Understanding salivary gland function

“Understanding Salivary Gland Function” is a strategic project aiming at a deeper understanding of salivary gland function with initial emphasis on primary Sjögren’s syndrome (pSS).

Profilbilde av professor Janicke Liaaen Jensen
Project Leader, Professor Janicke Liaaen Jensen: j.c.l.jensen@odont.uio.no

Main goals of the research project

  • To improve the situation for all groups of patients suffering from dry mouth symptoms and problems, through increased clinical awareness, accurate diagnosis, and better treatment aimed at improving salivary secretion and associated mucous membrane changes and infections.
  • To elucidate biomarkers for pSS using advanced investigations of tears and saliva from pSS patients.

Background

SS is an autoimmune connective tissue disorder that mainly affects women from approximately 50 years of age. SS involves dry mouth, dryness of the eyes, fatigue, pain in muscles and joints, significantly impaired quality of life and increased morbidity. The disease is the subject of great attention among clinicians and researchers; nevertheless, the cause is unknown.

It is of significant interest to the individual patient and for the patient group as a whole to achieve improved diagnostics and, thus, possible therapies.

However, decreased salivation and dry mouth are common complaints in the population, and may be caused by drug side effects, irradiation against salivary glands, or diseases in salivary glands. The frequency of dry mouth increases with age, mainly due to greater morbidity and drug use in the elderly population.

Very low salivary secretion can impair oral health in the form of dental decay and wear, fungal infections, damage to the mucous membranes, difficulties with speaking and swallowing, and reduced general quality of life.

The Dry Mouth Clinic

The establishment of the Dry Mouth Clinic is an essential element of the overall project. During the fall of 2015, the Dry Mouth Clinic was established at the Institute of Clinical Dentistry through collaboration between resource persons from the departments of surgery, conservative dentistry and gerodontology.

From left: Bente Brokstad Herlofson, Lene Hystad Hove, Morten Rykke, Janicke Liaaen Jensen and Preet Bano Singh. Front from left: Alix Young Vik and Shermin Rusthen.

Patients and external collaborators

The project requires access to patients with a well-established pSS diagnosis and high competence in the field of dry eyes. pSS patients were identified in the Norwegian Systemic Connective Tissue Disease and Vasculitis Register (NOSVAR), the National Hospital, led by Øyvind Palm and re-examined before referral. In addition, healthy age- and gender matched controls (negative controls) and patients with sicca symptoms and findings not fulfulling pSS-criteria (non-SS) were examined.The Dry Eye Clinic, led by Tor Paaske Utheim, is the Nordic region's only special clinic dedicated to diagnosis and treatment of dry eyes, and performs advanced examinations of eyes and tears using state-of-the-art technology. Furthermore, in collaboration with both Oslo University Hospital and Department of Biosciences, saliva and tears were closely analysed by size exclusion chromotography and proteomics in order to identify biomarkers for SS that later can be used in diagnosis and therapy. Current research focuses on analyses of cytokines in saliva and tears.

Project progress

By June 2018, 54 well-characterized SS patients, 56 healthy controls and 25 non-SS patients were recruited and examined at the Dry Mouth Clinic. They were all subjected to questionnaires on general and oral quality of life, as well as xerostomia. Patient history data were collected, the oral mucosa and teeth were closely examined, saliva was collected, and tests on smell and taste were performed. All were also extensively examined at the Dry Eye Clinic.

Future aspects

Studies on pSS will continue, but with time the project will include all categories of patients with dry mouth and/or dry eyes; head and neck cancer, multimorbidity, and multipharmacy. New treatment options will be eplored, i.e. nanoparticles in collaboration with Professor Marianne Hiorth, School of Pharmacy.

Contact information

Project leader Janicke Liaaen Jensen: jljensen@odont.uio.no

 

Publications

Project abstracts

  1. Amin Homayouni, Behzod Tashbayev, Shermin Rusthen, Lene Hystad Hove, Morten Rykke, Preet Bano Singh, Bente Brokstad Herlofson, Janicke Liaaen Jensen, Alix Young (2018): Can saliva secretion affect oral malodour and oral health-related quality of life? ORCA, Copenhagen,July 2018.
  2. Rusthen S, Kristoffersen AK, Young A, Galtung HK, Enersen M, Jensen JL (2018):
    The Salivary Microbiota Profile in Reduced and Normal Salivation. IADR; London, July 2018.
  3. Tashbayev, Behzod; Chen, Xiangjun; Utheim, Øa; Rusthen, Shermin; Young, Alix; Herlofson, Bente Brokstad; Hove, Lene Hystad; Singh, Preet Bano; Rykke, Morten; Aqrawi, Lara Adnan; Utheim, Tor Paaske; Garen, Torhild Oddveig; Palm, Øyvind & Jensen, Janicke Liaaen (2018). Primary Sjögren´s Syndrome and Non-Sjögren´s Sicca Syndrome Patients; unexpected differences in symptoms and findings of dry eye disease, dry mouth, and oral health related quality of life. 14 International Symposium on Sjögren’s syndrome, Washington, April 2018.
  4. Tashbayev, Behzod; Rusthen, Shermin; Chen, X; Utheim, Tor Paaske; Utheim, Øygunn Aass; Young, Alix; Herlofson, Bente Brokstad; Aqrawi, Lara Adnan; Rykke, Morten; Hove, Lene Hystad; Palm, Øyvind & Jensen, Janicke Liaaen (2017). Dry Mouth and Dry Eye Examinations of a Norwegian Cohort of Primary Sjögren´s Syndrome Patients, ORCA, Oslo, July 2017.
  5. Rusthen, Shermin; Young, Alix; Preet, Bano; Tashbayev, Behzod; Garen, Torhild Oddveig; Aqrawi, Lara A.; Rykke, Morten; Hove, Lene Hystad; Herlofson, Bente Brokstad; Palm, Øyvind & Jensen, Janicke Liaaen (2017). Is Health-related Quality of Life Correlated to Oral Status in Primary Sjögren’s Syndrome Patients? ORCA, Oslo, July 2017.
  6. Jensen, Janicke Liaaen; Skarstein, Kathrine.
    Salivary Gland Biopsies in Sjögren’s Syndrome: Focus Score is Related to Age. XIII International Symposium on Sjögren's Syndrome; 2015-05-19 - 2015-05-22
  7. Jensen, Janicke Liaaen; Helgeland, Lars; Skarstein, Kathrine. Sjögren’s syndrome: Salivary gland biopsies and risk of lymphoma development. IADR; 2015-03-11 - 2015-03-14
  8. Aqrawi LA , Ivanchenko M, Björk A, Ramirez J, Kvarnström M, Jensen JL, Skarstein K, Wahren-Herlenius M (2016). Expression of the chemokine receptor CXCR5 is decrease d in the periphery of primary Sjögren’s syndrome patients. ACR, US.
  9. Jensen, Janicke Liaaen; Utheim, Øygunn Aass; Chen, Xiangjun; Thiede, Bernd; Aqrawi, Lara Adnan; Rusthen, Shermin; Young, Alix; Utheim, Tor Paaske & Galtung, Hilde (2016). Searching for Disease Biomarkers for Primary Sjögren’s Syndrome Based on Oral and Ocular Patient Examinations. BATTS, Oklahoma City, US.
  10. Young, Alix; Rusthen, Shermin; Aqrawi, Lara Adnan; Rykke, Morten; Hove, Lene Hystad; Herlofson, Bente Brokstad; Singh, Preet Bano; Palm, Øyvind & Jensen, Janicke Liaaen (2016). Dental health status of patients diagnosed with primary Sjögren's Syndrome. ORCA, Athens, Greece.
  11. Rusthen, Shermin; Singh, Preet Bano; Young, Alix; Aqrawi, Lara Adnan; Rykke, Morten; Hove, Lene Hystad; Herlofson, Bente Brokstad; Palm, Øyvind & Jensen, Janicke Liaaen (2016). Oral quality of life, salivary, gustatory and olfactory status in patients with primary Sjögren’s syndrome. ORCA, Athens Greece.
  12. Hove, Lene Hystad; Aqrawi, Lara Adnan; Rusthen, Shermin; Young, Alix; Rykke, Morten; Herlofson, Bente Brokstad; Singh, Preet Bano; Palm, Øyvind; Utheim, Tor Paaske & Jensen, Janicke Liaaen (2016). Establishment of a Dry Mouth Clinic at the Faculty of Dentistry, University of Oslo, Norway. ORCA, Athens Greece.
  13. Singh, Preet Bano; Rusthen, Shermin; Aqrawi, Lara A.; Young, Alix; Rykke, Morten; Hove, Lene Hystad; Herlofson, Bente Brokstad; Palm, Øyvind & Jensen, Janicke Liaaen (2016). Chemosensory disorders, burning mouth sensation, and halitosis in patients with Sjögren’s syndrome. Association for Chemoreception Sciences, Annual meeting, Florida, US.

 

Invited presentations

Jensen, Janicke Liaaen (2018). 

Munntørrhet, en utfordring særlig hos voksne pasienter. Tannpleierforeningens 50-årsjubileum, Oslo mai 2018.

Herlofson, Bente Brokstad; Jensen, Janicke Liaaen & Singh, Preet Bano (2017). 

Lite spytt, blottlagt bein og vond smak, hva kan det være? NTF’s landsmøteOslo, nov 2017.

Jensen, Janicke Liaaen & Skarstein, Kathrine (2017).

Understanding salivary gland function. Presentasjon av prosjektet for revmatologer.

Jensen, Janicke Liaaen (2017).

Presentation of the Dry Mouth Clinic at Faculty of Dentistry, University of Oslo, Norway. SFOMK, Helsinki, juni 2017.

Jensen, Janicke Liaaen (2017).

Hyposalivation in the elderly. A report from the Dry Mouth Clinic in Oslo, Norway.

Jensen, Janicke Liaaen. ORCA, Oslo, juli 2017.
The newly established Dry Mouth Clinic at the Faculty of Dentistry; potential for research. Harvard Day; 2016-06-07

Jensen, Janicke Liaaen.
Defining the Heterogeneity of Sjögren's Syndrome Patients. Gjesteforelesning ved Forsyth Institute; 2015-03-11 - 2015-03-11

Jensen, Janicke Liaaen.
Establishment of a Dry Mouth Clinic and plans for a future EU application. Skandinavisk nettverksmøte Sjögrens syndrom; 2015-09-22

Jensen, Janicke Liaaen.
Sjögrens syndrom, sett med en oral kirurgs øyne. Internundervisning for revmatologer; 2015-02-04 - 2015-02-04

Jensen, Janicke Liaaen.
Sjögrens syndrom, sett med en oral kirurgs øyne. Internundervisning ved Revmatologisk avdeling Rikshospitalet; 2015-01-22

Jensen, Janicke Liaaen.
Munntørrhet. Konsekvenser for munnhulen, generell helse, livskvalitet og samfunnet. Landsmøte i Tannlegeforeningen; 2014-10-16

 

Project publications

  1. Aqrawi, Lara Adnan; Jensen, Janicke Liaaen; Øijordsbakken, Gunnvor; Ruus, Ann-Kristin; Nygård, Ståle; Holden, Marit; Jonsson, Roland; Galtung, Hilde & Skarstein, Kathrine (2018). Signalling pathways identified in salivary glands from primary Sjögren's syndrome patients reveal enhanced adipose tissue development. Autoimmunity.  ISSN 0891-6934.  51(3), s 135- 146 . doi: 10.1080/08916934.2018.1446525
  2. Nesvold, Marianne; Jensen, Janicke Liaaen; Hove, Lene Hystad; Singh, Preet Bano; Young, Alix; Palm, Øyvind; Andersen, Lene Frost; Carlsen, Monica Hauger & Iversen, Per Ole (2018). Dietary intake, body composition and oral health parameters among female patients with primary Sjögren’s syndrome. Nutrients.  ISSN 2072-6643.  10(866) . doi: 10.3390/nu10070866
  3. Tashbayev, Behzod; Rusthen, Shermin; Young, Alix; Herlofson, Bente Brokstad; Hove, Lene Hystad; Singh, Preet Bano; Rykke, Morten; Aqrawi, Lara A.; Chen, Xiangjun; Utheim, Øygunn Aass; Utheim, Tor Paaske; Palm, Øyvind & Jensen, Janicke Liaaen (2017). Comprehensive Oral and Ocular Evaluation of Patients with Primary Sjögren’s Syndrome.. Scientific Reports.  ISSN 2045-2322. . doi: doi: 10.1038/s41598-017-10809-w
     
  4. Rusthen, Shermin; Young, Alix; Herlofson, Bente Brokstad; Aqrawi, Lara A.; Rykke, Morten; Hove, Lene Hystad; Palm, Øyvind; Jensen, Janicke Liaaen & Singh, Preet Bano (2017). Oral disorders and oral health-related quality of life in patients with primary Sjögren’s Syndrome.. European Journal of Oral Sciences.  ISSN 0909-8836.  00, s 1- 7
     
  5. Chen, Xiangjun; Utheim, Øygunn Aass; Xiao, J.; Stojanovic, A; Tashbayev, Behzod; Jensen, Janicke Liaaen & Utheim, Tor Paaske (2017). Meibomian gland features in a Norwegian cohort of patients with primary Sjögren´s syndrome.. PLoS ONE.  ISSN 1932-6203. . doi: 10.1371/journal.pone.0184284
     
  6. Aqrawi, Lara A.; Galtung, Hilde; Vestad, Beate; Øvstebø, Reidun; Thiede, Bernd; Rusthen, Shermin; Young, Alix; Guerreiro, Eduarda; Utheim, TP; Chen, X; Utheim, Øa; Palm, Øivind & Jensen, Janicke Liaaen (2017). Identification of potential saliva and tear biomarkers in primary Sjögren’s syndrome, utilising the extraction of extracellular vesicles and proteomics analysis. Arthritis Research & Therapy.  ISSN 1478-6362. . doi: 10.1186/s13075-017-1228-x
     
  7. Skarstein K, Aqrawi LA, Øijordsbakken G, Jonsson R, Jensen JL. Adipose tissue is prominent in salivary glands of Sjögren's syndrome patients and appears to influence the microenvironment in these organs. 2016, 20:1-9. [Epub ahead of print]
Tags: saliva, salivary gland, Sjögren's syndrome
Published Jan. 11, 2017 10:28 AM - Last modified Aug. 1, 2018 1:26 PM