Nordisk odontologi.  ISSN 1902-3545.  45, s 166- 178 . doi: DOI: HTTPS://DOI.ORG/10.18261/ISSN.2058-7538-2020-01-14
• Hugo, Jørgen; Koldsland, Odd Carsten; Aass, Anne Merete & Tiainen, Hanna (2020). Development and initial testing of an in vitro model simulating class II furcation defects. Clinical and Experimental Dental Research.  ISSN 2057-4347. . doi: 10.1002/cre2.346 Fulltekst i vitenarkiv.
• Koldsland, Odd Carsten & Aass, Anne Merete (2020). Supportive treatment following peri-implantitis surgery: An RCT using titanium curettes or chitosan brushes. Journal of Clinical Periodontology.  ISSN 0303-6979.  47(10), s 1259- 1267 . doi: 10.1111/jcpe.13357
• Vetyskova, Veronika; Zouharova, Monika; Bednarova, Lucie; Vaněk, Ondřej; Sázelová, Petra; Kašička, Václav; Vymetal, Jiri; Srp, Jaroslav; Rumlová, Michaela; Charnavets, Tatsiana; Postulkova, Klara; Reseland, Janne Elin; Bousova, Kristyna & Vondrasek, Jiri (2020). Characterization of AMBN I and II isoforms and study of their Ca2+ binding properties. International Journal of Molecular Sciences.  ISSN 1422-0067.  21, s 9293 . doi: 10.3390/ijms21239293 Fulltekst i vitenarkiv.
• Magaz, Adrián; Spencer, Ben F.; Hardy, John G.; Li, Xu; Gough, Julie E. & Blaker, Jonathan James (2020). Modulation of Neuronal Cell Affinity on PEDOT-PSS Nonwoven Silk Scaffolds for Neural Tissue Engineering. ACS Biomaterials Science and Engineering.  ISSN 2373-9878. . doi: 10.1021/acsbiomaterials.0c01239
• Zhu, Hao; Blahnová, Veronika Hefka; Perale, Giuseppe; Xiao, Jun; Betge, Felice; Boniolo, Fabio; Filová, Eva; Lyngstadaas, Ståle Petter & Haugen, Håvard Jostein (2020). Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs. Frontiers in Cell and Developmental Biology.  ISSN 2296-634X. . doi: 10.3389/fcell.2020.619111
• Tashbayev, Behzod; Utheim, Tor Paaske; Utheim, Øygunn Aass; Ræder, Sten; Jensen, Janicke Cecilie Liaaen; Yazdani, Mazyar; Lagali, Neil Satish; Vitelli, Valeria; Dartt, Darlene Ann & Chen, Xiangjun (2020). Utility of Tear Osmolarity Measurement in Diagnosis of Dry Eye Disease. Scientific Reports.  ISSN 2045-2322.  s 1- 7 . doi: 10.1038/s41598-020-62583-x Fulltekst i vitenarkiv.
• Brennhovd, Anders; Petrovski, Beata & Hjortsjö, Carl (2020). Benoppbygging før implantatinnsetting – overlevelse og suksess. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130(10), s 796- 805
• Seitz, Elke; Hjortsjö, Carl; Dahl, Jon Einar & Saxegaard, Erik (2020). Dentin to dentin adhesion using combinations of resin cements and adhesives from different manufacturers - a novel approach. Biomaterial Investigations in Dentistry.  7(1), s 96- 104 . doi: 10.1080/26415275.2020.1793677 Fulltekst i vitenarkiv.
• Thorlacius, Gudny Ella; Hultin-Rosenberg, Lina; Sandling, Johanna K.; Bianchi, Matteo; Imgenberg-Kreuz, Juliana; Pucholt, Pascal; Theander, Elke; Kvarnström, Marika; Forsblad-d’Elia, Helena; Bucher, Sara Magnusson; Norheim, Katrine Brække; Johnsen, Svein Joar Auglænd; Hammenfors, Sten Daniel; Skarstein, Kathrine; Jonsson, Malin V; Bäcklund, Eva; Aqrawi, Lara A.; Jensen, Janicke Liaaen; Palm, Øyvind; Morris, Andrew P.; consortium, the DISSECT; meadows, jennifer r; Rantapää-Dahlqvist, Solbritt; Mandl, Thomas; Eriksson, Per; Lind, Lars; Omdal, Roald; Jonsson, Sten Ture Roland; Lindblad-Toh, Kerstin; Rönnblom, Lars; Wahren-Herlenius, Marie & Nordmark, Gunnel (2020). Genetic and clinical basis for two distinct subtypes of primary Sjögren's syndrome. Rheumatology.  ISSN 1462-0324. . doi: 10.1093/rheumatology/keaa367
• Weber, Florian; Sagstuen, Einar; Zhong, Qi-Zhi; Zheng, Tian & Tiainen, Hanna (2020). Tannic Acid Radicals in the Presence of Alkali Metal Salts and Their Impact on the Formation of Silicate-Phenolic Networks. ACS Applied Materials & Interfaces.  ISSN 1944-8244.  12(47), s 52457- 52466 . doi: 10.1021/acsami.0c16946 Fulltekst i vitenarkiv.
• Kallas, Pawel; Haugen, Håvard Jostein; Gadegaard, Nikolaj; Stormonth-Darling, John; Hulander, Mats; Andersson, Martin & Valen, Håkon (2020). Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae. Nanomaterials.  ISSN 2079-4991.  10(11), s 2247 . doi: 10.3390/nano10112247 Fulltekst i vitenarkiv. Vis sammendrag

  Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.

• Øyri, Hauk Furnes; Jensen, Janicke Liaaen; Barkvoll, Pål; Jonsdottir, Olga Hrønn; Reseland, Janne Elin & Bjørnland, Tore (2020). Incidence of alveolar osteitis after mandibular third molar surgery. Can inflammatory cytokines be identified locally?. Acta Odontologica Scandinavica.  ISSN 0001-6357.  s 1- 7 Fulltekst i vitenarkiv.
• Fidalgo, Gabriel; Paiva, Katrine; Mendes, Gabriela; Barcellos, Renan; Colaço, Gustavo; Sena, Gabriela; Pickler, Arissa; Mota, Carla Lemos; Tromba, Giuliana; Nogueira, Liebert Parreiras; Braz, Delson; Silva, H; Colaço, Marcos & Barroso, Regina C. (2020). Synchrotron microtomography applied to the volumetric analysis of internal structures of Thoropa miliaris tadpoles. Scientific Reports.  ISSN 2045-2322.  10 . doi: 10.1038/s41598-020-75993-8 Fulltekst i vitenarkiv. Vis sammendrag

  Amphibians are models for studying applied ecological issues such as habitat loss, pollution, disease, and global climate change due to their sensitivity and vulnerability to changes in the environment. Developmental series of amphibians are informative about their biology, and X-ray based 3D reconstruction holds promise for quantifying morphological changes during growth—some with a direct impact on the possibility of an experimental investigation on several of the ecological topics listed above. However, 3D resolution and discrimination of their soft tissues have been difficult with traditional X-ray computed tomography, without time-consuming contrast staining. Tomographic data were initially performed (pre-processing and reconstruction) using the open-source software tool SYRMEP Tomo Project. Data processing and analysis of the reconstructed tomography volumes were conducted using the segmentation semi-automatic settings of the software Avizo Fire 8, which provide information about each investigated tissues, organs or bone elements. Hence, volumetric analyses were carried out to quantify the development of structures in different tadpole developmental stages. Our work shows that synchrotron X-ray microtomography using phase-contrast mode resolves the edges of the internal tissues (as well as overall tadpole morphology), facilitating the segmentation of the investigated tissues. Reconstruction algorithms and segmentation software played an important role in the qualitative and quantitative analysis of each target structure of the Thoropa miliaris tadpole at different stages of development, providing information on volume, shape and length. The use of the synchrotron X-ray microtomography setup of the SYRMEP beamline of Elettra Synchrotron, in phase-contrast mode, allows access to volumetric data for bone formation, eye development, nervous system and notochordal changes during the development (ontogeny) of tadpoles of a cycloramphid frog Thoropa miliaris. As key elements in the normal development of these and any other frog tadpole, the application of such a comparative ontogenetic study, may hold interest to researchers in experimental and environmental disciplines.

• Nigi, Laura; Brusco, Noemi; Grieco, Giuseppina E.; Licata, Giada; Krogvold, Lars; Marselli, Lorella; Gysemans, Conny; Overbergh, Lut; Marchetti, Piero; Mathieu, Chantal; Dahl-Jørgensen, Knut; Sebastiani, Guido & Dotta, Francesco (2020). Pancreatic alpha-cells contribute together with beta-cells to CXCL10 expression in type 1 diabetes. Frontiers in Endocrinology.  ISSN 1664-2392.  11:630, s 1- 13 . doi: 10.3389/fendo.2020.00630
• Hesse, Juliane; Schmalfuss, Andreas Jörn & Kvaal, Sigrid Ingeborg (2020). Photodynamic therapy of oral lichen planus. Photochemical and Photobiological Sciences.  ISSN 1474-905X.  19, s 1271- 1279 . doi: 10.1039/d0pp00249f
• Gross, Karlis Agris; Petzold, Christiane; Pluduma, Liene; Kumermanis, Maris & Haugen, Håvard Jostein (2020). Structural and Chemical Hierarchy in Hydroxyapatite Coatings. Materials.  ISSN 1996-1944.  13(4447), s 1- 15 Fulltekst i vitenarkiv. Vis sammendrag

  Hydroxyapatite coatings need similarly shaped splats as building blocks and then a homogeneous microstructure to unravel the structural and chemical hierarchy for more refined improvements to implant surfaces. Coatings were thermally sprayed with differently sized powders (20-40, 40-63 and 63-80 µm) to produce flattened homogeneous splats. The surface was characterized for splat shape by profilometry and AFM, crystal size by AFM, crystal orientation by X-ray diffraction (XRD) and structural variations by XRD. Chemical composition was assessed by phase analysis, but variations in chemistry were detected by XRD and Raman spectroscopy. The resulting surface electrical potential was measured by Kelvin probe AFM. Five levels of structural hierarchy were suggested: the coating, the splat, oriented crystals, alternate layers of oxyapatite and HAp, and the suggested anion orientation. Chemical hierarchy was present over a lower range of order for smaller splats. Coatings made from smaller splats exhibited a greater electrical potential, inferred to arise from oxyapatite, and supplemented by ordered OH- ions in a rehydroxylated surface layer. A model has been proposed to show the influence of structural hierarchy on the electrical surface potential. Structural hierarchy is proposed as a means to further refine the properties of implant surfaces.

• Tulek, Amela; Mulic, Aida; Stenhagen, Kjersti Refsholt; Galtung, Hilde; Saeed, Muhammad; Utheim, Tor Paaske; Khuu, Cuong; Galteland, Pål & Sehic, Amer (2020). Dental Erosion in Mice with Impaired Salivary Gland Function. Acta Odontologica Scandinavica.  ISSN 0001-6357.  78, s 1- 11 . doi: 10.1080/00016357.2020.1734234
• Aqrawi, Lara A.; Chen, Xiangjun; Hynne, Håvard; Amdal, Cecilie Delphin; Reppe, Sjur; Aass, Hans Christian Dalsbotten; Rykke, Morten; Hove, Lene Hystad; Young, Alix; Herlofson, Bente Brokstad; Westgaard, Kristine Løken; Utheim, Tor Paaske; Galtung, Hilde & Jensen, Janicke Liaaen (2020). Cytokines Explored in Saliva and Tears from Radiated Cancer Patients Correlate with Clinical Manifestations, Influencing Important Immunoregulatory Cellular Pathways. Cells.  ISSN 2073-4409.  9(9) . doi: 10.3390/cells9092050 Fulltekst i vitenarkiv.
• Bunæs, Dagmar; Verket, Anders; Aass, Anne Merete & Bolstad, Anne Isine (2020). Tyypin 2 diabeteksen ja parodontaalisairauksien välinen yhteys. Suomen hammaslaakarilehti.  ISSN 0355-4090.  3, s 24- 29
• Haugen, Håvard Jostein; Qasim, Syed Saad Bin; Matinlinna, Jukka P; Vallittu, Pekka K. & Nogueira, Liebert Parreiras (2020). Nano-CT as tool for characterization of dental resin composites. Scientific Reports.  ISSN 2045-2322.  10 . doi: 10.1038/s41598-020-72599-y Fulltekst i vitenarkiv. Vis sammendrag

  Technological advances have made it possible to examine dental resin composites using 3D nanometer resolution. This investigation aims to characterize existing dental nano-hybrid and micro-hybrid resin composites through comparing and contrasting nano-computed tomography (nano-CT) with micro-CT and high-resolution SEM images. Eight commercially available and widely used dental resin composites, 2 micro-hybrid and 6 nano-hybrid were researched. Cured samples were examined and characterized using nano-CT (resolution 450 nm) and compared with micro-CT images (resolution 2 µm). Acquired images were reconstructed and image analysis was carried out to determine porosity and pore morphology. A comprehensive comparison of scanning micrograph images unsurprisingly revealed that the nano-CT images displayed greater detail of the ultrastructure of cured dental resin composites. Filler particle diameters and its volumes were lower when measured using nano-CT, porosity being higher where analysed at higher resolution. There were large variations between the examined materials. Fewer voids were found in Tetric EvoCeram and IPS Empress Direct, the smallest pores being found in Universal XTE and Tetric EvoCeram. Nano-CT was successfully used to investigate the morphology of dental resin composites and showed that micro-CT gives a lower porosity and pore size but overestimates filler particle size. There were large discrepancies between the tested composites. Evidence of porosities and pores within a specimen is a critical finding and it might have a detrimental effect on a material’s clinical performance.

• Magaz, Adrian; Ashton, Mark; Hathout, Rania; Li, Xu; Hardy, John & Blaker, Jonny J. (2020). Electroresponsive silk-based biohybrid composites for electrochemically controlled growth factor delivery. Pharmaceutics.  ISSN 1999-4923.  12(8), s 1- 12 . doi: 10.3390/pharmaceutics12080742 Fulltekst i vitenarkiv.
• Skallevold, Hans Erling & Verket, Anders (2020). Myasthenia gravis og orale utfordringer. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130(8), s 614- 617
• Ellingsen, Jan Eirik; Chvartszaid, David; Estafanous, Emad; Huynh-Ba, Guy; Oates, Thomas; Osswald, Martin & Stanford, Clark (2020). Impact of Tobacco Smoking on Peri-implantitis. International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(4), s 671- 673
• Oates, Thomas; Ellingsen, Jan Eirik; Chvartszaid, David; Huynh-Ba, Guy; Estafanous, Emad; Osswald, Martin & Stanford, Clark (2020). Enhancing Implant Stability with Osseodensification: Remember That Biology Happens!. International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(3), s 457- 460
• Huynh-Ba, Guy; Estafanous, Emad; Ellingsen, Jan Eirik; Oates, Thomas; Osswald, Martin; Chvartszaid, David & Stanford, Clark (2020). Antibiotics and Implant Therapy Revisited. International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(2), s 244- 247
• Estafanous, Emad; Chvartszaid, David; Ellingsen, Jan Eirik; Huynh-Ba, Guy; Oates, Thomas; Osswald, Martin & Stanford, Clark (2020). Effect of Medications/Chemotherapy on Osseointegration. International Journal of Oral & Maxillofacial Implants.  ISSN 0882-2786.  35(1), s 15- 21
• Skoglund, Lasse Ansgar & Vigen, Ellen Christine (2020). Munnskyllemidler mot pandemisk/epidemisk virus – En kort oversikt. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130(8), s 620- 625
• Kallas, Pawel; Kang, Hua; Valen, Håkon; Haugen, Håvard Jostein; Andersson, Martin & Hulander, Mats (2020). Effect of silica nano-spheres on adhesion of oral bacteria and human fibroblasts. Biomaterial Investigations in Dentistry.  7(1), s 134- 145 . doi: 10.1080/26415275.2020.1816175 Fulltekst i vitenarkiv. Vis sammendrag

  Objective This study investigated the effect of surface nano-patterning on adhesion of an oral early commensal colonizer, Streptococcus mitis and the opportunistic pathogen Staphylococcus aureus and human fibroblasts (HDFa) in a laminar flow cell. Methods Nanostructured surfaces were made by functionalizing glass substrates with 40 nm SiO2 nanoparticles. Gradients in nanoparticle surface coverage were fabricated to study the effect of nanoparticle spacing within a single experiment. Bacterial adhesion was investigated after 5 min of contact time by subjecting surfaces to a flow in a laminar flow cell. In addition, to examine the particles effect on human cells, the establishment of focal adhesion and spreading of primary human dermal fibroblasts (HDFa) were investigated after 4 and 24 h. Results Adhesion of both S. aureus and S. mitis decreased on surfaces functionalized with nanoparticles and coincided with higher nanoparticle surface coverage on the surface. Both strains were tested on three separate surfaces. The regression analysis showed that S. mitis was influenced more by surface modification than S. aureus. The establishment of focal adhesions in HDFa cells was delayed on the nanostructured part of the surfaces after both 4 and 24 h of culturing. Significance In the current manuscript, we have used a flow cell to investigate the effect of nanotopographies on S. aureus and S. mitis adhesion. The present findings are of relevance for design of future implant and prostheses surfaces in order to reduce adhesion of bacteria.

• Aqrawi, Lara Adnan; Jensen, Janicke Cecilie Liaaen; Fromreide, Siren; Galtung, Hilde Kanli & Skarstein, Kathrine (2020). Expression of NGAL-specific cells and mRNA levels correlate with inflammation in the salivary gland, and its overexpression in the saliva, of patients with primary Sjögren’s syndrome. Autoimmunity.  ISSN 0891-6934.  s 1- 11 . doi: 10.1080/08916934.2020.1795140 Fulltekst i vitenarkiv.
• Qasim, Saad B; Nogueira, Liebert Parreiras; Amr S, fawzy & Daood, Umer (2020). The Effect of Cross-linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regeneration. AAPS PharmSciTech.  ISSN 1530-9932. . doi: 10.1208/s12249-020-01708-x Fulltekst i vitenarkiv. Vis sammendrag

  Innovative strategies for periodontal regeneration have been the focus of research clusters across the globe for decades. In order to overcome the drawbacks of currently available options, investigators have suggested a novel concept of functionally graded membrane (FGM) templates with different structural and morphological gradients. Chitosan (CH) has been used in the past for similar purpose. However, the composite formulation of composite and tetracycline when cross-linked with glutaraldehyde have received little attention. Therefore, the purpose of the study was to investigate the drug loading and release characteristics of novel freeze gelated chitosan templates at different percentages of glutaraldehyde. These were cross-linked with 0.1 and 1% glutaraldehyde and loaded with doxycycline hyclate. The electron micrographs depicted porous morphology of neat templates. After cross-linking, these templates showed compressed ultrastructures. Computerized tomography analysis showed that the templates had 88 to 92% porosity with average pore diameter decreased from 78 to 44.9 μm with increasing concentration. Fourier transform infrared spectroscopy showed alterations in the glycosidic segment of chitosan fingerprint region which after drug loading showed a dominant doxycycline spectral composite profile. Interestingly, swelling profile was not affected by cross-linking either at 0.1 and 1% glutaraldehyde and template showed a swelling ratio of 80%, which gained equilibrium after 15 min. The drug release pattern also showed a 40 μg/mL of release after 24 h. These doxycycline-loaded templates show their tendency to be used in a functionally graded membrane facing the defect site.

• Daood, Umer; Omar, Hanan; Qasim, Saad B; Nogueira, Liebert Parreiras; Pichika, Malikarjuna; Mak, Kit-Kay; Steier, Liviu; CKY, Yiu; Lin, Seow Liang & Fawzy, Amr S. (2020). New antimicrobial and collagen crosslinking formulated dentin adhesive with improved bond durability. Journal of The Mechanical Behavior of Biomedical Materials.  ISSN 1751-6161. . doi: 10.1016/j.jmbbm.2020.103927 Vis sammendrag

  Objective Here we describe a novel formulation, based on quaternary ammonium (QA) and riboflavin (RF), which combines antimicrobial activities and protease inhibitory properties with collagen crosslinking without interference to bonding capabilities, was investigated. Methods Experimental adhesives modified with different fractions of dioctadecyldimethyl ammonium bromide quaternary ammonium and riboflavin (QARF) were formulated. Dentine specimens were bonded to resincomposites with control or the experimental adhesives to be evaluated for bond strength, interfacial morphology, micro-Raman analysis, nano-CT and nano-leakage expression. In addition, the antibacterial and biocompatibilities of the experimental adhesives were investigated. The endogenous proteases activities and their molecular binding-sites were studied. Results Modifying the experimental adhesives with QARF did not adversely affect micro-tensile bond strength or the degree of conversion along with the demonstration of anti-proteases and antibacterial abilities with acceptable biocompatibilities. In general, all experimental adhesives demonstrated favourable bond strength with increased and improved values in 1% QARF adhesive at 24 h (39.2 ± 3.0 MPa) and following thermocycling (34.8 ± 4.3 MPa). Significance It is possible to conclude that the use of QARF with defined concentration can maintain bond strength values when an appropriate protocol is used and have contributed in ensuring a significant decrease in microbial growth of biofilms. Incorporation of 1% QARF in the experimental adhesive lead to simultaneous antimicrobial and anti-proteolytic effects with low cytotoxic effects, acceptable bond strength and interfacial morphology.

• Tvilde, Britt Nygard; Virtanen, Jorma; Bletsa, Athanasia; Graue, Anne Marit; Skaare, Anne & Skeie, Marit Slåttelid (2020). Dental erosive wear in primary teeth among five-year-olds–Bergen, Norway. Acta Odontologica Scandinavica.  ISSN 0001-6357.  s 1- 7 . doi: 10.1080/00016357.2020.1791357 Fulltekst i vitenarkiv.
• Borie, Manon; Lecloux, Geoffrey; Bosshardt, Dieter D.; Barrantes Bautista, Alejandro; Haugen, Håvard Jostein; Lambert, France & Bacevic, Miljana (2020). Peri-Implant Soft Tissue Integration in Humans – Influence of Materials: A study protocol for a randomised controlled trial and a pilot study results. Contemporary Clinical Trials Communications.  ISSN 2451-8654. . doi: 10.1016/j.conctc.2020.100643 Fulltekst i vitenarkiv. Vis sammendrag

  Background : Recently, there has been a growing interest in mucointegration as the formation of an early and long-standing soft tissue barrier seems essential for both the initial healing and long-term implant survival. Aim: To develop an experimental method to characterize the mucointegration of different transgingival materials (titanium (Ti), polyetheretherketone (PEEK), polymethylmethacrylate (PMMA), zirconia (Zi), polymer infiltrated ceramic network (PICN), cobalt-chrome (Co-Cr), and lithium disilicate (LD)) in a human model. Methods / Design : The study is designed as a multi-part randomized controlled clinical trial. Ninety bone level Straumann implants will randomly receive an experimental, custom-made abutment to allow for the removal of the abutment together with the surrounding soft tissues using a punch biopsy device at 8 weeks of healing (10 per material). The specimens will be further processed for non‐decalcified histology, followed by histomorphometric analysis. The same protocol will be used for additional 90 implants-abutments, but during harvesting, soft tissues will be separated from the abutment and processed for immunohistochemistry in order to study tissue inflammation and vascularization, while the abutments will undergo SEM analysis. Additionally, in vitro analyses, including SEM and profilometry, will be performed in order to characterize surface topography of all experimental materials. Conclusion : The limited number of pilot samples presented herein indicate that the use of custommade abutments in humans is a reproducible method to study peri-implant soft tissue integration. This further intensifies the rationale to compare different abutment materials, used as transgingival components in daily practice, under the same conditions.

• Grytten, Jostein Ivar & Skau, Irene (2020). Educational inequalities in access to fixed prosthodontic treatment in Norway. Causal effects using the introduction of a school reform as an instrumental variable. Social Science and Medicine.  ISSN 0277-9536.  260 . doi: 10.1016/j.socscimed.2020.113105 Fulltekst i vitenarkiv.
• Wigen, Tove Irene & Wang, Nina Johanne (2020). Characteristics of teenagers who use dental floss. Community Dental Health.  ISSN 0265-539X. . doi: 10.1922/CDH_00006Wigen05 Fulltekst i vitenarkiv.
• Sæthre-Sundli, Helene Borghild; Wang, Nina Johanne & Wigen, Tove Irene (2020). Do enamel and dentine caries at 5 years of age predict caries development in newly erupted teeth? A prospective longitudinal study. Acta Odontologica Scandinavica.  ISSN 0001-6357.  78(7), s 509- 514 . doi: 10.1080/00016357.2020.1739330 Fulltekst i vitenarkiv.
• Mengjun, Xue; Sampath, Janani; Gebart, Rachel; Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter; Pfaendtner, Jim & Drobny, Gary (2020). Studies of dynamic binding of amino acids to TiO2 nanoparticle surfaces by Solution NMR and Molecular Dynamics Simulations. Langmuir.  ISSN 0743-7463. . doi: 10.1021/acs.langmuir.0c01256 Fulltekst i vitenarkiv. Vis sammendrag

  Adsorption of biomolecules onto material surfaces involves a potentially complex mechanism where molecular species interact to varying degrees with a heterogeneous material surface. Surface adsorption studies by atomic force microscopy (AFM), Sum Frequency Generation (SFG) spectroscopy, and solid state NMR (ssNMR), detect the structures and interactions of biomolecular species that are bound to material surfaces and which, in the absence of a solid liquid interface, do not exchange rapidly between surface-bound forms and free molecular species in bulk solution. Solution NMR has the potential to complement these techniques by detecting and studying transiently bound biomolecules at the liquid-solid interface. Herein we show that dark-state exchange saturation transfer (DEST) NMR experiments on gel-stabilized TiO2 nanoparticle (NP) samples detect several forms of biomolecular adsorption onto titanium (IV) oxide surfaces. Specifically, we use the DEST approach to study the interaction of amino acids arginine (Arg), lysine (Lys), leucine (Leu), alanine (Ala), and aspartic acid (Asp) with TiO2 rutile nanoparticle surfaces. Whereas Leu, Ala, and Asp display only a single weakly interacting form in the presence of TiO2 nanoparticles , Arg and Lys displayed at least two distinct bound forms: a species that is surface bound and retains a degree of reorientational motion, and a second more tightly bound form characterized by broadened DEST profiles upon addition of TiO2 nanoparticles. Molecular Dynamics simulations indicate different surface bound states for both Lys and Arg depending on the degree of TiO2 surface hydroxylation, but only a single bound state for Asp regardless of the degree of surface hydroxylation, in agreement with results obtained from analysis of DEST profiles.

• Haugen, Håvard Jostein; Marovic, Danijela; Par, Matej; Thieu, Minh Khai Le; Reseland, Janne Elin & Johnsen, Gaute Floer (2020). Bulk Fill Composites Have Similar Performance to Conventional Dental Composites. International Journal of Molecular Sciences.  ISSN 1422-0067.  21(15), s 5136 . doi: 10.3390/ijms21145136 Fulltekst i vitenarkiv. Vis sammendrag

  The aim of the study was to perform comprehensive characterization of two commonly used bulk fill composite materials (SDR Flow (SDR) and Filtek™ Bulk Fill Flowable Restorative (FBF) and one conventional composite material (Tetric EvoCeram; TEC). Eleven parameters were examined: flexural strength (FS), flexural modulus (FM), degree of conversion, depth of cure, polymerisation shrinkage (PS), filler particle morphology, filler mass fraction, Vickers hardness, surface roughness following simulated toothbrush abrasion, monomer elution, and cytotoxic reaction of human gingival fibroblasts, osteoblasts, and cancer cells. The degree of conversion and depth of cure were the highest for SDR, followed by FBF and TEC, but there was no difference in PS between them. FS was higher for bulk fill materials, while their FM and hardness were lower than those of TEC. Surface roughness decreased in the order TEC→SDR→FBF. Bisphenol A-glycidyl methacrylate (BisGMA) and urethane dimethacrylate were found in TEC and FBF eluates, while SDR released BisGMA and triethylene glycol dimethacrylate. Conditioned media accumulated for 24h from FBF and TEC were cytotoxic to primary human osteoblasts. Compared to the conventional composite, the tested bulk fill materials performed equally or better in most of the tests, except for their hardness, elastic modulus, and biocompatibility with osteoblasts.

• Schröder, Maria; Riksen, Elisabeth Aurstad; He, Jianying; Skallerud, Bjørn Helge; Møller, Mona Elisabeth; Lian, Aina-Mari; Syversen, Unni & Reseland, Janne Elin (2020). Vitamin K2 modulates vitamin D induced mechanical properties of human 3D bone spheroids in vitro. JBMR Plus.  ISSN 2473-4039.  4(9) . doi: 10.1002/jbm4.10394 Fulltekst i vitenarkiv.
• Rahmati, Maryam; Frank, Matthias Johannes; Walter, Martin Sebastian; Monjo Cabrer, Marta; Satué, María; Reseland, Janne Elin; Lyngstadaas, Ståle Petter & Haugen, Håvard Jostein (2020). Osteoimmunomodulatory effects of enamel matrix derivate and strontium coating layers: A short- and long-term in vivo study. ACS Applied Bio Materials (AABM).  ISSN 2576-6422.  3, s 5169- 5181 . doi: 10.1021/acsabm.0c00608 Fulltekst i vitenarkiv. Vis sammendrag

  Over the past few years, surface modification of implant surface has gained substantial attention as a promising solution to avoid the failure of biomaterials after implantation. Although researchers suggest several strategies for surface functionalization of titanium based implants, only few studies have compared the osteoimmunomodulatory effects of ionic nanostructure and biofunctionalization in the same biological model. Enamel matrix derivate (EMD) and strontium are both known for their positive influences on bone cell responses. In this study, we functionalized the titanium-zirconium implant surface with EMD and strontium using an electrochemical cathodic polarization method. Afterwards, we evaluated the osteoimmunomodulatory effects of EMD or strontium coated titanium zirconium implants in the tibia of eight Grey Bastard Chinchillas rabbits. We performed 2&3D micro-CT, wound fluid, histologic, and histomorphometric analyses on bone tissues after 4- and 8-weeks implantation. Although the results could indicate some differences between groups regarding the bone quality, there was no difference in bone amount or volume. EMD stimulated higher ALP activity and lower cytotoxicity in wound fluid, as well as a lower expression of inflammatory markers after 8 weeks indicating its osteoimmunomodulatory effects after implantation. Overall, the results suggested that ionic nanostructure modification and biofunctionalization might be useful in regulating the immune responses to implants.

• Rahmati, Maryam; Silva, Eduardo A.; Reseland, Janne Elin; Heyward, Catherine Anne & Haugen, Håvard Jostein (2020). Biological responses to physicochemical properties of biomaterial surface. Chemical Society Reviews.  ISSN 0306-0012.  49(15), s 5178- 5224 . doi: 10.1039/d0cs00103a Fulltekst i vitenarkiv. Vis sammendrag

  Biomedical scientists use chemistry-driven processes found in nature as an inspiration to design biomaterials as promising diagnostic tools, therapeutic solutions, or tissue substitutes. While substantial consideration is devoted to the design and validation of biomaterials, the nature of their interactions with the surrounding biological microenvironment is commonly neglected. This gap of knowledge could be owing to our poor understanding of biochemical signaling pathways, lack of reliable techniques for designing biomaterials with optimal physicochemical properties, and/or poor stability of biomaterial properties after implantation. The success of host responses to biomaterials, known as biocompatibility, depends on chemical principles as the root of both cell signaling pathways in the body and how the biomaterial surface is designed. Most of the current review papers have discussed chemical engineering and biological principles of designing biomaterials as separate topics, which has resulted in neglecting the main role of chemistry in this field. In this review, we discuss biocompatibility in the context of chemistry, what it is and how to assess it, while describing contributions from both biochemical cues and biomaterials as well as the means of harmonizing them. We address both biochemical signal-transduction pathways and engineering principles of designing a biomaterial with an emphasis on its surface physicochemistry. As we aim to show the role of chemistry in the crosstalk between the surface physicochemical properties and body responses, we concisely highlight the main biochemical signal-transduction pathways involved in the biocompatibility complex. Finally, we discuss the progress and challenges associated with the current strategies used for improving the chemical and physical interactions between cells and biomaterial surface.

• Brunetti, Giacomina; Storlino, Giuseppina; Oranger, Angela; Colaianni, Graziana; Faienza, Maria F; Ingravallo, Giuseppe; Di Comite, Mariasevera; Reseland, Janne Elin; Celi, Monica; Tarantino, Umberto; Passeri, Giovanni; Ware, Carl F; Grano, Maria & Colucci, Silvia (2020). LIGHT/TNFSF14 regulates estrogen deficiency‐induced bone loss.. Journal of Pathology.  ISSN 0022-3417.  250, s 440- 451 . doi: https://doi.org/10.1002/path.5385
• Ulmner, Mattias; Sugars, Rachael; Naimi-Akbar, Aron; Suslu, Safiyye; Reseland, Janne Elin; Kruger-Weiner, Carina & Lund, Bodil Kristina (2020). Synovial tissue cytokine profile in disc displacement of the temporomandibular joint. Journal of Oral Rehabilitation.  ISSN 0305-182X.  47(10), s 1202- 1211 . doi: 10.1111/joor.13051 Fulltekst i vitenarkiv.
• Torgersbråten, Nina Ellen; Stenvik, Arild & Espeland, Lisen Vivienne (2020). Patient satisfaction after orthognathic surgery: a 3 year follow-up of 60 high-angle Class II individuals. European Journal of Orthodontics.  ISSN 0141-5387. . doi: 10.1093/ejo/cjaa038
• Fardal, Øystein; Skau, Irene & Grytten, Jostein Ivar (2020). Familial tendency as a determinant of tooth loss during long-term periodontal therapy. Journal of Clinical Periodontology.  ISSN 0303-6979.  47(2), s 213- 222 . doi: 10.1111/jcpe.13219 Fulltekst i vitenarkiv.
• Qasim, Syed Saad B.; Nogueira, Liebert Parreiras; Fawzy, Amr S. & Daood, Umer (2020). The Effect of Cross-linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regeneration. AAPS PharmSciTech.  ISSN 1530-9932.  21(5) . doi: 10.1208/s12249-020-01708-x

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• Sjilov, Axel; Gustafsson, Miriam K. & Syversen, Unni (2020). Should we be concerned about the vitamin A status among Norwegian pregnant women?.
• Torgersbråten, Nina Ellen; Espeland, Lisen Vivienne & Stenvik, Arild (2020). Patient satisfaction after orthognathic surgery: a 3 year follow-up of 60 high-angle Class II individuals. European Journal of Orthodontics.  ISSN 0141-5387. . doi: 10.1093/ejo/cjaa038
• Torgersbråten, Nina Ellen; Stenvik, Arild & Espeland, Lisen Vivienne (2020). A comparison of Class II open bite correction by maxillary or mandibular surgery. American Journal of Orthodontics and Dentofacial Orthopedics.  ISSN 0889-5406. . doi: 10.1016/j.ajodo.2019.05.018.
• Diep, My Tien; Jensen, Janicke Liaaen; Sødal, Anne Thea Tveit; Young, Alix; Skudutyte-Rysstad, Rasa & Hove, Lene Hystad (2020). Are Xerostomia and Hyposalivation Common Problems Among 65-Year-Olds?. Caries Research.  ISSN 0008-6568.  54(4), s 449- 450 . doi: 10.1159/000511860
• Weber, Florian; Sagstuen, Einar & Tiainen, Hanna (2020). Chemistry of Silica-Phenolic Networks.
• Skaare, Anne B; Herlofson, Bente Brokstad & Søland, Tine Merete (2020). Orofacial granulomatose og Crohns sykdom – et klinisk kasus og en litteraturgjennomgang. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130(11), s 914- 920
• Sødal, Anne Thea Tveit; Singh, Preet Bano; Diep, My Tien & Hove, Lene Hystad (2020). Prevalence of Smell, Taste, Trigeminal Disorders and Quality of Life Among 65-Year-Olds in Oslo. Caries Research.  ISSN 0008-6568.  54(4), s 449- 449 . doi: 10.1159/000511860
• Herlofson, Bente Brokstad (2020). Pasienter på benmodulerende behandling og osteonekrose - med fokus på myelomatose.
• Herlofson, Bente Brokstad (2020). Jaw osteomyelitis/osteonecrosis - clinical presentation, diagnosis, therapy.
• Steffensen, Inger-Lise; Alexander, Jan; Binderud, Mona-Lise; Bruzell, Ellen Merete; Dahl, Knut Helkås; Granum, Berit; Herlofson, Bente Brokstad; Hetland, Ragna Bogen; Husøy, Trine; Paulsen, Jan Erik; Rohloff, Jens; Thrane, Vibeke & Wicklund, Trude (2020). Risk assessments of aspartame, acesulfame K, sucralose and benzoic acid from soft drinks, "saft", nectar and flavoured water. European Journal of Nutrition and Food Safety.  ISSN 2347-5641.  12(8), s 66- 68 . doi: 10.9734/EJNFS72020/v12i830265
• Herlofson, Bente Brokstad (2020). Kreftpasienter på antiresorptiv behandling med risiko for utvikling av osteonekrose i kjevene.
• Herlofson, Bente Brokstad (2020). Pasienter på benmodulerende behandling og osteonekrose.
• Qasim, Syed Saad Bin; Nogueira, Liebert Parreiras; Fawzy, Amr S. & Daood, Umer (2020). Correction to: the Effect of Cross-Linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regeneration (AAPS PharmSciTech, (2020), 21, 5, (173), 10.1208/s12249-020-01708-x). AAPS PharmSciTech.  ISSN 1530-9932.  21(7) . doi: 10.1208/s12249-020-01778-x
• Jensen, Janicke Liaaen (2020). Integrated oral and general health.
• Jensen, Janicke Liaaen (2020). Eldres munnehelse går under radaren. forskning.no.
• Jensen, Janicke Liaaen (2020). Hvordan påvirker revmatiske sykdommer generelt, og Sjögrens syndrom spesielt, oral helse?.
• Suslu, Safiyye; Høgestøl, Ingvild Kristine; Eriksen, Erik Fink & Reseland, Janne Elin (2020). Changes in protein profile in bone marrow extracts before and one year after gastric bypass surgery. Bone Reports.  ISSN 2352-1872.  13 . doi: https://doi.org/10.1016/j.bonr.2020.100368
• Skoglund, Lasse Ansgar (2020). NTFs nye obligatoriske etterutdanning: Svar til Sigbjørn Løes. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130(9)
• Skoglund, Lasse Ansgar (2020). NTFs nye obligatoriske etterutdanning: En liten kommentar. Den norske tannlegeforenings tidende.  ISSN 0029-2303.  130, s 734- 735

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